Family: Reoviridae,  Genus: Orbivirus,  Species:   Bluetongue virus type 8 (BTV)

 * IAH dsRNA virus collection number

Serotype

(identification method)

(date original sample was taken)

Isolated from which host species

e.g cow,  sheep, etc

(Date of virus isolation, where, by whom)

(Date added to collection)

(Suppliers Isolate Designation)

(Passage history of sample)

[IAH virus diagnosis, ISIS sample number]

References

&

 accession numbers

[genome segments that have been sequenced]

RSArrrr/08

BTV Type 8

(17/12/82)

NK

Shelf B3,

Box B3γ,

Rows G1-H10

 (Added to collection on 20th May 2005)

E2/BHK6

[IAH/BHK1]

*

Duplicate samples G1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

* 

Duplicate samples,  box B3γ- position H1(p) supplied to Oscar R. González Llamazares (Avenida Párroco Pablo Díez 49-57, 24010 - Leon - Spain,  22nd Nov 2007)

* 

Vial H2 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial H3 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

* 

» contents

[S2:AJ585129,

S6:AJ586705]

Maan et al 2007

BTV-8

Identified as BTV 8 by Seg-2 specific

 Real time RT-PCR 

Israel,

 From Ashdot

Sample taken on

Shelf C9,

box α,

rows E1-F10

Added to collection

31st Aug 2010

Supplied by Jacob Brenner , Kimron Institute Israel

Samples arrived 4th August 2010

IAH Stars No.

BTV-8

Identified as BTV 8 by Seg-2 specific

 Real time RT-PCR 

Israel,

 From Hadera, ovine blood

 sampled

Shelf C7

box α,

rows A1-B10

E1

Jacob Brenner , Kimron Institute Israel

Samples arrived

4^th Aug 2010

IAH Stars

number

BTV-8

Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Ayelet Ha Shahar

sampled

Shelf A17

Box  A17 γ

Rows E1-F10

 ^{Added to
collection on
1st  Sept 2009}   

From: Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

16^th August  2009

IAH Stars number

BTV-8

Identified as BTV-8 by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Ayelet Ha Shahar

sampled

Shelf A17

Box  A17 γ

E1

From: Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived 16^th August 2009

IAH Stars number

OMN2009/04

BTV-8

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

Oman from Sohar, Batnah coast in the Arabian Gulf

Sample collected 13^th April 2009

ovine blood

Isolated in KC cells at IAH-P

Shelf B9

Box B9 alpha

Rows Added  G1- H10

Oman designation 16441

^[IAH/KC1]

Masha Japhet,

Oman

Samples arrived

15^TH May 2009

IAH Stars No. 

A42/09 37

BTV-8

Oman from Sohar, Batnah coast in the Arabian Gulf

Sheep blood

Isolated in

KC cells

at IAH-P

Shelf B9,

Box B9 α,

Masha Japhet,

Oman

Samples arrived

Approx 15^TH May 2009

IAH Stars No.

2008

DEN2008/02

BTV-8

Identified by LSI real time BTV 8 assay

From cattle

blood

Denmark  

Sample taken  from a calf

Originally isolated at IAH-Pirbright, from cattle blood

(sampling date 29th Aug 2008)

Shelf A12

Box A12 β,

rowsG1-H10

(Added to collection  26th Sept 2008)

[IAH/KC1]

DEN2008/01

BTV-8

Identified by LSI real time BTV 8 assay

From cattle

blood

Denmark  

Sample taken  from a cow

Originally isolated at IAH-Pirbright, from cattle blood

(sampling date 29th Aug 2008)

Shelf A12,

Box A12 β,

rows G1-H10

(Added to collection on  26th Sept 2008)

cow (009)

[IAH/KC1]

[Stars No.

A150/08 1]

GER2008/13

BTV-8

Identified by seg-2 specific real-time RT-PCR

From Germany

(sample taken

2008)

Positive blood from

field cattle

Shelf B18,

box B18 β ,

rows C1-D10

(Added to collection

29^th  July 2010)

from
Animal No. BH287/08_25

[IAH/KC1]

Derived from

GER2008/11

From Dr. Bernd Hoffman

Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman

From Dr. Bernd Hoffman

GRE2008/01

BTV-8

Identified by seg-2 specific RT-PCR

Greece:

Agia Paraskevi

Herd No:

EL83306344

from sheep

blood

Isolated at

IAH-Pirbright

1^st Dec 2008

Shelf A18,

box A18 β,

rows  I1 – J10

(added to collection

 10th Dec 2008)

Animal ID:

EL833063440340

[IAH/KC1]

Supplied by Olga Mangana

[Stars No. 

 A169/08 no 1]

BTV-8

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

Israel,

 from a village called: Rosh ha-nikra

samples

From bovine spleen,

Isolated in KC cells at IAH-P

Shelf B8, 

box B8γ,

Kimron institute

number

Dr. Hagai Yadin, Kimron Institute Israel.

Samples arrived

Approx

14^th  April 2009

IAH Stars No.

NET2008/03

BTV-8

 ~1yr old, unvaccinated animal

clear clinical signs of BT, including 

fever and conjunctivitis

 Shelf A14

Box A14 β,

Rows A1-B10

(Added to collection 

16^th Oct 2008)

(Animal ID 08016384)

[IAH/KC1/BHK1]

Derived from NET2008/01

Dr. Piet A. van Rijn, PhD

CVI Wageningen UR, Department of Virology P.O. Box 65, 8200 AB Lelystad, The Netherlands

NET2008/02

BTV-8

From Sheep blood (unvaccinated)

Netherlands: Bierum region

sample taken

15th Aug 2008.

clear clinical signs of BT

Shelf A14

Box A14 β,

Rows C1-D10

(Added to collection  16^th October 2008

(Animal ID 08017118-001)

[IAH/KC1]

Dr. Piet A. van Rijn, PhD

CVI Wageningen UR, Department of Virology P.O. Box 65, 8200 AB Lelystad, The Netherlands

Was seropositive

on first sampling

on 14th Aug 2008. 

Resampled

15th Aug 2008.

This was the 2^nd  case of BTV-8 in NET 2008.

NET2008/01

BTV-8

 ~1yr old, unvaccinated animal

clear clinical signs of BT, including 

fever and conjunctivitis

 Shelf A14

Box A14 β,

Rows E1-F10 

(Added to collection  16^th October 2008)

(Animal ID 08016384)

[IAH/KC1]

Used to generate NET2008/03

Dr. Piet A. van Rijn, PhD

CVI Wageningen UR, Department of Virology P.O. Box 65, 8200 AB Lelystad, The Netherlands

Was seropositive on first sampling 1st Aug 2008

Resampled on

7^th Aug 2008

SWE2008/02

Sweden 

From cattle

blood

Originally isolated at

IAH-Pirbright

7th Oct 2008

Shelf A14,

box A14 α ,

rows G1-H10

(added to collection

 9th Oct 2008)

Sample ID: 155300

[IAH/KC1]

samples supplied  by  Peter Thorén

Department of Virology, Immunobiology and Parasitology,

 Diagnostic Section

National  Veterinary Institute, SVA

[Stars No.

A157/08 1]

SWE2008/01

BTV-8

Identified by LSI real time BTV 8 assay

Sweden

From cattle

blood

Originally isolated at

IAH-Pirbright

7th Oct 2008

Shelf A14,

box A14 α ,

rows E1-F10

(added to collection

 9th Oct 2008)

Sample ID: 152951

[IAH/KC1]

UKG2008/41

BTV-8

Identified by group specific RT-PCR [1]

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3)

from calf blood

[IAH/

UKG2008/40

BTV-8 (blood)

Shelf B16,

only 1 vials (G) for freezer A

UKG2008/38

BTV-8 (blood)

UKG2008/37

BTV-8 (blood)

UKG2008/36

BTV-8 (blood)

UKG2008/35

BTV-8 (blood)

Shelf B16,

UKG2008/34

BTV-8

Identified by group specific RT-PCR [1]

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3)

Armoy, Ballymena,

Northern Ireland

Sample taken

13th Feb 2008

from calf blood

[IAH/

[Stars No.

B492/08 2]

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal

UK582544501188

Armoy, Ballymena,

Northern Ireland

(Sample taken

15th Feb 2008)

Originally isolated at 1AH direct bovine blood

(28th Feb 2008)

Shelf A16,

box A16 β,

rows I1-J10

(Added to collection 

 16^th Oct 2008)

[IAH/KC1/

BHK1]

Derived from

UKG2008/04

B520/08 1

UKG2008/32

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3)

Armoy, Ballymena,

Northern Ireland

Sample taken

13th Feb 2008

Originally isolated at 1AH-Pirbright

From cattle blood

(26th Feb 2008)

Animal

NL426303293

[IAH/KC1/

BHK1]

Derived from

UKG2008/06

The calf from this cow is represented by sample UKG2008/34

B492/08 1

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal  NL426303293

Armoy, Ballymena,

Northern Ireland

(14th Feb 2008)

Originally isolated at 1AH direct from spleen

(3rd March 2008)

Shelf A16,

box A16 β,

rows G1-H10

(Added to collection 

 16^th Oct 2008)

[IAH/KC1/

BHK1]

Derived from

UKG2008/03

B513/08 1

UKG2008/30

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal

UK9171373884-4

1 week old calf

Armoy, Ballymena,

Northern Ireland

(Sample taken

16th Feb 2008)

Originally isolated at 1AH direct bovine blood

(28th Feb 2008)

Derived from UKG2008/05

B522/08 1

Animal No. FR2306751274

UKG2008/28

BTV-8

Identified by LSI real time BTV 8 assay

From cattle

blood

England : UK

Sample taken  from a cow that was imported 4th Sept 2008 into Bromyard, England from central Germany

Originally isolated at IAH-Pirbright

From cattle blood

(sampling date 8TH Sept 2008)

Shelf A13

Box A13 GAMMA

I1-J10

Added 26/9/08

Animal No.  47804

[IAH/KC1]

[Stars No.

B1380/08 25]

single sample sample:

Misc  box, Freezer A,  Shelf E18, Box E18 α , position C12

UKG2008/27

BTV-8

Identified by LSI real time BTV 8 assay

From cattle

blood

England : UK

Sample taken  from a cow that was imported 4th Sept 2008

into Bromyard England from central Germany

Originally isolated at IAH-Pirbright

From cattle blood

(sampling date 8th Sept 2008)

Shelf A13,

box A13γ,

rows G1-H10

(Added to collection on 26th Sept 2008)

Animal No. 76720

[IAH/KC1]

[Stars No.

B1380/08 24]

Note only 9 vials of PDAM

single spare sample:

Misc  box, Freezer A, Shelf E18, Box E18 α ,  position C11

UKG2008/26

BTV-8

Identified by LSI real time BTV 8 assa

From cattle

blood

England : UK

Sample taken  from a cow that was imported 4th Sept 2008 into Bromyard, England from central Germany

Originally isolated at IAH-Pirbright

From cattle blood

(sampling date 8th Sept 2008)

Shelf A13,

Box A13 γ,

rows E1-F10

(Added to collection on 26th Sept 2008)

Animal No. 73991

[IAH/KC1]

[Stars No.

B1380/08 22]

Note only 9 vials of glycerol

single spare sample:

 Misc  box, Freezer A,  Shelf E18,ox E18 α , position C10

UKG2008/25

BTV-8

Identified by LSI real time BTV 8 assay

From cattle

blood

England: UK

Sample taken  from a cow that was imported 4th Sept 2008 into Bromyard, England from central Germany

Originally isolated at IAH-Pirbright 

from cattle  blood

(sampling date 8th Sept 2008)

Shelf A13,

Box A13 γ,

rows C1-D10

(Added to collection on 26th Sept 2008

Animal No.

21039

[IAH/KC1]

[Stars No.

B1380/08 20]

UKG2008/24

BTV-8

Identified by LSI real time BTV 8 assay

From cattle

blood

England

Sample taken  from a cow that was imported 4th Sept 2008 into Bromyard, England from central Germany

Originally isolated at IAH-Pirbright

From cattle  blood

(sampling date 8th Sept 2008)

Shelf A10,

Box A10β

rows I1-J10

(Added to collection on 26th Sept 2008

Animal No. 56314

[IAH/KC1]

[Stars No.

B1380/08 13]

UKG2008/23

UKG2008/22

UKG2008/21

UKG2008/20

UKG2008/19

Animal id DE0348696174

UKG2008/18

UKG2008/17

Animal id DE0348663886

UKG2008/16

Animal id DE0350330361

UKG2008/15

UKG2008/14

[Stars No.

B1340/08 1]

UKG2008/13

[Stars No.

B1346/08 35]

UKG2008/12

[Stars No.

B1346/08 30]

UKG2008/11

[Stars No.

B1346/08 21]

note only 8 PDAM in freezer A and B)

UKG2008/10

[Stars No.

B1346/08 20]

UKG2008/09

[Stars No.

B1346/08 17]

UKG2008/08

[Stars No.

B1346/08 16]

(note only 

9 vials PDAM

in freezer A and B)

UKG2008/07

BTV-8

Identified by serotype specific real time RT-PCR assay (LSI)

Sample taken  from a ram that was imported on 19th Aug 2008 to Lewes in Sussex,  England,  from central France

Originally isolated at 1AH-Pirbright from sheep blood

(sampling date 20th Aug 2008)

South Down Sheep

(no animal Id number)

[IAH/KC1]

[B1336/08 1 ]

UKG2008/06

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3)

Armoy, Ballymena,

Northern Ireland

Sample taken

13th Feb 2008

Originally isolated at 1AH-Pirbright

From cattle blood

(26th Feb 2008)

Shelf A8

Box A8 γ

Rows  G1-H10

 (added to collection on

4th April 2008)

Animal

NL426303293

[IAH/KC1]

Used to generate UKG2008/32

The calf from this cow is represented by sample UKG2008/34

B492/08 1

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal

UK9171373884-4

1 week old calf

Armoy, Ballymena,

Northern Ireland

(Sample taken

16th Feb 2008)

Originally isolated at 1AH direct bovine blood

(28th Feb 2008)

Shelf A9,

Box A9 γ

Rows I1-J10

 (added to collection on

4th April 2008)

[IAH/KC1]

Used to 

generate

UKG2008/30

B522/08 1

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal

UK582544501188

Armoy, Ballymena,

Northern Ireland

(Sample taken

15th Feb 2008)

Originally isolated at 1AH direct bovine blood

(28th Feb 2008)

Shelf A9

Box A9 γ

Rows G1-H10

 (added to collection on

4th April 2008)

Used to generate UKG2008/33

B520/08 1

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal  NL426303293

Armoy, Ballymena,

Northern Ireland

(14th Feb 2008)

Originally isolated at 1AH direct from spleen

(3rd March 2008)

Shelf A9

Box A9 γ

Rows E1-F10

 (added to collection on

4th April 2008)

[IAH/KC1]

Used to generate

UKG2008/31

B513/08 1

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal

582544501188

Armoy, Ballymena,

Northern Ireland

Sample taken 18/2/08

Originally isolated at 1AH direct from spleen

(3rd March 2008)

Shelf A9

Box A9 γ

Rows C1-D10

 (added to collection on

4th April 2008)

B534/08 3

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Animal

582544501188

Armoy, Ballymena,

Northern Ireland

Sample taken

18th Feb 2008

Originally isolated at 1AH direct from bovine blood

(3rd March 2008)

Shelf A9

Box A9 γ

Rows A1-B10

 (added to collection on

4th April 2008)

B534/08 3

SWI2007/01

BTV 8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

Basel, Switzerland

sample taken  7th Nov 2007

from cattle blood

into KC cells

Isolated at

IAH-Pirbright

Shelf A7

box A7 β

rows A1-B10

(Added to collection on  20th Dec 2007)

[IAH/KC2]

Barbara Thur,

Institut fur viruskrankheiten und immunprophylaxe, sensemattstr. 293, 3175 mittlehausern, switzerland

[A139/07 3]

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

Sakskøbing, STORSTRØMS

Denmark

Sample taken 11th Oct 2007

From sheep blood

Isolated at

IAH-Pirbright

Shelf E6,

bov E6β,

rows I1-J10

Original blood sample supplied by Anette Botner,

Lindholm

4771 Kalvehave Denmark

FRA2007/05

BTV-8

 (identified at Maisons Alfort, France)

North of Ardennes, France

(isolated at Maisons Alfort, France) 

Shelf E6,

Box E6α,

Rows G1-H10

 (Added to collection on  31st Oct 2007

3384

E1/BHK1

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

Outbreak data

BTV-8

 (identified at Maisons Alfort, France)

North of Ardennes, France

(isolated at Maisons Alfort, France) 

Shelf E5,

box E5γ,

rows  I1-J10

 (added to collection on 29th Oct 2007)

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

BTV-8

 (identified at Maisons Alfort, France)

North of Ardennes, France

(isolated at Maisons Alfort, France)

Shelf E5,

box E5γ,

rows G1-H10

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

BTV-8

 (identified at Maisons Alfort, France)

North of Ardennes, France

(isolated at Maisons Alfort, France) 

Shelf E5,

box E5γ,

rows E1-F10

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

BTV-8

 (identified at Maisons Alfort, France)

North of Ardennes, France

(isolated at Maisons Alfort, France)

Shelf E5,

box E5γ,

rows C1-D10

 (added to collection on 29th Oct 2007)

[IAH/BHK1]

Original virus isolate supplied  by Emmanuel Breard

UMR 1161 Afssa/INRA/ENVA,  22, Rue Pierre Curie,

Boite Postal 67, Maisons Alfort Cedex 94703, France.

Shelf B16,

Only 1 vial (P) for freezer A (I1) and 1 vial (G) for freezer A (J1) 

UKG2007/75

^{BTV-8 (blood)}

Shelf B16,

box B16 g,

rows I2 and J2

Added to collection on

(25th March 2010)    

Only 1 vial (P) for freezer A (I2) and 1 vial (G) for freezer A (J2) 

Shelf B16,

only 1 vials (G) for each freezer

BTV-8 (blood)

Shelf B16,

Shelf B16,

only 2 vials (G) for each freezer

BTV-8 (blood)

Shelf B16,

only 3 vials (G) for freezer A (E8-E10) and only 2 vials (G) for freezer B (E8-E9)

Shelf B16,

only 2 vials (G) for each freezer

BTV-8 (blood)

Shelf B16,

only 3 vials (G) for freezer A (F6-F8) and only 4 vials (G) for freezer B (F6-F9)

BTV-8 (blood)

Shelf B16,

only 9 vials (G) for freezer A (G1-G9) and only 10 vials (G) for freezer B (G1-G10)

Shelf B16,

only 1 vial (G) for freezer A

BTV-8 (blood)

Shelf B16,

only 1 vials (G) for freezer A

UKG2007/64

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3)

Original sample collected from a rare breeds farm, Baylham house , Baylham, Ipswich,  Suffolk

UK

(blood sample collected

21st Sept 2007)

Virus isolated from washed and sonicated EDTA treated cattle blood, applied directly to KC cells.

(isolated on ??)

??

UK index case BTR2007/24

from the highland cow called “Debbie”

[IAH/KC2]

Derived from UKG2007/06

Supplied to Intervet for vaccine trial in sheep

(10th Apr 2008)

Outbreak Data

BTV-8

Identified by serotype specific (Seg-2)

 RT-PCR (2)

 and sequence analyses (3) 

Pidgeon Hall Farm

Ipswich

EDTA Blood from ill neonatal calf. Died three days old

(Virus isolated at IAH –P on

20th Feb 2008)

 Shelf A9,

box A9 β,

rows A1-B10

 (added to collection on 13th March 2008)

Aho

Bury St Edmunds

Shelf B16,

Shelf B16,

Only 7 vials (P) for freezer A (D1-D7); 6 vials (P) for freezer B (D1-D6);  7 vials (G) each for freezer A and B (E1-E7)

^{Outbreak Data}

Shrubland, Coddenham

Shelf B16,

box B16 g,

rows C1-C10

Added to collection on

(25th March 2010)    

BTV-8

Maylane, Lowestoft Suffolk

Shelf B16,

box B16 g,

rows B1-B8

Added to collection on

Outbreak Data

Shelf B16,

Leavenheath, Suffolk

Shelf B17,

box B17 α,

rows G2 and H2

Added to collection on

Newton, Sudbury

Shelf B17,

box B17 α,

rows G1 and H1

Added to collection on

Nayland, Colchester

Shelf B17,

box B17 α,

rows E10 and F10

Added to collection on

Nayland, Colchester

Shelf B17,

Haddenham, Cambridgeshire

Shelf B16,

box B16 g,

rows H1-H5

Added to collection on

Woodlane, Oulton, Lowestoft

Shelf B17,

Outbreak Data

Stonestreet, Boxford

Shelf B17,

Outbreak Data

Tentender, Kent

Shelf B17,