ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

Back to dsRNA virus Homepage 

virus-nos-by-country ] BTV-Nos.htm ] Virus-nos ] BTV-1 ] BTV-2 ] BTV-3 ] BTV-4 ] BTV-5 ] [ BTV-6 ] BTV-7 ] BTV-8 ] BTV-9 ] BTV-10 ] BTV-11 ] BTV-12 ] BTV-13 ] BTV-14 ] BTV-15 ] BTV-16 ] BTV-17 ] BTV-18 ] BTV-19 ] BTV-20 ] BTV-21 ] BTV-22 ] BTV-23 ] BTV-24 ] BTV-25 ] BTV-26 ] Rescued-BTV ]

 

Isolates of Bluetongue virus type 6 (BTV-6)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection Contents

 

Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus (BTV) serotype 6 

Virus Species

 

* IAH dsRNA virus collection number

Serotype (identification method)

Place of original sample eg country town [grid reference] (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc (Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

(Date added to collection)

Suppliers Isolate Designation)

Adapted to which cell types Original sample material (Passage history of sample)

* Original sample supplied to the collection by person / institution [IAH virus diagnosis, ISIS sample number] 

 

Comments

 

 

 

References &  

[accession numbers]

  RSArrrr/06

BTV- 6

 

(western topotype

-

 reference strain)

Supplied by OVI S. Africa* (25/06/82)

 

Shelf B3, 
Box B3γ,
Rows A1 -B10
 
(Added to 
collection on 
20th May 2005)
(BTV-6 
Ref. Strain)
E3/BHK4
[IAH/BHK1]

*

Duplicate samples A1(g) supplied on 19th Dec 2007, to JongChul, Jeong, Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do,440-330, Korea

Vial B1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial B2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

* 

Vial A sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

*  

Vial position A5(g) was   taken out of Freezer A on 31st May 2013 and given to Marcela Gasparini (Pirbright) to make reference RNA for use in Brazil (Corresponding vial in freezer B  has  not been removed)

*

Vial, B4 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th April 2013 for sequencing. Corresponding vial in freezer B  was  not removed
*

 

 

 

 

[Seg-1:GQ506498

Seg-2:AJ585127

Seg-3: GQ506499,

Seg-4: GQ506500,

Seg-5: GQ506501,

Seg-6: AJ586703,

Seg-7: GQ506502,

Seg-8: GQ506503

Seg-9: GQ506504,

Seg-10:  GQ506505

Maan et al 2007 (3);

Maan et al 2010 (4)]

 

RSAvvvv/06 BTV-6 vaccine strain S. Africa     BTV-6 vaccine strain     [Seg-1: GQ506506,
Seg-2: GQ506507,
Seg-3: GQ506508,
Seg-4 GQ506509,
Seg-5: GQ506510, 
Seg-6: GQ506511,
Seg-7: GQ506512, 
Seg-8: GQ506513,
 Seg-9: GQ506514, 
Seg-10:  GQ506515

Maan et al (2010)]

GER2008/14

BTV-6

 

Identified by BTV-6 specific real time PCR LSI assay

Germany

 

(sample taken

2008)

 

blood from experimentally infected cattle

Shelf B18,

box B18 β ,

rows E1-F10

 

Added to collection

29th  July 2010

from

Animal No. 15

(12 dpi)

[IAH

KC1/BHK1]

Derived from
GER2008/12

From Bernd Hoffmann

[Stars no. A176/08 3]
Received at IAH  approximately  12th Dec 2008

Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman

GER2008/12

BTV-6

 

Identified by BTV-6 specific real time PCR LSI assay

Germany

blood from experimentally infected cattle

Shelf B18,

box B18 α ,

rows I 1-J 10

 

(Added to collection

28th  July 2010)

Animal No. 15

(12 dpi)

[IAH/KC1])

Derived from GER2008/05,

used to generate
GER2008/14

Bernd Hoffmann


[Stars no. A176/08 3]

Received at IAH  approximately  12th Dec 2008
Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman
GER2008/07

BTV-6

 

contains BTV-6 and BTV-8 by real-time and conventional RT-PCR,

 but only BTV-6 was isolated : see GER2008/02 

Germany

Received at IAH  approximately  12th Dec 2008

Positive field cattle blood

Shelf B7,

 box B7 α.

 rows I1-J10

 

(Added to collection
 16th Jan 2009)
Animal No. BH266/2 original blood sample

Bernd Hoffmann


A176/08 2
Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman

Used to generate
GER2008/02
GER2008/06

BTV-6

 

contains BTV-6 and BTV-8 by real-time and conventional RT-PCR,

 but only BTV-6 was isolated : see  GER2008/01

Germany

Received at IAH  approximately  12th Dec 2008

Positive field cattle blood

  Animal No. BH266/1 original blood sample

Bernd Hoffmann

 

A176/08 1
Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman

Used to generate
 
GER2008/01

GER2008/05

BTV-6

 

Identified by BTV-6 specific real time PCR LSI assay

Germany

 

(sample taken

2008)


Received at IAH  approximately  21st Jab 2009

blood from experimentally infected cattle

 

Animal No. 15

(12 dpi)

Blood sample from experimentally infected cattle

 

 used to generate isolate  GER2008/04,

GER2008/12

&

GER2008/14

Bernd Hoffmann

 

Note : only 9 vials (P)


[Stars no. A176/08 3]
 

[ S2:  GQ506461,  S4: GQ506462,
 S5: GQ506463,   S6: GQ506464,
S7: GQ506465,   S8: GQ506466,
S9: GQ506467 ]


Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman
GER2008/04

BTV-6

 

Identified by BTV-6 specific real time PCR LSI assay

Germany

Received at IAH  approximately  12th Dec 2008

blood from experimentally infected cattle

Shelf B8,

 box B8 β,

 rows I1-J10

 

(Added to collection

09th Feb 2009)

Animal

No. 15

 

(12 days post infection)
[IAH/KC2])

Derived from GER2008/05

Bernd Hoffmann


[Stars no. A176/08 3]
Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman
GER2008/03

BTV-6

 

Identified by BTV-6 specific real time PCR LSI assay

Germany

Received at IAH  approximately  12th Dec 2008
Vero cell culture isolated at FLI Riems

Shelf B7,

 box B7 β,

 rows A1-B10

 

(Added to collection
 16th Jan 2009)

Animal No. 15

7 dpi

V2 (IAH V1 )

Bernd Hoffmann

A176/08 4
Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman
GER2008/02

BTV-6

 

Identified by BTV-6 specific real time PCR LSI assay

Germany

Received at IAH  approximately  12th Dec 2008

Positive field cattle blood

Shelf B7,

 box B7 α.

 rows I1-J10

 

(Added to collection
 16th Jan 2009)
Animal No. BH266/2 [IAH/KC1]

Bernd Hoffmann

A176/08 2
Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman

Derived from blood sample
GER2008/07
GER2008/01

BTV-6

 

Identified by BTV-6 specific real time PCR LSI assay

Germany

Received at IAH  approximately  12th Dec 2008

Positive field cattle blood

Shelf B,

 box B7 α,

 rows G1-H10

 

(Added to collection
 16th Jan 2009)
Animal No. BH266/1 [IAH/KC1]

Bernd Hoffmann

A176/08 1
Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman

Derived from blood sample
GER2008/06
NET2008/17

BTV-6 and

BTV-8

 

Identified by seg-2 specific RT-PCR

Netherlands:

Luttenburg

Cattle blood

 

Shelf B17,

box B17 γ ,

rows H1-H2

 

(Added to collection

29th  July 2010)

 

Blood sample

 from cattle

 (no passage

 history)

 

Used to generate

 isolate 

NET2008/15

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 1]

ProMed Report

related to BTV-6 vaccine strain

  

Maan et al (2010)

NET2008/16

BTV-6

 

Identified by seg-2 specific RT-PCR

BTV-6

 

Identified by seg-2 specific RT-PCR

from cattle blood

 

Isolated at

IAH-P

7th Nov 2008

    from cattle
blood sample

[IAH/KC2]

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 4]

ProMed Report

related to BTV-6 vaccine strain

 

Maan et al (2010)

NET2008/15

BTV-6 and

BTV-8

 

Identified by seg-2 specific RT-PCR

Netherlands:

Luttenburg

from cattle blood

 

Isolated at

IAH-P

7th Nov 2008

    from cattle
blood sample

[IAH/KC2]
Derived from
blood sample
NET2007/17

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 1]

ProMed Report

related to BTV-6 vaccine strain

 

Maan et al (2010)

NET2008/14

BTV-6

 

Identified by seg-2 specific RT-PCR

Netherlands:

Heeton

from cattle blood

 

Isolated at

IAH-P

7th Nov 2008

    from cattle
blood sample

[IAH/E1/BHK2]

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 3]

ProMed Report

related to BTV-6 vaccine strain  

 

Maan et al (2010)

NET2008/13

BTV-6

 

Identified by seg-2 specific RT-PCR

Netherlands:

Barchem

from cattle blood

 

Isolated at

IAH-P

7th Nov 2008

    from cattle
blood sample

[IAH/E1/BHK2]

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 4]

ProMed Report

related to BTV-6 vaccine strain

 

Maan et al (2010)

NET2008/12

BTV-6

 

Identified by seg-2 specific RT-PCR

Netherlands:

Barchem

from cattle blood

 

Isolated at

IAH-P

18th Nov 2008

Shelf A17,

box A17 γ,

rows A1 B10

added to collection

20th Nov 2008

  from cattle
blood sample

[IAH KC2/BHK1]

NET2008/08)

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 4]

ProMed Report

related to BTV-6 vaccine strain

 

 

Maan et al (2010)

NET2008/10

BTV-6

 

Identified by seg-2 specific RT-PCR

Netherlands:

Barchem

blood

from cattle

 

(isolated at
IAH P
7th Nov 2008)

Shelf A17,

box A17 beta,

rows C1 D10

 

 added to collection

 7th Nov 2008 

  from cattle
blood sample

[IAH/E1/BHK2]

(Derived from
NET2008/08)

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 4]

ProMed Report

related to BTV-6 vaccine strain  

 

 

Maan et al (2010)

NET2008/08
(virus isolate)
BTV-6 Netherlands 2008   Shelf A17,
Box A17 alpha,
Rows I 1 to J10
     
NET2008/08
(blood sample)

BTV-6

 

Identified by seg-2 specific RT-PCR and sequence analyses

Netherlands:

Barchem

 

Shelf B17,

box B17 γ ,

rows F9

 

(Added to collection

28th  July 2010)

 

Blood sample from cattle

 

(no passage history)

 

Used to generate isolate  NET2008/10

&

 NET2008/12

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 4]

ProMed Report

related to BTV-6 vaccine strain

 

Maan et al (2010)

 

See also blood sample NET2008/09

(Mixed BTV-6 and 8)

and

NET2008/06

(BTV-6 blood sample)

NET2008/07

BTV-6

 

Identified as BTV-6 by conventional RT-PCR (three primer sets) and sequence analysis. 

Netherlands:

Heeton

 

September 2008

 

from cattle blood

 

Isolated at

IAH-P

27th Oct 2008

Shelf A17,

Box A17 alpha,

rows G12 to H10

&

Shelf B10,

box B10 α,

rows I1 J10 

 

Added to collection on

18th Sept 2009 

 

[IAH/KC2]

 

(Derived from

NET2008/04)

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 3]

 

 

 

Seg-2 of this isolate is closely  related to that of the South African reference strain of BTV-6

 ( >99% identity)

 

ProMed Report

BTV-6 (vaccine strain)

 

Maan et al (2010)

NET2008/06

BTV-6

 

Identified by seg-2 specific RT-PCR and sequence analyses

Netherlands:

Heeton

 

Shelf B17,

box B17 γ ,

rows G9-10

 

(Added to collection

28th  July 2010)

 

Blood sample from cattle

 

(no passage history)

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 3]

ProMed Report

BTV-6 (vaccine strain)

 

[ S2:GU550957, 

S5: GQ506482, 

S6: GQ506483, 

S7: GQ506484,

 S8: GQ506485,

S9: GQ506486,

 S10: GQ506487 ]

Maan et al (2010)

NET2008/05

BTV-6

(to be confirmed)

Netherlands:

Heeton

 

September 2008

from cattle blood

 

Isolated at

IAH-P

27th Oct 2008

Shelf A17,

box A17 α,

rows C1 F10 

 

Added to collection on

(5th Nov 2008)

 

[IAH/KC1/BHK1]

Supplied by Piet Van Rijn

CIDC Lelystad

 

[Stars No.  A163/08 no 3]

ProMed Report

BTV-6 (vaccine strain)  

*

Duplicate vials  were removed by Narender Maan to be sent  to Fort Dodge 31st March 2009.

*

Vial A17C2(g) A&B sent to Evert Jor,

National Veterinary Institute Norway,

 on 31st March 2009

*

Vial position D2 was removed and given to C. Batten IAH-P, on 25th Aug 2009 to grow material for VLA for PCR validation, Duplicate vial left in Misc box position H8

*

 

 [Seg-1: GQ506472

Seg-2: GQ506473,

 Seg-3:  GQ506474

Seg-4:  GQ506475,

 Seg-5:  GQ506476,  

Seg-6:  GQ506477,

 Seg-7:  GQ506478

Seg-8:  GQ506479,

Seg-9:  GQ506480

Seg-10:  GQ506481

Maan et al (2010) (4)]

NET2008/04

BTV-6

 

Identified as BTV-6 by conventional RT-PCR (three primer sets) and sequence analysis. 

Netherlands:

Heeton

 

September 2008

 

from cattle blood

 

Isolated at

IAH-P

27th Oct 2008

Shelf A17,

box A17 α,

rows A1 B9

 

 

 

[IAH/KC1]

 

Used to generate 25th Jan 2010  (NET2008/07)

 

 Seg-2 of this isolate is very closely  related to that of the South African reference strain of BTV-6
( >99% identity)

Supplied by Piet Van Rijn

 CIDC Lelystad

 

[Stars No.  A163/08 no 3]

  

Vials A8 were sent to Juan de Plana Fort Dodge, in Spain, 21st Jan 2009.

*

Vial A9 (duplicate) sent Merial Lyon France (Pascal Hudelet) 3rdSept 2009

* 

Additional vials , were added to restock positions rows A1 B8 and B10 25th Jan 2010 .

 

*

Vial, A1 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th April 2013 for sequencing. Corresponding vial in freezer B  was  not removed
*
 

ProMed Report

BTV-6 (vaccine strain) ;

 

Maan et al (2010)

 

[ S1: GQ506468,   S2:GU550956,
 S3: GQ506469,   S4: GQ506470,
S7: GQ506471 ]

USA2006/02

BTV-6

 

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-6 antisera)

Okeechobee  County, FL,

USA

 

(sample taken

11th Oct 2006)

From cattle

 

(no clinical history)

 

Date of virus

 preparation in USA

not given

Shelf E3,

box E3β,

rows I1 J10 

 

Added to collection

17th Sept 2007 

06-461278-5

 

(tube labelled: 461278-5;

 IVD BHK/1;

10-30-06)

From blood

 

E1/BHK1

[IAH/ BHK3]

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010 USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

 

Blood originally  taken for testing to clear animals for export to Brazil

USA2006/01

BTV-6

 

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-6 antisera)

Okeechobee  County, FL,

USA

 

(sample taken

11th Oct 2006)

From cattle

 

(no clinical history)

 

Date of virus

 preparation in USA

not given

Shelf E3,

box E3β,

rows A1 B10 

 

Added to collection

17th Sept 2007 

06-461278-3

 

(tube labelled: 461278-3;

 IVD BHK/1;

10-30-06)

From blood

 

E1/BHK1

[IAH/ BHK3]

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office. PO box 844

Ames .IA 50010  USA

* 

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

*

Vial, A1 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th April 2013 for sequencing. Corresponding vial in freezer B  was  not removed
*

[S1: GQ506536, 

S2: GQ506537,

S3: GQ506538, 

S4: GQ506539,

S5: GQ506540, 

S6: GQ506541, 

S7: GQ506542,

S8: GQ506543,

S9: GQ506544,

S10:  GQ506545]

 

Blood originally  taken for testing to clear animals for export to Brazil

 

References
1.

 Mertens PP, Maan NS, Prasad G, Samuel AR, Shaw AE, Potgieter AC, Anthony SJ, Maan S. (2007 ) Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains. J Gen Virol. 88, 2811-2823.
2  
 
Shaw AE, Monaghan P, Alpar HO, Anthony S, Darpel KE, Batten CA, Guercio A, Alimena G, Vitale M, Bankowska K, Carpenter  S, Jones H, Oura CA, King DP, Elliott H, Mellor PS, Mertens PP. (2007 Development and initial evaluation of a real-time RT-PCR assay  to detect bluetongue virus genome segment 1.) J Virol Methods.145, 115-126.
 
3.   


 Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC (2007) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes. J Gen Virol. 88, 621-630.

4.

Sushila Maan, Narender S. Maan, Piet A.van Rijn, Ren G.P. van Gennip, Anna Sanders, Isabel M. Wright, Carrie Batten, Bernd Hoffmann, Michael Eschbaumer, Chris A.L. Oura, Abraham. C. Potgieter, Kyriaki Nomikou, and Peter P.C. Mertens (2010) Full genome characterisation of bluetongue virus serotype 6 from the Netherlands 2008 and comparison to other field and vaccine strains PLoS One. 2010 Apr 23;5(4):e10323. doi: 10.1371/journal.pone.0010323.

   

Hit Counter