ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 8 (BTV-8)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection » Contents

 

 

 

 

Family: Reoviridae,  Genus: Orbivirus,  Species:   Bluetongue virus type 8 (BTV)
Virus Species

 * IAH dsRNA virus collection number

Serotype

 

 

(identification method)

Place of original sample

e.g. country town

[grid reference]

 

(date original sample was taken)

Isolated from which host species

e.g cow,  sheep, etc

 

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

 

(Date added to collection)

(Suppliers Isolate Designation)

Adapted to which cell types

 

 

Original sample material

 

 

(Passage history of sample)

* Original sample supplied to the collection by

person / institution

 

[IAH virus diagnosis, ISIS sample number]

Comments

References

&

 accession numbers

 

[genome segments that

have been sequenced]

RSArrrr/08


















BTV-8

 

(reference strain- 'western' topotype)

Supplied by OVI

S. Africa*

(17/12/82)

NK

Shelf B3,

Box B3γ,

Rows G1-H10

 

 (Added to collection on 20th May 2005)

(Ref. Strain)

E2/BHK6

 

[IAH/BHK1]

*

Duplicate samples G1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

Duplicate samples,  box B3γ- position H1(p) supplied to Oscar R. González Llamazares (Avenida Párroco Pablo Díez 49-57, 24010 - Leon - Spain,  22nd Nov 2007)

* 

Vial H2 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial H3 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

* 

Vial G sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

*

[Seg-1: KP820956,

Seg-2: KP821076,

Seg-3: KP821198,

Seg-4: KP821318,

Seg-5: KP821438,

Seg-6:  KP821558,

Seg-7: KP821680,

Seg-8: KP821800,

Seg-9: KP821920,

Seg-10: KP822041,

Nomikou et al 2015]

 

 

[S2:AJ585129,

S6:AJ586705]

Maan et al 2007

 

 

» contents

2010
ISR2010/20

BTV-8 Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR

Israel

Meishar

September 2010

Isolated from sheep blood at IAH-P

Shelf C14,

box  C14α,

rows E1-F10

 

Added to collection  04/07/11

2646/10/3

KVI Id 84994

 [IAH/KC1]

Jacob Brenner

Kimron Institute Israel

 

 Samples arrived 6TH April 2011

 

IAH Stars No.

A32/11 12

Note original blood positive

 for BTV-4 and 8

ISR2010/18

BTV-8 Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR 

20/05/10 Bet Chilkia, Israel

Isolated from cattle blood at Kimron through eggs and vero

Shelf C12

box  C12 α,

 rows G and H

 

Added  17/1/11

1610

E1/V1 [V1 at IAH-P]

Jacob Brenner

Kimron Institute Israel

 

 Samples arrived

 24th Nov 2010

 

IAH Stars No. 

A114/10 17

ISR2010/17

BTV-8 Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR 

07/11/10 Tzipori, Israel Sheep blood

Shelf C12,

box  C12 α,

 rows A and B

 

Added  17th Jan 2011

2944-3 KC1

Jacob Brenner, Kimron Institute Israel

  

Samples arrived 24th November 2010

 

IAH Stars
number
A114/10 16
Vial J10 was not added to
 freezer B
ISR2010/13

BTV-8 Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR 

10/10/10

Ein Kamonin, Israel
Caprine blood

Shelf C12

box  C12 alpha rows C and D

 

Added  17/1/11

2717 KC1

Jacob Brenner, Kimron Institute Israel

Samples arrived 24th November 2010

 

IAH Stars
number
A114/10 10
 
ISR2010/12

BTV-8 Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR 

13/10/10

Bet Kama, Israel
Cattle blood

Shelf C12

Box  C12 alpha Rows E and F

 

Added  17/1/11  

2760 KC1

Jacob Brenner, Kimron Institute Israel

Samples arrived 24th November 2010

 

IAH Stars
number
A114/10 09
Vial F10 was not added to
 freezer A & B
ISR2010/10

BTV-8 Identified as BTV-8 by Seg-2 specific

 real time RT-PCR 

Dvir, Israel Cattle blood

Shelf C11

box  C11gamma, rows  G and H

 

 

Added  14/1/11
2800 KC1

Jacob Brenner, Kimron Institute Israel

 

 Samples arrived 24th November 2010

 

IAH Stars
number
A114/10 07
 
ISR2010/08

BTV-8 Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR 

30/8/10

Yoqneam, Israel
Sheep blood (Sheep had classical signs of BTV

Shelf C11

Box  C11 gamma,

 Rows  C and D (note only 9 pdam in B)

 

Added  14/1/11
2089/1 KC1

Jacob Brenner, Kimron Institute Israel

Samples arrived 24th November 2010

 

IAH Stars
number
A114/10 01
Vial D10 was not added to
 freezer B
ISR2010/07

BTV-8

 

 

Identified as BTV 8 by Seg-2 specific

 Real time RT-PCR 

Israel,

 From Ashdot

 

Sample taken on

27th June 2010
Isolated in KC cells at IAH

Shelf C9,

box α,

rows E1-F10

 

Added to collection

31st Aug 2010

1788 27.06.10 from bovine
blood

 
[IAH/KC1]

Supplied by Jacob Brenner , Kimron Institute Israel

 

Samples arrived 4th August 2010

IAH Stars No.
A73/10 09
ISR2010/06

BTV-8

 

 

Identified as BTV 8 by Seg-2 specific

 Real time RT-PCR 

Israel,

 From Hadera,

From sheep blood

 

 sampled

21st June 2010
Isolated in embyonated chicken eggs at Kimron and
grown in BHK cells at IAH-P

Shelf C7

box α,

rows E1-F10

 

added 20th Aug 2010
1750 20.0610

E1

[IAH/BHK1]

Jacob Brenner , Kimron Institute Israel

 

Samples arrived

4th Aug 2010

IAH Stars
number
A73/10 06
Infected lamb from
a nomadic flock
2009
FRA2009/03 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2009

Shelf C10,

box C10 γ ,

rows C1-D10

 

Added to collection

8th Oct 2010

Original tube marked as
BTV8.09.03
BHK

[IAH/BHK1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
vaccination status of animal unknown

Note: Only three isolates of BTV-8 were made in France during 2009

Original sample stored at in misc box bottom freezer A
FRA2009/02 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2009

Shelf C10,

box C10 γ ,

rows A1-B10

 

Added to collection

8th Oct 2010

Original tube marked as
BTV8.09.02
BHK

[IAH/BHK1 ]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
vaccination status of animal unknown

Note: Only three isolates of BTV-8 were made in France during 2009

Original sample stored in Misc box bottom of freezer A
FRA2009/01 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2009

Shelf C10,

box C10 β ,

rows I1-J10

 

Added to collection

8th Oct 2010

Original tube marked as
BTV8.09.01
BHK

[IAH/BHK1 ]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Vial I1 removed from freezer B

vaccination status of animal unknown

Note: Only three isolates of BTV-8 were made in France during 2009

Original sample stored in Misc box bottom of freezer A
ISR2009/14

BTV-8

 

Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR 

Israel,

 

 from Ayelet Ha Shahar

 

sampled

22nd July  2009
From Cattle 
blood

Blood put direct onto KC cells at IAH-P

Shelf A17

Box  A17 γ

Rows E1-F10

 

 Added to
collection on
1st  Sept 2009

Cow number 582 [IAH/KC1]

From: Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

16th August  2009

IAH Stars number

A71/09 3
 
ISR2009/13

BTV-8

 

Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR 

 

Israel,

 

 from Ayelet Ha Shahar

 

sampled

22nd July  2009
From Cattle

Isolated through eggs in Israel,
 passed to KC cells at IAH-

Shelf A17

Box  A17 γ

Rows C1-D10

 Added to
collection on
1st  Sept 2009
No Cow ID

E1

[IAH/BHK2]

From: Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived 16th August 2009

 

IAH Stars number
A71/09 10
 

OMN2009/04

BTV-8

 

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

Oman from Sohar, Batnah coast in the Arabian Gulf

Sample collected 13th April 2009

ovine blood

Isolated in KC cells at IAH-P

Shelf B9

Box B9 alpha

Rows Added  G1- H10

Oman designation 16441

[IAH/KC1]

Masha Japhet,

Oman

 

Samples arrived

15TH May 2009

 

IAH Stars No. 
A42/09 37
Not all +ve animals showed clinical signs of disease. Only Wiltshire sheep, Arabian gazelles, fallow deer and blackbucks were affected . Only one multi-horn sheep died, showing similar signs and post mortem changes, but all of the other live animals sampled were -ve. The +ve Cattle, buffalo, goats and reem gazelles showed no signs of disease .
Strains of  BTV-1, BTV-4 and BTV-16 were also isolated in early 2009
(see: 
OMN Isolates)
OMN2009/03

BTV-8

Identified as BTV by Seg-7 specific

 Real time RT-PCR

 

Identified as BTV-8  by Seg-2 specific

 Real time and conventional RT-PCR, and sequencing

Oman from Sohar, Batnah coast in the Arabian Gulf

Sample collected
28th Apr 2009

Sheep blood

 

Isolated in

KC cells

at IAH-P

Shelf B9,

Box B9 α,

Rows  E1- F10
Oman designation F1644 [IAH/KC1]

Masha Japhet,

Oman

 

Samples arrived

Approx 15TH May 2009

 

IAH Stars No.
A42/09 21
Not all +ve animals showed clinical signs of disease. Only Wiltshire sheep, Arabian gazelles, fallow deer and blackbucks were affected . Only one multi-horn sheep died, showing similar signs and post mortem changes, but all of the other live animals sampled were -ve. The +ve Cattle, buffalo, goats and reem gazelles showed no signs of disease .
Strains of  BTV-1, BTV-4 and BTV-16 were also isolated in early 2009
(see: 
OMN Isolates)

*
Sample E2 given to Kyriaki Nomikou for sequencing (IAH-P) 17th Aug 2009
*

Vial position F1(g) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013.  Corresponding vial in Freezer B has not been removed.

*

2008

DEN2008/02

BTV-8

 

Identified by LSI real time BTV 8 assay

From cattle

blood

 

Denmark  

Sample taken  from a calf

Originally isolated at IAH-Pirbright, from cattle blood

 

(sampling date 29th Aug 2008)

Shelf A12

Box A12 β,

rows  I1-J10

 

(Added to collection  26th Sept 2008)

 

[IAH/KC1]

[Stars No.

A150/08 2]

 

DEN2008/01

BTV-8

 

Identified by LSI real time BTV 8 assay

From cattle

blood

 

Denmark  

Sample taken  from a cow

Originally isolated at IAH-Pirbright, from cattle blood

 

(sampling date 29th Aug 2008)

Shelf A12,

Box A12 β,

rows G1-H10

 

(Added to collection on  26th Sept 2008)

cow (009)

[IAH/KC1]

[Stars No.

A150/08 1]

*
Vial position G1 (g) taken out of freezer A and given to Eva Veronesi  (IAH) on 27th Sept 2010. The corresponding (spare) vial in freezer B was not removed
*
FRA2008/35

BTV-8

Identified by  real time PCR
France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf D18    

Box  D18 β

Rows A1-10

 

(Added to

collection on 10/06/2015)
Original tube marked as BTV8.08.13  

BHK?

[TPI/BHK1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France

23rd July 2010

as BHK cell culture material

from a non-vaccinated

animal
FRA2008/20 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Original tube marked as
BTV8.08.35
BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/19 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008


Original tube marked as BTV8.08.34
BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/18 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008


Original tube marked as BTV8.08.33
VERO Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as VERO cell culture material
from a non-vaccinated
animal
FRA2008/17 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf D18   

Box  D18 α

Rows I1-10

 

(Added to

collection on 10/06/2015)

Original tube marked as BTV8.08.32
BHK?
[TPI/BHK1]
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/16 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008


Original tube marked as BTV8.08.29
BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/15 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf D18   

Box  D18 α

Rows J1-10

 

(Added to

collection on 10/06/2015)

Original tube marked as BTV8.08.26
BHK?
[TPI/BHK1]
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
 23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/14 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Original tube marked as BTV8.08.22 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/13 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Original tube marked as BTV8.08.19 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/12 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Original tube marked as BTV8.08.18 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/11 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Original tube marked as BTV8.08.17 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
 23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/10 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf D17,

Box D17 γ,

rows B1 to B10

 

(Added to

collection on

5th Dec 2014, by E.M.)

Original tube marked as BTV8.08.16 CPAE1 Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/09 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf D17,

Box D17 γ,

rows A1 to A10

 

(Added to

collection on

5th Dec 2014, by E.M.)

Original tube marked as BTV8.08.15
BHK1
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/08 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf    D17

Box   D17ɑ

Rows J1-J10

 

(Added to the

collection on

25st Nov 2014)

Original tube marked as BTV8.08.12 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/07 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf    D17

Box   D17ɑ

Rows I1-I10

 

(Added to the

collection on

25st Nov 2014)

Original tube marked as BTV8.08.11 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
 23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/06 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Original tube marked as BTV8.08.10 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/05 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf    D17

Box   D17ɑ

Rows H1-H10

 

(Added to the

collection on

 24st Nov 2014)

Original tube marked as BTV8.08.09 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/04 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf    D17

Box   D17ɑ

Rows G1-G10

 

(Added to the

collection on

24st Nov 2014)

Original tube marked as BTV8.08.08 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/03 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf   D17

Box   D17ɑ

Rows F1-F10

 

(Added to the

collection on

24st Nov 2014)

Original tube marked as BTV8.08.07 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
 23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/02 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf    D17

Box   D17ɑ

Rows E1-E10

 

(Added to the

collection on

24st Nov 2014)

Original tube marked as BTV8.08.06

BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal
FRA2008/01 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2008

Shelf D17,

Box D17 β,

row J1 to J10

 

(Added to

collection on

5th Dec 2014, by E.M.)

Original tube marked as BTV8.08.01
CPAE1
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010
as BHK cell culture material
from a non-vaccinated
animal

GER2008/13

BTV-8

 

Identified by seg-2 specific real-time RT-PCR

From Germany

 

(sample taken

2008)

Positive blood from

field cattle

Shelf B18,

box B18 β ,

rows C1-D10

 

(Added to collection

29th  July 2010)

from
Animal No.
BH287/08_25

[IAH/KC1]

Derived from

GER2008/11

From Dr. Bernd Hoffman

Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman

GRE2008/12 BTV-8 collected in 2008  

Shelf C17,

box C17 Beta ,

rows C1-D10 

       
GER2008/11

 BTV-8

 

Identified by seg-2 specific real-time RT-PCR

From Germany

 

(sample taken

2008)

 

Received at IAH 21st Jan 2009 
Positive blood from field cattle

Shelf B17,

box B17 γ ,

rows G1-G3

 

Added to collection

9th  April 2010

BH287/08_25 Blood sample

Used to generate
GER2008/13

From Dr. Bernd Hoffman

 

Received at IAH 

21st Jan 2009 

Note: Only 3 vials (P)

Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman
GER2008/10

BTV-8

 

Identified by seg-2 specific real-time RT-PCR

From Germany

 

(sample taken

2008)

 

Positive blood from field cattle

Shelf B17,

box B17 γ ,

rows G5-G7

 

Added to collection

9th  April 2010

BH287/08_33 Blood sample From Dr. Bernd Hoffman


Received at IAH 21st Jan 2009 
Note: Only 3 vials (P)

Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman

GRE2008/01

BTV-8

 

Identified by seg-2 specific RT-PCR

Greece:

Agia Paraskevi

 

Herd No:

EL83306344

from sheep

blood

 

Isolated at

IAH-Pirbright

1st Dec 2008

Shelf A18,

box A18 β,

rows  I1 – J10

 

(added to collection

 10th Dec 2008)

Animal ID:

EL833063440340

[IAH/KC1]

Supplied by Olga Mangana

 

[Stars No. 

 A169/08 no 1]

*

Vial position J1(p) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013.  Corresponding vial in Freezer B has not been removed.

*

ISR2008/13

BTV-8

 

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

Israel,

 from a village called: Rosh ha-nikra

 

samples

27th Nov 2008

From bovine spleen,

 

Isolated in KC cells at IAH-P

Shelf B8, 

box B8γ,

Rows  C1-D10

 Added to collection on 
8th June 2009

Kimron institute

number

593223
[IAH/KC2]

Dr. Hagai Yadin, Kimron Institute Israel.

 

Samples arrived

Approx

14th  April 2009

IAH Stars No.
A25/09 9
 

NET2008/03

BTV-8

From cattle blood

Netherlands:  Staphorst  region

 

7th Aug 2008

 ~1yr old, unvaccinated animal

 

clear clinical signs of BT, including 

fever and conjunctivitis

 Shelf A14

Box A14 β,

Rows A1-B10

 

(Added to collection 

16th Oct 2008)

(Animal ID 08016384)

[IAH/KC1/BHK1]

 

Derived from NET2008/01

Dr. Piet A. van Rijn, PhD

 

CVI Wageningen UR, Department of Virology P.O. Box 65, 8200 AB Lelystad, The Netherlands

First case detected in the Netherlands 2008

 

Was seropositive on first sampling 1st Aug 2008

 

Resampled for virus isolation on  7th Aug 2008

 

*

Vial position A1 (P)  from freezer A and B given to K. Nomikou (IAH-VBDP) 16th Dec 2009

*

Vials A2, A3, A4 (A+B) given to K. Nomikou (IAH-VBDP) 23rd Feb 2010

*

NET2008/02

BTV-8

From Sheep blood (unvaccinated)

 

Netherlands: Bierum region

sample taken

 

15th Aug 2008.

clear clinical signs of BT

Shelf A14

Box A14 β,

Rows C1-D10

 

(Added to collection  16th October 2008

 

(Animal ID 08017118-001)

[IAH/KC1]

Dr. Piet A. van Rijn, PhD

 

CVI Wageningen UR, Department of Virology P.O. Box 65, 8200 AB Lelystad, The Netherlands

Was seropositive

on first sampling

on 14th Aug 2008. 

 

Resampled

15th Aug 2008.

 

This was the 2nd  case of BTV-8 in NET 2008.

NET2008/01

BTV-8

From cattle blood

Netherlands:  Staphorst  region

 

7th Aug 2008

 ~1yr old, unvaccinated animal

 

clear clinical signs of BT, including 

fever and conjunctivitis

 Shelf A14

Box A14 β,

Rows E1-F10 

 

(Added to collection  16th October 2008)

(Animal ID 08016384)

[IAH/KC1]

 

Used to generate NET2008/03

Dr. Piet A. van Rijn, PhD

 

CVI Wageningen UR, Department of Virology P.O. Box 65, 8200 AB Lelystad, The Netherlands

First case detected in the Netherlands 2008

 

Was seropositive on first sampling 1st Aug 2008

 

Resampled on

7th Aug 2008

SWE2008/02

BTV-8

 

Identified by LSI real time BTV 8 assay

Sweden 

 

From cattle

blood

Originally isolated at

IAH-Pirbright

7th Oct 2008

Shelf A14,

box A14 α ,

rows G1-H10

 

(added to collection

 9th Oct 2008)

Sample ID: 155300

[IAH/KC1]

samples supplied  by  Peter Thorén

Department of Virology, Immunobiology and Parasitology,

 Diagnostic Section

National  Veterinary Institute, SVA

 

[Stars No.

A157/08 1]

 

SWE2008/01

BTV-8

 

Identified by LSI real time BTV 8 assay

 

Sweden

 

From cattle

blood

Originally isolated at

IAH-Pirbright

7th Oct 2008

Shelf A14,

box A14 α ,

rows E1-F10

 

(added to collection

 9th Oct 2008)

Sample ID: 152951

[IAH/KC1]

samples supplied  by  Peter Thorén

Department of Virology, Immunobiology and Parasitology,

 Diagnostic Section

National  Veterinary Institute, SVA

 

[Stars No.

A157/08 1]

 
UKG2008/52

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

 Sample taken  from a cow that was imported 4th Sept 2008 into Rushford (Newcastle) England, England from central Germany

originally isolated at IAH-Pirbright from cattle blood

 

(sampling date 6th Sept 2008)

Shelf B16

box B16β

rows C

tube C4 & C5

 

Added to collection

23rd Oct 2008

Animal id DE0348663886

blood sample

[Stars No.

B13469/08 12]

Used to generate isolate UKG2008/17

 

Two tubes in freezer A and one  in freezer B

UKG2008/51

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

 Sample taken  from a cow that was imported 4th Sept 2008 into Rushford (Newcastle) England, England from central Germany

originally isolated at IAH-Pirbright from cattle blood

 

(sampling date 6th Sept 2008)

Shelf B16

box B16β

rows C

tube C1 & C2

 

Added to collection

23rd Oct 2008

Animal id DE0350330361

blood sample

[Stars No.B1369/08 13]

Used to generate isolate UKG2008/16

 

Two tubes in freezer A and one  in freezer B

UKG2008/50

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken  from a cow imported on

19th Aug 2008

 into Tiverton England

from central Germany

Originally isolated at IAH-Pirbright from cattle blood

 

(sampling date 29th Aug 2008)

Shelf B16

box B16β

rows B

tube B9

Added to collection

23rd Oct 2008

Animal Id DE0348929473

blood sample

[Stars No. B1346/08 33] 

Used to generate isolate UKG2008/15

 

single  tube in freezer A & B

UKG2008/49

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

 From sheep

blood

 

Sample taken  from a sheep that was imported into Hertfordshire

England on

19th Aug 2008,

from central France

Originally isolated at 1AH-Pirbright

 from sheep blood

 

(sampling date 29th Aug 2008)

Shelf B16

box B16β

rows B

tube B7

 

Added to collection on 13th Oct 2008

No animal id,

south down sheep

blood sample

[Stars No.  B1340/08 1]

Used to generate isolate UKG2008/14

 

single  tube in freezer A & B

UKG2008/48

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

 

(sampling date 29th Aug 2008)

 

Shelf B16

box B16β

rows B

Tubes B4 to B5

 

Added to collection on 13th Oct 2008

Animal id DE0348929478

Blood sample

[Stars No.  B1346/08 35]

Used to generate isolate UKG2008/13

 

Two tubes in freezer A & B

UKG2008/47

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

 

 

(sampling date 29th Aug 2008)

Shelf B16

box B16β

rows B

Tubes B1 to B2

 

Added to collection on 13th Oct 2008

Animal id DE0348682705

Blood sample

[Stars No.  B1346/08 30]

Used to generate isolate UKG2008/12

 

Two tubes in freezer A & one in freezer B

UKG2008/46

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbrightf rom cattle blood

 

(sampling date 29th Aug 2008)

 

Shelf B16

box B16β

rows A

Tubes A7 to A8

 

Added to collection on 13th Oct 2008

Animal id DE0350610251

Blood sample

[Stars No.  B1346/08 21]

Used to generate isolate UKG2008/11

 

Two tubes in freezer A & B

UKG2008/45

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

 

(sampling date 29th Aug 2008)

Shelf B16

box B16β

rows A

Tubes A4 to A5

 

  

Added to collection on 13th Oct 2008

Animal id DE0348684975

Blood sample

[Stars No. B1346/08 20]

Used to generate isolate UKG2008/10

 

Two tubes in freezer A & B

UKG2008/44

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

 

(sampling date 29th Aug 2008)

Shelf B16

box B16β

rows A

Tubes A1 to A2

 

Added to collection on 13th Oct 2008

Animal id DE0348986505

Blood sample

[Stars No.  B1346/08 17]

Used to generate isolate UKG2008/09

 

Two tubes in freezer A & B

UKG2008/43

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

 cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

(sampling date 29th Aug 2008)

Shelf B16

box B16α

rows J

Tubes J1 to J2

 

Added to collection on 13th Oct 2008

 

Animal Id DE0348986502

Blood sample

[Stars No. B1346/08 16]

 

Used to generate isolate UKG2008/08

 

Two tubes in freezer A & B

UKG2008/42
(virus isolate)
BTV-8 UK
2008
virus isolate Shelf D8
Box D8 Beta, Rows I1 to J10
       

UKG2008/42

(blood sample)

BTV-8

 

Identified by serotype specific real time RT-PCR assay (LSI)

 sheep

blood

 

Sample taken  from a ram that was imported on 19th Aug 2008 to Lewes in Sussex,  England,  from central France

Originally isolated at 1AH-Pirbright from sheep blood

 

(sampling date 20th Aug 2008)

 Shelf B16

box B16α

rows I

Tubes I1 to I3

 

Added to collection on  5th Sept 2008

South Down Sheep

 

(no animal Id number)

blood sample

[B1336/08 1 ]

Used to generate isolate UKG2008/07

 

Three tubes in freezer A & B

UKG2008/41

BTV-8

 

Identified by group specific RT-PCR  (1)

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

 and sequence analyses (3)

Sawston hall hotel

from calf blood

Calf born 19/02/08

 

Animal ID.

UK226770100010

[IAH/

KC1/BHK1]

[Stars No.

B687/08 2]

 

UKG2008/40

BTV-8

 

Identified by LSI real time BTV-8 assay

From cattle

blood

England

 

Sample taken  from a cow that was imported on 04/9/08 into Bromyard, England, from central Germany

Originally isolated at IAH-Pirbright

 

 

(sampling date 8th Sept 2008)

Shelf A14

box A14 α,

rows C1-D10

 

added to collection on

9th Oct 2008

 

From cattle blood

 

 [IAH/KC2]

B1380/08 21 

Animal Id 62578

 
UKG2008/39

BTV-8 (blood)

   

Shelf B16,

box B16 b,

rows H2

 

Added to collection on

(25th March 2010)   

only 1 vials (G) for freezer A

UKG2008/38

BTV-8 (blood)

Shelf B16,

box B16 b,

rows D1-D5

5 in freezer A (D1-D5),

4 in freezer B (D1-D4)

UKG2008/37

BTV-8 (blood)

Shelf B16,

box B16 b,

rows D8-D10

3 in freezer A (D8-D10),

4 in freezer B (D7-D10)

UKG2008/36

BTV-8 (blood)

Shelf B16,

box B16 b,

rows E2-E3

2 in freezer A (E2-E3),

3 in freezer B (E1-E3)

UKG2008/35

BTV-8 (blood)

Shelf B16,

box B16 b,

rows H1

 

Added to collection on

(25th March 2010)  

only 1 vials (G) for freezer A

UKG2008/34

BTV-8

 

Identified by group specific RT-PCR  (1)

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

 and sequence analyses (3)

From cattle

blood

 

Armoy, Ballymena,

Northern Ireland

 

Sample taken

13th Feb 2008

 

from calf blood

Calf born 19/02/08

Originally isolated at 1AH-Pirbright

 

From cattle blood

(26th Feb 2008)

Shelf A16

Box A16 gamma,

Rows F1-G10

 

[IAH/

KC1/BHK1]

 

 

This calf was born to animal number B492/08 1, represented by virus isolate number

UKG2008/06

and

UKG2008/32

[Stars No.

B492/08 2]

 

UKG2008/33

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal

UK582544501188

 

Armoy, Ballymena,

Northern Ireland

 

(Sample taken

15th Feb 2008)

Originally isolated at 1AH direct bovine blood

 

(28th Feb 2008)

Shelf A16,

box A16 β,

rows I1-J10

 

(Added to collection 

 16th Oct 2008)

 

[IAH/KC1/

BHK1]

 

Derived from

UKG2008/04

B520/08 1

 

UKG2008/32

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

 and sequence analyses (3)

From cattle

blood

 

Armoy, Ballymena,

Northern Ireland

 

Sample taken

13th Feb 2008

Originally isolated at 1AH-Pirbright

 

From cattle blood

(26th Feb 2008)

 

Animal

NL426303293

[IAH/KC1/

BHK1]

 

Derived from

UKG2008/06

 

The calf from this cow is represented by sample UKG2008/34

B492/08 1

 

UKG2008/31

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal  NL426303293

 

Armoy, Ballymena,

Northern Ireland

 

(14th Feb 2008)

Originally isolated at 1AH direct from spleen

 

(3rd March 2008)

Shelf A16,

box A16 β,

rows G1-H10

 

(Added to collection 

 16th Oct 2008)

 

[IAH/KC1/

BHK1]

Derived from

UKG2008/03

B513/08 1

 
UKG2008/30
 

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

 and sequence analyses (3) 

Animal

UK9171373884-4

1 week old calf

 

Armoy, Ballymena,

Northern Ireland

 

(Sample taken

16th Feb 2008)

Originally isolated at 1AH direct bovine blood

(28th Feb 2008)

Shelf A16,

Box 16 gamma,

rows A1 to B10

&

Shelf    D11

Box     D11β

Rows  I1-I6,

and rows  J1-J6

 

 (Added to

collection on

1st July 2014)

B522/08 1 [IAH/KC1/BHK1]

Derived from UKG2008/05

 

 
UKG2008/29

BTV-8

 

Identified by LSI real time BTV 8 assay

From cattle

blood  

Wales - UK

 

Sample taken  from a cow that was imported into Ruthin, in Wales  during late summer 2008, from France

Originally isolated at IAH-Pirbright, from cattle blood

 

(sampling date 

11th Sept 2008)

Shelf A14

Box A14 α

rows A1-B10

 

(Added to collection on

6th Oct 2008)

Animal No. FR2306751274

[IAH/E1/BHK2]

[Stars No.

 B1394/08 2]

 

UKG2008/28

BTV-8

 

Identified by LSI real time BTV 8 assay

 

From cattle

blood

 

England : UK

 

Sample taken  from a cow that was imported 4th Sept 2008 into Bromyard, England from central Germany

Originally isolated at IAH-Pirbright

 

From cattle blood

 

(sampling date 8TH Sept 2008)

Shelf A13

Box A13 GAMMA

I1-J10

Added 26/9/08

Animal No.  47804

[IAH/KC1]

[Stars No.

B1380/08 25]

single sample sample:

Misc  box, Freezer A,  Shelf E18, Box E18 α , position C12

 

UKG2008/27

BTV-8

 

Identified by LSI real time BTV 8 assay

 

From cattle

blood

 

England : UK

 

Sample taken  from a cow that was imported 4th Sept 2008

into Bromyard England from central Germany

 

Originally isolated at IAH-Pirbright

 

From cattle blood

 

(sampling date 8th Sept 2008)

Shelf A13,

box A13γ,

rows G1-H10

 

(Added to collection on 26th Sept 2008)

Animal No. 76720

[IAH/KC1]

 

[Stars No.

B1380/08 24]

 

Note only 9 vials of PDAM

single spare sample:

Misc  box, Freezer A, Shelf E18, Box E18 α ,  position C11

 

UKG2008/26

BTV-8

 

Identified by LSI real time BTV 8 assa

 

From cattle

blood

 

England : UK

 

Sample taken  from a cow that was imported 4th Sept 2008 into Bromyard, England from central Germany

Originally isolated at IAH-Pirbright

 

From cattle blood

 

(sampling date 8th Sept 2008)

Shelf A13,

Box A13 γ,

rows E1-F10

 

(Added to collection on 26th Sept 2008)

 

 

Animal No. 73991

[IAH/KC1]

[Stars No.

B1380/08 22]

 

 

Note only 9 vials of glycerol

single spare sample:

 Misc  box, Freezer A,  Shelf E18,ox E18 α , position C10

 

UKG2008/25

 

BTV-8

 

Identified by LSI real time BTV 8 assay

 

From cattle

blood

 

England: UK

 

Sample taken  from a cow that was imported 4th Sept 2008 into Bromyard, England from central Germany

Originally isolated at IAH-Pirbright 

from cattle  blood

 

(sampling date 8th Sept 2008)

Shelf A13,

Box A13 γ,

rows C1-D10

 

(Added to collection on 26th Sept 2008

Animal No.

21039

[IAH/KC1]

[Stars No.

B1380/08 20]

 

UKG2008/24

BTV-8

 

Identified by LSI real time BTV 8 assay

 

 

From cattle

blood

 

England

 

Sample taken  from a cow that was imported 4th Sept 2008 into Bromyard, England from central Germany

Originally isolated at IAH-Pirbright

 

From cattle  blood

 

(sampling date 8th Sept 2008)

Shelf A10,

Box A10β

rows I1-J10

(Added to collection on 26th Sept 2008

 

Animal No. 56314

[IAH/KC1]

[Stars No.

B1380/08 13]

 

UKG2008/23

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken  from a cow that was imported on 3rd Sept 2008 into South Petherton, England from central France

Originally isolated at IAH-Pirbright,

from cattle blood

 

(sampling date 5th Sept 2008)

Shelf A13

box A13γ,

rows A1-B10

 

Added to collection

24th Oct 2008

Animal id

FR3546493873

 

[IAH/KC1]

[Stars No.

 B1373/08 23]

 

UKG2008/22

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

England

 

Sample taken  from a cow that was imported on 6th Sept 2008 into Dorchester, Dorset, England from central France

Originally isolated at IAH-Pirbright from

cattle blood

 

(sampling date 6th Sept 2008)

 Shelf A13,

box A13β,

rows I1-J10

 

Added to collection

24th Oct 2008

 

 

Animal id

FR3533605702

 

[IAH/KC1]

 

[Stars No.

B1378/08 41]

 

 

UKG2008/21

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

 Sample taken  from a cow that was imported 4th Sept 2008 into Rushford (Newcastle) England, England from central Germany

Originally isolated at IAH-Pirbright from

cattle blood

 

(sampling date 6th Sept 2008)

 Shelf A13,

box A13β,

rows G1-H10

 

Added to collection

24th Oct 2008

Animal id

DE0350748125

[IAH/KC1]

[Stars No.

B1369/08 32]

 

UKG2008/20

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

 Sample taken  from a cow that was imported 4th Sept 2008 into Rushford (Newcastle) England, England from central Germany

Originally isolated at IAH-Pirbright from

cattle blood

 

(sampling date 6th Sept 2008)

 Shelf A13,

box A13β,

rows E1-F10

 

Added to collection

24th Oct 2008

Animal id

DE0348677283

[IAH/KC1]

[Stars No.

B1369/08 21]

 

UKG2008/19

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

 Sample taken  from a cow that was imported 4th Sept 2008 into Rushford (Newcastle) England, England from central Germany

Originally isolated at IAH-Pirbright from

cattle blood

 

(sampling date 6th Sept 2008)

 Shelf A13,

box A13β,

rows C1-D10

 

Added to collection

23rd Oct 2008

Animal id DE0348696174

[IAH/KC1]

[Stars No.

B13469/08 19]

 

UKG2008/18

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

 Sample taken  from a cow that was imported 4th Sept 2008 into Rushford (Newcastle) England, England from central Germany

ginally isolated at IAH-Pirbright from cattle blood

 

(sampling date 6th Sept 2008)

 Shelf A13,

box A13β,

rows A1-B10

 

Added to collection

23rd Oct 2008

Animal id DE0348677279

[IAH/KC1]

[Stars No.

B13469/08 10]

 

UKG2008/17

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

 Sample taken  from a cow that was imported 4th Sept 2008 into Rushford (Newcastle) England, England from central Germany

originally isolated at IAH-Pirbright from cattle blood

 

(sampling date 6th Sept 2008)

 Shelf A13,

box A13 α,

rows I1-J10

 

Added to collection

23rd Oct 2008

Animal id DE0348663886

[IAH/KC1]

[Stars No.

B13469/08 12]

Derived from blood sample UKG2008/52

UKG2008/16

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

 Sample taken  from a cow that was imported 4th Sept 2008 into Rushford (Newcastle) England, England from central Germany

originally isolated at IAH-Pirbright from cattle blood

 

(sampling date 6th Sept 2008)

 Shelf A13,

box A13 α,

rows G1-H10

 

 

Added to collection

23rd Oct 2008

Animal id DE0350330361

[IAH/KC1]

[Stars No.

B1369/08 13]

Derived from blood sample UKG2008/51

UKG2008/15

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken  from a cow imported on

19th Aug 2008

 into Tiverton England

from central Germany

Originally isolated at IAH-Pirbright from cattle blood

 

(sampling date 29th Aug 2008)

 Shelf A13,

box A13 α,

rows E1-F10

 

Added to collection

23rd Oct 2008

Animal Id DE0348929473

[IAH/KC2]

[Stars No.

B1346/08 33] 

Derived from blood sample UKG2008/50

UKG2008/14

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

 From sheep

blood

 

Sample taken  from a sheep that was imported into Hertfordshire

England on

19th Aug 2008,

from central France

Originally isolated at 1AH-Pirbright

 from sheep blood

 

(sampling date 29th Aug 2008)

Shelf A13,

box A13α,

rows C1-D10

 

Added to collection on 13th Oct 2008

No animal id,

south down sheep

[IAH/KC2]

[Stars No.

B1340/08 1]

Derived from blood sample UKG2008/49

UKG2008/13

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

 

(sampling date 29th Aug 2008)

 

Shelf A13,

box A13α

rows A1-B10

 

Added to collection on 13th Oct 2008

Animal id DE0348929478

[IAH/KC1]

[Stars No.

B1346/08 35]

Derived from blood sample UKG2008/48

UKG2008/12

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

 

 

(sampling date 29th Aug 2008)

Shelf A12,

box A12 γ,

rows I1-J10

 

Added to collection on 13th Oct 2008

Animal id DE0348682705

[IAH/KC1]

[Stars No.

B1346/08 30]

Derived from blood sample UKG2008/47

UKG2008/11

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbrightf rom cattle blood

 

(sampling date 29th Aug 2008)

 Shelf A12,

box A12γ ,

rows G1-H10

 

Added to collection on 13th Oct 2008

Animal id DE0350610251

[IAH/KC1]

[Stars No.

B1346/08 21]

note only 8 PDAM in freezer A and B)

 

Derived from blood sample UKG2008/46

UKG2008/10

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

 

(sampling date 29th Aug 2008)

Shelf A12,

box A12 γ,

rows E1-F10

 

Added to collection on 13th Oct 2008

Animal id DE0348684975

[IAH/KC1]

[Stars No.

B1346/08 20]

Derived from blood sample UKG2008/45

UKG2008/09

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

 

(sampling date 29th Aug 2008)

Shelf A12

box A12 γ,

rows C1-D10

 

Added to collection on 13th Oct 2008

Animal id DE0348986505

[IAH/KC1]

[Stars No.

B1346/08 17]

Derived from blood sample UKG2008/44

UKG2008/08

BTV-8

 

Identified by LSI BTV-8 specific  real time RT-PCR assay

From cattle

blood

 

Sample taken in England from a cow that was imported into Tiverton,  England on

19th Aug 2008, from central Germany

Originally isolated at 1AH-Pirbright from cattle blood

(sampling date 29th Aug 2008)

 Shelf A12,

box A12γ,

rows A1-B10

 

Added to collection on 13th Oct 2008

 

Animal Id DE0348986502

[IAH/KC1]

[Stars No.

B1346/08 16]

 

(note only 

9 vials PDAM

in freezer A and B)

Vial position A1 from Freezer A and given to Piers Wilkinson for sequencing on

28th February 2013

Corresponding vial in freezer B  has  not been removed

 

Derived from blood sample UKG2008/43

UKG2008/07

BTV-8

 

Identified by serotype specific real time RT-PCR assay (LSI)

From sheep

blood

 

Sample taken  from a ram that was imported on 19th Aug 2008 to Lewes in Sussex,  England,  from central France

Originally isolated at 1AH-Pirbright from sheep blood

 

(sampling date 20th Aug 2008)

 Shelf A12

box A12 β,

rows E1-F10

 

Added to collection on  5th Sept 2008

South Down Sheep

 

(no animal Id number)

[IAH/KC1]

[B1336/08 1 ]

Vial position E1 from Freezer A and given to Piers Wilkinson for sequencing on

28th February 2013

Corresponding vial in freezer B  has  not been removed
Derived from blood sample UKG2008/42

UKG2008/06

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3)

From cattle

blood

 

Armoy, Ballymena,

Northern Ireland

 

Sample taken

13th Feb 2008

Originally isolated at 1AH-Pirbright

 

From cattle blood

(26th Feb 2008)

Shelf A8

Box A8 γ

Rows  G1-H10

 

 (added to collection on

4th April 2008)

Animal

NL426303293

 

[IAH/KC1]

 

Used to generate UKG2008/32

 

The calf from this cow is represented by sample UKG2008/34

B492/08 1

Vial position G1 from Freezer A and given to Piers Wilkinson for sequencing on

28th February 2013 

UKG2008/05

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal

UK9171373884-4

1 week old calf

 

Armoy, Ballymena,

Northern Ireland

 

(Sample taken

16th Feb 2008)

Originally isolated at 1AH direct bovine blood

(28th Feb 2008)

Shelf A9,

Box A9 γ

Rows I1-J10

 

 (added to collection on

4th April 2008)

 

[IAH/KC1]

 

Used to 
generate

UKG2008/30

B522/08 1

*

Vial position J1 was removed from  Freezer B and given to Kyriaki Nomikou (Pirbright) on 19th February 2013.

Equivalent vial  in Freezer A was not removed

*

UKG2008/04

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2) 

 and sequence analyses (3) 

Animal

UK582544501188

 

Armoy, Ballymena,

Northern Ireland

 

(Sample taken

15th Feb 2008)

Originally isolated at 1AH direct bovine blood

 

(28th Feb 2008)

Shelf A9

Box A9 γ

Rows G1-H10

 

 (added to collection on

4th April 2008)

  [IAH/KC1]

 

Used to generate UKG2008/33

B520/08 1

*

Vial position H1 was removed from  Freezer B and given to Kyriaki Nomikou (Pirbright) on 19th February 2013.

Equivalent vial  in Freezer A was not removed

*

UKG2008/03

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal  NL426303293

 

Armoy, Ballymena,

Northern Ireland

 

(14th Feb 2008)

Originally isolated at 1AH direct from spleen

 

(3rd March 2008)

Shelf A9

Box A9 γ

Rows E1-F10

 

 (added to collection on

4th April 2008)

 

[IAH/KC1]

 

Used to generate

UKG2008/31

B513/08 1

 
UKG2008/02

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

 and sequence analyses (3) 

Animal

582544501188

 

Armoy, Ballymena,

Northern Ireland

 

Sample taken 18/2/08

Originally isolated at 1AH direct from spleen

 

(3rd March 2008)

Shelf A9

Box A9 γ

Rows C1-D10

 

 (added to collection on

4th April 2008)

  [IAH/KC1]

B534/08 3

 
UKG2008/01

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal

582544501188

 

Armoy, Ballymena,

Northern Ireland

 

Sample taken

18th Feb 2008

Originally isolated at 1AH direct from bovine blood

 

(3rd March 2008)

Shelf A9

Box A9 γ

Rows A1-B10

 

 (added to collection on

4th April 2008)

  [IAH/KC1]

B534/08 3

*
Duplicate vials  A10g  given to C. Batten (IAH) for ring trial
21st May 2010
*

Vial position B1 was removed from  Freezer B and given to Kyriaki Nomikou (Pirbright) on 19th February 2013.

Equivalent vial  in Freezer A was not removed

*

2007
( UK,     )
DEN2007/01

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2)

Sakskøbing, STORSTRØMS

Denmark

Sample taken 11th Oct 2007

From sheep blood

 

Isolated at

IAH-Pirbright

Shelf E6,

bov E6β,

rows I1-J10

 

(Added to collection on 20th May 2005)
ID1777 [IAH/KC1]

Original blood sample supplied by Anette Botner,

Lindholm

4771 Kalvehave Denmark

 

[A110/07]
Vial position I2 was   taken out of Freezer A and given o kyriaki Nomikou (Pirbright) 12th Jun 2013 (Corresponding vial in freezer B  has  not been removed)
FRA2007/17 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2007

Original tube marked as
dep 17 (6590)
BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal
FRA2007/16 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2007

Shelf D12,
Box D12 β,
rows A1 to B10
Original tube marked as 4119 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal

Only 19 vials in each freezer
FRA2007/15 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2007

  Original tube marked as 4065 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal
FRA2007/14 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2007

Shelf D12,

Box D12γ,

rows B1-B10

  

(Added to

collection on

23rd July 2014)

Original tube marked as 3719

E1BHK1

[BHK1]
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal 
FRA2007/13 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

 

in 2007

  Original tube marked as 3568 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal
FRA2007/12 BTV-8 from  France

isolated at AFSSA- Maisons Alfort France

in 2007

Shelf D12,
Box D12 α,
rows G1 to H10
Original tube marked as 3467 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal
FRA2007/11 BTV-8 from  France

2007

isolated at AFSSA- Maisons Alfort France

in 2007

Shelf A8,
box A8 α,
rows G1 to H10
Original tube marked as 3414 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal
FRA2007/10 BTV-8 from  France

2007

isolated at AFSSA- Maisons Alfort France

in 2007

Shelf A8,
 box A8 α,
rows C1 to D10
Original tube marked as 3405 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal
FRA2007/09 BTV-8 from  France

2007

isolated at AFSSA- Maisons Alfort France

in 2007

Shelf A8,
box A8 α,
rows A1 to B10
&
Shelf D12,
Box D12 α,
rows C1 to D10
Original tube marked as 3400 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal
FRA2007/08 BTV-8 from  France

2007

isolated at AFSSA- Maisons Alfort France

in 2007

Shelf A7,
box A7γ,
row I1 to J10
&
Shelf D12,
Box D12 α,
rows A1 to B10

(Added to

collection on

23rd July 2014)

Original tube marked as 3385 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal
FRA2007/07 BTV-8 from  France

2007

isolated at AFSSA- Maisons Alfort France

in 2007

Shelf A7 ,

box A7γ 

rows G1-H10

&

Shelf D12,

Box D12 γ,

rows A1 to A10

 

(Added to

collection on

23rd July 2014)

Original tube marked as 3303

E1BHK1

[BHK1]
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal 
FRA2007/06 BTV-8 from  France

2007

isolated at AFSSA- Maisons Alfort France

in 2007

Shelf A8,
box A8 α,
rows E1 to F10,
Original tube marked as 3138 BHK Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as BHK cell culture material
Outbreak data

from a non-
vaccinated animal

FRA2007/05

BTV-8

 

 (identified at Maisons Alfort, France)

North of Ardennes, France

 

(isolated at Maisons Alfort, France) 

 

Shelf E6,

Box E6α,

Rows G1-H10

 

 (Added to collection on  31st Oct 2007

3384

E1/BHK1

 

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

Outbreak data

FRA2007/04

BTV-8

 

 (identified at Maisons Alfort, France)

North of Ardennes, France

 

(isolated at Maisons Alfort, France) 

 

Shelf E5,

box E5γ,

rows  I1-J10

 (added to collection on 29th Oct 2007)

3414 E1BHK1

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

Outbreak data
FRA2007/03

BTV-8

 

 (identified at Maisons Alfort, France)

North of Ardennes, France

 

(isolated at Maisons Alfort, France)

 

Shelf E5,

box E5γ,

rows G1-H10

 

 (added to collection on 29th Oct 2007)
3400 E1BHK1

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

Outbreak data
FRA2007/02

BTV-8

 

 (identified at Maisons Alfort, France)

North of Ardennes, France

 

(isolated at Maisons Alfort, France) 

 

Shelf E5,

box E5γ,

rows E1-F10

 

 (added to collection on 29th Oct 2007)
3342 E1BHK1

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

Outbreak data
FRA2007/01

BTV-8

 

 (identified at Maisons Alfort, France)

North of Ardennes, France

 

(isolated at Maisons Alfort, France)

 

Shelf E5,

box E5γ,

rows C1-D10

 (added to collection on 29th Oct 2007)

3384 E1BHK1

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

Outbreak data

GER2007/26

BTV

Type 8

 

 

 

BTV-BH2/07-

[IAH/KC1/BHK1]

derived from

GER2007/24

 

GER2007/25

BTV

Type 8

   

Shelf A16,

box A16 β,

rows C1-D10

 

(Added to collection 

 16th Oct 2008)

BTV-BH30/07-

[IAH/KC1/BHK1]

derived from

GER2007/23

 

GER2007/24

BTV

Type 8

   

Shelf A15,

box A15 β,

rows E1-E6

and F1-F6

 

(Added to collection 

 16th Oct 2008)

BTV-BH2/07-

[IAH/KC1]

used to generate

GER2007/26

 

GER2007/23

BTV

Type 8

   

Shelf A15,

box A15 β,

rows A1-A7 and B1-B7

 

(Added to collection 

 16th Oct 2008)

BTV-BH30/07-

[IAH/KC1]

used to generate GER2007/25

 
 

GER2007/22

BTV-8

 

Identified as BTV by

RT-PCR

 

 (by BTV-8 specific real time RT-PCR on blood sample, in Germany)

Cattle blood,

 from Thuringia, Germany

 

Sample

collected

3rd Sept2007

 

Cattle blood

Shelf  A7,

box A7 γ,   

rows E1-F10

 

Added to collection

20th Dec 2007

Animal No.

BH191/07 3

From cattle blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

 

GER2007/21

BTV-8

 

Identified as BTV by

RT-PCR

 

 (by BTV-8 specific real time RT-PCR on blood sample, in Germany)

Cattle blood,

 from Thuringia, Germany

 

Sample

collected

3rd Sept2007

Cattle blood

Shelf  A7,

box A7 γ,   

rows C1-D10

 

Added to collection

20th Dec 2007

Animal No.

BH191/07 2

From cattle blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

GER2007/20

BTV-8

 

Identified as BTV by

RT-PCR

 

 (by BTV-8 specific real time RT-PCR on blood sample, in Germany)

Cattle blood,

 from Thuringia, Germany

 

Sample

collected

3rd Sept2007

Cattle blood

Shelf  A7,

box A7 γ,   

rows A1-B10

 

Added to collection

20th Dec 2007

 

 

Animal No. BH191/07 1

From cattle blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

 

GER2007/19

BTV-8

 

Identified as BTV by

RT-PCR

 

  (by BTV-8 specific real time RT-PCR on blood sample, in Germany)

Cattle blood, from  Bavaria, Germany

 

Sample

collected

1st Sept2007

Cattle blood

Shelf  A7,

box A7β,

rows I1-J10

 

Added to collection

20th Dec 2007

 

Animal No.

BH188/07 2

From cattle blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

GER2007/18

BTV-8

 

Identified as BTV by

RT-PCR

 

 (by BTV-8 specific real time RT-PCR on blood sample, in Germany)

Cattle blood, from  Bavaria, Germany

 

Sample

collected

1st Sept2007

Cattle blood

Shelf  A7.

box A7β,   

rowsG1-H10

 

Added to collection

20th Dec 2007

Animal No. BH188/07

From cattle blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

GER2007/17

BTV-8

 

Identified as BTV by

RT-PCR

 

  (by BTV-8 specific real time RT-PCR on blood sample, in Germany)

Sheep Blood, from  Lower Saxony, Germany

 

Sample

collected

 31st Aug 2007

Sheep blood

Shelf  A7,

box A7β,    

rows E1-F10

 

Added to collection

20th Dec 2007

Animal No. BH183/07 1

From Sheep blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

GER2007/16

BTV-8

 

BTV by Seg-1 RT-PCR at

IAH-P (1)

 

(identified by real time RT-PCR targeting Seg-5 (NS1) of BTV-8, on blood sample, at FLI Riems)

Cattle blood,

 from

Lower Saxony,

Germany

 

Sample

collected

30th  Aug 2007

From cattle blood

 

(isolated at IAH, November 2007)

Shelf A7,

box A7 β,

rows C1-D10

 

Added to collection

20th Dec 2007

Animal No.

BH179/07 2

 

From cattle blood

 

[IAH/KC1]

 

Bernd Hoffmann

(FLI Riems, Germany)

 

Arrived at IAH 3rd Oct 2007

 

GER2007/15

BTV-8

 

BTV by Seg-1 RT-PCR at

IAH-P  (1)

 

(identified by real time RT-PCR targeting Seg-5 (NS1) of BTV-8, on blood sample, at FLI Riems)

Saarland, Germany

 

Sample

collected

30th  Aug 2007

From sheep blood

 

(isolated at IAH, November 2007)

Shelf  A7,

 box A7α ,

rows I1-J10

 

Added to collection

14th Dec 2007

Animal No.

BH178/07 5

From sheep blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

Arrived at IAH 3rd Oct 2007

 

GER2007/14

BTV-8

 

BTV by Seg-1 RT-PCR at

IAH-P (1)

 

(identified by real time RT-PCR targeting Seg-5 (NS1) of BTV-8, on blood sample, at FLI Riems)

Saarland, Germany

 

Sample

collected

30th  Aug 2007

From cattle blood

 

(isolated at IAH, November 2007)

Shelf A7,

box A7α 

rows G1-H10

 

Added to collection

14th Dec 2007

Animal No.

BH178/07 2

 

From cattle blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

Arrived at IAH 3rd Oct 2007

 

GER2007/13

BTV-8

BTV by Seg-1 RT-PCR at

IAH-P (1)

(identified by real time RT-PCR targeting Seg-5 (NS1) of BTV-8, on blood sample, at FLI Riems)

Saarland, Germany

Sample

collected

30th  Aug 2007

From cattle blood

(isolated at IAH, November 2007)

Shelf  A7,

box A7α,

rows E1-F10

Added to collection

7th Dec 2007

Animal No.

BH178/07 1

From cattle blood

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

Arrived at IAH 3rd Oct 2007

GER2007/12

BTV-8

BTV by Seg-1 RT-PCR at

IAH-P (1)

(identified by real time RT-PCR targeting Seg-5 (NS1) of BTV-8, on blood sample, at FLI Riems)

North-Rhine Westphalia,

Germany

Sample

collected

23rd Aug 2007

From cattle blood

(isolated at IAH, November 2007)

Shelf A7,

box A7α, 

rows C1-D10

Added to collection

14th Dec 2007

Animal No.

BH170/07 3

From cattle blood

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

Arrived at IAH 3rd Oct 2007

GER2007/11

BTV-8

 

BTV by Seg-1 RT-PCR at

IAH-P  (1)

 

(identified by real time RT-PCR targeting Seg-5 (NS1) of BTV-8, on blood sample, at FLI Riems)

North-Rhine Westphalia,

Germany

 

Sample

collected

 23rd Aug 2007

From cattle blood

 

(isolated at IAH, November 2007)

Shelf  A7,

box A7 α, 

Rows A1-B10

 

Added to collection

7th Dec 2007

Animal No.

BH170/07 2

From cattle blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

Arrived at IAH 3rd Oct 2007

 

GER2007/10

BTV-8

 

BTV by Seg-1 RT-PCR at

IAH-P  (1)

 

(identified by real time RT-PCR targeting Seg-5 (NS1) of BTV-8, on blood sample, at FLI Riems)

North-Rhine Westphalia,

Germany

 

Sample

collected

23rd Aug 2007

From sheep

 blood

 

(isolated at IAH, November 2007)

Shelf E6,

box E6 γ ,   

rows I1-J10

 

Added to collection

7th Dec 2007

Animal No.

BH169/07 41

From sheep blood

 

[IAH/KC1]

 

Bernd Hoffmann

(FLI Riems, Germany)

 

Arrived at IAH 3rd Oct 2007

 

GER2007/09

BTV-8

 

BTV by Seg-1 RT-PCR at

IAH-P  (1)

 

(identified by real time RT-PCR targeting Seg-5 (NS1) of BTV-8, on blood sample, at FLI Riems)

North-Rhine Westphalia,

Germany

 

Sample

collected

23rd Aug 2007

From sheep blood

 

(isolated at IAH, November 2007)

Shelf E6,

box E6γ,    

rows G1-H10

 

Added to collection

7th Dec 2007

Animal No. BH169/07 8

From sheep blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems, Germany)

 

Arrived at IAH 3rd Oct 2007

 

GER2007/08

BTV-8

 

BTV by Seg-1 RT-PCR at

IAH-P  (1)

 

(identified by real time RT-PCR targeting Seg-5 (NS1) of BTV-8, on blood sample, at FLI Riems)

North-Rhine Westphalia,

Germany

 

Sample

collected

23rd Aug 2007

From cattle blood

 

(isolated at IAH, November 2007)

Shelf E6,

  box  E6 γ, 

rows E1-F10

 

Added to collection

7th Dec 2007

Animal No. BH170/07 4

From cattle blood

 

[IAH/KC2]

Bernd Hoffmann

(FLI Riems, Germany)

 

Arrived at IAH 3rd Oct 2007

 

GER2007/07

 

BTV-8

 

BTV by RT-PCR

 

(Blood BTV-8 by BTV-8 specific real time PCR in Germany)

Germany,

North-Rine Westphalia

 

Sample taken

13th Sept 2007

Positive  cattle blood

animal No BH207/07 14

 Shelf E5,

box E5 α,

rows C1-D10

 

Added to collection on  12th Oct 2007

ANIMAL No BH207/07 14

[IAH/E1/BHK 2]

Bernd Hoffmann

(FLI Riems)

 

GER2007/06

 

identified as BTV by

RT-PCR

 at IAH-Pirbright

 

 identified  as BTV-8

by BTV 8 specific real time PCR in Germany

North-Rine Westphalia

 

Sample collected

13th Sept 2007

Seropositive

sheep blood

from the field

 Shelf E4,

 box E4 β,

rows A1-B9

 

Added to collection on  5th Oct 2007

Animal No BH207/07 13

From Sheep blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems)

 

NOTE only 9 vials available in pdam

Sample A1 given to Anna Swain IAH on 16th May 2008 (Duplicate tube left in Shelf E1, Box E1β)

GER2007/05

BTV by

RT-PCR,

 at IAH-Pirbright

 

 identified  as BTV-8

by BTV 8 specific real time PCR in Germany

FLI-Isle of Riems

 

From an experimental infection

19th Sept 2007

Seropositive

cattle blood

Shelf E4,

box E4 β,

rows C1-D9

 

Added to collection on  5th Oct 2007

Animal  No BHV06/07 597

 

From cattle blood

 

[IAH/KC1]

Bernd Hoffmann

(FLI Riems)

 

NOTE only 9 vials available in glycerol

 

GER2007/04

BTV-8

 

typed as BTV 8 by Seg-2  specific

RT-PCR in Germany

Hückeswagen near the German town of "Wupperthal" (Northrhine-Westfalen)

Mixed sample taken on the 2nd and 21st April 2007

Positive cattle blood from the field  was used to infect animal no 415

 

Blood from 415 was used to inoculate

*ETH-cells

in Riems

Shelf E1,

 box E1γ,

rows G1-H10

 

added to collection

6th July 2007

ANIMAL No 415 9dpi

*ETH1

'BHK-C13'1

[IAH BHK1]

 

* ETH=

aorta-endothelial-cell line

Bernd Hoffmann

(FLI Riems)

from the same animal as GER2007/02

GER2007/03

BTV-8

 

typed as BTV 8 by Seg-2  specific

RT-PCR in Germany

Hückeswagen near the German town of "Wupperthal" (Northrhine-Westfalen)

 

Mixed sample taken on the 2nd and 21st April 2007

Positive cattle blood from the field  was used to infect animal (cattle)  no 418

 

Blood from 418 was used to inoculate

*ETH-cells

in Riems

Shelf E1,

 box E1 γ,

 rows C1-D10

 

added to collection

13th July 2007

ANIMAL No 418 9dpi

*ETH1

'BHK-C13'1

[IAH BHK1]

 

* ETH=

aorta-endothelial-cell line

Bernd Hoffmann

(FLI Riems)

from the same animal as

GER2007/01

GER2007/02

BTV-8

 

typed as BTV 8 by Seg-2  specific

RT-PCR in Germany

Hückeswagen near the German town of  "Wupperthal" (Northrhine-Westfalen)

 

Mixed sample taken on the 2nd and 21st April 2007

Positive cattle blood from the field  was used to infect animal (cattle) no 415.

 

Blood from 415 was used to inoculate

*ETH-cells

in Riems

Shelf E1,

 box E1γ,

rows E1-F10

 

added to collection

13th July 2007

ANIMAL No 415 6dpi

*ETH1

'BHK-C13'1

[IAH BHK1]

 

* ETH=

aorta-endothelial-cell line

Bernd Hoffmann

(FLI Riems)

from the same animal as GER2007/04

GER2007/01

 

BTV-8

 

typed as BTV 8 by Seg-2  specific

RT-PCR in Germany

Hückeswagen near the German town of  "Wupperthal" (Northrhine-Westfalen)

 

Mixed sample taken on the 2nd and 21st April 2007

Positive cattle blood from the field  was used to infect animal no 418

 

Blood from 418 was used to inoculate

*ETH-cells

in Riems

Shelf E1,

 box E1γ,

rows A1-B10

 

added to collection

6th July 2007

ANIMAL No 418 6 dpi

*ETH1

'BHK-C13'1

[IAH BHK1]

 

* ETH=

aorta-endothelial-cell line

Bernd Hoffmann

(FLI Riems)

(note B10 in freezer B <1ml)

 

from the same animal as GER2007/03

 

 Single sample,  - E1 γ position A1(G), supplied to Anna Swain 16th May 2008, IAH Pirbright

(second vial kept as misc)

NET2007/09

BTV

Type 8

 

Someren,

the Netherlands

 

Blood sample from a goat animal No: 6126

 

17th Sept 2007

From EDTA treated goat blood

 

(Isolated at 

IAH Pirbright)

Shelf E5,

box E5 α,

rows A1- B10

 

(added to collection on 12th Oct 2007 )

ANIMAL NO:6126

[IAH/E1/BHK2]

Sample supplied by Piet van Rijn CIDC Lelystad

 

[A94/07 4]

Outbreak data

NET2007/08

BTV- 8

Someren,

the Netherlands

 

Blood sample collected

from animal

7317

 

17th Sept 2007

From EDTA treated goat blood

 

(Isolated at  IAH Pirbright

Sept-Oct 2007)

Shelf E4,

box E4 β,

rows E1- F10

 

(added to collection on

5th Oct 2007 )

 

from goat

 blood

 

[IAH/E1/BHK2]

Piet van Rijn CIDC Lelystad

 

A94/07 03

 From same animal as NET2007/07

Outbreak data

NET2007/07

 

BTV

Type 8

Someren,

the Netherlands

 

Blood sample collected

from animal

7317

 

17th Sept 2007

From EDTA treated goat blood

 

(Isolated at  IAH Pirbright

Sept-Oct 2007)

Shelf E4,

box E4 BETA,

rows I1- J10

 

(added to collection on

5th Oct 2007 )

 

from goat

 blood

 

[IAH/KC1]

Piet van Rijn CIDC Lelystad

 

A94/07 03

From same animal as

NET2007/08

 Single sample,  - E1 γ position A1(G), supplied to Anna Swain 16th May 2008,

IAH Pirbright (second vial kept as misc)

 Outbreak data

NET2007/06

BTV

Type 8

Someren,

the Netherlands

 

Blood sample collected  from animal  6126

 

17th Sept 2007

From EDTA treated goat blood

 

(Isolated at the CRL, IAH Pirbright

Sept-Oct 2007)

Shelf E4,

box E4 GAMMA

rows E1- F10

 

(added to collection on

6th Oct 2007 )

 

from goat

 blood

 

[IAH/KC1]

Piet van Rijn CIDC Lelystad

 

A94/07 04

Outbreak data

NET2007/05

 

BTV

Type 8

Near Nuth, Limburg, The Netherlands.

 

Blood sample taken

30th Aug 2007

From EDTA treated sheep  blood

 

(Isolated at CRL)

Shelf E3,

box E3γ,

rows G1- H10 

 

added to collection on

26th Sept 2007

Animal Number

X07023351-001 1243828

From sheep

 blood

 

[IAH/E1 BHK1

Piet Van Rijn CIDC

Lelystad

 

(from same sample as NET2007/03 )

Outbreak data

NET2007/04

BTV

Type 8

 

Elsloo,

Limburg, The Netherlands.

 

Sample taken

30th Aug 2007

From EDTA  treated bovine blood

 

(Isolated at CRL)

Shelf E3,

box E3γ,

rows I1- J10

 

added to collection on

26th Sept 2007

Animal Number

X07023352-001 NL394260253

From sheep

 cattle

 

[IAH/E1 BHK1]

Piet Van Rijn CIDC

Lelystad

 

(from same sample as NET2007/02)

Outbreak data

NET2007/03

 

BTV

Type 8

Near Nuth, Limburg, The Netherlands.

 

Blood sample taken

30th Aug 2007

From EDTA treated sheep  blood

 

(Isolated at CRL, IAH Pirbright)

Shelf E3,

box E3γ,

rows C1- D10

 

 added to collection on

26th Sept 2007

Animal number

X07023351-001 1243828

From sheep

 blood

 

[IAH/KC1]

Piet Van Rijn CIDC

Lelystad

 

(from same sample as NET2007/05)

Outbreak data

NET2007/02

BTV

Type 8

Elsloo,

Limburg, the Netherlands.

 

blood sample collected

30th August 2007

From bovine EDTA blood

 

(Isolated at CRL, IAH Pirbright)

Shelf E3,

box E3γ,

rows A1- B10

 

added to collection

26th Sept 2007

Animal Number

X07023352-001 NL394260253

 

[IAH/KC1]

Piet Van Rijn, CIDC,

 Lelystad

(from same sample as NET2007/04)

Outbreak data

NET2007/01

 

 

BTV

Type 8

 

 (Typed by

Seg-2  RT-PCR  and  sequence analysis)

Bavel,

Noord Brabant, the Netherlands.

 

(sample taken

27th July 2007 )

From bovine EDTA blood

 

(Isolated at IAH Pirbright

August 2007)

Shelf E1,

box E1γ,

rows I1- J10

 

(added to collection on 30th Aug 2007

Bavel July 27

Animal Number NL441689187

From Blood

 

[IAH/E1/ BHK2]

Blood sample supplied by Hans Kramps,

 CIDC Lelystad

The Netherlands 

 

[A75/07]

Outbreak data

[Seg-1: GQ506451,

 Seg-2: GQ506452,

Seg-3: GQ506453,

Seg-4: GQ506454,

Seg-5: GQ506455,

Seg-6:  GQ506456,

Seg-7: GQ506457,

Seg-8: GQ506458,

Seg-9: GQ506459,

Seg-10: GQ506460]

 

SWI2007/01

BTV 8

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

Basel, Switzerland

 

sample taken  7th Nov 2007

from cattle blood

into KC cells

 

Isolated at

IAH-Pirbright

Shelf A7

box A7 β

rows A1-B10

 

(Added to collection on  20th Dec 2007)

 

[IAH/KC2]

Barbara Thur,

Institut fur viruskrankheiten und immunprophylaxe, sensemattstr. 293, 3175 mittlehausern, switzerland

 

[A139/07 3]

 

UK-2007
UKG2007/98 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row F1 to F5
       
UKG2007/97 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row E6 to E10
       
UKG2007/96 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row E1 to E5
       
UKG2007/95 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row D6 to D10
       
UKG2007/94 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row D1 to D5
       
UKG2007/93 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row C6 to C10
       
UKG2007/92 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row C1 to C5
       
UKG2007/91 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row B6 to B10
       
UKG2007/90 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row B1 to B5
       
UKG2007/89 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row A6 to A10
       
UKG2007/88 BTV 8 UK
2007
  Shelf D14,
Box D14 gamma,
row A1 to A5
       
UKG2007/87 BTV 8 UK
2007
  Shelf D14,
Box D14 β,
row J6 to J10
       
UKG2007/86 BTV 8 UK
2007
  Shelf D14,
Box D14 β,
row J1 to J5
       
UKG2007/85 BTV 8 UK
2007
  Shelf D14,
Box D14 β,
row I6 to I10
       
UKG2007/84 BTV 8 UK
2007
  Shelf D14,
Box D14 β,
row I1 to I5
       

UKG2007/83

BTV-8

Identified by serotype specific (Seg-2)

RT-PCR (2)

and sequence analyses (3

isolated from an original blood sample collected from a rare breeds farm, Baylham house , Baylham, Ipswich, Suffolk

UK

(blood sample collected

21st Sept 2007)

Virus isolated from washed and sonicated EDTA treated cattle blood, applied directly to KC cells

Shelf D9,
box D9 alpha,
rows I1 to J10


added to collectionion
13th Dec 2014

UK index case BTR2007/24

from the highland cow called “Debbie”

[IAH/

KC2]

see also UKG2007/82

Outbreak Data

 

*

Derived from UKG2007/06

*

Prepared on 13th of  February 2012 from UKG2007/06 and used for oral infection of sheep during  animal experiments done by Karin Darpel

UKG2007/82

BTV-8

Identified by serotype specific (Seg-2)

RT-PCR (2)

and sequence analyses (3

isolated from an original blood sample collected from a rare breeds farm, Baylham house , Baylham, Ipswich, Suffolk

UK

(blood sample collected

21st Sept 2007)

Virus isolated from washed and sonicated EDTA treated cattle blood, applied directly to KC cells

Shelf D9,
box D9 alpha,
rows G1 to H10


added to collectionion
13th Dec 2014

UK index case BTR2007/24

from the highland cow called “Debbie”

[IAH/

KC2]

see also UKG2007/83

Outbreak Data

*

Derived from UKG2007/06

*

 Prepared on 27th of January  2012 from UKG2007/06 and used for oral infection of sheep  by Karin Darpel

UKG2007/81 BTV 8 2007 Virus isolate Shelf D9,
box D9 alpha,
rows E1 to F10

added to collectionion 2014
       
UKG2007/80 BTV 8 2007 Virus isolate Shelf D8,
box D8 γ,
rows C1 to D10

added to collectionion 2014
       
UKG2007/79 BTV 8 2007 Virus isolate Shelf D8,
box D8 γ,
rows A1 to B10

added to collectionion 2014
note: only 19 in each freezer
UKG2007/78
(virus isolate)
               
UKG2007/78
 (blood  sample)

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2)

Essex, UK

(OS ref TL8222707858)

 

Sample taken

4th Oct 2007

Cattle blood, from

animal number UKS501800113

 

Isolated at

IAH-Pirbright

Shelf B16

Box B16 gamma

tubes I2 & J2

 

(Added to collection on  1st Nov 2007)

BTR2007/98

Blood sample

B356/07-1

Used to generate isolate UKG2007/15

Only two tubes in freezer A
UKG2007/77

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

 and sequence analyses (3)

Original sample collected from a rare breeds farm, Baylham house , Baylham, Ipswich,  Suffolk

UK

 

(blood sample collected

21st Sept 2007)

Virus isolated from washed and sonicated EDTA treated cattle blood, applied directly to KC cells.

(isolated on ??)

Shelf C18

Box C18β

Rows E1-F10

 

(Added to

collection on

1st June 2012)

UK index case BTR2007/24

 

from the highland cow called “Debbie”
[IAH/KC3] B446/07-195

Outbreak Data

Derived from UKG2007/64

*

100ml of this passage level sent to Piet van Rijn CVI at Lelystad the Netherlands, as a vaccine

challenge strain

*

100ml of this passage level sent to Stephan Zientara ANSES at Maisons Alfort France, as a vaccine challenge strain on 17th Apr 2012

Moulin et al 2012 [5]
UKG2007/76 BTV-8 (blood)

Shelf B16,

box B16 g,

rows I1 and J1

 

Added to collection on

(25th March 2010) 

Only 1 vial (P) for freezer A (I1) and 1 vial (G) for freezer A (J1)

UKG2007/75

BTV-8 (blood)

Shelf B16,

box B16 g,

rows I2 and J2

 

Added to collection on

(25th March 2010)

 

Only 1 vial (P) for freezer A (I2) and 1 vial (G) for freezer A (J2)

UKG2007/74 BTV-8 (blood)  

Shelf B16,

box B16 b,

rows F1

 

(Added to collection on 25th Mar 2010)

only 1 vials (G) for each freezer

UKG2007/73

BTV-8 (blood)

 

Shelf B16,

box B16 β,

rows C7 –C8 

 

Added to collection on 25th Mar 2010 

only 2 vials (G)
or each freezer
UKG2007/72 BTV-8 (blood)

Shelf B16,

box B16 β,

rows E5 –E6

 

(Added to collection on 25th Mar 2010)

only 2 vials (G) for each freezer

UKG2007/71

BTV-8 (blood)

   

Shelf B16,

box B16 β,

rows E8 –E10

 

(Added to collection on 25th Mar 2010)

only 3 vials (G) for freezer A (E8-E10) and only 2 vials (G) for freezer B (E8-E9)

UKG2007/70 BTV-8 (blood)  

Shelf B16,

box B16 b,

rows F3-F4

 

Added to collection on

(25th March 2010)    

only 2 vials (G) for each freezer

UKG2007/69

BTV-8 (blood)

 

Shelf B16,

box B16 b,

rows F6-F8

 

Added to collection on

(25th March 2010)    

only 3 vials (G) for freezer A (F6-F8) and only 4 vials (G) for freezer B (F6-F9)

UKG2007/68

BTV-8 (blood)

 

Shelf B16,

box B16 b,

rows G1-G9

 

Added to collection on

(25th March 2010)    

only 9 vials (G) for freezer A (G1-G9) and only 10 vials (G) for freezer B (G1-G10)

UKG2007/67 BTV-8 (blood)  

Shelf B16,

box B16 b,

rows H3

 

Added to collection on

(25th March 2010)    

B446/07-199

only 1 vial (G) for freezer A

UKG2007/66

BTV-8 (blood)

 

Shelf B16,

box B16 b,

rows H4

 

Added to collection on

(25th March 2010)    

B446/07-198

only 1 vials (G) for freezer A

UKG2007/65 BTV-8 (blood)    

Shelf B16,

box B16 b,

rows I1-J8

    B446/07-196 21 vials in freezer B (H6-J6) ;
18 in freezer A (I1-J8)

UKG2007/64

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

 and sequence analyses (3)

Original sample collected from a rare breeds farm, Baylham house , Baylham, Ipswich,  Suffolk

UK

 

(blood sample collected

21st Sept 2007)

Virus isolated from washed and sonicated EDTA treated cattle blood, applied directly to KC cells.

(isolated on ??)

??

UK index case BTR2007/24

 

from the highland cow called “Debbie”

[IAH/KC2]

B446/07-195

Outbreak Data

Derived from UKG2007/06

Used to generate UKG2007/77

*

Supplied to Intervet (Boxmeer) for vaccine trial in sheep  (10th Apr 2008)

*

Moulin et al 2012 [5]
UKG2007/63

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

 and sequence analyses (3) 

Pidgeon Hall Farm

Ipswich

EDTA Blood from ill neonatal calf. Died three days old

 

(Virus isolated at IAH –P on

20th Feb 2008)

 Shelf A9,

box A9 β,

rows A1-B10

 

 (added to collection on 13th March 2008)

TR2007/117 [IAH/KC1]

Aho

Bury St Edmunds

 

B541/07 1
Dam UK224093300002 was seropositive for BTV

 

UKG2007/62  

Colchester

 

Shelf B16,

box B16 g,

rows F1-G7

 

Added to collection on

(25th March 2010)

    B412/07-1 Only 6 vials (P) for freezer A (F1-F6); 5 vials (P) for freezer B (F1-F5);  7 vials (G) for freezer A (G1-G7) and  6 vials (G) for freezer B (G1-G6)

Outbreak Data
UKG2007/61  

Colchester

(ovine)

Shelf B16,

box B16 g,

rows D1-E7

 

Added to collection on

(25th March 2010)

    B402/07-1

Only 7 vials (P) for freezer A (D1-D7); 6 vials (P) for freezer B (D1-D6);  7 vials (G) each for freezer A and B (E1-E7)

 

Outbreak Data

UKG2007/60

Shrubland, Coddenham

Shelf B16,

box B16 g,

rows C1-C10

 

Added to collection on

(25th March 2010)

Blood cells washed in PBS [B259/07 20] Only 5 vials (P) for freezer A  and B (C1-C5); 4 vials (G) for freezer A and B (C7-C10)


Outbreak Data
UKG2007/59

BTV-8

Maylane, Lowestoft Suffolk

 

Shelf B16,

box B16 gamma,

rows B1-B8

 

Added to collection on

(25th March 2010)    

  Blood cells washed in PBS [B251/07 1] Only 4 vials (P) for freezer A (B1-B4); 3 vials (P) for freezer B (B1-B3);  3 vials (G) for freezer A (B6-B8) and 2 vials (G) for freezer B (B6-B7)



[S2, S5, S7]

Outbreak Data

UKG2007/58   Tunbridge
Wells
 

Shelf B16,

box B16 gamma,

rows A5-A9

 

Added to collection on

(25th March 2010)

    B446/07-203 Only 3 vials (P) for freezer A and B (A1-A3); 5 vials (G) for freezer A (A5-A9) and 4 vials (G) for freezer B (A5-A8

Outbreak Data
UKG2007/57  

Leavenheath, Suffolk

 

Shelf B17,

box B17 α,

rows G2 and H2

 

Added to collection on

(25th March 2010)    

  Blood cells washed in PBS [B539/07 1] 1 vial (P) each for freezer A and B (G3)   and only 1 vial (G) for freezer A (H2)

Outbreak Data
UKG2007/56   Newton, Sudbury

Shelf B17,

box B17 α,

rows G1 and H1

 

Added to collection on

(25th March 2010    

Blood cells washed in PBS [B537/07 1] 1 vial (P) each for freezer A and B (E10)   and 1 vial (G) each for freezer A and B (F10)

Outbreak Data
UKG2007/55  

Nayland, Colchester

 

Shelf B17,

box B17 α,

rows E10 and F10

 

Added to collection on

(25th March 2010)    

  Blood cells washed in PBS [B536/07 1] 1 vial (P) for freezer A (E10)   and 1 vial (G) for freezer A (F10)

Outbreak Data
UKG2007/54  

Nayland, Colchester

 

Shelf B17,

box B17 α,

rows E9 and F9

 

Added to collection on(25th Mar 2010)

Blood cells washed in PBS [B536/07 2] 1 vial (P) for freezer A (E9)   and 1 vial (G) for freezer A (F9)

Outbreak Data
UKG2007/53

Haddenham, Cambridgeshire

Shelf B16,

box B16 g,

rows H1-H5

 

Added to collection on

(25th March 2010)

Blood cells washed in PBS [B534/07 1] Only 2 vials (G) each for freezer A and B (H1-H2) and  3 vials (P) for freezer B (H3-H5)

Outbreak Data
UKG2007/52  

Woodlane, Oulton, Lowestoft

Shelf B17,

box B17 α,

rows E7 and F7

 

Added to collection on

(25th March 2010)    

  Blood cells washed in PBS [B460/07 1] 1 vial (P) each for freezer A and B (E7)   and 1 vial (G) for freezer A (F7)

[S2]

Outbreak Data

UKG2007/51  

Stonestreet, Boxford

 

Shelf B17,

box B17 α,

rows E6

 

Added to collection on

(25th March 2010)    

Blood cells washed in PBS [B425/07 1] 1 vial (P) each for freezer A and B (E6) 

[S2, S7]

Outbreak Data

UKG2007/50  

Tentender, Kent

 

Shelf B17,

box B17 α,

rows E5

 

Added to collection on

(25th March 2010)    

  Blood cells washed in PBS [B443/07 1] 1 vial (P) for freezer A (E5)
 
Outbreak Data
UKG2007/49

Sevenoaks

Shelf B17,

box B17 α,

rows E2-E4 and F2-F3

 

Added to collection on

(25th March 2010)    

Blood cells washed in PBS B463/07-01 [Sevenoaks] 3 vials (P) for freezer A (E2-E4); 2 vials (P) for freezer B (E2-E3); 2 vials (G) each for freezer A and B (F2-F3)

[S2, S7]

Outbreak Data

UKG2007/47           Blood cells washed in PBS [B252/07-15 [UKG2007/04 = Lorraine]] [S2, S7]

Outbreak Data

UKG2007/46           Blood cells washed in PBS [B426/07-1 [UKG2007/05 =Debbie]] [S2, S7]

Outbreak Data

UKG2007/45

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

Peterborough, UK

(OS ref TF153008)

 

Sample taken 14th Oct 2007

Sheep blood, from animal number UK223943000490

 

Isolated at

IAH-Pirbright

Shelf B16

Box B16 gamma

Rows I1 & J1

 

(Added to collection on  1st Nov 2007)

BTR2007/150

Blood sample

[B411/07] [S2]

Used to generate isolate UKG2007/14

UKG2007/44  

Topcroft, Bungay

 

Shelf B17,

box B17 α,

rows E1 and F1

 

Added to collection on

(25th March 2010)    

  Blood cells washed in PBS B419/07 1 1 vial (P) each for freezer A and B (E1) and only 1 vial (G)  freezer A (F1) 

[S2]

Outbreak Data

UKG2007/43  

Ashford, Kent

 

Shelf B17,

box B17 α,

rows C10 and D10

 

Added to collection on

(25th March 2010)    

  Blood cells washed in PBS [B418/07 1] Only 1 vial (P) for freezer A (C10) and 1 vial (G) for freezer A (D10) 

[S2]

Outbreak Data

UKG2007/42

     

Shelf B17,

box B17 α,

rows C9 and D9

 

Added to collection on

(25th March 2010)    

 

Blood cells washed in PBS

[B377/07 4]

1 vial (P) each for freezer A and B (C9) and 1 vial (G) each for freezer A and B (D9) 

 

[S2]

 

Outbreak Data

UKG2007/41

     

Shelf B17,

box B17 α,

rows C8 and D8

 

Added to collection on

(25th March 2010)    

 

Blood cells washed in PBS

[B377/07 3]

Only 1 vial (P) for freezer A (C8) and 1 vial (G) for freezer A (D8) 

 

Outbreak Data

UKG2007/40

Shelf B17,

box B17 α,

rows C7 &  D7

 

Added to collection on

(25th March 2010)    

Blood cells washed in PBS [B377/07 2] Only 1 vial (P) for freezer A (C7)
and 1 vial (G) for freezer A (D7)


Outbreak Data
UKG2007/39   Lound, Lowestoft  

Shelf B17,

box B17 α,

rows C6 and D6

 

Added to collection on

(25th March 2010)    

  Blood cells washed in PBS [B377/07 1] Only 1 vial (P) for freezer A (C6) and 1 vial (G) for freezer A (D6) 


Outbreak Data
UKG2007/38   Purleigh, Essex

Shelf B17,

box B17 alpha,

rows C5 & D5

 

Added to collection on

(25th Mar 2010)

Blood cells washed in PBS [B370/07 1] Only 1 vial (P) for freezer A (C5) and 1 vial (G) for freezer A (D5)

[S2]

Outbreak Data

UKG2007/37

 

Blakenham Farms,

Ipswich,  UK

 

Shelf B17,

box B17 alpha,

rows C4

 

Added to collection on

(25th Mar 2010)

 

Blood cells washed in PBS

[B262/07 23]

Only 1 vial (P) for freezer A (C4)
Outbreak Data
UKG2007/36      

Shelf B17,

box B17 alpha,

rows C3 & D3

 

Added to collection on(25th Mar 2010) 

Blood cells washed in PBS [B329/07 2] Only 1 vial (P) for freezer A (C3)
and 1 vial (G) for freezer A (D3)


Outbreak Data
UKG2007/35   East Bergholt, Colchester  

Shelf B17,

box B17 α,

rows C2

 

(Added to collection on

25th Mar 2010)

  Blood cells washed in PBS [B329/07 1] Only 1 vial (P) for freezer A (C2)

Outbreak Data
UKG2007/34   Bures St. Mary, Bures  

Shelf B17,

box B17 α,

rows C1 and D1

 

Added to collection on

(25th March 2010)   

  Blood cells washed in PBS [B327/07 1] Only 1 vial (P) for freezer A (C1) and 1 vial (G) for freezer A (D1) 

Outbreak Data
UKG2007/33   Lawefarm, Swilland  

Shelf B17,

box B17 α,

row A10

Added to collection on

(25th March 2010)   

  Blood cells washed in PBS [B313/07 4] Only 1 vial (P) for freezer A (A10)

Outbreak Data

UKG2007/32

  BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

England:UK

 

Helmingham, Stowmarket

(CPH/38/096

/0002)

Cattle blood sample

 

Virus not yet  isolated

Shelf B17,

box B17 α,

rows A9 and B9

 

Added to collection on

(25th March 2010)  

(CPH/38/096/

0002)

 

from animal number

201854400364

Blood cells washed in PBS

(no passage in

cell culture)

[Stars No.

B309/07 55]

Only 1 vial (P) for freezer A (A9) and 1 vial (G) for freezer A (B9)

 

 

Outbreak Data

UKG2007/31

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 

England: UK

 

Helmingham, Stowmarket

(CPH/38/096

/0002)

Cattle blood sample

 

Virus not yet  isolated

 

Shelf B17,

box B17 α,

row A8

 

Added to collection on

(25th Mar 2010)

animal number

222422400219

Blood cells washed in PBS

[Stars No.

B309/07 52]

Only 1 vial (P) for freezer A (A8)

 

 Outbreak Data

UKG2007/30

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 

England: UK

Richerdson Farm,

E. Bergholt

(TM071350)

Cattle blood sample

 

Virus not yet  isolated

Shelf B17,

box B17 α,

rows A7 and B7

 

Added to collection on

(25th Mar 2010)

BTR2007/61

 

animal number

UK HAO 193 00404

Blood cells washed in PBS

[Stars No.

 B306/07 1]

Only 1 vial (P) for freezer A (A7) and 1 vial (G) for freezer A (B7) 

 

Outbreak Data

UKG2007/29

     

Shelf B17,

box B17 α,

row A6

 

Added to collection on

(25th Mar 2010)

 

Blood cells washed in PBS

[Stars No.

B302/07 128]

Only 1 vial (P) for freezer A (A6)

 

Outbreak Data

UKG2007/28

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 
   

Shelf B17,

box B17 α,

rows A5 and B5

 

Added to collection on

(25th March 2010)

  Blood cells washed in PBS [B302/07 128] Only 1 vial (P) for freezer A (A5) and 1 vial (G) for freezer A (B5) 

Outbreak Data
UKG2007/27

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR (2)

 
   

Shelf B17,

box B17 α,

row A4

 

Added to collection on

(25th March 2010    

  Blood cells washed in PBS [B302/07 118] Only 1 vial (P) for freezer A (A4)

Outbreak Data
UKG2007/26

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 

Coribs, Stowmarket

Shelf B17,

box B17 α,

rows A3 and B3

 

Added to collection on

(25th Mar 2010)

Blood cells washed in PBS [B302/07 115] Only 1 vial (P) for freezer A (A3) and 1 vial (G) for freezer A (B3) 

Outbreak Data
UKG2007/25

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR (2)

 
   

Shelf B17,

box B17 α,

rows A2 and B2

 

Added to collection on

(25th March 2010)

Blood cells washed in PBS

[B299/07 3]

Only 1 vial (P) for freezer A (A2) and 1 vial (G) for freezer A (B2) 

Outbreak Data

UKG2007/24

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 

Shelf B17,

box B17 α,

rows A1 and B1

 

Added to collection on

(25th March 2010) 

  Blood cells washed in PBS [B299/07 2] Only 1 vial (P) for freezer A (A1) and 1 vial (G) for freezer A (B1)



Outbreak Data
UKG2007/23

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 
Folly lane, Copedock  

Shelf B16,

box B16 g,

rows I10 and J10

 

Added to collection on

(25th March 2010)

  Blood cells washed in PBS [B299/07 1] Only 1 vial (P) for freezer A (I10) and 1 vial (G) for freezer A (J10) 

Outbreak Data
UKG2007/22

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 
   

Shelf B16,

box B16 g,

rows I9 and J9

 

Added to collection on

(25th March 2010) 

  Blood cells washed in PBS [B298/07 2] Only 1 vial (P) for freezer A (I9) and 1 vial (G) for freezer A (J9)

Outbreak Data
UKG2007/21

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR (2)

 
Gisleham, Lowestoft  

Shelf B16,

box B16 g,

rows I8 and J8

 

Added to collection on(25th Mar 2010)

  Blood cells washed in PBS [B298/07 1] Only 1 vial (P) for freezer A (I8) and 1 vial (G) for
freezer A (J8)

Outbreak Data

UKG2007/20

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 
Stock by Nayland, Colchester   Shelf B16, box B16γ, rows I7 and J7  

Added to collection on (25th Mar 2010)
  Blood cells washed in PBS [B295/07 1]

Only 1 vial (P) for freezer A (I7) and 1 vial (G) for
freezer A (J7)

Outbreak Data

UKG2007/19

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 
Tendring, Clacton on sea

Shelf B16,

box B16 g,

rows I6 and J6

 

Added to collection on

(25th March 2010)    

Blood cells washed in PBS [B293/07 1] Only 1 vial (P) for freezer A (I6) and 1 vial (G) for freezer A (J6)
UKG2007/18

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR  (2)

 
Sprougton, Ipswich  

Shelf B16,

box B16 g,

rows I5 and J5

 

Added to collection on

(25th Mar 2010)

  Blood cells washed in PBS [B286/07 24] Only 1 vial (P) for freezer A (I5) and 1 vial (G) for freezer A (J5) 
UKG2007/17

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR (2)

 
Beylham, Ipswich  

Shelf B16,

box B16 g,

rows I4 and J4

 

Added to collection on

(25th March 2010)  

  Blood cells washed in PBS [B265/07 186] Only 1 vial (P) for freezer A (I4) and 1 vial (G) for freezer A (J4) 

Outbreak Data
UKG2007/16

 BTV-8

 

Identified by serotype specific

 (Seg-2)

 RT-PCR (2)

 
Peatling Magna, Leicestershire  

Shelf B16,

box B16 g,

rows I3 and J3

 

Added to collection on

(25th Mar 2010)

  Blood cells washed in PBS [B260/07 1] Only 1 vial (P) for freezer A (I3) and 1 vial (G) for
freezer A (J3)

UKG2007/15

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

Essex, UK

(OS ref TL8222707858)

 

Sample taken

4th Oct 2007

Cattle blood, from

animal number UKS501800113

 

Isolated at

IAH-Pirbright

E6 E6 beta

Rows C1-D10

 

(Added to collection on  1st Nov 2007)

BTR2007/98

[IAH/E1/BHK2]

B356/07-1

Outbreak Data

Derived from blood sample UKG2007/78

UKG2007/14

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR (2)

Peterborough, UK

(OS ref TF153008)

 

Sample taken 14th Oct 2007

Sheep blood, from animal number UK223943000490

 

Isolated at

IAH-Pirbright

E6 E6 beta

Rows E1-F10

 

(Added to collection on  1st Nov 2007)

BTR2007/150

[IAH/E1/BHK2]

B411/07

Outbreak Data

generated from blood sample UKG2007/45
UKG2007/13

BTV-8

 

Identified by serotype specific (Seg-2)

 RT-PCR  (2)

Ashford, Kent, UK

(OS ref TR0090747618)

 

Sample taken 16th Oct 2007

Sheep blood from animal number UK2639222189

 

Isolated at

IAH-Pirbright

Shelf E6,

box E6β,

rows A1-B10

 

(Added to collection on  1st Nov 2007)
BTR2007/157 [IAH/E1/BHK2] [B418/07]

from same animal as UKG2007/12