ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 19 (BTV-19)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection Contents

 

 Family: ReoviridaeGenus: Orbivirus,  Species:   Bluetongue virus (BTV) serotype 19 (BTV-19)

Virus Species

 

(identification method)

Serotype

 

(identification method)

Place of original sample

eg country town

 

[grid reference]

(date original sample was taken)

Isolated from which host species e.g cow,  sheep, etc

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

(Date added to collection)

IAH dsRNA virus collection number: [Country code year / sample number]

(Suppliers Isolate Designation)

Adapted to which cell types

Original sample material

(Passage history of sample)

* Original sample supplied to the collection by

person / institution

[IAH, ISIS sample number]

Comments

References

&

 accession numbers

RSArrrr/19

BTV-19
 
(western topotype,
reference strain) 

Supplied by OVI

S. Africa*

 

(13/06/83)

NK

 

Shelf B6, 
Box B6α
Rows G1-H10
 
(23 Aug 2005)
 

 

(Ref. Strain)

E3/BHK6

[IAH/BHK1]

 

*

Duplicate samples G1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

*

Vial H1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial H2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

Vial G sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

*  

[Seg-2: AJ585240,

Seg-6: AJ586722,

Maan et al 2004 (1),

 Singh et al 2004 (3),

 Maan et al 2007 (2)]

 

contents

 

USA2003/04

BTV-19

 

(serotyped

by Seg-2 

RT-PCR and Seg-

sequence comparisons)

 

Manatee  County FL, USA

 

(sample taken

21st October 2003)

From sheep

 

no clinical history given.

 

(isolate preparation date 12th Nov 2003)

Shelf E1,

 Box E1 α,

rows C1 to D10

 

(added to collection on 15th June 2007)

04-280559-3

 

(tube labelled: 280559-3;

IVD BHK/1;

11-12-03)

From blood

 

E1/BHK1

[IAH/BHK4 ]

virus isolate supplied by :

 

USDA, Aphis, VS, NVSL, PAS  Reagents Office.

PO box 844, Ames .IA 50010, USA

nvsluf@aphis.usda.gov

 

Technical contact in USA: Donna Johnson, Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844 Ames IA, 50010 USA. 

 

original typing attempts as one of previous US types were unsuccessful.  Multiple  other serotypes were isolated from this herd (BTV-2, -3, -10 and two isolates of unknown serotype:  see USA2003/05

[Seg-2:]

 

DOM1996/04

Identified as BTV-19

by SNT in 1996

 

Confirmed by type

 specific RT-PCR  and subsequent sequence analyses in 2008

Dominican Republic

 

Sample taken 22nd Jan 1996

Brahman cattle blood

Isolated in 1996

Shelf A15,

box A15 α,

rows E1-F10

 

(Added to collection 

 16th Oct 2008)

Virus ID: 96-13303-64

 

Tube labelled:

96-13303-64; IVD BHK/2;

3-12-08

E1/BHK2

 

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

Reagent also available from:

USDA, Aphis, VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA

 

nvsluf@aphis.usda.gov

 

DOM1996/02

original assays by SNT

 indicated BTV-2

 

however type specific

Rt-PCR targeting  Seg-2,

using multiple primer

 sets  subsequently

indicated type 19

Dominican Republic

 

Sample taken 22nd Jan 1996

Brahman cattle blood

Isolated in 1996

Shelf A15,

box A15 α,

rows C1-D10

 

(Added to collection 

 16th Oct 2008)

Virus ID: 96-13303-72

 

Tube labelled:

96-13303-72; IVD BHK/2;

3-12-08

E1/BHK2

 

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

Reagent also available from:

USDA, Aphis, VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA 

nvsluf@aphis.usda.gov

 

Vial position  C1 (P) from freezer A  and B  given to Kyriaki Nomikou 29-Mar-2012 to prepare  RNA to be sent to Sanger  Centre for sequencing
 
TAT1990/05

Initial attempts to identify

serotype by SNT were

 unsuccessful  in 1990

 

Type specific RT-PCR  and sequencing (Seg-2) 

March 2008

 identified type 19.  [1]

Trinidad:

St. Andrews

 

Sample taken 4th Sept  1990

Water buffalo blood

 

Isolated in 1990

Shelf A15,

box A15 γ,

rows C1-D10

 

(Added to collection 

 16th Oct 2008)

Virus ID: 90-44159-16

 

Tube labelled:

90-44159-16; YSD BHK/3 -

3-12-08

 

(sample prepared

12th Mar 2008)

E1/BHK4

 

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

Reagent also available from:

USDA, Aphis, VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA

 

nvsluf@aphis.usda.gov

 

 

 

References
1.

Maan S, Maan NS, Samuel AR, O'Hara R, Meyer AJ, Rao S, Mertens PP. (2004) Completion of the sequence analysis and comparisons of genome segment 2 (encoding outer capsid protein VP2) from representative isolates of the 24 bluetongue virus serotypes. Vet Ital. 2004 Oct-Dec;40(4):484-8.      http://www.ncbi.nlm.nih.gov/pubmed/20422574

 

2.

Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC. (2007) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes. Journal of General Virology  88, 621-630.

3.


Singh KP, Maan S, Samuel AR, Rao S, Meyer AJ, Mertens PP. (2004) Phylogenetic analysis of bluetongue virus genome segment 6 (encoding VP5) from different serotypes. Vet Ital. 2004 Oct-Dec;40(4):479-83.       http://www.ncbi.nlm.nih.gov/pubmed/20422573

 

 

 

 

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