ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Mixed or untyped or mixed isolates of BTV

Return to Virus Collection » Contents

 

*IAH dsRNA virus collection number: [Country code, year / sample number] rrrr = reference strain vvvv = vaccine strain]

 Family: Reoviridae,  Genus: Orbivirus,  Species: Bluetongue virus  untyped isolates

 

Virus Species

 

 

*IAH dsRNA virus collection number:

Serotype

 

(identification method)

Place of original sample

 

eg country town

[grid reference]

 

 

(date original sample was taken)

Isolated from which host species

e.g cow,  sheep, etc

 

(Date of virus isolation, where, by whom )

Position of storage in reference freezers

 

(Date added to collection)

 

 

(Suppliers Isolate Designation)

Adapted to which cell types

 

Original sample material

 

(Passage history of sample)

 Original sample supplied to the collection by

person / institution

 

[IAH virus diagnosis, ISIS sample number]

 Comments

 

References

&

 accession numbers

ISR2009/15

BTV-8  and BTV24

 

Identified as BTV 8 and 24 by Seg-2 specific

 Real time RT-PCR 

Israel

 

from Hadarom region Dekel,

 

 

Sheep sampled

1st Oct 2009

From sheep blood

 

Isolated through KC cells at IAH-P

Shelf B13

Box  B13β,

rows C1-D10,

 

 Added to collection  25th Nov 2009

3041/09

[IAH/KC1]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived 2nd November 2009

 

[IAH Stars No.

A88/09 01]

BTV-24 outbreak data

BTV-8 outbreak data
FRA2008/34 BTV-1
&
 BTV-8		  France

2008		 Isolated at AFSSA- Maisons Alfort France in 2008 Original tube labelled as BTV1/8.08.08 Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/33 BTV-1
&
 BTV-8		 France

2008		 Isolated at AFSSA- Maisons Alfort France in 2008   Original tube labelled as BTV1/8.08.07   Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/32 BTV-1
&
 BTV-8		 France

2008		 Isolated at AFSSA- Maisons Alfort France in 2008   Original tube labelled as BTV1/8.08.06   Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/31 BTV-1
&
 BTV-8		 France

2008		 Isolated at AFSSA- Maisons Alfort France in 2008   Original tube labelled as BTV1/8.08.05   Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/30 BTV-1
&
 BTV-8		 France

2008		 Isolated at AFSSA- Maisons Alfort France in 2008   Original tube labelled as BTV1/8.08.04   Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/29 BTV-1
&
 BTV-8		 France

2008		 Isolated at AFSSA- Maisons Alfort France in 2008   Original tube labelled as BTV1/8.08.03   Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/28 BTV-1
&
 BTV-8		 BTV-1
&
 BTV-8France

2008		 Isolated at AFSSA- Maisons Alfort France in 2008   Original tube labelled as BTV1/8.08.02   Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/27 BTV-1
&
 BTV-8		 France

2008		 Isolated at AFSSA- Maisons Alfort France in 2008   Original tube labelled as BTV1/8.08.01   Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
NET2008/11

BTV-6 and BTV-8

 

Identified by seg-2 specific RT-PCR

Netherlands:

Luttenburg

from cattle blood

 

Isolated at

IAH-P

18th Nov 2008

 

Shelf A17,

box A17 beta,

rows I1 –J10

Added to collection 20th Nov 2008

  

From cattle blood sample

[IAH/KC2/BHK1]

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 1]

ProMed Report

BTV-6 (vaccine strain)

Maan et al 2010
NET2008/09

BTV-6

 and

BTV-8

 

Identified by seg-2 specific RT-PCR

Netherlands:

Luttenberg

isolated at

 IAH P

7th Nov 2008

Shelf A17,

box A17 beta,

rows A1 –B10

added to collection

7th Nov 2008

 

From cattle blood sample

 

[IAH/KC2]

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 1]

ProMed Report

BTV-6 (vaccine strain)

 

See also BTV-6 blood samples NET2008/06 and NET2008/08

 

Maan et al 2010

GER2008/16

BTV-6 + 8

 

BTV-6 by

 LSI real time
Rrt-PCR

 but mixed for BTV-6 and 8 IAH assays

From Germany

 

(sample taken

2008)

 

Received at IAH

21st Jan 2009 

Positive blood from field cattle

Shelf B18,

box B18 β ,

rows G1-G6

 

(Added to collection

5th Aug 2010)

Animal No. BH 266/08 _1

Blood sample

From
Dr. Bernd Hoffman

Only 6 vials (P)
GER2008/15

BTV-6 + 8

 

BTV-6 by

 LSI real time
Rrt-PCR

 but mixed for BTV-6 and 8 IAH assays

From Germany

 

(sample taken

2008)

 

Received at IAH

21st Jan 2009

 Positive blood from field cattle

Shelf B18,

box B18 β ,

rows H1-H8

 

(Added to collection

5th Aug 2010)

 Animal No. BH 266/08 _2

Blood sample

From
Dr. Bernd Hoffman

   Only 8 vials (P)
GER2008/09

BTV-6 + 8

 

BTV-8 by

 LSI real time
Rrt-PCR

but mixed for BTV-6 and 8 by IAH assays 		From Germany     BH287/08_51 Blood sample From Dr. Bernd Hoffman Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman  
GER2008/08

BTV-6 + 8

 

BTV-8 by

 LSI real time
Rrt-PCR

but mixed for BTV-6 and 8 IAH assays		 From Germany     BH287/08_60 Blood sample From Dr. Bernd Hoffman Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman  

IND2005/05

BTV-10

also contains   BTV-1 and 23

India

  

Shelf D4

box D4 β,

rows C1-D10

 

(added to collection

22nd Nov2006)

Shelf D4,

box D4 β,

rows C1-D10

 

(added to collection

22nd Nov 2006) 

 [+IAH/ BHK3]

Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies

 

IND2005/04

BTV-10

also contains  BTV-23

India

  

Shelf D4

box D4 β,

rows E1-F10

 

(added to collection

22nd Nov2006)

Shelf D4,

box D4 β,

rows E1-F10

 

(added to collection

22nd Nov 2006) 

 [+IAH/ BHK3]

Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies

 

IND2004/03

Typed by

RT-PCR,

mixed BTV-1 and BTV-9 plus possible

(doubtful) signal for BTV-21

India,

Andhra Pradesh

 

Sample taken

 2004

From Blood

Shelf D4,

box D4 α,

rows C1-D10

 

(added to collection

17th Nov 2006)

No. 3

 [+IAH/BHK3]

   Sample given to Sushila maan IAH Pirbright Sept 2006 for sequencing studies  

IND2004/01

Typed by

RT-PCR ,

mixed BTV-9 and BTV-10 plus possible (doubtful)signal for BTV-21

India,

Andhra Pradesh

 

Sample taken

 2004

From Blood

Shelf D4,

box D4 α,

rows A1-B10

 

(added to collection

17th Nov 2006)

No. 1

 [IAH/ BHK3]

   Sample given to Sushila maan IAH Pirbright Sept 2006 for sequencing studies  

ISR2001/19

 

BTV

 

 

2, 4, 6, 10, 16

Israel,

Yokneam

Sheep (01/Mar/2001 at the Kimron Institute)

N/A

(300180

Lab No:824)

CE

 Dr. Hagai Yadin,

 Kimron Institute Israel

(MTA)

 

Failed to grow

 in cell culture

» contents

TUR2000/06

 

 

 

 

BTV

Type 9  & 16

 

( Species by ELISA

 

Typed by

RT-PCR)

Turkey

from IZMIR-Torbali

 

 

 

(year 2000)

 

 

(BTTR 2000/6)

(E2/BHK5)

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey

 

06/10/00

[R13/00 no 6]

 

» contents

 

 

 

[S10:   ]

TUR2000/07

 

 

 

 

BTV

Type 9  & 16

 

( Species by ELISA

 

Typed by

RT-PCR)

Turkey

from Aydin

 

(year 2000)

 

shelf C2,

box C2β,

rows E1-F10

 

Added to collection on

25th Oct 2005 

 

 

 

(BTTR 2000/7)

(E2/BHK5)

 

[IAH/BHK3]

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey

 

06/10/00

[R13/00 no 7]

 

 

[S10:   ]

ZIM2003/07

 

BTV

?????

Zimbabwe

(Lone Oak)

2003

Culicoides imicola

(25/08/03)

 

(E1/BHK1)

  [R06/03,No.150]

  Genone segment 2 sequence in progress

 

CYP2004/02

BTV Type 4+16

 

(Identified as BTV by ELISA & Typed by serum neutralisation)

Ammochostos,

Cyprus

 

 (12-2-2004)

Sheep (spleen)

 

 Isolated on 10-3-2004 at: Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou &  O.Mangana

3oth Sept. 2004

CY1025/1/04

 

Animal code: 1025/1

CPAE& BHK 21 Cells Spleen CPAE1 BHK 4

Nomikou Kiki

 

Centre of Athens

Vet. Institutions,

Virus Lab, Athens.

 

 
                 

NIG1982/05

BTV Type 5/6

Nigeria (23/11/82)

NK  

 

(N-456)

E2/BHK3

 

 

 

 

 

References

1. Shaw AE, Monaghan P, Alpar HO, Anthony S, Darpel KE, Batten CA, Guercio A, Alimena G, Vitale M, Bankowska K, Carpenter S, Jones H, Oura CA, King DP, Elliott H, Mellor PS, Mertens PP. (2007) Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1. J Virol Methods. 45, 115-126.

2. Sushila Maan, Narender S. Maan, Piet A.van Rijn, René G.P. van Gennip, Anna Sanders, Isabel M. Wright, Carrie Batten, Bernd Hoffmann, Michael Eschbaumer, Chris A.L. Oura, Abraham. C. Potgieter, Kyriaki Nomikou, and Peter P.C. Mertens (2010) Full genome characterisation of bluetongue virus serotype 6 from the Netherlands 2008 and comparison to other field and vaccine strains (in Press)

 

 

 

 

 

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