Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus (BTV) serotype 6
|
|
Virus Species
* IAH dsRNA
virus collection number |
Serotype
(identification method) |
Place of
original sample eg country town [grid reference] (date original sample was
taken) |
Isolated from
which host species e.g cow, sheep, etc (Date of virus isolation, where, by
whom) |
Position of
storage in reference freezers
(Date added to collection) |
Suppliers
Isolate Designation) |
Adapted to
which cell types Original sample material (Passage history of sample) |
* Original
sample supplied to the collection by person / institution [IAH virus
diagnosis, ISIS sample number]
Comments |
References &
[accession numbers] |
|
RSArrrr/06
|
BTV- 6
(western topotype
-
reference strain) |
Supplied by OVI S. Africa* (25/06/82) |
|
Shelf B3,
Box B3γ,
Rows A1 -B10
(Added to
collection on
20th May 2005)
|
(BTV-6
Ref. Strain)
|
E3/BHK4
[IAH/BHK1]
|
*
Duplicate
samples A1(g) supplied on 19th Dec 2007, to JongChul, Jeong, Ja Yeon
Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City,
KyungKi-Do,440-330, Korea
* Vial B1 (p) A&B sent to Mehdi El Harrak, Biopharma ,
Morocco 29th June 2009
*
Vial B2 sent to
Denis Kolbasov, Russian National
Institute of Veterinary Virology 29th
Sept 2009
*
Vial A sent to Dr Graham Abdeljelil,
Institut Pasteur de Tunis
8th
March 2011
*
|
Vial position A5(g) was
taken out of
Freezer A on 31st
May 2013 and given to
Marcela Gasparini
(Pirbright) to make
reference RNA for use in
Brazil (Corresponding
vial in freezer B
has not been removed)
*
Vial,
B4 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th
April 2013 for sequencing.
Corresponding vial in freezer B was
not removed
* |
|
[Seg-1:GQ506498,
Seg-2:AJ585127,
Seg-3:
GQ506499,
Seg-4: GQ506500,
Seg-5: GQ506501,
Seg-6:
AJ586703,
Seg-7: GQ506502,
Seg-8: GQ506503,
Seg-9: GQ506504,
Seg-10: GQ506505
Maan et al 2007
(3);
Maan et al 2010 (4)]
* |
|
RSAvvvv/06 |
BTV-6 vaccine strain |
S. Africa |
|
|
BTV-6 vaccine strain |
|
|
[Seg-1: GQ506506,
Seg-2: GQ506507,
Seg-3: GQ506508,
Seg-4 GQ506509,
Seg-5: GQ506510,
Seg-6: GQ506511,
Seg-7: GQ506512,
Seg-8: GQ506513,
Seg-9: GQ506514,
Seg-10: GQ506515
Maan et al (2010)] |
|
GER2008/14
|
BTV-6
Identified by BTV-6 specific real time PCR LSI assay
|
Germany
(sample taken
2008)
|
blood from
experimentally infected cattle
|
Shelf B18,
box B18
β
,
rows E1-F10
Added to collection
29th
July 2010
|
from
Animal
No. 15
(12
dpi)
|
[IAH
KC1/BHK1]
Derived from
GER2008/12
|
From
Bernd Hoffmann
[Stars no. A176/08 3]
Received at IAH approximately 12th Dec 2008
Note MTA: Not provided to other parties without
written permission from Bernd Hoffman : also data published from this
strain, must acknowledge and inform Bernd Hoffman
|
|
GER2008/12 |
BTV-6
Identified by BTV-6 specific real time PCR LSI assay
|
Germany
|
blood from
experimentally infected cattle
|
Shelf B18,
box B18
α
,
rows I 1-J 10
(Added to collection
28th
July 2010)
|
Animal
No. 15
(12
dpi)
|
[IAH/KC1])
Derived from
GER2008/05,
used to generate
GER2008/14 |
Bernd Hoffmann
[Stars no. A176/08 3]
Received at IAH approximately 12th Dec 2008 |
Note MTA: Not provided to other parties without
written permission from Bernd Hoffman : also data published from this
strain, must acknowledge and inform Bernd Hoffman
|
|
GER2008/07 |
BTV-6
contains BTV-6 and BTV-8 by real-time and conventional RT-PCR,
but only BTV-6 was isolated : see
GER2008/02
|
Germany
Received at IAH approximately 12th
Dec 2008 |
Positive field cattle blood
|
Shelf B7,
box
B7 α.
rows
I1-J10
(Added to collection
16th Jan 2009) |
Animal No. BH266/2 |
original blood sample |
Bernd Hoffmann
A176/08 2 |
Note MTA: Not provided to other parties without
written permission from Bernd Hoffman : also data published from this
strain, must acknowledge and inform Bernd Hoffman
Used to generate
GER2008/02 |
|
GER2008/06 |
BTV-6
contains BTV-6 and BTV-8 by real-time and conventional RT-PCR,
but only BTV-6 was isolated : see
GER2008/01
|
Germany
Received at IAH approximately 12th
Dec 2008 |
Positive field cattle blood
|
|
Animal No. BH266/1 |
original blood sample |
Bernd Hoffmann
A176/08 1 |
Note MTA: Not provided to other parties without
written permission from Bernd Hoffman : also data published from this
strain, must acknowledge and inform Bernd Hoffman
Used to generate
GER2008/01 |
|
GER2008/05
|
BTV-6
Identified by BTV-6 specific real time PCR LSI
assay
|
Germany
(sample taken
2008)
Received at IAH approximately 21st
Jab 2009
|
blood from
experimentally infected cattle
|
|
Animal No. 15
(12 dpi)
|
Blood sample from
experimentally infected cattle
used to generate isolate
GER2008/04,
GER2008/12
&
GER2008/14
|
Bernd Hoffmann
Note : only 9 vials (P)
[Stars no. A176/08 3]
[
S2: GQ506461, S4: GQ506462,
S5: GQ506463, S6: GQ506464,
S7: GQ506465, S8: GQ506466,
S9: GQ506467 ]
Note MTA: Not provided to other parties without
written permission from Bernd Hoffman : also data published from this
strain, must acknowledge and inform Bernd Hoffman
|
|
GER2008/04 |
BTV-6
Identified by BTV-6 specific real time PCR LSI assay
|
Germany
Received at IAH approximately 12th
Dec 2008 |
blood from experimentally infected cattle
|
Shelf B8,
box
B8 β,
rows
I1-J10
(Added to collection
09th Feb 2009) |
Animal
No. 15
(12 days post infection) |
[IAH/KC2])
Derived from GER2008/05 |
Bernd Hoffmann
[Stars no. A176/08 3] |
Note MTA: Not provided to other parties without
written permission from Bernd Hoffman : also data published from this
strain, must acknowledge and inform Bernd Hoffman |
|
GER2008/03 |
BTV-6
Identified by BTV-6 specific real time PCR LSI assay
|
Germany
Received at IAH approximately 12th
Dec 2008 |
Vero cell culture isolated at FLI Riems |
Shelf B7,
box
B7 β,
rows
A1-B10
(Added to collection
16th Jan 2009) |
Animal No. 15
7 dpi
|
V2 (IAH V1 ) |
Bernd Hoffmann
A176/08 4 |
Note MTA: Not provided to other parties without
written permission from Bernd Hoffman : also data published from this
strain, must acknowledge and inform Bernd Hoffman |
|
GER2008/02 |
BTV-6
Identified by BTV-6 specific real time PCR LSI assay
|
Germany
Received at IAH approximately 12th
Dec 2008 |
Positive field cattle blood
|
Shelf B7,
box
B7 α.
rows
I1-J10
(Added to collection
16th Jan 2009) |
Animal No. BH266/2 |
[IAH/KC1] |
Bernd Hoffmann
A176/08 2 |
Note MTA: Not provided to other parties without
written permission from Bernd Hoffman : also data published from this
strain, must acknowledge and inform Bernd Hoffman
Derived from blood sample
GER2008/07 |
|
GER2008/01 |
BTV-6
Identified by BTV-6 specific real time PCR LSI assay
|
Germany
Received at IAH approximately 12th
Dec 2008 |
Positive field cattle blood
|
Shelf B,
box
B7 α,
rows
G1-H10
(Added to collection
16th Jan 2009) |
Animal No. BH266/1 |
[IAH/KC1] |
Bernd Hoffmann
A176/08 1 |
Note MTA: Not provided to other parties without
written permission from Bernd Hoffman : also data published from this
strain, must acknowledge and inform Bernd Hoffman
Derived from blood sample
GER2008/06 |
|
NET2008/17 |
BTV-6
and
BTV-8
Identified by seg-2 specific RT-PCR |
Netherlands:
Luttenburg |
Cattle
blood
|
Shelf B17,
box B17
γ
,
rows H1-H2
(Added to collection
29th July 2010)
|
|
Blood sample
from cattle
(no
passage
history)
Used to generate
isolate
NET2008/15
|
Supplied by Piet Van Rijn CIDC
Lelystad
[Stars No. A163/08 no 1]
|
ProMed Report
related to BTV-6 vaccine strain
Maan et al (2010)
|
|
NET2008/16 |
BTV-6
Identified by seg-2 specific RT-PCR |
BTV-6
Identified by seg-2 specific RT-PCR |
from
cattle blood
Isolated at
IAH-P
7th Nov 2008
|
|
|
from cattle
blood sample
[IAH/KC2] |
Supplied
by Piet Van Rijn CIDC
Lelystad
[Stars No. A163/08 no 4]
|
ProMed Report
related to BTV-6 vaccine strain
Maan et al (2010)
|
|
NET2008/15 |
BTV-6
and
BTV-8
Identified by seg-2 specific RT-PCR |
Netherlands:
Luttenburg |
from
cattle blood
Isolated at
IAH-P
7th Nov 2008
|
|
|
from cattle
blood sample
[IAH/KC2]
Derived from
blood sample
NET2007/17 |
Supplied
by Piet Van Rijn CIDC
Lelystad
[Stars No. A163/08 no 1]
|
ProMed Report
related to BTV-6 vaccine strain
Maan et al (2010)
|
|
NET2008/14 |
BTV-6
Identified by seg-2 specific RT-PCR |
Netherlands:
Heeton |
from
cattle blood
Isolated at
IAH-P
7th Nov 2008
|
|
|
from cattle
blood sample
[IAH/E1/BHK2] |
Supplied
by Piet Van Rijn CIDC
Lelystad
[Stars No. A163/08 no 3]
|
ProMed Report
related to BTV-6 vaccine strain
Maan et al (2010)
|
|
NET2008/13 |
BTV-6
Identified by seg-2 specific RT-PCR
|
Netherlands:
Barchem |
from
cattle blood
Isolated at
IAH-P
7th
Nov 2008
|
|
|
from cattle
blood sample
[IAH/E1/BHK2] |
Supplied
by Piet Van Rijn CIDC
Lelystad
[Stars No. A163/08 no 4]
|
ProMed Report
related to BTV-6 vaccine strain
Maan et al (2010)
|
|
NET2008/12 |
BTV-6
Identified by seg-2 specific RT-PCR |
Netherlands:
Barchem |
from
cattle blood
Isolated at
IAH-P
18th Nov 2008
|
Shelf
A17,
box A17
γ,
rows A1
–B10
added to collection
20th Nov 2008
|
|
from cattle
blood sample
[IAH KC2/BHK1]
NET2008/08) |
Supplied by Piet
Van Rijn CIDC
Lelystad
[Stars No.
A163/08 no
4] |
ProMed Report
related to BTV-6 vaccine strain
Maan et al (2010)
|
|
NET2008/10 |
BTV-6
Identified by seg-2 specific RT-PCR |
Netherlands:
Barchem |
blood
from cattle
(isolated at
IAH P
7th Nov 2008) |
Shelf
A17,
box A17
beta,
rows C1
–D10
added to collection
7th Nov 2008
|
|
from cattle
blood sample
[IAH/E1/BHK2]
(Derived from
NET2008/08) |
Supplied by Piet
Van Rijn CIDC
Lelystad
[Stars No. A163/08 no 4]
|
ProMed Report
related to BTV-6 vaccine strain
Maan et al (2010)
|
NET2008/08
(virus isolate) |
BTV-6 |
Netherlands 2008 |
|
Shelf A17,
Box A17 alpha,
Rows I 1 to J10 |
|
|
|
NET2008/08
(blood sample) |
BTV-6
Identified by
seg-2 specific RT-PCR and sequence analyses |
Netherlands:
Barchem |
|
Shelf B17,
box B17
γ
,
rows F9
(Added to collection
28th
July 2010)
|
|
Blood sample
from cattle
(no passage
history)
Used to generate isolate
NET2008/10
&
NET2008/12 |
Supplied by Piet
Van Rijn CIDC
Lelystad
[Stars No.
A163/08 no
4]
ProMed Report
related to
BTV-6 vaccine strain
Maan et al (2010)
See also
blood sample NET2008/09
(Mixed
BTV-6 and 8)
and
NET2008/06
(BTV-6
blood sample)
|
|
NET2008/07 |
BTV-6
Identified as BTV-6 by conventional RT-PCR (three primer
sets) and sequence analysis. |
Netherlands:
Heeton
September 2008
|
from cattle blood
Isolated
at
IAH-P
27th Oct
2008 |
Shelf A17,
Box A17 alpha,
rows G12 to H10
&
Shelf B10,
box B10 α,
rows I1 –J10
Added to collection on
18th Sept 2009
|
|
[IAH/KC2]
(Derived from
NET2008/04) |
Supplied by Piet
Van Rijn CIDC
Lelystad
[Stars No.
A163/08 no
3]
|
Seg-2 of this isolate is closely related to that of
the South African reference strain of BTV-6
( >99% identity)
ProMed Report
BTV-6 (vaccine strain)
Maan et al (2010)
|
|
NET2008/06 |
BTV-6
Identified by
seg-2 specific RT-PCR and sequence analyses |
Netherlands:
Heeton |
|
Shelf B17,
box B17
γ
,
rows G9-10
(Added to collection
28th
July 2010)
|
|
Blood sample
from cattle
(no passage
history) |
Supplied by Piet
Van Rijn CIDC
Lelystad
[Stars No.
A163/08 no
3] |
ProMed Report
BTV-6 (vaccine strain)
[
S2:GU550957,
S5: GQ506482,
S6: GQ506483,
S7:
GQ506484,
S8: GQ506485,
S9:
GQ506486,
S10: GQ506487
]
Maan et al (2010) |
|
NET2008/05 |
BTV-6
(to be
confirmed) |
Netherlands:
Heeton
September 2008 |
from cattle blood
Isolated
at
IAH-P
27th Oct
2008 |
Shelf A17,
box A17 α,
rows C1
–F10
Added to collection on
(5th Nov 2008) |
|
[IAH/KC1/BHK1] |
Supplied by Piet
Van Rijn
CIDC
Lelystad
[Stars No.
A163/08 no
3]
ProMed Report
BTV-6 (vaccine strain)
*
Duplicate vials
were removed by Narender
Maan to be sent
to Fort Dodge 31st March 2009.
*
Vial
A17C2(g) A&B sent to Evert Jor,
National
Veterinary Institute Norway,
on 31st March 2009
*
Vial
position D2 was removed and given
to C. Batten IAH-P, on 25th Aug 2009
to grow material for VLA for PCR validation,
Duplicate vial left in Misc box
position H8
*
|
[Seg-1:
GQ506472,
Seg-2:
GQ506473,
Seg-3:
GQ506474,
Seg-4:
GQ506475,
Seg-5:
GQ506476,
Seg-6:
GQ506477,
Seg-7:
GQ506478,
Seg-8:
GQ506479,
Seg-9:
GQ506480,
Seg-10:
GQ506481
Maan et al (2010)
(4)]
|
|
NET2008/04 |
BTV-6
Identified as BTV-6 by conventional RT-PCR (three primer
sets) and sequence analysis. |
Netherlands:
Heeton
September 2008
|
from cattle blood
Isolated
at
IAH-P
27th Oct
2008 |
Shelf A17,
box A17 α,
rows A1
–B9
|
|
[IAH/KC1]
Used to generate
25th Jan 2010
(NET2008/07)
Seg-2 of this isolate is very closely related to that of
the South African reference strain of BTV-6
( >99% identity)
|
Supplied by Piet
Van Rijn
CIDC Lelystad
[Stars No. A163/08 no 3]
Vials A8 were sent to
Juan de Plana Fort Dodge, in Spain, 21st Jan 2009.
*
Vial A9
(duplicate) sent Merial Lyon France
(Pascal Hudelet) 3rdSept 2009
*
Additional vials ,
were added to restock positions rows A1 –B8 and B10 25th Jan 2010 .
*
Vial, A1 from Freezer A was given to Elizabeth Morecroft (Pirbright)
26th April 2013 for sequencing.
Corresponding vial in
freezer B was not removed
*
ProMed Report
BTV-6 (vaccine strain)
;
Maan et al (2010)
[
S1: GQ506468, S2:GU550956,
S3: GQ506469, S4: GQ506470,
S7: GQ506471 ]
|
|
USA2006/02 |
BTV-6
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-6 antisera) |
Okeechobee County, FL,
USA
(sample taken
11th Oct 2006) |
From cattle
(no clinical
history)
Date of virus
preparation in USA
not given |
Shelf E3,
box E3β,
rows I1 – J10
Added to
collection
17th Sept 2007 |
06-461278-5
(tube
labelled: 461278-5;
IVD BHK/1;
10-30-06) |
From blood
E1/BHK1
[IAH/ BHK3] |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA
|
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
Blood
originally taken for testing to clear animals for export to Brazil
|
|
USA2006/01 |
BTV-6
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-6 antisera) |
Okeechobee County, FL,
USA
(sample taken
11th Oct 2006) |
From cattle
(no clinical
history)
Date of virus
preparation in USA
not given |
Shelf E3,
box E3β,
rows A1 – B10
Added to
collection
17th Sept 2007 |
06-461278-3
(tube
labelled: 461278-3;
IVD BHK/1;
10-30-06) |
From blood
E1/BHK1
[IAH/ BHK3] |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office. PO box 844
Ames .IA 50010 USA
*
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
*
Vial, A1 from Freezer A was given to Elizabeth Morecroft (Pirbright)
26th April 2013 for sequencing.
Corresponding vial in freezer B was
not removed
*
[S1:
GQ506536,
S2: GQ506537,
S3: GQ506538,
S4: GQ506539,
S5: GQ506540,
S6: GQ506541,
S7:
GQ506542,
S8: GQ506543,
S9:
GQ506544,
S10: GQ506545]
Blood
originally taken for testing to clear animals for export to Brazil
|
