*IAH dsRNA virus collection number

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 (identification method)

Place of original sample eg country town

 [grid reference]

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 Adapted to which cell types 

(Passage history of sample)

[IAH passage history]

* Original sample supplied to the collection by person / institution

[IAH, ISIS sample number]

Comments

 References &  accession numbers  

BTV

Type 3

NK

Shelf B2, boxB2α,

rowsC1-D10 

(16/Mar/2005)

 (BTV-3 Reference  Strain)  

E1/BHK4

[IAH-BHK2]

Supplied by OVI

S. Africa* (07/10/83) 

Duplicate samples C1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong,JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea.

Vial D1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial D2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

* 

» contents

 [S2:AJ585124,  S6:AJ586697 S10: ]

Maan et al 2007

BTV- 24

Virus serotype identified by RT-PCR using type specific primers  and sequencing (Seg-2)   [1].

USA: Marion County Florida

Sample taken 25th  Sept  2007

White tailed deer  (farmed)

Multiple losses and Clinical disease observed in herd

Isolated in 2007

Virus ID: 07-517030

Tube labelled:

517030; IVD BHK1; 10-30-07

(sample prepared

30th Oct 2007)

Isolated from spleen sample

E1/BHK1

virus isolate supplied by / Reagent also available from:

 : 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

nvsluf@aphis.usda.gov

Technical contact in USA:

Donna Johnson

:

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

BTV- 3

Initial attempts to type the virus at SCWDS were unsuccessful.

Virus serotype identified by RT-PCR using type specific primers  and sequencing (Seg-2)   [1].  Confirmed by SNT at NVSL.

USA: Yalobusha County

Sample taken in the fall of  2006

White tailed deer 

Multiple losses and Clinical disease observed in herd

Isolated at the Southeastern  Cooperative Wildlife Disease Study (SCWDS) Athens  GA.

Shelf A15,

box A15 γ,

rows I1-J10

(Added to collection 

 16^th Oct 2008)

Virus ID:06-473906-7

Tube labelled:

473906-7; orig isolate to BHK/2; 1-3-07

(sample prepared

3rd Jan 2007)

Isolated from tissue sample

CPAE 1/BHK2

virus isolate supplied by / Reagent also available from:

 : 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

nvsluf@aphis.usda.gov

Technical contact in USA:

Donna Johnson

:

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

(82% similar (Seg-2) to other BTV-3 isolates from Florida

(1130 bp region))

BTV-3

(Seg-2 RT-PCR and sequencing, suggests BTV-3, however only 1 set of BTV-3 specific primers out of 5 gave +ve amplification.  82% sequence homology was detected in 675bp of Seg-2 to FL isolates of BTV-3 (which show 91-98% homology to each other).  This places the virus as BTV-3 (Maan et al 2007)

A virus neutralisation assay also indicated

 BTV-3

although the type specificity of the BTV-3 sera is unconfirmed.

Further confirmation is requested

Yalobusha County, MS,

USA

(sample taken

during the fall of 2006)

From White tailed deer

(no clinical history)

Date of virus

 preparation in USA

3rd Jan 2007

Shelf E2,

box E2 γ,

rows A1-B10 

Added to collection

13th Sep 2007

06-473906-7

(tube labelled: 473906-7;

orig isolate to BHK/2;

1-3-078)

From blood

*CPAE1/BHK2

[IAH/BHK4 ]

* Note: the CPAE cell cultures used for isolation are infected with bovine viral diarrhoea (BVD) virus

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

Original isolate was submitted from the Southeastern Cooperative Wildlife Disease Study (SCWDS) Athens GA. isolate obtained from tissues samples collected in the fall of 2006 in Yalobusha County MS. At SCWDS, samples were innoculated into cattle pulmonary artery endothelial (CPAE) cells cultures. sample sent to NVSL for typing.

from an unvaccinated cow on

“la Réunion”

2003

Supplied by Emmanuel Breard,

AFSSA-Maisons Alfort-France

21st July 2010

REU2003/01

BTV-3

from

“la Réunion”

2003

isolated at AFSSA- Maisons Alfort France

in 2003

Shelf E3,

box E3 α,

Added to collection

13th Sep 2007

1567 W1B2: BTV3

E1/BHK2

[IAH/BHK2]

Supplied by Stephan Zientara and Emmanuel Breard,

AFSSA-Maisons Alfort-France

BTV-3

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-3 antisera).

Manatee County, FL,

USA

(sample taken

21th Oct 2003)

From cattle

(no clinical history)

Date of virus

 preparation in USA

12th Nov 2003

Shelf E3,

box E3 α,

rows A1-B10 

Added to collection

13th Sep 2007

04-280559-9

(tube labelled: 280559-9;

IVD YSD BHK/1;

11-12-03)

From blood

E1/BHK1

[IAH/ BHK3]

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

Multiple serotypes of BTV were isolated from this herd, including BTV2, 3, 10 (2 isolates), 5,

10 and 19. See also USA2003/06

BTV-3

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-3 antisera)

Manatee County, FL,

USA

(sample taken

21th Oct 2003)

From cattle

(no clinical history)

Date of virus

 preparation in USA

2nd Dec 2003

Shelf E2,

box E2 γ,

rows I1-J10 

Added to collection

13th Sep 2007

04-280559-1

(tube labelled: 280559-1;

IVD YSD BHK/1;

12-2-03 BT)

From blood

E1/BHK1

[IAH/BHK3 ]

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

Multiple serotypes of BTV were isolated from this herd, including BTV2, 3, 10 (2 isolates), 5,

10 and 19.   See also USA2003/07

 ZIM2002/01

BTV

Type 3  

Zimbabwe (10/08/02)

Cattle

from animal number 0505

Rows G1-H10

(R11/02 (24))

E1/BHK1

[IAH-BHK1] 

aliquots 1/10a, 1/10b pDam, Sent to Kiki Nomikou, Athens Vet Labs ,  Greece, Oct 2004

 [S2: S6:AJ586698]

 ZIM2002/02

BTV

 Type 3 

Zimbabwe  (22/08/02)

Cattle

from animal number 0510

 shelf C5

 box C5α

rows C1-D10

(Added to collection on

22nd Dec 2005)

(R11/02 (31))

E1/BHK1

[IAH/BHK2]

BTV-3

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-3 antisera)

Ocheechobee County, FL,

USA

(sample taken

30th Oct 2002)

From cattle

(no clinical history)

Date of virus

 preparation in USA

7h Aug 2006

Shelf E2,

box E2 γ,

Added to collection

03-220082-16

(tube labelled: 220082-16;

IVD BHK/1;

8-7-06)

From blood

E1/BHK1/E1BHK1

[IAH/BHK3 ]

(note: repeated passage of the original virus gave low titres, so virus was re-inoculated into eggs (July 2006) followed by one pass in BHK cells)

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

Multiple serotypes of BTV were isolated from this herd, including BTV3, 10 (3 isolates), and 22.  

 Sample originally submitted for export testing

BTV-3

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-3 antisera)

Alachua County, FL,

USA

(sample taken

23rdAug 2002)

From Deer

(no clinical history)

Date of virus

 preparation in USA

8th Aug 2006

 Shelf E2,

box E2 β,

Added to collection

02-202795

(202795;

IVD BHK/1;

 8-8-06)

From blood

E1/BHK1/E1BHK1

[IAH/ BHK3]

(note: repeated passage of the original virus gave low titres, so virus was re-inoculated into eggs (July 2006) followed by one pass in BHK cells)

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

BTV-3

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-3 antisera)

Martin County, FL,

USA

(sample taken

5-6th Nov 2001)

From Deer

(no clinical history)

Date of virus

 preparation in USA 25th Aug 2006

 Shelf E2,

box E2 γ,

Added to collection

02-138555-43

(138555-43; IVD BHK/1;

+BT;

8-25-06)

From spleen

E1/BHK3/E1BHK1

[IAH/ BHK2]

(note: repeated passage of the original virus gave low titres, so virus was re-inoculated into eggs (July 2006) followed by one pass in BHK cells)

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

Samples from 3 deer were originally submitted to USDA.  BTV-3 was isolated from all 3 animals

USA2001/14

BTV-3

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-3 antisera)

Martin County, FL,

USA

(sample taken

5-6th Nov 2001)

From Deer

(no clinical history)

Date of virus

 preparation in USA 9th Aug 2006

Shelf E3,

box E3 α,

Added to collection

02-138555-30

(138555-30; IVD BHK/1;

8-9-06)

From Blood

E1/BHK3/E1BHK1

[IAH/BHK3 ]

(note: repeated passage of the original virus gave low titres, so virus was re-inoculated into eggs (July 2006) followed by one pass in BHK cells)

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

Samples from 3 deer were originally submitted to USDA.  BTV-3 was isolated from all 3 animals

USA1999/07

BTV-3

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-3 antisera)

Highlands County, FL,

USA

(sample taken

Dec 1999)

From sheep

(clinical signs of BT)

Date of virus

 preparation in USA 7th Aug 2006

Shelf E2,

box E2 γ,

rows G1-H10

Added to collection

13th Sep 2007

00-22364-10

(22364-10; IVD BHK1; 8-7-06)

From Blood

E1/BHK1/E1BHK1

[IAH/ BHK3]

(note: repeated passage of the original virus gave low titres, so virus was re-inoculated into eggs (July 2006) followed by one pass in BHK cells)

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

See also USA1999/06

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

Note: BTV-2 was identified in an animal from this flock in November 1999.  This was the first isolation of  BTv-2 in the USA for over 16 years.  the flock was comprised of ~1000 sheep that had moved from New Hampshire to Florida in the fall of 1999. numerous animals were exhibiting clinical symptoms of BT. Multiple serotypes of BT virus were isolted from this flock (BTV-2,  3, 13 amd 17) 

USA1999/06

BTV-3

(typed by

Seg-2 RT-PCR and sequencing, confirmed by  neutralisation by BTV-3 antisera)

Highlands County, FL,

USA

(sample taken

Dec 1999)

From sheep

(clinical signs of BT)

Date of virus

 preparation in USA 7th Aug 2006

 Shelf E2,

box E2 β,

Added to collection

13th Sep 2007

00-22364-4

(22364-4; IVD BHK1; 8-7-06)

From Blood

E1/BHK1/E1BHK1

[IAH/ BHK3]

(note: repeated passage of the original virus gave low titres, so virus was re-inoculated into eggs (July 2006) followed by one pass in BHK cells)

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

See also USA1999/07

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

Note: BTV-2 was identified in an animal from this flock in November 1999.  This was the first isolation of  BTv-2 in the USA for over 16 years.  the flock was comprised of ~1000 sheep that had moved from New Hampshire to Florida in the fall of 1999. numerous animals were exhibiting clinical symptoms of BT. Multiple serotypes of BT virus were isolted from this flock (BTV-2,  3, 13 amd 17) 

BTV-3

Initial attempts to identify serotype by SNT were unsuccessful  in 1990

Identified as type 3 by  specific RT-PCR  and sequencing (Seg-2) 

 March 2008  [1]

Trinidad:

St. Andrews

Sample taken 4th Sept 1990

Water buffalo blood

Isolated in 1990

Shelf A15,

box A15 γ,

rows E1-F10

(Added to collection 

 16^th Oct 2008)

Virus ID: 90-44159-57

Tube labelled:

90-44159-57; YSD BHK/5 - CPAE1 - BHK2

3-12-08

(sample prepared

12th Mar 2008)

E1/BHK5/

CPAE1/BHK2

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

Reagent also available from:

USDA, Aphis, VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA

nvsluf@aphis.usda.gov

AUS1986/01

BTV

Type 3

Australia, Northern Territories 

1986

(G. Gard)

Rows I1-J10

(DPP973,

8805-25-1000)

(E1/BHK3)

[IAHBHK3]

Dr. Ian Pritchard

AAHL Geelong,

Australia

*MTA* , not to be supplied 

 to 3rd parties without written 

consent from original source

 NIG1982/06

BTV

Type 3

Nigeria (26/08/82)

NK

(animal No. 0510) 

Rows A1-B10

 (N-348) 

E1/BHK3

[BHK-1]  

Oct 2004

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