ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Epizootic haemorrhagic disease virus (EHDV) 

in the dsRNA virus collection at  IAH Pirbright

 

*IAH dsRNA virus collection number: [Country code, year / sample number] rrrr = reference strain vvvv = vaccine strain]

MTAStrains covered by a Materials Transfer Agreement.

Please send any requests for isolates from this collection to peter.mertens@bbsrc.ac.uk

 

Reference

EHDV strains

EHDV-1

EHDV-2

EHDV-3

EHDV-4

EHDV-5

EHDV-6

EHDV-7

EHDV-8

EHDV-9

Untyped

EHDV isolates

 

Family: Reoviridae,   Genus: Orbivirus,   Species: Epizootic hemorrhagic disease virus    (EHDV) 

 *IAH dsRNA virus collection number

 

( ICTVdb isolate Accession Number)

Virus Species

& Serotype

 

 (identification method)

Place of original sample eg country town

[grid reference]

 

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

 (Date added to collection)

 (Suppliers Isolate Designation)

 Adapted to which cell types

 

 

 Passage history of sample

 

[additional passage history at IAH]

* Original sample supplied to the collection by person / institution

 

 [IAH, ISIS sample number]

Comments

 

 

 References

 &

  accession numbers  

Reference strains

EHDV-1

USA1955/01

EHDV-2

CAN1962/01

 

EHDV-2

(Ibaraki virus)

JAP1959/01

EHDV-3

NIG1967/01

EHDV-4

NIG1968/01

EHDV-5

AUS1977/01

EHDV-6

AUS1981/07

EHDV-7

AUS1981/06

EHDV-8

AUS1982/06

 

EHDV - Untyped 

(2006, 2004, 1991, 1983, unspecified date)

Untyped 2006

MOR2006/21

EHDV Confirmed by RT-PCR Seg 7

Morocco

 

Region:Gharb Town:Khenichet

 

original sample taken from cattle

on

5th Sept 2006

Cell culture supernatant

 

Isolated at Biopharma Morocco

Shelf A10

Box A10 β

Rows G1-H10

 

(added to collection on 10th April 2008)

K4

[IAH/ BHK1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

MOR2006/20

EHDV Confirmed by RT-PCR Seg 7

Morocco

 

Region: Oujda Town:Berkane

 

original sample taken from cattle

on

19th Sept 2006

Cell culture supernatant

 

Isolated at Biopharma Morocco

Shelf A10

Box A10 β

Rows E1-F10

 

(added to collection on 10th April 2008)

451/06

[IAH/ BHK1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

MOR2006/19

EHDV Confirmed by RT-PCR Seg

Morocco

 

Region: Oujda Town: Guercif

 

original sample taken from cattle

on

22nd Aug 2006

Cell culture supernatant

 

Isolated at Biopharma Morocco

Shelf A10

Box A10 β

Rows C1-D10

 

(added to collection on 10th April 2008)

G21

[IAH/ BHK1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

MOR2006/18

EHDV Confirmed by RT-PCR

Seg 7

Morocco

 

Region: Fès Town: Taounate

 

original sample taken from cattle

on

13th Sept 2006

Cell culture supernatant

 

Isolated at Biopharma Morocco

 Shelf A10

Box A10 β

Rows A1-B10

 

(added to collection on 10th April 2008)

1803

[IAH/ BHK1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

MOR2006/17

EHDV

 

Confirmed by RT-PCR Seg 7

Morocco

 

from Oujda/ Guercif

 

(sample taken 22nd Aug 2006)

Treated cattle Blood

 

Isolated at IAH Pirbright

Shelf A10

Box A10 α

Rows I1-J10

 

 (added to collection on 9th April 2008)

GUR-21

[IAH/KC1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

MOR2006/16

EHDV

 

Confirmed by RT-PCR Seg 7

Morocco

 

from Fès

 

11th Sept 2006

Treated cattle blood

 

Isolated at IAH Pirbright

Shelf A10

Box A10 α

Rows G1-H10

 

 (added to collection on 9th April 2008)

1779/3

[IAH/KC1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

MOR2006/15

EHDV

 

Confirmed by RT-PCR Seg 7

Morocco

 

from

Oujda/ Taourirt

 

(sample taken 22nd Aug 2006)

Treated cattle blood

 

Isolated at IAH Pirbright

Shelf A10

Box A10 α

Rows E1-F10

 

 (added to collection on 9th April 2008)

T71

[IAH/KC1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

MOR2006/14

EHDV

 

Confirmed by RT-PCR Seg 7

Morocco

 

from Kénitra / Khenichet

 

5th Sept 2006

Treated cattle blood

 

Isolated at IAH Pirbright

Shelf A10

Box A10 α

Rows C1-D10

 

 (added to collection on 9th April 2008)

KH-45

[IAH/KC1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

MOR2006/13

EHDV

 

Confirmed by RT-PCR Seg 7

Morocco

 

from Oujda / Berkane

 

 (sample taken 19 Sept 2006)

EDTA treated cattle blood

 

Isolated at IAH Pirbright

Shelf A10

Box A10 α

Rows A1-B10

 

 (added to collection on 9th April 2008)

451/5

[IAH/KC1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

MOR2006/04

EHDV

Identified as EHDV by Rt-PCR of Seg-7

 Morocco

 

Sefrou

 

October 2006

 

Isolated from cattle blood,

 on eggs and BSR cells in Morocco

 Shelf D3,

Box D3α,

Rows G1-H10

 

 (added to collection on 10th Nov2006)

1779

E1/5BSR/BHK1

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 (A102/06a)

Derived from

MOR2006/04

original sample

MOR2006/03

 

EHDV

Identified as EHDV by Rt-PCR of Seg-7

 

Morocco

 

Oujda

 

(sample taken  Oct 2006

 

Isolated from cattle blood,

 on eggs and BSR cells in Morocco

 

 Shelf D3

Box D3 α,

Rows E1-F10

 

 (added to collection on 10th Nov2006)

T7-1/T3-1

E1/5BSR/BHK1

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 (A102/06d)

   

MOR2006/02

 

EHDV

Identified as EHDV by Rt-PCR of Seg-7

Morocco

 

Guercif

 

(Sample taken

October 2006

Isolated from cattle blood ,

on eggs and BSR cells in Morocco

 Shelf D3

Box D3 β,

Rows A1-B10

 

 (added to collection on 10th Nov2006)

G4-1/G2-1

E1/5BSR/BHK1

 Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat

(A102/06c)

   
MOR2006/01

 

EHDV

Identified as EHDV by Rt-PCR of Seg-7

Morocco 

Fes

 

October 2006

 

Isolated from cattle blood,

 on eggs and BSR cells in Morocco

 

 Shelf D3

Box D3α,

Rows I1-J10

 

 (added to collection on 10th Nov 2006)

1803 E1/5BSR/BHK1  Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

(A102/06b)

   
Untyped 2004
MOR2004/03 EHDV

Initial typing suggested 318 or type 6

(EHDV identified by virus species (serogroup) specific ELISA and  RT-PCR - 12 +ve out of 18)

Morocco

close to the Algerian border between

Er-Rachidia and Bou Arfa

Cattle

2004

    Adapted to BHK cells

Isolated from cattle blood

Dr Mehdi El Harrak

14 clinical cases in cattle that were co-habitant with healthy sheep.

 Three mortalities  

 
Untyped 1991

ISA1991/03

EHDV

untyped

Indonesia

Cattle

(1991)

(received

13/05/2004)

(D164

(RIVS 57))

(E1/AA1/BHK6

/PP3 (vero)/BHK3)

Dr. Ian Pritchard

AAHL Geelong,

Australia

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

St George et al. 1983

Untyped 983

BAR1983/02

EHDV

untyped

(strain 318)

Bahrain

Cattle

(1983)

Shelf C6
Box C6α
Rows I1- J10

 

Added to collection on 31st May 2006

 

(BK1 BHK1 OX1 BHK4)

 

 

 

BAR1983/01

EHDV

untyped

(strain 318)

Bahrain

Cattle

(1983)

Shelf B6,

box C6α,

rows E1- F10

 

(added to collection on 22/05/2006)

 

BK1/BHK4

 

Duplicate vials from position E1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position E2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

 

Untyped and unspecified sampling date
JAP----/10 EHDV    

Shelf A11,

box A11α,

rows E1-F10

 

(added to collection

28th May 2008) 

Kawanabe Virus

525-LN

HL10

[IAH/BHK1]

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

   

UNK----/06

EHDV

 

 

Shelf A11,

box A11 δ,

rows G1-H10,

 

(Added to collection

13th Jun 2008)

SU 3907 BHK10

 

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

   
UNK----/05 EHDV    

Shelf A11,

box A11 δ,

rows E1-F10,

 

(Added to collection

13th Jun 2008)

R7/03 WB2 823-27  BHK2  

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007

   
UNK----/04 EHDV    

Shelf A11,

box A11 δ,

rows C1-D10,

 

(Added to collection

13th Jun 2008)

SU3890 BHK10  

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007

   
UNK----/03 EHDV    

Shelf A11,

box A11 δ,

rows A1-B10,

 

(Added to collection

13th Jun 2008)

NY25 E1 BHK4  

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007

   
UNK----/02 EHDV       R2/04 W1B1  BHK2  

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

   
UNK----/01 EHDV       NY 20 E1 BHK2  

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007

   

EHDV-1

 (200120001999199619951955)

2001

USA2001/03

EHDV

Type 1

Monroe, Georgia, USA

White Tailed Deer

(30/Aug/2001)

Shelf A3,

box A3β,

Positions

G1-H10

 

(received 06/Feb/2004)

(Jenny-99 )

BHK

 

(BHK1)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

USA2001/02

EHDV

Type 1

Warren County, Virginia, USA

White Tailed Deer

(24/Sep/2001)

Shelf A3,

box A3β,

 Positions

I1-J10

 

(received 06/Feb/2004)

(CC146-99)

BHK

 

(BHK1)

[IAH-BHK3]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

EHDV

USA2001/01

Type 1

Washington County, Indiana, USA

White Tailed Deer

(02/Oct/2001)

Shelf A2

Box A2α

Positions

E1-F10

 

(received 06/Feb/2004)

(CC193-01)

CPAE

 

(CPAE1)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents
2000

EHDV

USA2000/08

Type 1

Kerr, Texas,

 USA

(27/Nov/2000)

White Tailed Deer

 

 

(Kerr,

TX-72)

CPAE

 

(CPAE1)

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

EHDV

USA2000/07

Type 1

Kerr, Texas,

 USA

(27/Nov/2000)

White Tailed Deer

 

 

(Kerr,

TX-68)

CPAE

 

(CPAE1)

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

EHDV

USA2000/06

Type 1

Kerr, Texas,

 USA

 

(27/Nov/2000)

White Tailed Deer

 

 

 

(Kerr,

TX-51)

CPAE

 

(CPAE1)

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 
1999

USA1999/04

EHDV Type 1

 

(confirmed as  EHDV by ELISA)

Camp Perry, Virginia, USA

 

(15/Sep/1999)

White Tailed Deer

 

 

Shelf C6'

box C6β,

rows C1-D10

 

added to collection on 5th Jun 2006

(CC137-99)

CPAE

 

CPAE1

[IAH/BHK2]

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

» contents

USA1999/03

EHDV

Type 1

New Jersey, USA

White Tailed Deer

(21/Sep/1999)

(received 06/Feb/2004)

(CC140-99)

BHK

 

(BHK1)

Dr David Stallknecht University of Georgia, USA

USA1999/02

and

USA1999/03

 have a similar origin

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

USA1999/02

EHDV

Type 1

New Jersey, USA

White Tailed Deer

(21/Sep/1999)

(received 06/Feb/2004)

(CC140-99)

CPAE

 

(CPAE1)

Dr David Stallknecht University of Georgia, USA

USA1999/02

and

USA1999/03

 have a similar origin

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents
1996

USA1996/04

EHDV

Type 1

Jefferson County, Tennessee, USA

White Tailed Deer

(29/Aug/1996)

(received 06/Feb/2004)

(CC118-96)

BHK

 

(BHK1)

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 
1995

AUS1995/02

 

(4112479B)

EHDV

type1

Australia

Cattle

(received

13/05/2004)

 
(DPP2209 (95/724 #12)) (BHK2)

Dr. Ian Pritchard

AAHL Geelong,

Australia

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

Weir et al. (1997)
1955

USA1955/01

EHDV

Type 1

New Jersey (USA)

Shelf C5,

box C5γ,

rows A1-B10

 

(added to collection

27th Apr 2006)

(23/07/01)

Duplicate vials from position A1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position A2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

» contents

 

 Shope et al (1955) 

EHDV-2

TAW----/01

EHDV 2

 

 

Shelf A10,

box A10γ,

rows A1-B10

 

(added to collection

22th May 2008)

 

Ibaraki Virus - Taiwan 41

HL6

[IAH/BHK1]

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

JAP----/09

EHDV 2

 

 

 

Ibaraki Virus - MK5-55P

BHK5

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

JAP----/08

EHDV 2

 

 

Shelf A11,

box A11α,

rows C1-D10

 

(added to collection

28th May 2008)

Ibaraki Virus - OA-41

HL3

[IAH/BHK1]

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

JAP----/07

EHDV 2

 

 

Shelf A11,

box A11α,

rows A1-B10

 

(added to collection

28th May 2008)

Ibaraki Virus - 98-MD57

 HL7

[IAH/BHK1]

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

JAP----/06

EHDV 2

 

 

Shelf A10,

box A10γ,

rows I1-J10

 

(added to collection

22th May 2008)

Ibaraki Virus - 97-MD-70V2

HL6

[IAH BHK1]

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

JAP----/05

EHDV 2

 

 

Shelf A10,

box A10γ,

rows G1-H10

 

(added to collection

22th May 2008)

Ibaraki Virus - Hyogo

HL8[IAH BHK1]

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

JAP----/04

EHDV 2

 

 

Shelf A10,

box A10γ,

rows E1-F10

 

(added to collection

22th May 2008)

Ibaraki Virus - Imaizumi

BK3 [IAH BHK1]

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

JAP----/03

EHDV 2

 

 

Shelf A10,

box A10γ,

rows C1-D10

 

(added to collection

22th May 2008)

Ibaraki Virus - FC25/1

 BHK4

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

JAP----/02

EHDV

Type 2

Japan

 

 

 

 

NIAH, Tsukuba, Japan

 

(received 25/Mar/2004)

Supplied to collection by Simon Anthony (IAH Pirbright)

March 2007 

 

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

JAP----/01

EHDV

Type 2

Ibaraki (Japan)

 

Shelf C5,

box C5β,

rows I1-J10

 

(added to collection

27th Apr 2006)

 

adapted to

BHK cells

 

BK11/BHK13/

HVMLU3/BHK5

 

[IAH/BHK3]

(23/07/01)

Duplicate vials from position I1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position J2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

 

EHDV

USA2001/13

Type 2

Sheridan, Wyoming,

 USA

White Tailed Deer

(16/Dec/2001)

 

(W8425)

CPAE

 

(CPAE2)

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

USA2001/12

EHDV

Type 2

Park, Wyoming,

 USA

White Tailed Deer

 

(Isolated 16/Dec/2001)

Shelf D1,

box D1α,

rows E1- F10

 
(added to 
collection on 
27th July 2006)

(W8477)

adapted to CPAE

 

CPAE2

+

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not  to be supplied   to 3rd parties  without written

consent from original source

 

 Duplicate vials,  supplied to Sushila Maan (IAH Pirbright) on 7th May 2009, for analysis

EHDV

USA2001/11

Type 2

Moffat, Colorado,

 USA

Elk

(16/Dec/2001)

(received 06/Feb/2004)

(W9073)

CPAE

 

(CPAE2)

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

EHDV

USA2001/10

Type 2

Converse, Wyoming,

 USA

Mule Deer

(16/Dec/2001)

(received 06/Feb/2004)

(W10023)

CPAE

 

(CPAE2)

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

EHDV

USA2001/09

Type 2

Larimer, Colorado,

 USA

Mule Deer

(16/Dec/2001)

(received 06/Feb/2004)

(W9179)

CPAE

 

(CPAE2)

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

EHDV

USA2001/08

Type 2

Moffat, Colorado,

 USA

Elk

(16/Dec/2001)

(received 06/Feb/2004)

(W9328)

CPAE

 

(CPAE2)

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

USA2001/07

 

EHDV

Type 2

Johnson, Wyoming,  USA

White Tailed Deer

(16/Dec/2001)

Shelf C6
Box C6 β,
Rows G1-H10

Added to 
collection 
7th June 2006

(W9525)

CPAE2

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

EHDV

USA2001/06

Type 2

Hill, Montana,  USA

White Tailed Deer

(16/Dec/2001)

(received 06/Feb/2004)

(W10499)

CPAE

 

(CPAE2)

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

USA2001/05

EHDVType 2

Cherry Nebraska, USA

White Tailed Deer

(16/Dec/2001)

(received 06/Feb/2004)

(W10656)

CPAE

 

(CPAE2)

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

USA2001/04

EHDV

Type 2

Valley, Montana, USA

White Tailed Deer

(16/Dec/2001)

(received 06/Feb/2004)

(W10719)

CPAE

 

(CPAE1)

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

USA2000/05

EHDV

Type 2

 

(confirmed as  EHDV by ELISA)

Habersham County, Georgia, USA

 

(18/Sep/2000)

White Tailed Deer

 

Shelf C6,

box C6β,

rows E1-F10

 

added to collection  6th June 2006

(CC148-00)

CPAE

 

CPAE1

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA

 

(received

6th Feb 2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

USA2000/04

EHDV

Type 2

Troup County, Georgia, USA

White Tailed Deer

(03/Oct/2000)

Shelf A2

 Box A2α

Rows G1-H10

 

(received 06/Feb/2004)

(CC158-00)

CPAE

 

(CPAE2)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

EHDV

USA2000/03

Type 2

Lyon County, Kansas,  USA

White Tailed Deer

(31/Aug/2000)

Shelf D1
box D1α 
rows A1-B10
 
(added to 
collection on 
12th July 2006)

 

 

(CC136-00)

CPAE

 

CPAE1

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

USA2000/02

 

EHDV

Type 2

Mechlenberg County,

North Carolina,  USA

White Tailed Deer

 

(29/Aug/2000)

Shelf C6,
Box C6γ
Rows I1-J10

(added to 
collection 
10th July 2006)

(587-3)

CPAE1

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA

 

(received

6th Feb 2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

EHDV

USA2000/01

Type 2

Chase County, Kansas  USA

White Tailed Deer

(31/Aug/2000)

Shelf A2,

BoX A2β, Positions

 G1-H10

 

(received 06/Feb/2004)

(CC137-00)

BHK

 

(BHK2)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

EHDV

USA1999/01

Type 2

Mississippi,   USA

White Tailed Deer

(05/Nov/1999)

Shelf A2,

Box A2β,

Rows

C1-D10

 

(received 06/Feb/2004)

(195-99)

BHK

 

(BHK1)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

USA1998/07

EHDV

Type 2

Macon County, Missouri  USA

White Tailed Deer

(19/Aug/1998)

Shelf D1,

box D1β,

 rows A1-B10

 

(added to collection on 20th July 2006)

 

(CC162-98)

BHK

 

BHK1

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

EHDV

USA1998/06

Type 2

Cherokee County Kansas,  USA

White Tailed Deer

(6/Oct/1998)

Shelf A2

Box A2β

Positions

E1-F10

 

(received 06/Feb/2004)

(184-98)

CPAE

 

(CPAE1)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

USA1998/05

EHDV

Type 2

Cheriton County, Missouri, 

USA

White Tailed Deer

 

(7/Oct/1998)

Shelf C6,
Box C6 γ,
Rows E1-F10
 
(added to 
collection 
6th July 2006)

(CC185-98)

CPAE1

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA

 

(received

6th Feb 2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

USA1998/04

EHDV

 

Type 2

Henderson, Tennessee,  USA

White Tailed Deer

(20/Oct/1998)

Shelf D1,

box D1α,

 rows I1- J10

 

(added to collection on 17th July 2006)

(193-98)

CPAE

 

CPAE1

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

EHDV

 

USA1998/03

Type 2

Missouri USA

Hereford Cow

 

(12/Oct/1998)

(received 06/Feb/2004)

(cow17-98)

CPAE

 

(CPAE1)

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

EHDV

 

USA1998/02

Type 2

Wythe County, Virginia USA

White Tailed Deer

 

(17/Nov/1998)

Shelf A2

Box A2α

Positions

I1-J10

 

(received 06/Feb/2004)

(CC208-98)

CPAE

 

(CPAE2)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

EHDV

 

USA1998/01

Type 2

Washington, Arkansas USA

White tailed deer

 

(28/Oct/1998)

Shelf A2

Box A2β

Positions A1-B10

 

(received 06/Feb/2004)

(CC196-98)

CPAE

 

(CPAE1)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

EHDV

 

USA1997/01

Type 2

Louisiana, USA

White tailed deer

(20/Aug/1997)

Shelf C6, box C6γ, rows A1- B10

(added to collection on 27th June 2006)

(97-13459)

CPAE

 

CPAE3

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

USA1996/03

EHDV

Type 2

Breckinridge County, Kentucky,  USA

 

Sample collected13th Sept 1996

White Tailed Deer

 

Shelf D1

Box D1α

Positions

E1-F10

 

(added to collection on

 15th July 2006)

(CC126-96)

adapted to CPAE

 

(CPAE1)

[+IAH/BHK1]

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

» contents

 

*MTA* : not to be supplied

 to 3rd parties without written

consent from original source

 Duplicate vials, position E1(g) supplied to Sushila Maan (IAH Pirbright) on 29th April 2009, for analysis and typing

USA1996/02

EHDV

Type 2

Arkansas, 

USA

White Tailed Deer

(11/Oct/1996)

Shelf D1,

box D1β,

 rows C1-D10

 

(added to collection on 20th July 2006)

(Jen42)

CPAE

 

CPAE1

[IAH/BHK2]

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

USA1996/01

EHDV

Type 2

Shelby, Alabama USA

White Tailed Deer

(02/Oct/1996)

Shelf A2

Box A2α

Positions C1-D10

 

(received 06/Feb/2004)

(148-96A)

BHK

 

(BHK2)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

EHDV

USA1994/03

Type 2

Mississippi, USA

White Tailed Deer

(26/Sep/1994)

Shelf A2

Box A2α

Positions A1-B10

 

(received 06/Feb/2004)

(T6737)

CPAE

 

(CPAE1)

[IAH-BHK2]

Dr David Stallknecht University of Georgia, USA

» contents

*MTA* : not to be supplied to 3rd parties without written consent from

original source

 

 

USA1994/02

EHDV

Type 2

Brunswick County, North Carolina, USA

White Tailed Deer

(04/Oct/1994)

Shelf D1,

box D1α,

rows C1- D10

 

(added to collection on 11th July 2006)

(CC117-94)

CPAE

 

CPAE1

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

EHDV

USA1994/01

EHDV

Type 2

Newcastle, Delaware, USA

White Tailed Deer

(15/Sep/1994)

Shelf C6,

box C6γ,

 rows C1- D10

 

(added to collection on

4th July 2006)

(CC108-94)

CPAE

 

CPAE1

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 

USA1993/01

EHDV

Type 2

Hampshire, West Virginia,  USA

White Tailed Deer

 

(30/Sep/1993)

Shelf D1,

 box D1α,

 rows G1- H10

 

(added to collection on 17th July 2006)

(CC130-93)

CPAE

 

CPAE2

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

ISA1990/01

EHDV

Type 2

Indonesia

Cattle

(1991)

Shelf  C5,

Box C5 β,

Rows C1 to D10

 

Added to collection

29th Mar 2006

(D163)

 previous

passage history unknown

 

[IAH/BHK2]

Dr. Ian Pritchard

AAHL Geelong,

Australia

 

(received

13/05/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

St George et al. 1983

ISA1988/01

EHDV

Type 2

Indonesia

Cattle

(1991)

(received

13/05/2004)

(RIVS 70)

 (unkown)

Dr. Ian Pritchard

AAHL Geelong,

Australia

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

St George et al. 1983

AUS1980/03

 

 

 

 

(41128119)

EHDV

Type 2

Australia

Cattle

(1980)

Shelf  C5,

Box C5β,

Rows E1-F10

 

(added to collection on 11th April 2006)

(CSIRO 402)

BHK9/V1/BHK11

 

[IAH/BHK1]

Dr. Ian Pritchard

AAHL Geelong,

Australia

(received

13/05/2004)

St George et al. 1983

 

*MTA* : not to be supplied

 to 3rd parties without written

consent from original source


Duplicate vial
s, position E1(g) supplied to Sushila Maan (IAH Pirbright) on 29th April 2009, for analysis and typing

USA1980/01

EHDV

Type 2

Clarke County, Georgia,  USA

(06/Oct/1980)

White Tailed Deer

 

(16/Jun/1995)

Shelf C6, box C6β, rows

I1- J 10

 

(added to collection on 27th June 2006)

(CC96-80)

CPAE

 

CPAE2

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

 
AUS1979/05

EHDV

Type 2

Australia, Northern Territory / Queensland Sentinel Cattle

(between 1979 and 1982)

Shelf  C5,

Box C6α,

Rows C1-D10

 

(added to collection on 19th May 2006)

(CSIRO 439) BHK7/V1/BHK12 B. Eaton  & A. Gould

AAHL Geelong,

Australia

(received 1990)

Reported as serologically similar to Ibaraki virus

 

Duplicate vials from position C1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position C2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

» contents

St George et al. 1983

USA1978/01

EHDV

Type 2

Lewis, Tennessee, USA

(21/Sep/1978)

White Tailed Deer

(16/Jun/1995)

Shelf C6,
Box C6γ,
Rows G1-H10
 
(added to 
collection
6th July 2006)

(CC86-78)

CPAE1

[IAH/BHK1]

Dr David Stallknecht University of Georgia, USA

 

(received 06/Feb/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

» contents

CAN1962/01

EHDV

Type 2

Alberta (USA/Canada)

 

Shelf C5,

box C5β,

rows G1-H10

 

(added to collection

25th Apr 2006)

 

adapted to

BHK cells

 

BHK2

[IAH/BHK3]

(23/07/01)

Duplicate vials from position G1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position G2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

» contents

Ditchfield et al (1962)

JAP1959/01

EHDV

Type 2

Ibaraki, Japan

Cattle

(1959)

 

(Ibaraki virus)

(BK19/BHK13

/HumLu3/BHK6)

NIAH Japan

 

(received 1977)

 

Omori et al 1969a

Omori et al 1969b

Matumoto et al 1970

Ito et al 1973

EHDV-3

 

NIG1967/01

EHDV

Type 3

  (Nigeria)

(23/07/2001)

Shelf C5

Box C5γ,

Rows C1-D10

 

(Added to collection on

28th Apr 2006)

 22619 Ibar

adapted to BHK cells

 

 MB7/BHK3

[IAH/BHK4]

Dr Shope

Yale University,

received

05-Jul-1982

Duplicate vials from position C1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position C2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

» contents
EHDV-4

NIG1968/01

EHDV-4

(Nigeria)

(23/07/2001)

Shelf C5

Box C5γ,

Rows I1 - J10

 

(Added to collection on

3rd May 2006)

33853 Ibar

adapted to

BHK cells

 

MB8/BHK3

 [IAH/BHK4]

Dr Shope

Yale University,

received

05-Jul-1982

Duplicate vials from position I1(g) sent to Bernd Hoffmann, FLI, Germany  26th Oct 2007

 

Duplicate vials from position I2(g) sent to Hagaii Yadin, Kimron Veterinary Institute 26th Oct 2007

 

Duplicate vials  from position I4 (g) A&B were given to Sushila Maan (IAH Pirbright)  1st June 2009 

 » contents

EHDV-5
AUS1979/06

EHDV

Type 5

Australia, Northern Territory / Queensland Sentinel Cattle

(between 1979 and 1982)

Shelf C5

Box C5α,

Rows I1 - J10

 

(Added to collection on 9th Feb 2006)

(CSIRO 157)

BK4/BHK1

/M2/BHK6

 

[IAH/BHK1]  

B. Eaton  & A. Gould

AAHL Geelong,

Australia

 

(received 1990)

Duplicate vials from position I1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position I2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

» contents

St George et al. 1983

AUS1977/01

EHDV

Type 5

Australia

Cattle or  Cuilcoides brevitarsis

(1977)

Shelf C6

Box C6β,

Rows A1 - B10

 

(Added to collection on 1st Jun 2006)

(CSIRO 157)

 BHK7/BK4/BHK2

/M1/BHK4

 

(BHK9/V2/BHK3)

(IAH/ )

Dr. Ian Pritchard

AAHL Geelong,

Australia

 

(received

13/05/2004)

 

St George et al. 1983

 

*MTA* : not to be supplied

 to 3rd parties without written

consent from original source

 Duplicate vials, position A1(g) supplied to Sushila Maan (IAH Pirbright) on 29th April 2009, for analysis and typing

 

EHDV-6

MOR2006/05

EHDV

Confirmed as 
EHDV by  
by Seg 7 
RT-PCR
 
Identified as
EHDV-6  by 
Rt-PCR and 
sequence 
analysis of Seg-2
Ahfir, 
Morocco
 
(October 2006)
Cattle 
(Bovine)

(Isolated 
in eggs 
and BSR cells
in Morocco)

 

 Shelf D3,

box D3β

rows C1-D9,

 

 (added to collection on 13th Nov 2006)

(451)

isolated from blood

 

 E1/BSR3

[IAH/BHK2]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco)

 

[A113/06 1]

 NOTE: Only nine vials of glycerol stock in freezer A and B

 

Vial C1(p) A -  given to Kyriaki Nomikou (IAH Pirbright) 2nd Feb 2010 - Vial position  C1 (p)  B was relocated  to  MOR2006/05 miscellaneous Box B position J1  at the bottom of freezer A

 

 

TUR2007/07

EHDV-6

 

(typed by serotype specific RT-PCR -targetting Seg-2)

Turkey 

Fethiye- Mugla region

Sample taken 26th June 2007

Bovine Organ homogenate (lung and spleen)

Shelf A12,

box A12β,

rows A1-B10

 

(added to collection on 18th July 2008)

 

[IAH/KC1]

Serife Incoglu

 & Gulnur Kalayci,

 

Boronova Veteriner Konral de Aristirma

Izmir,

Turkey

 

[A123/08 1]

derived from:

 original sample TUR2007/07

TUR2007/06

EHDV 6

By Se7 RT-PCR and Serotype RT-PCR

Mugla, Turkey

 

Reported / sampled

9th July 2007

Cattle buffy coat

 

Isolated  directly in

KC-cells  at IAH-Pirbright

Shelf A8,

box A8 γ,

rows A1-B10

 

Added to collection 

15th Jan 2008

 

[IAH/KC1]

University Faculty Veterinary Medicine, Turkey

 

[ A162/07 11]

 

TUR2007/05

EHDV 6

By Seg7 RT-PCR and Serotype RT-PCR

Mugla.

 Turkey

 

Reported / sampled

9th July 2007

Cattle buffy coats

Put directly to KC at IAH-P

Shelf A8,

box A8 β, rows I1-J10

 

(Added to collection  12th Jan 2008)

 

[IAH/KC1]

University Faculty Veterinary Medicine, Turkey

 

[A162/07 7]

 

TUR2007/04

EHDV 6

By Seg 7 RT-PCR and Serotype RT-PCR

Mugla Turkey

 

Reported 09/07/07

Cattle buffy coats

Put directly to KC at IAH-P

Shelf A8,

box A8 β, rows G1-H10

 

(Added to collection on 10th Jan 2008)

 

KC1

University Faculty Veterinary Medicine, Turkey

 

[A162/07 6]

 

TUR2007/03

EHDV 6

By Seg 7 RT-PCR and Serotype RT-PCR

Mugla Turkey

 

Reported 09/07/07

Cattle buffy coats

Put directly to KC at IAH-Pirbright

Shelf A8,

box A8 β, rows E1-F10

 

(Added to collection on 10th Jan 2008)

 

KC1

 University Faculty Veterinary Medicine, Turkey

 

[A162/07 5]

 

TUR2007/02

EHDV 6

By Seg 7 RT-PCR and Serotype RT-PCR

Mugla Turkey

 

Reported 09/07/07

Cattle buffy coats

Put directly to KC at IAH-Pirbright

Shelf A8,

box A8 β,

rows C1-D10

 

(Added to collection on 10th Jan 2008)

 

KC1

University Faculty Veterinary Medicine, Turkey

 

[A162/ 07 4]

 

TUR2007/01

EHDV 6

By Seg 7 RT-PCR and Serotype RT-PCR

 

Mugla Turkey

 

Reported 09/07/07

 

Cattle buffy coats

Put directly to KC at IAH-Pirbright

Shelf A8,

box A8 β, rows A1-B10

 

(Added to collection on 10th Jan 2008)

 

KC1

University Faculty Veterinary Medicine, Turkey

 

[A162/07 22]

 

USA2006/09

EHDV-6

 

(Initial serum neutralisation assays (with antisera to EHDV-1 to 8) suggest that it may be EHDV-6

 -

Confirmed as EHDV-6 by RT-PCR assay targeting Seg-2 and sequence analysis of cDNAs

Iroquois County,

USA

 

(sample taken

during the fall of  2006)

From white tailed deer

 

(clinical history: oral lesions, cough, respiratory congestion)

 

Date of virus

 preparation in USA

5th Jan 2007

Shelf E4,

box E4α,

rows E1-F10

 

 

(Added to collection on  2nd Oct 2007 )

 

 

06-473906-6

 

(tube labelled: 473906-6;

isol to BHK/2;

1-5-07)

From blood

 

*CPAE1/BHK2

[IAH/ ]

 

* Note: the CPAE cell cultures used for isolation are infected with bovine viral diarrhoea (BVD) virus

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

 

EHDV-6 has  not previously been identified in the USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

 

Original isolate was submitted from the Southeastern Cooperative Wildlife Disease Study (SCWDS) Athens GA. isolate obtained from tissues samples collected in the fall of 2006 in Iroquois County IL. At SCWDS, samples were innoculated into  cattle pulmonary artery endothelial (CPAE) cells cultures. sample sent to NVSL for typing. 

USA2006/08

EHDV-6

 

(Initial serum neutralisation assays (with antisera to EHDV-1 to 8) suggest that it may be EHDV-6

 -

Confirmed as EHDV-6 by RT-PCR assay targeting Seg-2 and sequence analysis of cDNAs

Iroquois County,

USA

 

(sample taken

during the fall of  2006)

From white tailed deer

 

(clinical history: oral lesions, cough, respiratory congestion)

 

Date of virus

 preparation in USA

5th Jan 2007

 

Shelf E4,

box E4α,

rows C1-D10

 

 

Added to collection on  2nd Oct 2007 

 

 

06-473906-5

 

(tube labelled: 473906-5;

isol to BHK/2;

1-5-07)

From blood

 

*CPAE1/BHK2

[IAH/ ]

 

* Note: the CPAE cell cultures used for isolation are infected with bovine viral diarrhoea (BVD) virus

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

 

EHDV-6 has  not previously been identified in the USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

 

Original isolate was submitted from the Southeastern Cooperative Wildlife Disease Study (SCWDS) Athens GA. isolate obtained from tissues samples collected in the fall of 2006 in Iroquois County IL. At SCWDS, samples were innoculated into  cattle pulmonary artery endothelial (CPAE) cells cultures. sample sent to NVSL for typing. 

USA2006/07

EHDV-6

 

(Initial serum neutralisation assays (with antisera to EHDV-1 to 8) suggest that it may be EHDV-6

 -

Confirmed as EHDV-6 by RT-PCR assay targeting Seg-2 and sequence analysis of cDNAs

Iroquois County,

USA

 

(sample taken

during the fall of  2006)

From white tailed deer

 

Date of virus

 preparation in USA

5th Jan 2007

Shelf E4,

box E4α,

rows I1-J10

 

 

Added to collection on  2nd Oct 2007 

 

 

 

06-473906-3

 

(tube labelled: 473906-3;

isol to BHK/2;

1-5-07)

From blood

 

*CPAE1/BHK2

[IAH/ ]

 

* Note: the CPAE cell cultures used for isolation are infected with bovine viral diarrhoea (BVD) virus

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

 

EHDV-6 has  not previously been identified in the USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

 

Original isolate was submitted from the Southeastern Cooperative Wildlife Disease Study (SCWDS) Athens GA. isolate obtained from tissues samples collected in the fall of 2006 in Iroquois County IL. At SCWDS, samples were innoculated into  cattle pulmonary artery endothelial (CPAE) cells cultures. sample sent to NVSL for typing. 

USA2006/06

EHDV-6

 

(Initial serum neutralisation assays (with antisera to EHDV-1 to 8) suggest that it may be EHDV-6

 -

Confirmed as EHDV-6 by RT-PCR assay targeting Seg-2 and sequence analysis of cDNAs

Iroquois County,

USA

 

(sample taken

during the fall of  2006)

From white tailed deer

 

Date of virus

 preparation in USA

25th Jan 2007

Shelf E4,

box E4α,

rows A1-B10

 

 

Added to collection on  2nd Oct 2007 

 

06-473906-2

 

(tube labelled: 473906-2;

isol to BHK/3;

1-25-07)

From blood

 

*CPAE1/BHK3

[IAH/ ]

 

 

 

 

* Note: the CPAE cell cultures used for isolation are infected with bovine viral diarrhoea (BVD) virus

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

 

EHDV-6 has  not previously been identified in the USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

 

Original isolate was submitted from the Southeastern Cooperative Wildlife Disease Study (SCWDS) Athens GA. isolate obtained from tissues samples collected in the fall of 2006 in Iroquois County IL. At SCWDS, samples were innoculated into  cattle pulmonary artery endothelial (CPAE) cells cultures. sample sent to NVSL for typing. 

USA2006/05

EHDV-6

 

(Initial serum neutralisation assays (with antisera to EHDV-1 to 8) suggest that it may be EHDV-6

 -

Confirmed as EHDV-6 by RT-PCR assay targeting Seg-2 and sequence analysis of cDNAs

Iroquois County,

USA

 

(sample taken

during the fall of  2006)

From white tailed deer

 

Date of virus

 preparation in USA

5th Jan 2007

Shelf E4,

box E4α,

rows G1-H10

 

 

Added to collection on  2nd Oct 2007 

06-473906-1

 

(tube labelled: 473906-1;

isol to BHK/2;

1-5-07)

From blood

 

cattle/*CPAE1/BHK2

[IAH/ ]

 

* Note: the CPAE cell cultures used for isolation are infected with bovine viral diarrhoea (BVD) virus

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

 

EHDV-6 has  not previously been identified in the USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab,  PO box 844,

 Ames IA, 50010, USA.

 

Original isolate was submitted from the Southeastern Cooperative Wildlife Disease Study (SCWDS) Athens GA. isolate obtained from tissues samples collected in the fall of 2006 in Iroquois County IL. At SCWDS, samples were innoculated into  cattle pulmonary artery endothelial (CPAE) cells cultures. sample sent to NVSL for typing. 

AUS1981/07

EHDV

Type 6

Australia, Northern Territory / Queensland

Sentinel Cattle

(between 1979 and 1982)

Shelf C5,

box C5γ,

Rows  G1-H9

 

(added to  collection

12th May 2006)

(CSIRO 753)

BHK12/M3/BHK3

[IAH/BHK1]

B. Eaton  &

A. Gould

AAHL Geelong,

Australia

 

(received

1990)

Duplicate vials from position G1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position G2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

» contents

 

 

St George et al. 1983

AUS1981/04

EHDV

Type 6

Australia

Cattle or  Cuilcoides brevitarsis

(1981)

Shelf C6,

box C6β,

Rows  A1-B10

 

(added to  collection

12th May 2006)

(CSIRO753)

V3/BHK10/BHK12 /M3/BHK5

Dr. Ian Pritchard

AAHL Geelong,

Australia

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

St George et al. 1983

EHDV-7
ISR2006/09

EHDV-7

 

Confirmed

by Seg 7

RT-PCR

Israel

 

(September 2006)

Cattle

(Bovine)

 

  Isolated in eggs in Israel

 

Shelf D3,

box D3β,

rows G1-H10

 

(added to collection on 13th Nov 2006)

2033/2

Isolated from whole blood

 

E1

[IAH/BHK2]

Dr Hagai Yadin Virology Dept. Kimron Veterinary Institute

 

[A97/06 no 4]

Vial position E1P given to S. Maan (IAH Pirbright) 6th Aug 2008  spare vial  held in misc box
Duplicate vial
s, position H2(g) supplied to Sushila Maan (IAH Pirbright) on 29th April 2009, for analysis and typing

ISR2006/08

EHDV-7

 

Confirmed

 by Seg 7

RT-PCR

Israel

 

(September) 2006

Cattle

(Bovine)

 

  Isolated in eggs in Israel

Shelf D3,

box D3β,

rows E1-F10

 

 (added to collection on 13th Nov 2006)

2009/2

isolated from

washed RBC

 

E1

[IAH/BHK2]

Dr Hagai Yadin Virology Dept. Kimron Veterinary Institute

 

[A97/06 no 12]

Vial position E1P given to S. Maan (IAH Pirbright) 12th Aug 2008

spare vial  held in misc box

ISR2006/07

EHDV-7

(identified as EHDV by group specific RT-PCR)

Israel

Jordan Valley

From cattle blood

 

(egg passage in Israel)

Shelf D2

box D2β

rows G1-H10

 

(added to collection on 18th Oct 2006 )

21973/940

E1

[IAH/KC2]

Hagai Yadin

(Kimron Institute Israel)

 

A97/06 no 14 (E2)

Vial position G1P given to S. Maan (IAH Pirbright) 12th Aug 2008

spare vial  held in misc box

ISR2006/06

EHDV-7

 

(identified as EHDV by group specific RT-PCR)

Israel

Jordan Valley

From cattle blood

(egg passage in Israel)

Shelf D2

box D2β

rows E1-F10

 

(added to collection on 18th Oct 2006 )

21973/940

E1

[IAH/KC1/BHK1]

Hagai Yadin

(Kimron Institute Israel)

 

A97/06 no 14 (E2)

Vial position E1P given to S. Maan (IAH Pirbright) 12th Aug 2008

spare vial  held in misc box

ISR2006/05

EHDV-7

 

(identified as EHDV by group specific RT-PCR)

Israel

Jordan Valley

From cattle blood

 

(egg passage in Israel)

Shelf D2

box D2β

rows C1-D10

 

(added to collection on 18th Oct 2006 )

2001

E1

[IAH/BHK1]

Hagai Yadin

(Kimron Institute Israel)

 

A101/06 no 11

Vial position C1P given to S. Maan (IAH Pirbright) 12th Aug 2008

spare vial  held in misc box

ISR2006/04

EHDV-7

 

(identified as EHDV by group specific RT-PCR)

Israel

Jordan Valley

From cattle blood

 

(egg passage in Israel)

Shelf D2

box D2β

rows A1-B10

 

(added to collection on 18th Oct 2006 )

2000

E1

[IAH/BHK1]

Hagai Yadin

(Kimron Institute Israel)

 

A101/06 no 10

Vial position A1P given to S. Maan (IAH Pirbright) 12th Aug 2008

spare vial  held in misc box

ISR2006/03

EHDV-7

 

(identified as EHDV by group specific RT-PCR)

Israel

Jordan Valley

 

From cattle blood

(egg passage in Israel)

Shelf D2

box D2α

rows I1-J10

 

(added to collection on 18th Oct 2006 )

2008/01

E1

[IAH/BHK1]

Hagai Yadin

(Kimron Institute Israel)

 

A101/06 no 9

Vial position J1G given to S. Maan (IAH Pirbright) 12th Aug 2008

spare vial  held in misc box

ISR2006/02

EHDV-7

 

(identified as EHDV by group specific RT-PCR)

Israel

Jordan Valley

 

From cattle blood

 

(egg passage in Israel)

Shelf D2

box D2α

rows G1-H10

 

(added to collection on 18th Oct 2006 )

1973/9247

E1

[IAH/KC1/BHK1]

Hagai Yadin

(Kimron Institute Israel)

 

A97/06 no 13

(E1)

Vial position H1G given to S. Maan (IAH Pirbright) 12th Aug 2008

spare vial  held in misc box

ISR2006/01

EHDV-7

 

(identified as EHDV by group specific RT-PCR)

Israel

Jordan Valley

 

From cattle blood

(egg passage in Israel)

Shelf D2

box D2α

rows E1-F10

 

(added to collection on 18th Oct 2006 )

1973/9247

E1

[IAH/BHK2]

Hagai Yadin

(Kimron Institute Israel)

 

A97/06 no 13

(E1)

 vial Position E10(p), was given to  Lydia Kgosana - (IAH Pirbright)

on 3rd Nov 2006

 

Vial position F1G given to S. Maan (IAH Pirbright) 12 Aug 2008

spare vial  held in misc box

AUS1981/06

EHDV

Type 7

Australia, Northern Territory / Queensland

Sentinel Cattle

(between 1979 and 1982)

 

(CSIRO 775)

(AA3/BHK19)

B. Eaton  &

A. Gould

AAHL Geelong,

Australia

 

(received

1990)

 

» contents

 

St George et al. 1983

AUS1981/05

EHDV

Type7

Australia

Cattle

(1981)

Shelf C5,

box C5γ,

rows E1-F10

 

(added to collection

28th Apr 2006)

(CSIRO 775)

AA3/BHK19

[IAH/BHK2]

Dr. Ian Pritchard

AAHL Geelong,

Australia

 

(received

13/05/2004)

Duplicate vials from position I1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position J2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

St George et al. 1983

EHDV-8

AUS1982/06

EHDV

Type 8

Australia, Northern Territory / Queensland

Sentinel Cattle

 

(between 1979 and 1982)

Shelf C6,

Box C6α

rows G1- H10

 

(Added to collection

25th May 2006)

CPR3961A

(DPP 59)

BHK14/M4/BHK4

B. Eaton  &

A. Gould

AAHL Geelong,

Australia

 

(received

1990)

Duplicate vials from position G1(g) sent to Bernd Hoffmann, FLI, Germany

26th Oct 2007

 

Duplicate vials from position G2(g) sent to Hagaii Yadin, Kimron Veterinary Institute

26th Oct 2007

» contents

 

St George et al. 1983

AUS1982/05

EHDV

Type 8

Aus

Cattle

(1982)

Shelf C5,

Box C5β

rowsA1-A10 (g)

rows B1-B10 (p)

 

(Added to collection 16th Feb 2006)

(DPP 0059)

BHK19

 

[IAH/BHK2]

Dr. Ian Pritchard

AAHL Geelong,

Australia

 

(received

13/05/2004)

*MTA* : not

to be supplied

 to 3rd parties

without written

consent from

original source

St George et al. 1983

EHDV-9

ALG2006/02

EHDV-9

 

(identified as EHDV by group specific RT-PCR,

 

Identified as EHDV-9 by RT-PCR and sequence analysis of Seg-2)

Medea,

Algeria

~13th Aug 2006

From Bovine Lymph node

 

(isolated at IAH)

Shelf D2,

box D2β,

rows I1-J10,

 

(added to collection on 19th Oct 2006 )

(No 2)

[IAH/BHK1]

Dr Hafsa Madani

 

( Institiut national de la medicine veterinaire

Laboratoire central veterinaire

Algeria)

 

 [R10/06-1]

   
                   

 

 

Original virus and spare samples

Used to generate

virus strain

Storage location

Notes

MOR2006/05

Misc Box B,  Shelf E18, freezer A position J1  

EHDV-6 isolate from Morocco 2006, spare vial

MOR2006/04

Misc  box, Freezer A,

Shelf E18, Box E18α position A1/ B1

Original virus isolate:  EHDV: sample untyped  - 
2 x 1ml cryovials: tubes labelled A102/06a 1779, E1BSR5 10/10/06,   Sefrou  EHDV -

TUR2007/07

Freezer A, Misc samples box,  shelf E1, Box E18β, row C, position C2

 EHDV-6  -  A123/08 1 ref lab submission EHDV 6 from Turkey organ homogenate

ISR2006/01

Freezer A (Misc samples) box β,  shelf E18, Box E18β, row I position 1

EHDV isolate from Israel, Jordan Valley,  Spare vial

ISR2006/02

Freezer A (Misc samples) box β,  shelf E18, Box E18β, row I position 2 EHDV isolate from Israel, Jordan Valley,  Spare vial
ISR2006/03 Freezer A (Misc samples) box β,  shelf E18, Box E18β, row I position 3 EHDV isolate from Israel, Jordan Valley,  Spare vial
ISR2006/04 Freezer A (Misc samples) box β,  shelf E18, Box E18β, row I position 4 EHDV isolate from Israel, Jordan Valley,  Spare vial
ISR2006/05 Freezer A (Misc samples) box β,  shelf E18, Box E18β, row I position 5 EHDV isolate from Israel, Jordan Valley,  Spare vial
ISR2006/06 Freezer A (Misc samples) box β,  shelf E18, Box E18β, row I position 6 EHDV isolate from Israel, Jordan Valley,  Spare vial
ISR2006/07 Freezer A (Misc samples) box β,  shelf E18, Box E18β, row I position7 EHDV isolate from Israel, Jordan Valley,  Spare vial
ISR2006/08

Freezer A (Misc samples) box β,  shelf E18, Box E18β, row I position 8

EHDV isolate from Israel, Jordan Valley,  Spare vial

ISR2006/09

Freezer A (Misc samples) box β,  shelf E18, Box E18β, row I position 9

EHDV isolate from Israel, Jordan Valley,  Spare vial

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β,

row A+B , position 1

Tissue culture Supernatant

A1 B1 A93/08 1 ref lab submission   (B1 sample given to S, Maan 1st Oct 2008)

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β,

row A+B , position 2

Tissue culture Supernatant

A2 B2 A93/08 2 ref lab submission  (B2 sample given to S, Maan 1st Oct 2008)

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β,

row A+B , position 3

Tissue culture Supernatant

A3 B3 A93/08 3 ref lab submission  (B3 sample given to S, Maan 1st Oct 2008)

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β,

row A+B , position 4

Tissue culture Supernatant

A4 B4 A93/08 4 ref lab submission  (B4 sample given to S, Maan 1st Oct 2008)

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β,

row A+B , position 5

Tissue culture Supernatant

A5 B5 A93/08 5 ref lab submission  (B5 sample given to S, Maan 1st Oct 2008)

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β,

row A+B , position 6

Tissue culture Supernatant

A6 B6 A93/08 6 ref lab submission  (B6 sample given to S, Maan 1st Oct 2008)

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β,

 row A+B , position 7

Tissue culture Supernatant

A7 B7 A93/08 7 ref lab submission  (B7 sample given to S, Maan 1st Oct 2008)

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β,

row A+B , position 8

Tissue culture Supernatant

A8 B8 A93/08 8 ref lab submission  (B8 sample given to S, Maan 1st Oct 2008)

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β,

row A+B , position 9

Tissue culture Supernatant

A9 B9 A93/08 9 ref lab submission (B9 sample given to S, Maan 1st Oct 2008)

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β, row A ,

position 10

Tissue culture Supernatant

A10 A93/08 10 ref lab submission

No isolate obtained

Freezer A (Misc samples) box β,  shelf E18, Box E18β, row C ,

position 1

Tissue culture Supernatant

C1 A93/08 11 ref lab submission

 

References

 Shope, RE; MacNamara, LG and Mangold, R. (1955).  Report on the deer mortality epizootic hemorrhagic disease of deer.  New Jersey Outdoors. 6. 17-21

Ditchfield, J; Debbie, JG; Karstad, LH (1964).  Ditchfield Trans. North. Amercian Wild life conference. 29: 196-201

 

 

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(ReoID :  Edited by Peter. P. C. Mertens and  Houssam Attoui)