ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 2 (BTV-2)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection » Contents

 

 

 

 

  Family: Reoviridae, Genus: Orbivirus, Species:  Bluetongue virus (BTV) serotype 2 

 *IAH dsRNA virus collection number

 

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 

 (identification method)

Place of original sample eg country town

 [grid reference]

 

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 

 Adapted to which cell types 

 

(Passage history of sample)

 

[IAH passage history]

* Original sample supplied to the collection by person / institution

 

[IAH, ISIS sample number]

Comments

 References &  accession numbers  

Reference and vaccine strains

 RSArrrr/02 

 

 

 

(41010DEC) 

BTV-2

 

(western topotype

-

reference strain)

Supplied by OVI S. Africa  to IAH Pirbright

8th Apr 1982

NK 

shelf B2, 
box B2γ,
rows C1-D10
&
shelf C2, 
box C2γ,
rows C1-D10
 
Added to 
collection on 
4th Nov 2005 

Ref. Strain

 

Strain:22/59H

BHK cells

 

(E2/BHK3) 

[IAH/BHK4]

 

Duplicate samples C1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon Dong,JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

* 

Duplicate samples, box C2γ-  position D2(p)

supplied to Oscar R. González Llamazares

 (Avenida Párroco Pablo Díez 49-57, 24010 - Leon - Spain,  22nd Nov 2007)

* 

Duplicate samples (Position C2g) Given to S. Maan (IAH Pirbright)  12th Aug 2008

* 

Vial D2 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial C3 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

*

Vial C sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011 

*   

» contents

 

[Seg-1: KP820910,

Seg-2: KP821030,

Seg-3: KP821152,

Seg-4: KP821272,

Seg-5: KP821392,

Seg-6: KP821512,

Seg-7: KP821634,

Seg-8: KP821754,

Seg-9: KP821944,

Seg-10: KP821995

Nomikou et al 2015 (1)]

 

 [S2: AJ585123,

S3: DQ186793

S5: AM773705,  

S6:AJ586696

 

Maan et al 2007

  RSAvvv2/02

BTV

Type 2 

South Africa Onderstepoort Vaccine strain   

 

 Shelf A1

Box ,

Rows

 

(31/01/2003)

 

 

Dr. Annalisa Guercio  & Giuseppina Alimena) IZS Palermo 

tubes 1/10a(g)-

 &

1/10a(p)-D1 given to Helen Jones,  Arbovirology IAH Pirbright 07/06/2004

[S2: AJ585157

S5: AM773684]

 RSAvvvv/02

BTV

Type 2 

South Africa Vaccine (Onderstepoort)  

 

Shelf B4,

box B4β

Rows  E1-F10

BTV-2 vaccine  strain,

 

batch no.19 exp.01/12/2005 BL6093S

[IAH/BHK1]

 

Duplicate vials, position E1(g) supplied to Sushila Maan (IAH Pirbright) on 29th April 2009, for  sequencing » contents

   [S6:AJ586665 ]

2011
ISR2011/03

BTV-2

 Identified as BTV-2

Israel
 2011
 
Shelf C16, 
box C16 gamma,

rows I1 - J10

       

ISR2011/02

BTV-2 Identified as BTV-2 by Seg-2 specific

 Real time RT-PCR 

Israel
Afikim, 

August 2011

Cattle  Blood

 Isolated through in eggs at Kimron

Shelf C16, 
box C16 beta,

rows I1 - J10

 

Added

KVI Id 115249

Lab no: 1822

[IAH/KC1]

Jacob Brenner

Kimron Institute Israel

 

 

Samples arrived 3rd November 2011

 

IAH Stars
number

A70/11 02

Vials I10 removed from freezer A & B, vial J10 was removed from freezer A.
2010
ISR2010/34 BTV-2 collected
in 2010
  Shelf C17,
box  C17 Beta, 
rows G1-H10
       
ISR2010/33 BTV-2 collected
in 2010
  Shelf C17,
box  C17 Beta, rows E1-F10
       
ISR2010/32 BTV-2 collected
in 2010
Shelf C17,
 box  C17 Alpha, rows I1-J10
ISR2010/31 BTV-2 collected
in 2010
Shelf C16,
box  C16 Gamma, rows G1-H10
ISR2010/30 BTV-2

Identified as BTV-2 by Seg-2 specific

 Real time RT-PCR 

Israel,
Elishema

28th
November 2010
Isolated from cattle blood
at
TPI

Shelf C15,

box  C15 Gamma,
rows A1-B10
 

 
Added 4/10/11

3121

KVI Id 90616
[KC1 at IAH-P]

Jacob Brenne, Kimron Institute Israel  

Samples arrived 30th August 2011

 

IAH Stars number
 A59/11 05
ISR2010/29 BTV-2

Identified as BTV-2 by Seg-2 specific

 Real time RT-PCR 

Israel

Zahal

30th November 2010
Isolated from
Eland spleen, at TPI

Shelf C15,

box  C15 β,
rows I1-J10

Added 4/10/11 

3134

KVI Id 90888
 [KC1 at IAH-P]

Jacob Brenner

Kimron Institute Israel Samples arrived 30th August 2011

 IAH Stars number

 A59/11 04
 
ISR2010/28 BTV-2

Identified as BTV-2 by Seg-2 specific

 Real time RT-PCR 

Israel
Bet Gamliel

20th
September 2010
Isolated from cattle
 blood at IAH-P

Shelf C15,

box  C15 β,
rows G1-H10

Added 4/10/11

2208

KVI Id 84336
 [KC1 at IAH-P]

Jacob Brenner

Kimron Institute Israel

Samples arrived 30th

August 2011

 

 
ISR2010/24

* BTV-2

 

Identified as BTV-2 by Seg-2 specific

 Real time

RT-PCR 

Israel Isolated from cattle blood at IAH-P

Shelf C14,

box  C14 β,
rows E1-F10

 Added  to collection
5th July 2011

Calf purchased for KVI

870 KVI Id

 [IAH/KC1]

** Jacob Brenner

Kimron Institute Israel  

Samples arrived 6TH April 2011

 

IAH Stars No.
A32/11 6

* Note: Originally blood was typed as BTV-8

**Jacob confirmed BTV-2 was in the area in 2004, many samples could be from mixed infections

Vials F1 were removed from freeezers A & B

2008
AUS2008/01 BTV-2 Australia   Shelf D12,
box D12 α,
rows I1 to J10,

tube labelled:

BTV-2 - #0912-17-0002
[IAH/BHK1] AAHL (BT/2010/145)   Distribution restricted by MTA
2006
USA2006/04

BTV-2

 

(typed by

Seg-2 RT-PCR and sequencing)

Hillsborough  County, FL,

USA

 

(sample taken

13th Dec 2006)

From sheep

 

(clinical history: oral lesions, cough, respiratory congestion)

 

Date of virus

 preparation in USA

3rd Jan 2007

Shelf E3,

box E3α,

rows G1 – H10

 

Added to collection on

17th Sept 2007 

 

06-473635

 

(tube labelled: 473635;

 IVD BHK/2;

1-3-07)

From blood

 

E1/BHK2

[IAH/ BHK4]

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

2005
MAR2005/01 BTV-2 Martinique (France)

(Sample taken Dec  2005)
from a
non-vaccinated bovine

Shelf C7,

box C7 α ,

rows C1-D10

 

(Added to collection

9th  Aug 2010)

tube labelled:

BTV-2/06.01 - 108
[IAH/BHK1] Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France

23rd July 2010
  Distribution restricted by MTA

[S2:]

SPA2005/01

Identified as BTV by RT-PCR  and

typed as BTV-2 by Seg-2 type specific RT-PCR and sequence analysis

Spain

 

blood sample

taken on

11th Jul 2005

Virus isolated from cattle blood sample at IAH Pirbright by Sue Graham

Shelf C3, 
box C3γ.
rows A1-B10

 

(added to 
collection 
25th Nov 2005)

blood sample number

4077-23

[IAH/E1/BHK2]

Miguel Angel Jiménez-Clavero

 

Departamento de Enfermedades  Emergentes

 

[R10/05]

*

Sample C3 from both freezers given to K. Nomikou (IAH) , for sequencing 7th July 2011

* 

[S2: AM773697, S5: AM773685]

 

 OIE report -14th Oct 2005

2003

USA2003/08

BTV-2

 

Identified as BTV-2 by

RT-PCR and sequence analysis of

Seg-2

Mantee County Florida

USA

 

Sample taken 21st Oct 2003

Virus isolated from Cattle blood

 

12th Nov 2003

Shelf A16

Box A16 gamma,

Rows H1-I10

 

(added to collection on 15th June 2007)

04-280559-6

 

tube No. 280559-6;

IVD BHK/1;

11-12-03

from blood

 

ECE1/BHK1

[IAH/KC2]

 

Related to isolate USA2003/01

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

 [S2: ]

 

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

Note multiple serotypes of BTV were isolated from the same herd: BTv-2, 3, 10 and isolates of unknown serotype.

USA2003/01

BTV-2

 

Identified as BTV-2 by

RT-PCR and sequence analysis of

Seg-2

Mantee County Florida

USA

 

Sample taken 21st Oct 2003

Virus isolated from Cattle blood

 

12th Nov 2003

Shelf E1, 
box E1β,

rows A1 - B10

 

(added to collection on 15th June 2007)

04-280559-6

 

tube No. 280559-6;

IVD BHK/1;

11-12-03

from blood

 

ECE1/BHK1

[IAH/BHK3]

 

Related to USA2003/08

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

 [S2: ]

 

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

Note multiple serotypes of BTV were isolated from the same herd: BTv-2, 3, 10 and isolates of unknown serotype.

IND2003/03

BTV

Type 2

 

 (Typed by SNT & by Seg-6 RT-PCR / sequence analysis)

Tiruneveli,

Tamil Nadu, India

Sheep

 

(Isolated at Tamil Nadu Veterinary And Animal Sciences University, Chennai-600 051 (India))

Shelf A6

Box A6β

Rows E1-F10

(138)

BHK cells

 

(BHK5)

[IAH/E1/BHK3]

Dr A. Wilson Aruni

Tube F1 (freezer A&B) given to Kiki Nomikou,  Athens Vet lab, Greece,  Oct 2004

*

Tube F2 given to Sushila Maan IAH Pirbright  04th May 2006

*

Tube E10 removed from freezer A&B

*

[Seg6:AJ783906]

(MTA)

IND2003/02

BTV

Type 2

 

 (Typed by SNT & by Seg-6 RT-PCR / sequence analysis)

 

Tiruneveli,

Tamil Nadu, India

Sheep

 

(Isolated at Tamil Nadu Veterinary And Animal Sciences University, Chennai-600 051 (India))

Shelf B6, 
Box B6α
Rows E1-F10
 
(added to 
collection on 
23 Aug 2005)

(132)

BHK cells

 

(BHK5)

[IAH/E1/BHK2]

Dr A. Wilson Aruni

sample 1/10g given to Sushila Maan IAH Pirbright 

04th May 2006

[Seg6:AJ783905]

(MTA)

IND2003/01

BTV

Type 2

 

 (Typed by SNT & by Seg-6 RT-PCR / sequence analysis)

 

Tiruneveli,

Tamil Nadu, India

Sheep

 

(Isolated at Tamil Nadu Veterinary And Animal Sciences University, Chennai-600 051 (India))

Shelf B5,

box B5γ,

rows G1- H10

 

(added to collection on 16/08/2005)

 

(102)

BHK cells

 

(BHK5)

[E1/BHK3]

Dr A. Wilson Aruni

 

Sample 1/10g given to Sushila Maan IAH Pirbright 

04th May 2006

-----------------

Duplicate samples

G1(g) supplied on 19th Dec 2007, to JongChul, Jeong

Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

[Seg6:AJ783904]

 

(MTA)

 ZIM2003/01

 

BTV

Type 2

 

 (Typed by

Rt-PCR)

Zimbabwe

 (Lone Oak)

 

 (25/08/03 )

Cuilcoides imicola 

Shelf A4,

Box A4β,

Positions

G1-H10

 

BHK cells

 

(E1/BHK2)

[R6/03 No. 92]   

Tube G10  freezer A and B given to Sushila Maan IAH Pirbright  04th May 2006

*

Vial position  H10 was removed from  freezer A  and B 29th Mar 2012,

Vial position G9 from Freezer A given to Elizabeth Morecroft (Pirbright) 9th Jul 2013. 

Tube H9 was removed from the collection

*

[S2:     ]

2002

 

 ITL2002/07

BTV

Type 2

 

 (Typed by SNT & by Seg-2 RT-PCR / sequence analysis)

Sicily  S.Angelo di Brolo (ME)

 Farm code:  088ME036 E 14o 54432 N 38o 04925 Alt. 650 (18/Jul/2002)

From Sheep (spleen)

(isolated at: IZS Palermo by: Dr. Annalisa Guercio & Giuseppina Alimena)

Shelf A1,

Box A1γ, Positions

E1-F10

 

 (31/Jan/2003)

(32990/A010)  

 BHK cells

 

 (E1, BHK3)

[IAH-BHK4]

 

Dr. Annalisa Guercio  & Giuseppina Alimena) IZS Palermo

 

Isolated from the same location (same farm) as ITL2002/05

 

Tubes E1 & F1  from freezer A supplied to  S. Anthony & H. Jones, Pirbright  Institute 7th June 2004

Vial position E2  Freezer A given to C. Batten (IAH Pirbright) 21 Sept 2008 for ring trial

spare vial from freezer B  held in misc box

 

*

Sample E3 from freezer A given to K. Nomikou (IAH) , for sequencing 7th July 2011

* 

[S2:AJ585160, S3:DQ186815,

S5: AM773688 S6:AJ586671]

 ITL2002/06    

BTV

Type 2

 

(Typed by SNT & by Seg-2 RT-PCR + sequence analysis)

Sicily

 Raccuja (ME) Farm code: 069ME006 E 14o 55664  N 38o 01318 Alt. 493

 

 (06/Aug/2002)

From a pool of 348   Culicoides pulicaris

 

(isolated at: IZS Palermo by: Dr. Annalisa Guercio & Giuseppina Alimena) 

Shelf A1,

Box A1β, Positions

 E1-F10

 

 (31/Jan/2003)

(36183)

BHK cells

 

 

 (E1, BHK3)

[IAH-BHK1]

Dr. Annalisa Guercio  & Giuseppina Alimena) IZS Palermo

 

Note: TL2002/ 01  to  ITL2002/06

Represent some of the first

field isolates from C. Pulicaris

 

Tubes E1 &  F1 from freezer A

 supplied to  S. Anthony

 & H. Jones, Pirbright Institute

7th June 2004

 

 

[S2: S6:AJ586670,  S10:      ]

 ITL2002/05    

BTV  

 Type 2

 

 (Typed by SNT & by Seg-2 RT-PCR + sequence analysis)

Sicily  S.Angelo di Brolo (ME) Farm code:  088ME036 E 14o 54432 N 38o 04925 Alt. 650

 (06/Aug/2002)

from a pool of 122 Culicoides pulicaris (At: IZS Palermo  by: Dr. Annalisa Guercio & Giuseppina Alimena)

Shelf A1,

Box A1β, positions

C1-D10

 

 (31/Jan/2003)

(36179)

BHK cells

 

 (E1, BHK3)

[IAH-BHK1]

 

Dr. Annalisa Guercio  & Giuseppina Alimena) IZS Palermo Isolates

 Isolated from the same location (same farm) as ITL02/07

Tubes C1+2 & D1+2 from freezer A  supplied to  S. Anthony &

 H. Jones,  Pirbright Institute

7th June 2004

 

D3 from freezer A also removed from collection

 

 » contents 

 

 [S6:AJ586669 ]

 ITL2002/04    

BTV

 Type 2

 (Typed by SNT & confirmed by Seg 2 RT-PCR)

Sicily  S. Pietro Patti (ME) Farm code: 081ME039 E 14o 975933 N 38o 0079 Alt.1080

(06/Aug/ 2002)

from a pool of 156 Culicoides pulicaris

(isolated at: IZS Palermo   by: Dr. Annalisa Guercio & Giuseppina Alimena)

 Shelf A1,

Box A1β,

positions

 A1-B10

 (31/Jan/2003)

(36178)

BHK cells

 (E1, BHK3)

[IAH-BHK1]

Dr. Annalisa Guercio  &

Giuseppina Alimena

IZS Palermo, Sicily

 

Note: TL2002/ 01  to  ITL2002/06

Represent some of the first

field isolates from C. Pulicaris

Tubes A1 & B1 from freezer A

 supplied to S. Anthony  &

H. Jones,  Pirbright Institute

7th June 2004

 

    [S10:         ]

ITL2002/03

BTV

Type 2

 

 (Typed by SNT and confirmed by Seg 2 RT-PCR) 

Sicily

 Novara Di Sicila (ME)

Farm code: 062ME005 E 15o 06352 N38o 00130 Alt. 491

 

 (24 July 2002)

 from a pool of 200 Culicoides pulicaris

(Isolated at: IZS Palermo   by: Dr. Annalisa Guercio & Giuseppina Alimena)

Shelf A1,

Box A1α,

Positions

I1-J10

 (31/Jan/2003)

(34125)

BHK cells

 

(E1, BHK3)

[IAH-BHK1]

 

Dr. Annalisa Guercio  &

Giuseppina Alimena

IZS Palermo, Sicily

Tubes I1 & J1 from freezer A

 supplied to S. Anthony  &

H. Jones, Pirbright Institute 7th June 2004

*

 

 » contents

 

 [S10:         ] 

ITL2002/02

BTV

Type 2

 

(Typed by SNT,    Type confirmed by Seg 2 RT-PCR)  

Sicily 

Novara Di Sicila (ME)

Farm code 062ME015 Grid reference E 15o 07089 N 38o 00421 Alt 575

 

 (18th July 2002)

 from a pool of 18 Culicoides pulicaris

 

(Isolated At: IZS Palermo    by: Dr. Annalisa Guercio & Giuseppina Alimena)

Shelf A1,

Box A1α,

Positions

G1-H10

 

 (31/Jan/2003)

(33669)

   BHK cells

 

(E1, BHK5)

Dr. Annalisa Guercio  &

Giuseppina Alimena

IZS Palermo, Sicily

* 

Tubes G1and H1 from freezer A supplied to 

S. Anthony and H. Jones, 

 Pirbright Institute

7th June 2004

*

ITL2002/01

BTV

Type 2

 

(Typed by SNT &     

confirmed by Seg 2

RT-PCR)

Sicily 

 

Novara Di Sicila (ME) Farm code 062ME015 Grid reference E 15o 07089 N 38o 00421 Alt 575

 

 (18/ Jul/2002)

from a pool of 18 Culicoides pulicaris

 

(Isolated at: IZS Palermo    by: Dr. Annalisa Guercio & Giuseppina Alimena)

Shelf A1,  

Box A1α, Positions

E1-F10

 

 (31/Jan/2003)

(33669)

   BHK cells

 

 (E1, BHK3)

[IAH-BHK1]

Dr. Annalisa Guercio  &

Giuseppina Alimena

IZS Palermo, Sicily

Tubes A1+2 &

F1+2 from freezer A supplied to

S. Anthony and H. Jones,  IAH Pirbright  

7th July 2004

 

 » contents

 

[S2:AM773708

S5:AM773687]

SAD2002/03

BTV

Type 2

Sardinia (02/01/02)

Sheep

Shelf A6,

Box A6γ

Rows C1-D10

 (B4)

BHK cells

 

(E1/BHK2)

[IAH-BHK1]

 

aliquots D1 (freezer A&B) Sent to Kiki Nomikou, Athens Vet Labs ,  Greece, Oct 2004

 

[S10: ]

SAD2002/02

BTV

Type 2

Sardinia

 

Isolated at IAH from Sheep blood collected on

2nd January 2002)

Sheep

Shelf D5

box D5α,

rows I1-J10,

 

Added to the collection on the 2nd Feb 2007

 (13 P)

BHK cells

 

(IAH/E1/BHK2)

 

*

Vial positions  E1 (p)  were given to Eva Veronesi (IAH Pirbright on 13th May 2010 for Ph.D studies).  one of these was retained in Misc box C,  bottom of freezer A
position E5

*

Duplicate samples E3 given to K. Nomikou (IAH)  for sequencing 7th July 2011

* 

SAD2002/01

BTV

Type 2

Sardinia (02/01/02)

Sheep

Shelf  D1,
  Box D1ß,
Rows G1 to H 12
 
(Added to 
collection on 
8th Aug 2006)

(10 P)

Adapted to BHK cells

 

E1/BHK2

+

 [IAH/BHK1]

 

[S10: ]
*
Duplicate sample from position 
position A1p sent to John Murray / Jeremy Salt 
Moredun Scientific Scotland  6th Sept 2010.  
*
2001

ISR2001/17

 

 

 

 

BTV

Type 2

 

yped by

 Seg-2 RT-PCR and sequence comparisons

Israel, Yokneam

Sheep (27/Feb/2001 at the Kimron Institute)

Shelf B6, 
Box B6β
Rows I1-J10
 
(Added to
collection on
1st Oct 2005)

(300180

Lab No:823/2)

 

(E1)

[IAH/BHK5]

 Dr. Hagai Yadin,

 Kimron Institute Israel

(MTA)

» contents

FRA2001/07

BTV

Type 2

Corsica
2nd Sept 2001

Sheep

Isolated at TPI from spleen

Shelf D12,

box D12 alpha,

rows G1-H10

  

(Added to

collection on

23rd July 2014)

[R16/ 01 - 21]

BHK cells [E1/BHK4]

Supplied by Stephan Zientara, AFSSA, Maisons Alfort France September 2001

 

FRA2001/06

BTV

Type 2

Corsica

 

2nd Sept 2001

Sheep

Shelf A1,

 Box A1γ,

Rows G1-H10     

[R16/ 23]

BHK cells

(E1/BHK2)

*

 Tubes G1 & H1 Freezer A  supplied to  S. Anthony  & H. Jones,   IAH Pirbright  07-06-2004 

*

  Tubes position G2  Freezer A given to C. Batten (IAH-P)  28th April 2010 for  Ring Trial

  * 

 » contents

FRA2001/05

BTV

Type 2

Corsica

 

2nd Sept 2001

 

Sheep

Isolated at TPI from spleen

 

Shelf D12,

box D12β, rows

E1-F10

  (Added to

collection on

23rd July 2014)

[R16/ 14]

BHK cells

 

 

 [E1/BHK3]

Supplied by Stephan Zientara, AFSSA, Maisons Alfort France September 2001

 

[S10: ]

FRA2001/04

BTV

Type 2     

Corsica (12/09/01)

Sheep

Shelf B5,

Box B5β,

Rows I1 - J10

 

 (added to collection on

1st Aug 2005)

 

[R16/ 12]

BHK cells

 

(E1/BHK2)

 

[IAH/BHK2]

 

 

 » contents

 

 

FRA2001/03

BTV

Type 2

 

(typing confirmed by Seg 2 RT-PCR and sequencing)

Corsica (12/09/01)

Sheep 

Shelf B1,

Box B1α

Rows C1-D10

 

[R16/ S2]

BHK cells

 

(BHK2)  

 

[IAH/BHK1] 

 

 

[S2: AJ585121, S5: AM773686,  S6:AJ586674]

*

Sample D2 from both freezers given to K. Nomikou (IAH) , for sequencing on 7th July 2011

*

FRA2001/02

BTV

Type 2

 

Corsica

 (12/09/01)

Sheep

Isolated at AFSSA- Maisons Alfort France

  September 2001 

Shelf D12,

Box D12β,

rows C1-D10

 

 (Added to

collection on

23rd July 2014)

[R16/ S1]

BHK cells

 

E1/BHK1[BHK3]

Supplied by Stephan Zientara, AFSSA, Maisons Alfort France September 2001 as BHK cell culture material

 

 » contents

 

 

FRA2001/01

BTV

Type 2

Corsica

 (31/07/01)

NK

shelf C5
 box C5α

rows E1-F10

(Added to collection on

22 Dec 2005)

[R10/01; BT44]

BHK cells

(V1/BHK3)

[IAH/BHK2]

 

[S10:             ]]

SAD2001/08

BTV

Type 2

From Sardinia

 

Isolated at IAH Pirbright

 

(Sample taken 29th Dec 2001)

Sheep

Shelf C15,

box C15γ,

rows I1-J10

 

Added to collection on
14 Oct 2011

(4P)

BHK cells

 

[IAH/E1/BHK1/KC1]

  Derived from SAD2001/01
*
100ml of this passage level sent to Michael Eschbaumer  at FLI Rheims Germany , as a vaccine challenge strain on 13Oct 2011 under MTA
*
100ml of this passage level sent to Piet van Rijn CVI at Lelystad the Netherlands, as a vaccine challenge strain under MTA
*

SAD2001/07

BTV

Type 2

Sardinia (29/12/01)

isolated from Sheep

Shelf C4, 
box C4α
rows A1-B10

 

(Added to collection on

21st Dec 2005)

(FF7)

Adapted to BHK cells

 

E1/BHK2

[IAH/BHK2]

 

 

[S10:    ]

SAD2001/06

BTV

Type 2

Sardinia (29/12/01)

Sheep

Shelf C4, 
box C4β
rows I1 - J10
 
(added to 
collection
 19th Dec 2005)

(FF4)

adapted to

BHK cells

 

E1/BHK2

[IAH/BHK2]

 

 

 » contents

 

[S10: ]

SAD2001/05

BTV

Type 2

Sardinia (31/12/01)

Sheep

Shelf C4, 
box C4β
rows G1 - H10
 
(added to 
collection
 19th Dec 2005)

(17 P)

adapted to

BHK cells

 

E1/BHK2

[IAH/BHK2]

 

 

 » contents

 

[S10: ]

SAD2001/04

BTV

Type 2

Sardinia (29/12/01)

Sheep

Shelf C4, 
box C4β
rows E1 - F10
 
(added to 
collection
 19th Dec 2005)

(12 P)

adapted to

BHK cells

 

E1/BHK2

[IAH/BHK2]

 

 

 » contents

 

[S10: ]

SAD2001/03

BTV

Type 2

Sardinia (29/12/01)

Sheep

Shelf A6,

Box A6γ

Rows E1-F10

 (7 P)

BHK cells

 

(E1/BHK2)

[IAH-BHK1]

 

aliquots F1 (freezer A&B) sent to Kiki Nomikou, Athens Vet Labs ,  Greece, Oct 2004

 

 » contents

 

[S10: ]

SAD2001/02

BTV

Type 2

Sardinia (29/12/01)

Sheep

Shelf C4, 
box C4β
rows C1 - D10
 
(added to 
collection
 19th Dec 2005)

(6P)

adapted to BHK cells

 

E1/BHK2

[IAH/BHK2]

[S2: AJ585162

S5:  AM773706 S6:AJ586673

SAD2001/01

BTV

Type 2

From Sardinia

 

Isolated at IAH Pirbright

 

(Sample taken 29th Dec 2001)

Sheep

Shelf D6,

box D6β,

rows A1-B10

 

Added to collection on 2nd Feb 2007

(4P)

BHK cells

 

[IAH/E1/BHK2]

 

 » contents

*

Duplicate vials position A1 (P), taken out of freezers A and B on 6th Sept 2010

*

Used to generate SAD2001/08 as vaccine challenge strain

*

Duplicate vials position A4 from freezers A and B given to K. Nomikou (3rd May 2012) to prepare

 SAD2001/08,

*

Vial position  B2 (G) taken out of freezer A  and B 29th Mar 2012  

[S6:AJ586672]

2000

TUN2000/02

BTV

Type 2   

Tunisia

NK

 

[R2/00; BT 74]

 

 

 

TUN2000/01

BTV

Type 2

Tunisia

(3rd Feb 2000)

NK

shelf B5,

box B5γ,

rows A1-B10

 

(added to collection on 16/08/2005)

[R1/00 ;  BT 74]

BHK cells

 

(E1/BHK2)

[IAH/BHK3]

 

 

 » contents

 

[Seg2:AJ585156

Seg3:DQ186826

Seg5:AM773689

 Seg6:AJ586668

 

1994
IND1994/01

BTV-2

South India 

Shelf C16,

box C16α,

rows E1-F10


Added to collection
2006

Unknown passage

level in BHK cells

[IAH/ BHK2]

 

Sample given to Sushila Maan (IAH Pirbright) Sept 2006 for sequencing studies

 (MTA)

1993
IND1993/01 BTV-2

Typed by real-time RT-PCR targeting Seg-2
Southern India

(1993)
From sheep
Shelf C16, 
box C16α
rows A1-B10

 Added to collection  2006

Unknown passage level in BHK cells

 [IAH/BHK2]
Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies
*
(MTA)
1985

SUD1985/01

BTV

Type 2

Sudan (15/01/85)

NK

Shelf C4, 
box C4α
rows C1-D10

 

(Added to collection on

21st Dec 2005)

 (GP-4; SVD9/83)

adapted to BHK cells

 

LD3/BHK3

[IAH/BHK2]

 

 

[S2: S6:AJ586666]

1983
SUD1983/07
(SUD----/03)

BTV-2 

Sudan

Nyala

(western Sudan)

Isolated at IAH Pirbright, from a pool of Culicoides collected in Sept/Oct 1983

Shelf B11,

box  γ,

rows C1-D10

 

Added  to collection  
22nd Oct 2009
GP14

[LD3/BHK5/

PP2/BHK1**]

M.E.H. Mohammed    
SUD1983/05
(SUD----/01)

BTV-2

Sudan

Nyala

(western Sudan)

Isolated at IAH Pirbright, from a pool of Culicoides imicola collected in Sept/Oct 1983

Shelf B11,

box γ ,

rows A1-B10

 

Added  to collection  
22nd Oct 2009
GP4 [LD3/BHK5**] M.E.H. Mohammed Vial Position A2 was from freezer A was givemn to Kyriaki Nomikou (Pirbright) on 14th February 2013
Equivalent vial  in Freezer B was not removed
1982

IND1982/01

BTV

Type 2  

India

(26/03/82)

NK 

shelf C2,

box C2β,

rows G1-H10

 

Added to collection on

25th Oct 2005

(IND - 171)

BHK cells

 

(E2/BHK2 )

 

[IAH/BHK1]

 

Sample Nos. 1/10g(a+b)  were given to Natalie Ross-Smith (IAH Pirbright) for sequencing studies,

 7th March 2006

[S2: AJ585152, S6:AJ586675]

 

(MTA)

NIG1982/02

BTV

Type 2

 

(typing confirmed by Seg 2 RT-PCR and sequencing)

Nigeria

 (05/09/82)

NK

shelf C5
 box C5α

rows A1-B10

 

(Added to collection on

22nd Dec 2005)

( N –350)

BHK cells

 

(E1/BHK3)

[IAH/BHK2]

 

 

 » contents

 

[S2: S6:AJ586667]

1971
RSA1971/07

BTV-2

 

(see BTV2 RSA1971/03)

Grown at IAH in  sheep from a freeze dried blood sample of RSA1971/03. Originally sampled from the field  between

1968-1972

Sheep, D8 post infection, sheep clinically ill 26th April 2014  BTV2 RSA1971/03 Original sample:
Freeze dried blood from sheep infected with BTV-2 RSA1971/03
This sample is derived from  
RSA1971/03


 (IAH/5 x sheep and 2 x egg)
   

RSA1971/03

BTV-2

 

confirmed by serotype specific RT-PCR targeting genome segment 2

Grown at IAH in Dorset Poll sheep from a sample of virus  originally isolated between

1968-1972

 

 

(3032)

no passage in cell culture

This virus has been passed 4 times in sheep. It has been grown in Eggs and the last two sheep passes were infected with freeze dried blood plus infected egg material  

Freeze dried Virus in sheep blood

(Dorset Poll)

 

*IAH dsRNA virus collection number: [Country code, year / sample number] rrrr = reference strain vvvv = vaccine strain]

**  PP = Plaque purified (cloned)   -    LD = purified /'cloned' by limit dilution

 

References
1.     
 

Nomikou K, Hughes J, Wash R, Kellam P, Breard E, Zientara S, Palmarini M, Biek R, Mertens P. (2015) Widespread Reassortment Shapes the Evolution and Epidemiology of Bluetongue Virus following European Invasion. PLoS Pathog. 2015 Aug 7;11(8):e1005056. doi: 10.1371/journal.ppat.1005056. eCollection 2015.

   

 

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