ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 15 (BTV-15)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection Contents

 

 

 Family: ReoviridaeGenus: Orbivirus,  Species: Bluetongue virus,  serotype 15 (BTV-15) 

 *IAH dsRNA virus collection number

 

( ICTVdb isolate

Accession Number)

Virus Species

&

Serotype

 

 (identification method)

Place of original sample eg country town

 [grid reference]

 

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 

 Adapted to which cell types 

 

(Passage history of sample)

 

[IAH passage history]

* Original sample supplied to the collection by person / institution

 

[IAH, ISIS sample number]

 

Comments

 References &  accession numbers  

RSArrrr/15

 

 

(41010FC9)

BTV

Type 15

Supplied by OVI

S. Africa*

 (24/04/83)

NK   

Shelf B5,

Box B5β,

Rows C1 - D10

 

1st Aug 2005

(Ref. Strain)

E1/LK6/BHK5  

 

[IAH/BHK1]     

*

Two vials C1(g) supplied on 19th Dec 2007, to JongChul, Jeong, Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

Vial D1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial D2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

*  

Vial C sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

* 

 

 [Seg-1: KP820864,

 Seg-2: KP820984,

  Seg-3: KP821106,

  Seg-4: KP821226,

 Seg-5: KP821346,

  Seg-6: KP821466,

  Seg-7: KP821588,

  Seg-8: KP821708,

  Seg-9: KP821828,

  Seg-10: KP821949,

Nomikou et al 2015 (1)]

 

[S2:AJ585136,

  S6:AJ586716

Maan et al 2007]

 

ISR2010/22

BTV-15 *

 

Identified as BTV-15 by Seg-2 specific

 Real time

RT-PCR

Israel

Kfar Glikson

October 2010
Isolated from cattle blood at IAH-P

** Shelf C14,

box  C14β,

rows A1-B9

 

 Added to collection

5th July 2011

2714/10 
 
KVI Id 85818
 [KC1 at IAH-P]

Supplied by Jacob Brenner, Kimron Institute Israel, 

Samples arrived

6th April 2011

 

IAH Stars number

A32/11 21

 

*

Note: original blood sample was positive for BTV-8 and 15

*
Vials B10 were not added to freezer A & B
*

Vial A1 was removed from freezer A

*

Vial position  B8   was  taken out of Freezer   B  (only) and  given to NVRL  14/03/2016

*

 

ISR2010/21

BTV-15

 

 Identified as BTV-15 by Seg-2 specific

 Real time

RT-PCR 

Israel

Mizra

 

November 2010

Isolated from cattle blood at IAH

Shelf C14,

box  C14 α,

rows I1-J10

 

 Added to collection

4th July 2011

3138/10/04 
KVI Id 90830
 [KC1 at IAH-P]

Supplied by Jacob Brenner, Kimron Institute Israel

  

Samples arrived

6th April 2011

 

IAH Stars No.
A32/11 19

*

Note vial J10was not added to freezer A 

*
Vial position  J1  (P) from freezer A  and B removed on 29th Mar 2012
 

*
Vial position I1 was removed from freezer A
*
 
ISR2006/11

BTV-15

 

Identified as BTV by Seg-7 specific

 RT-PCR 

 

Identified as serotype 15 by Seg-2 specific RT-PCR

Israel,

 from a village called:  Yachini

 

samples

17th Nov 2006

Isolated in Israel from Sheep showing clinical signs

Shelf D5,

box D5α,

rows G1-H10

(Added to the collection

2nd Feb 2007)

 

 

isolate number

532452

 

(2302)

 

Supplied by Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15th Dec 2006

 

 

IAH Stars
number
 R12/06 -2

CE1

[IAH/BHK1]

*

Original sample held in Misc box

*

Duplicate vials  (position H10g) sent to Stephan Zientara, Affsa  Maisons Alfort, France 2nd July 2007

*

Duplicate vials position  G1p, taken out of freezers A and B on 6th Sept 2010

*

Vial position  G2 (p)  freezer A  and B given to Kyriaki Nomikou 24-Apr-2012 to prepare  RNA to be sent to Sanger Centre for sequencing.

Vial position  H2  (G) taken out of freezer A   and B 29th Mar 2012 

*

Vial, G10 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th April 2013 for sequencing.
*

Outbreak Data 

ZIM2003/09 

BTV

Type 15

 (typed by RT-PCR and sequence analysis of segment 2)

 Zimbabwe

(Tural Stud)

2003

 Culicoides magnus

Shelf A4,

Box A4γ,

Rows A1-B10

 (25/08/03)

[Stars number:

R06/03, No.151]    

(E1/BHK2)  

 

 [S2:           ]

[S6:AJ586718]

 ZIM2003/08

BTV

Type 15

 

 (typed by RT-PCR and sequence analysis of segment 2)

 Zimbabwe (Tural Stud) 2003

 Culicoides imicola

Shelf A4,

Box A4β,

Positions

E1-F10

 

 (Added to

collection on

25/08/03)

 [Stars number:

R06/03, No150]   

(E1/BHK2)

 

*

Vial, E1 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th April 2013 for sequencing.
*

 Vials F10 were removed from freezers a & b.

*

[S2:            

S6:AJ586717

AUS1988/03

BTV-15

Australia

(1988)

 

Shelf C18,

box C18β,

rows  I1-J10,

 

(Added to

collection on

18th June 2012)

Old isolate No.: D872 

 

Origin: DPP192

 

Sample sent as 'BTV-15'

Supplied by Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia.

 

Sent: April 2012

Note  supplied as   sample: CPE positive / BT-PCR positive.

 

It is uncertain if the date given is sampling date or date of arrival at EMAI.

 

??/2BHK21/

1HmLu-1 (PP)

[IAH/BHK1]

 

 (see also AUS1982/01)

 

ex BARC

*

Vial, I1 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th April 2013 for sequencing. Corresponding vial in freezer B  was  not removed
*

(MTA: not to be

supplied to 3rd 
parties without written 
consent from 
original source)
 
 

AUS1982/01

BTV Type 15

Australia, Northern Territories

(1986)

(G. Gard)

 

(DPP0192,

8409-11-1625)

BHK5

supplied by Dr. Ian Pritchard AAHLGeelong, Australia

 

*MTA* , not to be 
supplied to 3rd 
parties without written 
consent from 
original source
 
see also AUS1988/03

 

 
 
 

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