ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 14 (BTV-14)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection » Contents

 

 

 Family: ReoviridaeGenus: Orbivirus,  Species:   Bluetongue virus  serotype 14  (BTV-14)

Virus Species

 

(identification method)

Serotype

 

(identification method)

Place of original sample

eg country town

[grid reference] 

 

 (date original sample was taken)

Isolated from which host species

e.g cow,  sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

(Date added to collection)

IAH dsRNA virus collection number: [Country code year / sample number]

 

(Suppliers Isolate Designation)

Adapted to which cell types

 

Original sample material

 

(Passage history of sample)

* Original sample supplied to the collection by

person / institution

 

[IAH virus diagnosis, ISIS sample number]

Segments Sequenced

 

(sequencing in progress by person / institution)

Comments

References

&

 accession numbers

RSArrrr/14

BTV Type 14

Supplied by OVI

S. Africa*

 

(13/04/83)

NK

shelf C2, 
box C2γ,
rows A1-B10
 
Added to 
collection on 
4th Nov 2005

 

 

(Ref. Strain)

E1/BHK7

[IAH/BHK4]

 

*

Vial A1(g) supplied on 19th Dec 2007, to JongChul, JeongJa Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

Vial B1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial B2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology

29th Sept 2009

* 

Vial A sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

* 

[S2:AJ585135, S6:AJ586714]

USA2003/08

 

BTV-14

 

(serotyped

by Seg-2 

RT-PCR and sequence analyses)

Marion  County FL, USA

 

(sample taken

October 2003)

From sheep

 

clinical history: recumbent, anorexia, transient fever, lip swelling.

 

(isolate preparation date 25th Aug 2006)

Shelf E6, 
box E6 α,

rows G1 - H10

 

(added to collection on 15th June 2007)

04-279313-2

 

(tube labelled: 279313-2;

IVD 8/18, 19 B/1;

8-25-06)

From blood

 

E1/BHK2/E1/BHK1

[+IAH/ BHK4]

.

Virus isolate supplied by : USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

nvsluf@aphis.usda.gov

 

 

Derived from

USA2003/03

 repeated passage of original isolate generated virus of low titre, so the original isolate was inoculated into ECE in July / August 2006, followed by one pass in BHK-21 cells. original typing attempts as one of previous US types were unsuccessful

Technical contact in USA: Donna Johnson Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844 Ames IA, 50010 USA.[S2: ]

USA2003/03

BTV-14

 

(serotyped

by Seg-2 

RT-PCR and sequence analyses) 

Marion  County FL, USA

 

(sample taken

October 2003)

From sheep

 

clinical history: recumbent, anorexia, transient fever, lip swelling.

 

(isolate preparation date 25th Aug 2006)

Shelf D6, 
box D6 ALPHA,

rows A1 - B10

 

(added to collection on 11th May 2007)

04-279313-2

 

(tube labelled: 279313-2;

IVD 8/18, 19 B/1;

8-25-06)

From blood

 

E1/BHK2/E1/BHK1

[+IAH/ BHK3]

virus isolate supplied by :

 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

nvsluf@aphis.usda.gov

 

Used to generate

USA2003/08

[S2: ]

 

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

repeated passage of original isolate generated virus of low titre, so the original isolate was inoculated into ECE in July / August 2006, followed by one pass in BHK-21 cells. original typing attempts as one of previous US types were unsuccessful. 

BZE1990/02

Identified as BTV-14 by SNT in 1990

 

However, type specific RT-PCR   in  March 2008 failed to confirm serotype 14

Belize:

Orange Walk

 

Sample taken 11th Sept  1990

Water buffalo blood

Isolated in 1990

Shelf A15,

box A15 α,

rows I1-J10

 

(Added to collection

16th Oct 2008)

 

Virus ID: 90-46047-27

 

Tube labelled:

90-46047-27; IVD BHK/3-CPAE/1-BHK2;

3-12-08

E1/BHK3

 

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

Reagent also available from:

USDA, Aphis, VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA

 

nvsluf@aphis.usda.gov

CAR1982/04

BTV Type 14

Cameroon

(14/01/82)

 

NK

 shelf C2,

box C2β,

rows C1-D10 

 

Added to collection on 25th Oct 2005

83/1; 81/36

E2/BHK1

[IAH/BHK2]

 

  

 

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[S2:

S6:AJ586715]

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