ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Mixed or untyped or mixed isolates of BTV

Return to Virus Collection » Contents

 

2010 2009 2008 2005 2004 2003 2001 2000 1997 1996 1989 1982 1981

 

*IAH dsRNA virus collection number: [Country code, year / sample number] rrrr = reference strain vvvv = vaccine strain]

 Family: ReoviridaeGenus: Orbivirus,  Species: Bluetongue virus  untyped isolates

 

Virus Species

 

 

*IAH dsRNA virus collection number:

Serotype

 

(identification method)

Place of original sample

 

eg country town

[grid reference]

 

 

(date original sample was taken)

Isolated from which host species

e.g cow,  sheep, etc

 

(Date of virus isolation, where, by whom )

Position of storage in reference freezers

 

(Date added to collection)

 

 

(Suppliers Isolate Designation)

Adapted to which cell types

 

Original sample material

 

(Passage history of sample)

 Original sample supplied to the collection by

person / institution

 

[IAH virus diagnosis, ISIS sample number]

Comments

 

References

&

 accession numbers

2010
ISR2010/16

BTV-4 and 8 Identified as BTV-4 and 8 by Seg-2 specific

 Real time RT-PCR 

07/11/10 Tzipori, Israel Sheep blood

Shelf C12

Box  C12 beta Rows C and D

Added  21/1/11 (Note only 9 of pdam and glycerol in each freezer)
2944-2 KC1

Jacob Brenner

Kimron Institute Israel

 

 

Samples arrived 24th November 2010

 

IAH Stars
number
A114/10 15
 
ISR2010/15

BTV-4 and 8 Identified as BTV-4 and 8 by Seg-2 specific

 Real time RT-PCR 

24/10/10 Tel Adashim, Israel Cattle blood

Shelf C12

Box  C12 beta Rows A and B

Added  21/1/11 (Note only 9 of pdam and glycerol in each freezer)
2828 KC1

Jacob Brenner

Kimron Institute Israel

 

 

Samples arrived 24th November 2010

 

IAH Stars
number
A114/10 13
 
2009
AUS2009/02

BTV
Untyped

Australia
NSW

Cow
(2009)

-     -

Old isolate No.: D439

new isolate
No.: E047

 Origin: EMAI 05/459

 

Sample sent as 'BTV1-21'

1ECE.1AA, 2BHK21, 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

AUS2009/01

BTV
Untyped

Australia
NSW

Cow
(2009)

 

Old isolate No.: D432

new isolate
No.: E046

 Origin: EMAI 09/776

 

Sample sent as 'BTV 1-21'

1ECE.1AA, 2BHK21, 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

ISR2009/15

BTV-8  and BTV24

 

Identified as BTV 8 and 24 by Seg-2 specific

 Real time RT-PCR 

Israel

 

from Hadarom region Dekel,

 

 

Sheep sampled

1st Oct 2009

From sheep blood

 

Isolated through KC cells at IAH-P

Shelf B13

Box  B13β,

rows C1-D10,

 

 Added to collection  25th Nov 2009

3041/09

[IAH/KC1]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived 2nd November 2009

 

[IAH Stars No.

A88/09 01]

BTV-24 outbreak data

BTV-8 outbreak data

2008
FRA2008/34 BTV-1
&
 BTV-8
 France

2008
Isolated at AFSSA- Maisons Alfort France in 2008

Shelf C10,

box C10 α ,

rows E1-F10

 

Added to collection

30th September

2010

Original tube labelled as BTV1/8.08.08

[IAH/BHK1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/33 BTV-1
&
 BTV-8
France

2008

Isolated at AFSSA- Maisons Alfort France in 2008

Shelf C10,

box C10 β ,

rows E1-F10

 

Added to collection

8th October 2010

Original tube labelled as BTV1/8.08.07

[IAH/BHK1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/32 BTV-1
&
 BTV-8
France

2008
Isolated at AFSSA- Maisons Alfort France in 2008

Shelf C10,

box C10 β ,

rows G1-H10

 

Added to collection

8th October 2010

Original tube labelled as BTV1/8.08.06

[IAH/Vero1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/31 BTV-1
&
 BTV-8
France

2008
Isolated at AFSSA- Maisons Alfort France in 2008

Shelf C10,

box C10 α ,

rows C1-D10

 

Added to collection

30th Sept 2010

Original tube labelled as BTV1/8.08.05

[IAH/BHK1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/30 BTV-1
&
 BTV-8
France

2008
Isolated at AFSSA- Maisons Alfort France in 2008

Shelf C10,

box C10 α ,

rows I1-J10

 

Added to collection

30th Sept2010

Original tube labelled as BTV1/8.08.04

[IAH/BHK1 ]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/29 BTV-1
&
 BTV-8
France

2008
Isolated at AFSSA- Maisons Alfort France in 2008

Shelf C10,

box C10 α ,

rows A1-B10

 

Added to collection

30th Sept 2010

Original tube labelled as BTV1/8.08.03

[IAH/BHK1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/28 BTV-1
&
 BTV-8
BTV-1
&
 BTV-8France

2008
Isolated at AFSSA- Maisons Alfort France in 2008

Shelf C10,

box C10 α ,

rows G1-H10

 

Added to collection

30th Sept 2010

Original tube labelled as BTV1/8.08.02

[IAH/BHK1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
FRA2008/27 BTV-1
&
 BTV-8
France

2008
Isolated at AFSSA- Maisons Alfort France in 2008

Shelf C9,

box C9 γ ,

rows I1-J10

 

Added to collection

15th Sept 2010

Original tube labelled as BTV1/8.08.01 [IAH/BHK1] Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material from an unvaccinated animal
NET2008/11

BTV-6 and BTV-8

 

Identified by seg-2 specific RT-PCR

Netherlands:

Luttenburg

from cattle blood

 

Isolated at

IAH-P

18th Nov 2008

 

Shelf A17,

box A17 beta,

rows I1 –J10

Added to collection 20th Nov 2008

 

From cattle blood sample

[IAH/KC2/BHK1]

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 1]

ProMed Report

BTV-6 (vaccine strain)

Maan et al 2010

NET2008/09

BTV-6

 and

BTV-8

 

Identified by seg-2 specific RT-PCR

Netherlands:

Luttenberg

isolated at

 IAH P

7th Nov 2008

Shelf A17,

box A17 beta,

rows A1 –B10

added to collection

7th Nov 2008

 

From cattle blood sample

 

[IAH/KC2]

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A163/08 no 1]

ProMed Report

BTV-6 (vaccine strain)

 

See also BTV-6 blood samples NET2008/06 and NET2008/08

 

Maan et al 2010

GER2008/16

BTV-6 + 8

 

BTV-6 by

 LSI real time
Rrt-PCR

but mixed for BTV-6 and 8 IAH assays

From Germany

 

(sample taken

2008)

 

Received at IAH

21st Jan 2009 

Positive blood from field cattle

Shelf B18,

box B18 β ,

rows G1-G6

 

(Added to collection

5th Aug 2010)

Animal No. BH 266/08 _1

Blood sample

From
Dr. Bernd Hoffman

Only 6 vials (P)

GER2008/15

BTV-6 + 8

 

BTV-6 by

 LSI real time
Rrt-PCR

but mixed for BTV-6 and 8 IAH assays

From Germany

 

(sample taken

2008)

 

Received at IAH

21st Jan 2009

Positive blood from field cattle

Shelf B18,

box B18 β ,

rows H1-H8

 

(Added to collection

5th Aug 2010)

Animal No. BH 266/08 _2

Blood sample

From
Dr. Bernd Hoffman

  Only 8 vials (P)
GER2008/09

BTV-6 + 8

 

BTV-8 by

 LSI real time
Rrt-PCR

but mixed for BTV-6 and 8 in  IAH assays

From Germany

(sample taken

2008)

 

Received at IAH   Received at IAH

21st Jan 2009 

Positive blood from field cattle

Shelf B17,

box B17 γ ,

rows F5-F7

 

Added to collection

9th  April 2010

BH287/08_51 Blood sample From Dr. Bernd Hoffman

Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman


Note: Only 3 vials (P)
 
GER2008/08

BTV-6 + 8

 

BTV-8 by

 LSI real time
Rrt-PCR

but mixed for BTV-6 and 8 in IAH assays

From Germany

(sample taken

2008)

 

 Received at IAH

21st Jan 2009 

Positive blood from field cattle

Shelf B17,

box B17 γ ,

rows F1-F3

 

Added to collection

9th  April 2010

BH287/08_60 Blood sample From Dr. Bernd Hoffman

Note MTA: Not provided to other parties without written permission from Bernd Hoffman : also  data published  from this strain, must acknowledge and inform Bernd Hoffman


Note: Only 3 vials (P)
2005

AUS2005/02

BTV
Untyped

Australia
NSW

Cow
(2005)

 

Old isolate No.: B478

new isolate
No.: E043

 Origin: EMAI 05/983

 

Sample sent as 'BTV1-21'

1ECE.1AA, 2BHK21, 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

AUS2005/01

BTV
Untyped

Australia
NSW

Cow
(2005)

 

Old isolate No.: A443

new isolate
No.: E042

 Origin: EMAI 03/1123

 

Sample sent as 'BTV1-21'

1ECE.1AA,3BHK21, 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

IND2005/06 BTV-2
&
BTV-9
Southern India From sheep Shelf ???,
box ??? ,
rows ???

(Added to collection 2006)
Unknown passage level in BHK cells

 [IAH/BHK1]
  Sample given to Sushila Maan (IAH Pirbright) Sept 2006 for sequencing studies
*
(MTA)

IND2005/05

BTV-10

also contains   BTV-1 and 23

India

 

Shelf D4

box D4 β,

rows C1-D10

 

(added to collection

22nd Nov2006)

Shelf D4,

box D4 β,

rows C1-D10

 

(added to collection

22nd Nov 2006) 

 [+IAH/ BHK3]

Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies

(MTA)

IND2005/04

BTV-10

also contains  BTV-23

India

 

Shelf D4

box D4 β,

rows E1-F10

 

(added to collection

22nd Nov2006)

Shelf D4,

box D4 β,

rows E1-F10

 

(added to collection

22nd Nov 2006) 

 [+IAH/ BHK3]

Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies

(MTA)
2004

CYP2004/02

BTV Type 4+16

 

(Identified as BTV by ELISA & Typed by serum neutralisation)

Ammochostos,

Cyprus

 

 (12-2-2004)

Sheep (spleen)

 

 Isolated on 10-3-2004 at: Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou &  O.Mangana

 

CY1025/1/04

 

Animal code: 1025/1

 3oth Sept. 2004

CPAE& BHK 21 Cells Spleen CPAE1 BHK 4

Nomikou Kiki

 

Centre of Athens

Vet. Institutions,

Virus Lab, Athens.

 

 

IND2004/03

Typed by

RT-PCR,

mixed BTV-1 and BTV-9 plus possible

(doubtful) signal for BTV-21

India,

Andhra Pradesh

 

Sample taken

 2004

From Blood

Shelf D4,

box D4 α,

rows C1-D10

 

(added to collection

17th Nov 2006)

No. 3

 [+IAH/BHK3]

  Sample given to Sushila maan IAH Pirbright Sept 2006 for sequencing studies (MTA)

IND2004/01

Typed by

RT-PCR ,

mixed BTV-9 and BTV-10 plus possible (doubtful)signal for BTV-21

India,

Andhra Pradesh

 

Sample taken

 2004

From Blood

Shelf D4,

box D4 α,

rows A1-B10

 

(added to collection

17th Nov 2006)

No. 1

 [IAH/ BHK3]

  Sample given to Sushila maan IAH Pirbright Sept 2006 for sequencing studies (MTA)
2003
AUS2003/02 BTV
Untyped
Australia
NSW

(2003)
Cow

Old isolate No.: A903

new isolate
No.: E74

 Origin: EMAI 03/0740

 

Sample sent as 'BTV1-21'

  1ECE.1AA,1BHK21, 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

AUS2003/01

BTV
Untyped

Australia
NSW

 

(2003)

Cow

Old isolate No.: A913

new isolate
No.: E041

 Origin: EMAI 03/1123

 

Sample sent as 'BTV1-21'

_         _

1ECE.1AA,1BHK21, 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI.

 

(MTA)

ZIM2003/07

 

 

BTV

?????

Zimbabwe

(Lone Oak)

2003

Culicoides imicola

 

 (25/08/03)

(E1/BHK1)

  [R06/03,No.150]

  Genone segment 2 sequence in progress

 

2001

ISR2001/19

 

BTV

 

 

2, 4, 6, 10, 16

Israel,

Yokneam

Sheep (01/Mar/2001 at the Kimron Institute)

N/A

(300180

Lab No:824)

CE

 Dr. Hagai Yadin,

 Kimron Institute Israel

(MTA)

 

Failed to grow

 in cell culture

» contents

2000

TUR2000/06

 

 

 

 

 

BTV

Type 9  & 16

 

( Species by ELISA

 

Typed by

RT-PCR)

Turkey

from IZMIR-Torbali

 

 

 

(year 2000)

 

 

(BTTR 2000/6)

(E2/BHK5)

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey

 

06/10/00

[R13/00 no 6]

 

» contents

 

 

 

[S10:   ]

TUR2000/07

 

 

 

 

 

BTV

Type 9  & 16

 

( Species by ELISA

 

Typed by

RT-PCR)

Turkey

from Aydin

 

(year 2000)

 

shelf C2,

box C2β,

rows E1-F10

 

Added to collection on

25th Oct 2005 

 

 

 

(BTTR 2000/7)

(E2/BHK5)

 

[IAH/BHK3]

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey

 

06/10/00

[R13/00 no 7]

 

 

[S10:   ]

1997
AUS1997/02

BTV
Untyped

Australia
NSW

 

(1997)

Cow

Old isolate No.: Z177

new isolate
No.: E054

 Origin: EMAI 97/0126

 

Sample sent as 'BTV1-21'

  1ECE/1AA/1BHK21/ 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

AUS1997/01

BTV
Untyped

Australia
NSW

 

(1997)

Cow

Old isolate No.: Z169

new isolate
No.: E053

 Origin: EMAI 97/0777

 

Sample sent as 'BTV1-21'

1ECE/1AA/1BHK21/ 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

1996
AUS1996/03

BTV
Untyped

Australia
NSW

 

(1996)

Cow

Old isolate No.: Z66

new isolate
No.: E052

 Origin: EMAI 96/4010

 

Sample sent as 'BTV1-21'

  1ECE/1AA/1BHK21/ 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

1989
AUS1989/02

BTV
Untyped

Australia
NSW

 

(1989)

Cow

Old isolate No.: X347

new isolate
No.: E051

 Origin: EMAI 89/272

 

Sample sent as 'BTV1-21'

  1ECE/1AA/1BHK21/ 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

AUS1989/01

BTV
Untyped

Australia
NSW

 

(1989)

Cow

Old isolate No.: X329

new isolate
No.:  E050

 Origin: EMAI 89/70

 

Sample sent as 'BTV1-21'

1ECE/1AA/1BHK21/ 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL

 

(MTA)

1982

NIG1982/05

BTV Type 5/6

Nigeria (23/11/82)

NK  

 

(N-456)

E2/BHK3

 

 

 

 

 

1981
AUS1981/12

BTV
Untyped

Australia
NSW

 

(1981)

Cow

Old isolate No.: W511

new isolate
No.: E90

 Origin: EMAI 81/502

 

Sample sent as 'BTV1-21'

  1ECE/5BHK21/ 1HmLu-1

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI.

 

(MTA)

References

1. Shaw AE, Monaghan P, Alpar HO, Anthony S, Darpel KE, Batten CA, Guercio A, Alimena G, Vitale M, Bankowska K, Carpenter S, Jones H, Oura CA, King DP, Elliott H, Mellor PS, Mertens PP. (2007) Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1. J Virol Methods. 45, 115-126.

2. Sushila Maan, Narender S. Maan, Piet A.van Rijn, René G.P. van Gennip, Anna Sanders, Isabel M. Wright, Carrie Batten, Bernd Hoffmann, Michael Eschbaumer, Chris A.L. Oura, Abraham. C. Potgieter, Kyriaki Nomikou, and Peter P.C. Mertens (2010) Full genome characterisation of bluetongue virus serotype 6 from the Netherlands 2008 and comparison to other field and vaccine strains (in Press)

 

 

 

 

 

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