*IAH dsRNA virus collection number

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 (identification method)

Place of original sample eg country town

 [grid reference]

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 Adapted to which cell types 

(Passage history of sample)

[IAH passage history]

Comments

 References &  accession numbers  

RSArrrr/01

(41010B4F)

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)

 Supplied by OVI

S. Africa*

NK 

Shelf B2,

boxB2β,

rows E1-F10

(added to collection on

17th March 1982) 

(Ref. Strain)

Vero, C6/36, KC cells

(E2/BHK7)  

[IAH-BHK2]

Duplicate samples E1(g) supplied to JongChul, Jeong, Ja Yeon Agrotech Co., Ltd., 385-6, Chon Chon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea 19th Dec 2007,

Duplicate samples, box B2β- position E2(p)  supplied to Oscar R. González Llamazares

  (Avenida Párroco Pablo Díez 49-57, 24010 - Leon - Spain,  22nd Nov 2007

Vials E3 (g) A&B were sent to Massimo Plamerini, University of Glasgow Dept Veterinary Medicine  1st June 2009

Vial E5 (g) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

[S2:AJ585122,

S3: DQ186792,

S6:AJ586695]

Maan et al 2007

RSAvvvv/01

(41010D18)

BTV Type 1

 (Typed by:  SNT, Seg-2 RT-PCR & sequence analysis)

South African vaccine strain, developed at Onderstepoort Veterinary research Institute 

NK 

Shelf C1, 

box C1α,

rows I1-J10

(Added to collection

on 

14th Sept 2005

South African vaccine

[IAH/BHK3]

duplicate samples

J10 a&b (p) given to C.Batten, IAH Pirbright on 12th Oct 2007
Duplicate vials, position I1(g) supplied to Sushila Maan (IAH Pirbright) on 29th April 2009, for analysis and sequencing

>> Contents

[S2:, S6:AJ586656 ]

BTV1

BTV1

341/09-1

BTV1

BTV1

Shelf B13

Box B13 γ

Rows G1-H10

 (added to collection on 27th Nov 2009)

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 A94/09 04

BTV1

Shelf B13

Box B13 γ

Rows E1-F10

 (added to collection on 27th Nov 2009)

338/09-1

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

A94/09 03

BTV1

Sheep blood Isolated at IAH Pirbright

Shelf B13

Box B13 γ

Rows C1-D10

 (added to collection on 27th Nov 2009)

329/09-B1

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 A94/09 02

BTV1

Sheep blood Isolated at IAH Pirbright

Shelf B13

Box B13 γ

Rows A1-B10

 (added to collection on 27th Nov 2009)

329/09-B2

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

A94/09 01

BTV-1

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

 (Typed by:  Seg-2 real-time RT-PCR )

Oman from Sohar, Batnah coast in the Arabian Gulf

Sample collected 27^th April 2009

Sheep blood

Isolated through KC cells at IAH-P

Shelf B9

Box B9 BETA

Rows  E1- F10

Oman designation F1114

Masha Japhet,

Oman

Samples arrived

Approx 15^TH May 2009

IAH Stars

number

A42/09 41

BTV-1

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

(Typed by:  Seg-2 real-time RT-PCR )

Oman from Sohar, Batnah coast in the Arabian Gulf

Sheep blood

Isolated through KC cells at IAH-P

Shelf B9

Box B9 BETA

Masha Japhet,

Oman

Samples arrived

Approx 15^TH May 2009

IAH Stars No. 

BTV-1

Identified as BTV by Seg-7 specific

Oman from Sohar, Batnah coast in the Arabian Gulf

Sheep blood

Isolated in KC cells at IAH-P

Shelf B9

Box B9 alpha

Masha Japhet,

Oman

Samples arrived

15^TH May 2009

IAH Stars No.

BTV-1

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

(Typed by:  Seg-2 real-time RT-PCR )

Oman from Sohar, Batnah coast in the Arabian Gulf

Sheep blood

Isolated in KC cells at IAH-P

Shelf B9

Box B9 alpha

BTV-1

Identified by BTV group-specific

RT-PCR 

 [3]

Typed by

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

Algeria,

Laghouat

Dakhla

(Lat: 33,849

Long: 2,834)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal No:

 TALEB MOHAMED 05

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

Algeria,

Laghouat

Dakhla

(Lat: 33,849

Long: 2,834)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal No: TALEB MOHAMED 05

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

Algeria,

Laghouat

Dakhla

(Lat: 33,849

Long: 2,834)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal No: SAHRAOUI  DJAMEL 04

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

conventional RT-PCR and specific real-time PCR 

Confirmed by
Seg- 2 sequencing

Algeria,

Laghouat

Dakhla

(Lat: 33,849

Long: 2,834)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal No: BELBOUL MOHAMED  03

Algeria,

Laghouat

Dakhla

(Lat: 33,849

Long: 2,834)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal No

BELBOUL MOHAMED  02

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

Algeria,

Laghouat

Dakhla

(Lat: 33,849

Long: 2,834)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal

No: NIA SAADI  01

Algeria,

Laghouat

Dakhla

(Lat: 33,849

Long: 2,834)

 Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal

No: NIA SAADI  01

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

Algeria

M'Sila, 

Ain Fares

Ouled Attia

(Lat: 35,74

Long: 4,531)

 Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal

No 28/304

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

Algeria

M'Sila, 

Ain Fares

Ouled Attia

(Lat: 35,74

Long: 4,531)

 Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal

No 28/302

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

Algeria

M'Sila, 

Ain Fares

Ouled Attia

(Lat: 35,74

Long: 4,531)

 Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright 

Animal

No 28/302

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

conventional RT-PCR and specific real-time PCR 

Confirmed by
Seg- 2 sequencing

Algeria

M'Sila, 

Ain Fares

Ouled Attia

(Lat: 35,74

Long: 4,531)

 Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

BTV-1Identified by BTV group-specific

RT-PCR  [3]

Typed by

conventional RT-PCR and specific real-time PCR 

Confirmed by
Seg- 2 sequencing

Algeria

M'Sila, 

Ain Fares

Ouled Attia

(Lat: 35,74

Long: 4,531)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal

No 28/300

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

Algeria

M'Sila,

Ain Fares

Ouled Attia

(Lat: 35,74

Long: 4,531)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal

No 28/300

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

Algeria

M'Sila,

Khoubana

Maadhar

(Lat: 35,74

Long: 4,531)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal No 5283/004

BTV-1

Identified by BTV group-specific

RT-PCR  [3]

Typed by

conventional RT-PCR and specific real-time PCR 

Confirmed by
Seg- 2 sequencing

Algeria

M'Sila,

Khoubana

Maadhar

(Lat: 35,74

Long: 4,531)

Outbreak 

started 

Isolated from 

whole sheep 

blood at 

IAH Pirbright

Animal No 5283/002

Hafsa MADANI, 

Ministere de 

L’Agriculture et 

du Developpement 

Rural, Institiut 

National de la 

Medicine Veterinaire

[A 172/08  02]

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 [2]

Gibraltar

Isolated from adult, male sheep blood.

(sample taken  5th Oct 2007)

(added to

collection on

  20th May 2009)

Mark Pizarro

 (Vet)

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2)

Gibraltar

Isolated from adult, female goat blood.

 October 2007

Shelf: E6

Box E6 Gamma

Rows C1-D10

Mark Pizarro (Vet)

[A111/07 11]

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2)

Gibraltar

Isolated from adult, female goat blood.

 October 2007

Shelf: E6

Box E6 Gamma

Rows A1-B10

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2)

Gibraltar

Isolated from adult, female goat blood.

(sample taken  15th Oct 2007)

 October 2007

Shelf E6,

box E6 α,

rows E1-F10

(added to

collection on

  29th Oct 2007)

From same sample as GIB2007/04

GIB2007/02

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2)

Gibraltar

Isolated from adult, female  sheep blood.

(sample taken  5th Oct 2007)

 Virus isolated at IAH Pirbright October 2007

Shelf E6,

box E6 α,

rows  C1-D10

(added to

collection on

  29th Oct 2007)

GIB2

[IAH/KC1]

outbreak data

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2)

Gibraltar

Isolated from adult, male sheep blood.

(sample taken  5th Oct 2007)

Shelf E6,

box E6 α,

rows A1-B10

(added to

collection on

  29th Oct 2007)

MOR2007/04

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2),  and Seg-2 sequence analyses (4)

Morocco

 (Blood sample taken from sheep

2nd July 2007)

Heparin treated sheep  Blood

(isolated at IAH Pirbright)

 Shelf A9,

box A9 α,

rows G1-H10

 (added to

collection on

12th March 2008)

46/07

Foci 2007

[IAH/KC1]

MOR2007/03

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2),  and Seg-2 sequence analyses (4)

Rabat,

Morocco

 (Blood sample taken from sheep

2nd July 2007)

EDTA treated

sheep blood

(isolated at IAH Pirbright)

 Shelf A9,

box A9 α,

rows A1-B10

 (added to

collection on 10th Mar 2008)

DR12/07

Foci 2007

[IAH/KC1]

MOR2007/02

BTV-1

Identified as  BTV-1  by type-specific RT-PCR targeting Seg-2 (2),  and Seg-2 sequence analyses (4)

Settat,

Morocco

 (Blood sample taken from sheep

19th July 2007)

EDTA treated

sheep blood

(isolated at IAH Pirbright)

 Shelf A9,

box A9 α,

rows C1-D10

  (added to collection on

10th Mar 2008)

SETT/07

Foci 2007

[IAH/KC1]

MOR2007/01

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2),  and Seg-2 sequence analyses (4)

Khouribga,

Morocco

 (Blood sample taken from sheep

 2nd July 2007)

Heparin Blood

(isolated at IAH Pirbright)

 Shelf A9,

box A9 α,

rows E1-F10

  (added to collection on

 10th Mar 2008)

02/07

Foci 2007

[IAH/KC1]

Sample E1 given to Anna Swain IAH on 16th May 2008 Spare single sample stored as

Misc MOR2007/01

SPA2007/03

BTV-1

Typed At LCV, Algete, Spain

Typed by real time RT-PCR

Extremadura

Spain

Sample taken

September 2007

From cattle blood

Shelf A11

Box A11 β,

E1-F10

Added to

collection on

11th June 2008

E1/C636-1

/BHK2

 [+IAH/BHK1]

derived from: original isolate

SPA2007/03

SPA2007/02

BTV-1

Typed At LCV, Algete, Spain

Typed by real time RT-PCR

Castilla –La Mancha

Spain

Sample taken

October 2007

From cattle blood

Shelf A11

Box A11 β,

C1-D10

Added to

collection on

11th June 2008

E1/C636-1

/BHK2

 [+IAH/BHK1]

derived from: original isolate

SPA2007/02

SPA2007/01

BTV-1

Typed At LCV, Algete, Spain

Typed by real time RT-PCR

and sequence analysis of 400bp of Seg-2

Andalucia Spain

Sample taken

July 2007

From sheep blood

Shelf A11,

box A11 β,

rows A1-B10

Added to

collection on

11th June 2008

E1/C636-1

/BHK2

 [+IAH/BHK1]

derived from: original isolate

SPA2007/01

TUN2007/01

BTV-1

Identified as BTV by  rt-RT-PCR targeting Seg-1 (3)

From Sheep blood

Tunisia

2007

Isolated at IAH Pirbright 18th Jan 2008

Shelf A8

box A8 α,  

Rows I1-J10

Added to

collection on

25th Jan 2008

[IAH/KC1]

Sample I1 given to Anna Swain IAH on 16th May 2008. Spare single sample stored as

Misc TUN2007/01

Outbreak data

LIB2007/08

BTV-1

identified as BTV by real time RT-PCR (Seg 1) (3)

Identified as type 1 by serotype specific

RT-PCR (Seg -2) (2)

Green Mountain, East Libya

sample collected

2nd Aug 2007

EDTA treated lysed sheep  blood

virus isolated at the CRL IAH Pirbright  October 2007

Shelf E5,

 box E5 β,

rows G1- H10

Added to collection on 15th Oct 2007

C4

[KC1/BHK1]

LIB2007/07

BTV-1

identified as BTV by real time RT-PCR (Seg 1) (3)

Identified as type 1 by serotype specific

RT-PCR (Seg -2) (2)

Green Mountain, East Libya

02/08/2007

Ovine lysed EDTA blood Isolated through KC cells at CRL

helf E5 box E5 BETA positions E1-F10

ADDED 15/10/07

C1

KC1/BHK1

LIB2007/06

BTV-1

identified as BTV by real time RT-PCR (Seg 1)  (3)

Identified as type 1 by serotype specific

RT-PCR (Seg -2) (2)

Green Mountain, East Libya

02/08/2007

Ovine lysed EDTA blood Isolated through KC cells at CRL

Shelf E5 box E5 BETA positions C1- D9 NO D10 (PDAM)

ADDED 15/10/07

B1

KC1/BHK1

LIB2007/05

BTV-1

identified as BTV by real time RT-PCR

(Seg 1)

 (Shaw et al 2007)

Identified as type 1 by serotype specific

RT-PCR

(Seg -2)(Mertens et al 2007)

Green Mountain, East Libya

02/08/200

Ovine lysed EDTA blood

Isolated through KC cells at CRL

Shelf E5 box E5 BETA positions A1- B10

ADDED 15/10/07

A3

KC1/BHK1

LIB2007/04

identified as  BTV

by real-time

RT-PCR (Seg 1:

Shaw et al 2007)

Green Mountain,

East Libya

Collected on 2nd Aug 2007

lysed EDTA treated sheep  blood

Isolated in

 KC cells

at CRL - IAH Pirbright

Shelf E2,

 box E2 γ,

 rows  G1-8 H1-8

Added to collection on 9th Oct 2007

C4

KC1

LIB2007/03

identified as  BTV

by real-time

RT-PCR (Seg 1:

Shaw et al 2007)

Green Mountain,

East Libya

Collected on 2nd Aug 2007

lysed EDTA treated sheep  blood

Isolated in

 KC cells

at CRL - IAH Pirbright

Shelf E2,

 box E2 α,

 rows  I1-8 J1-8

Added to collection on 9th Oct 2007

C1

KC1

LIB2007/02

identified as  BTV

by real-time

RT-PCR (Seg 1:

Shaw et al 2007)

Green Mountain,

East Libya

Collected on 2nd Aug 2007

lysed EDTA treated sheep  blood

Isolated in

 KC cells

at CRL - IAH Pirbright

Shelf E2,

 box E2 α,

 rows  G1-8 H1-8

Added to collection on 9th Oct 2007

B1

KC1

identified as  BTV

by real-time

RT-PCR (Seg 1:

Shaw et al 2007)

Green Mountain,

East Libya

Collected on 2nd Aug 2007

lysed EDTA treated sheep  blood

Isolated in

 KC cells

at CRL - IAH Pirbright

Shelf E2,

 box E2 α,

 rows E1-8 F1-8

Added to collection on 9th Oct 2007

A3

[IAH/KC1]

BTV1

Algeria
Berrouaghia

23rd/24th 

Aug 2006

Isolated from 

whole sheep 

blood at 

IAH Pirbright

 Shelf D2,
box D2 γ
rows G1-H10

 (added to collection

9th Nov 2006)

No10

[KC2/BHK1]

related to sample ALG2006/04

ALG2006/05

BTV Type 1

Algeria
El Bayadh

23rd-24th 

Aug 2006

Isolated from 

whole sheep 

blood at 

IAH Pirbright

 Shelf D2,
box D2 γ
rows C1-D10

 (added to collection

9th Nov 2006)

No32/ov5

[IAH/ KC2/BHK1]

BTV Type 1

Algeria
Berrouaghia

23rd/24th

Aug 2006

Isolated from 

whole sheep 

blood at 

IAH Pirbright

  Shelf D2,
box D2 γ
rows E1-F10

 (added to collection

9th Nov 2006)

No10

[IAH/KC2]

related to sample ALG2006/06

ALG2006/03

BTV Type 1

Algeria

El Bayadh

Sample taken

Whole sheep blood

 Shelf D2

Box D2 gamma

Rows I1-J10

 (added to collection on 10th Nov 2006)

No32/ov5

[IAH/KC2]

ALG2006/01

BTV Type 1

 (Identified as BTV by Seg-7 Group specific RT-PCR,

 Typed by:  , Seg-2 RT-PCR)

Berrouaghia

Media

Algeria

Isolated from Sheep spleen at IAH Pirbright ,

Isolated at IAH Pirbright  by inoculation into ECE  on 24th September 2006

(no 3)

 [IAH/E1/BHK2]

Duplicate samples, box D1γ-

position E1(p)

supplied to Concepcion

Gomez-Tejedor  (Madrid)

30th Oct 2006

Duplicate samples, box D1γ- position F1

supplied to Giovanni Savini (IZS Teramo,  4th Dec 2006)

Single sample, box D1γ- position E2 (pdam)

supplied to Miguel Ángel Jiménez C.I.S.A. Algete valdeolmos Madrid, Spain  25th May 2007- second vial kept as misc.)

Duplicate samples, box D1γ-

position E3(p)

supplied to Carolin Schumacher (Merial Lyon 22nd Nov 2007)

 Sample F2 given to Anna Swain IAH on 16th May 2008 (Duplicate tube left in Shelf E1, Box E1β)

Spare single sample

 stored as

Misc ALG2006/01

Link to outbreak data

MOR2006/12

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2),  and Seg-2 sequence analyses (4)

Oujda, 

Morocco

 (Sample taken from sheep

8th Sept 2006)

Originally isolated in Morocco on BHK cell cultures

(used to infect BHK cells at Pirbright on  18th Feb2008)

Shelf A9,

box A9 β,

rows I1-J10

 (added to

collection on

13th March 2008)

OV4/5B

Foci 2006

BHK1

[IAH/BHK1]

MOR2006/11

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2),  and Seg-2 sequence analyses (4)

Fès,

Morocco

(Sample taken from sheep 27th Oct 2006)

Originally isolated in Morocco on BHK cell cultures

(used to infect BHK cells at Pirbright on  18th Feb 2008)

Shelf A9,

box A9 β,

rows G1-H10

 (added to

collection on

13th March 2008)

ZA3

Foci 2006

BHK1

[IAH/BHK1]

MOR2006/10

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2),  and Seg-2 sequence analyses (4)

Oujda, 

Morocco

 (Sample taken from sheep

9th Oct 2007)

Originally isolated in Morocco on BHK cell cultures

(used to infect BHK cells at Pirbright on  18th Feb2008)

Shelf A9,

box A9 β,

rows E1-F10

 (added to

collection on

13th March 2008)

509/06

Foci 2006

BHK1

[IAH/BHK1]

MOR2006/09

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2),  and Seg-2 sequence analyses (4)

Oujda, 

Morocco

 (Sample taken from sheep

9th Oct 2007)

Originally isolated in Morocco on BHK cell cultures

(used to infect BHK cells at Pirbright on  18th Feb2008)

Shelf A9,

box A9 β,

rows C1-D10

 (added to

collection on

13th March 2008)

505/06 Foci 2006

BHK1

[IAH/BHK1]

MOR2006/08

BTV-1

Identified as BTV-1  by type-specific RT-PCR targeting Seg-2 (2),  and Seg-2 sequence analyses (4)

Oujda, 

Morocco

 (Sample taken from sheep

9th Oct 2007)

EDTA

Blood

(isolated in Morocco)

 Shelf A9,

box A9 α,

rows I1-J10

 (added to

collection on

12th March 2008)

Ham

Foci 2006

BHK1

 [IAH/BHK1]

MOR2006/07

BTV Type 1

 (Identified as BTV by Seg-7 Group specific RT-PCR,

Typed by  Seg-2 RT-PCR & sequence analysis)

Isolated from a small village called Guenfouda near Oujda in the East of Morocco.

Isolated from a Sheep showing clinical BT in Morocco, using embyonated chicken eggs  and BSR cells

 Shelf D3

Box D3 ALPHA

Rows C1-D10

 (added to

collection on

10th Nov 2006 )

1- OV4 - passage 1E3BSR

(A112/06

No 2)

E1/BSR4

[IAH/BHK2]

Related to sample MOR2006/06

BTV Type 1

 (Identified as BTV by Seg-7 Group specific RT-PCR,

 Typed by:  , Seg-2 RT-PCR)

(sample taken Oct 2006)

Isolated from a Sheep showing clinical BT in Morocco, using embyonated chicken eggs  and BSR cells

1- OV4 - passage 1E3BSR

(A112/06

No 1)

E1/BSR4

[IAH/BHK2]

Related to sample MOR2006/07

Duplicate samples, box D3α, position B10(g), supplied to

Giovanni Savini, IZS Terramo, Italy

 - 4th Dec 2006 

Duplicate samples, box D3α-

position A1(p)

supplied to Carolin Schumacher (Merial Lyon 22nd Nov 2007)

USA2004/02

BTV- 1

Virus serotype identified by RT-PCR using type specific primers and sequencing (Seg-2) at SCWDS .

USA: St. Mary Parish Louisiana

Sample taken in the Fall of  2004

White tailed deer

A deer was observed by a hunter to be staggering and was killed /tissues collected.

Isolated at the Southeastern Cooperative Wildlife Disease Study (SCWDS) Athens GA.

Shelf A16,

box A16 α,

rows C1-D10

(Added to collection 

 16^th Oct 2008)

Virus ID: 05-350924

Tube labelled: 350924: BHK/2 from re-isolation

(sample preparation date not recorded)

Isolated from tissue sample

E1/BHK2

Technical contact in USA: Donna Johnson

:

 Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844 Ames IA, 50010 USA.

(82% similar (Seg-2) to other BTV-3 isolates from Florida (1130 bp region))

IND2003/10

BTV

Type 1

 (Typed by

Seg-2 RT-PCR

/ sequence analysis)

India

From blood

Shelf D4,

box D4 γ,

rows I1-J10

(added to

collection on

24th Nov 2006) 

[+IAH/BHK3 ]

Sample given to Sushila maan IAH Pirbright Sept 2006 for sequencing studies

IND2003/05

BTV

Type 1

 (Typed by SNT & by Seg-6 RT-PCR / sequence analysis)

Tamil Nadu, India

Sheep

(Isolated at Tamil Nadu Veterinary And Animal Sciences University, Chennai-600 051 (India))

(125)

BHK cells

(BHK5)

[IAH/E1/BHK2]

[S6: AJ783903]

IND2003/04

BTV

Type 1

 (Typed by SNT & by Seg-6 RT-PCR / sequence analysis)

Sheep

(Isolated at Tamil Nadu Veterinary And Animal Sciences University, Chennai-600 051 (India))

shelf B5,

box B5α

rowsA1-B10

added to collection on 8th July 2005

(122)

BHK cells

(BHK5)

[IAH/E1/BHK2]

[S6:AJ783902]

GRE2001/11

BTV Type 1

(identified as BTV by ELISA and Seg-7 RT-PCR [3]  :

 Typed by serum neutralisation assay and seg 2 RT-PCR [2])

GREECE , Karies-Zagoriou-Miliotades Ioannina (NW)

(sample

taken

10th Sept2001)

Sheep (blood)

isolated at: Centre of Athens Vet institutions, virus lab,  by  K.Nomikou

Shelf A12,

box A12 α,

rows I1-H10

(Added to

collection

18th July 2008  

animal

code 1

Blood to BHK 21 CELLS

 BHK 9

[+IAH/BHK1

GRE2001/10

BTV-1

(identified as BTV by ELISA and Seg-7 RT-PCR

  :

 Typed by serum neutralisation assay

&

Seg 2 RT-PCR)

GREECE , Thesprotia village: Potamia-Paraminthia

(west-mainland Greece) 

( sample taken 16^th Nov 2001)

Sheep (spleen)

At: Centre of Athens Vet institutions, virus lab  

 K.Nomikou and

O. Mangan

isolated

3rd Dec2001

Shelf A12,

box A12 α,

rows D1-E10

 Added to

collection on 

14th July 2008

from spleen, to

 BHK 21 CELLS

E1 BHK 6

[+IAH/BHK1]

GRE2001/09

BTV Type 1

(identified as BTV by ELISA and Seg-7 RT-PCR 

:

 Typed by serum neutralisation assay

&

seg 2 RT-PCR)

GREECE , Lesbos

Agiasos-Vasilika,

Central island

Animal code 295

(sample taken 17th Oct 2001)

Sheep (BLOOD)

Isolated on 24th Nov 2001

at the Centre of Athens Vet institutions, virus lab  by K. Nomikou

Shelf A12

Box A12 α,

rows B1-C10

 Added to

collection on 

14th July 2008

from blood to

 BHK 21 CELLS

 BHK 6

[+IAH/BHK1]

GRE2001/08

BTV Type 1

(identified as BTV by ELISA and Seg-7 RT-PCR  :

 Typed by serum neutralisation assay and seg 2 RT-PCR)

Greece, Lesbos

Agiasos-Achladeri,

Central island

(sample taken 15th Oct 2001)

isolated from Sheep (spleen)

Isolated at the Centre of Athens Vet institutions, virus lab, by:  K. Nomikou

(Isolated on 1st Nov2001)

Shelf A11

Box A11 γ,

rows I1-J10

(Added to

collection

10th July 2008 

Animal code 280

Spleen to BHK 21 CELLS

 BHK 3

[+IAH/BHK1]

GRE2001/07

BTV

Type 1

(Identified as BTV by ELISA & RT-PCR,

 (Typed by SNT & by Seg-2 RT-PCR)

 Lesbos Agiasos-Achladeri (Central island)

GREECE

[39^o 05΄Ν

26^o 09΄Ε]

 (15/Oct/2001)

From sheep

(animal code 265)

 (Isolated at: Centre of Athens Vet. Institutions, Virus Lab, Athens by:

Dr. Nomikou Kiki

1-11-200 )

shelf B5,

box B5α

rows I1-J10

sample received

19th Oct. 2004

GR1472/01 LS (265)

BHK 21 cells

from blood

Seg 10 sequenced at E1/BHK5

Isolate used by Shujing Rao, IAH Pirbright for cDNA synthesis, to send to P.Roy for VLP production (March 2005)

Aliquots 1/10a & b (p) given to Eva Veronesi (IAH Pirbright)  27th Sept 2005

[Seg3:DQ186807

Seg10:AY677628]

GRE2001/06

BTV Type 1

Identified as BTV by ELISA & RT-PCR, Typed by serum neutralisation assay

Smiksi,

Grevena,

Greece

 (NW)

[40^o 04΄ Ν,

21^o 07΄Ε]

 (31/Dec/2001)

Sheep (spleen)

At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana

30th Sept. 2004

GR15/01GRES

BHK 21 cells

spleen

BHK8

[IAH/BHK2]

Seg-10 sequenced at BHK10

[Seg3:DQ186820

Seg10:AY449657]

GRE2001/05

BTV Type 1

Identified as BTV by ELISA & RT-PCR, Typed by serum neutralisation assay

Oraiokastro - Delvinaki,

Ioannina, Greece

 (N.W)

[39^o 59΄Ν,

20^o 33΄Ε]

(18/ Sept/ 2001

Sheep (spleen)

 At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana

Shelf B4,

Box B4β

Row A1- B10

Added to collection on 30th Sept. 2004

GR1245/01 IOS

BHK 21 cells

Spleen

(E1BHK9)

[IAH/BHK2]

[Seg3:DQ186822 ]

GRE2001/04

BTV Type 1

 (Identified as BTV by:

 ELISA , SNT & RT-PCR. Serotyped by: SNT)

Greece Lesbos Agiasos-Achladeri  (Central island)  Animal code:280

[39^o 05΄Ν,

26^o 09΄Ε]

 (15/Oct/2001)

From sheep

  (01/Nov/2001 At:Centre of Athens Vet. Institutions, Virus Lab. By: Dr. Nomikou Kiki)

RowsG1-H10

 (20/May/2003)

GR1472(2)/01 LS  (280)

 spleen

BHK 21 cells

 ( BHK3 )

[IAH-BHK2]

Isolated from the same location (same farm) as GRE2001/03 (GR1472(1)/01LS)

Tubes

 1/10(g)-G1

&

1/10(p)-H1 supplied to

S. Anthony

&

H. Jones,

 IAH Pirbright

 07-06-2004

GRE2001/03

BTV  Type 1

(Identified as BTV by:

 ELISA , SNT & RT-PCR. Serotyped by: SNT)

Greece Lesbos Agiasos-Achladeri  (Central island)  Animal code:280

[39^o 05΄Ν,

26^o 09΄Ε]

 (15/Oct/2001) 

From sheep

  (01/Nov/2001 At:Centre of Athens Vet. Institutions, Virus Lab. By: Dr. Nomikou Kiki)

Rows I1-J10

 (20/May/2003)

GR1472(1)/01LS (280)

 spleen

BHK 21 cells

 ( BHK3 )

[IAH-BHK2]

Isolated from the same location (same farm) as GRE2001/04

(GR1472(2)/01LS)

Tubes

 1/10(g)-I1

&

1/10(p)-J1

 supplied to

S. Anthony

&

H. Jones,

 IAH Pirbright

 07-06-2004 

>> Contents

GRE2001/02

BTV Type 1

 (Typed by: SNT & Seg-2 RT-PCR plus sequencing)

Greece

Lesbos

[39^o 05΄Ν,

26^o 09΄Ε]

(2001)

Sheep

21/12/2001

Shelf A5,

Box A5γ,

positions

G1-H10

GR-1472/01 LS

spleen

BHK 21 cells

[IAH-BHK3] 

[S10: ]

GRE2001/01

(41002BEB)

BTV Type 1

 (Typed by: SNT & Seg-2 RT-PCR plus sequencing)

Greece,

 Lesbos

[39^o 05΄Ν,

26^o 09΄Ε]

 (2001)

Sheep

 26/12/2001

Shelf A5,

Box A5γ,

positions

E1-F10

 (01/Apr/2004) 

GR-1472/01 LS

blood

BHK 21 cells

[IAH-BHK3]

Samples (p) 1/10a

and (p) 1/10b,

given to Adam Meyer (IAH Pirbright) for virus growth and RNA production for Raniero Lorenzetti

(June 2005)

Sample E1 given to Anna Swain IAH on 16th May 2008, spare single sample stored as

Misc GRE2001/01

Vial positions  E2 (g)  were given to Eva Veronesi (IAH Pirbright on 6th Nov 2009 for Ph.D studies).  One of these   was retained as back up, in  the 'miscellaneous' box D18α, position H1 at the bottom of freezer A

>> Contents

 [S2, S6:AJ586664 S7, S8, S9, S10] 

IND2001/01 

(41002A82)

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)

India (Chennai.) (19/Feb/2001)

Culicoides 

Shelf B2, boxB2α,

rows I1-J10

(16/Mar/2005)

 (INDe )

BHK cells

 (BHK6)

[IAH-BHK3]

Sample Nos. 1/10g(a+b)  were given to Natalie Ross-Smith (IAH Pirbright) for sequencing studies,

 7th March 2006

---------------------------

Duplicate samples

I2(g) supplied on 19th Dec 2007, to JongChul, Jeong, Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

>> Contents

[Seg2:

Seg3:DQ186811

 Seg6:AJ586663]

IND1999/01 

(41002921)

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)

India (Haryana.)

 (06/Aug/1999)

 Sheep  

Shelf B3,

box B3β,

rows A1-B10

(INDd )

BHK cells

 (E2/BHK9)

+

[IAH/BHK1]

 [S2: S6:AJ586662

S7:AM261977]

IND1992/02

(410025A0)

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)  

India

 (03/Nov/1992)

NK  

Shelf C1, 

box C1α,

rows G1-H10

Added to 

collection

14th Sept 2005

(INDB)

 BHK cells

E1/BHK10

[IAH-BHK2]

 [S2: S6:AJ586660 ]

IND1992/01

(4100244C)

BTV  Type 1

identified as BTV by immuno-fluorescence, and immunoperoxidase tests

(Type confirmed by serology and Seg-2 RT-PCR and sequencing)

India

(Rajasthan)

 (Isolate from sample taken in Avikanagar in Rajasthan State, India

3rd March 1992)

Isolated from blood of sheep showing typical bluetongue symptoms

In India by

Dr. Gaya Prasad

Hisar

 IND01-01

 (INDA)   

BHK

[IAH/BHK]

 >> Contents

[S2:

S6:AJ586659

S7: AM261976]

Prasad et al 1994

IND1988/01 

(4100274F)

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)

India

 (24/Sept/1988)

NK  

Shelf C1, 

box C1α,

rows E1-F10

Added to 

collection

14th Sept 2005

(INDC )

BHK cells

BHK9

[IAH-BHK2]

>> Contents

[S2: S6:AJ586661]

MAY1987/01  

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)

Kuala Lumpur (00/Apr1987)

 NK 

(added to collection on 16/08/2005)

 ( )

BHK cells

 (E1/BHK2)

[IAH/BHK3]

Duplicate samples

C1(g) supplied on 19th Dec 2007, to JongChul, Jeong, Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

 [Seg2:

Seg3:DQ186816 ]

SUD1987/01

( 4100223B ) 

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)

Sudan (16/Apr/1987)  

NK

Shelf C3, 

box C3β,

rows E1 -F10

(added to collection on 18th Nov 2005)

(NY-76 )

BHK cells

E2/BHK2/PP1

[IAH/BHK2]

 >> Contents

[S2: S6:AJ586658]

IND1985/01

BTV-1

India

 Sample taken

 in 1985

Shelf D5

Box D5 alpha

Rows E1-F10

[IAH/BHK3]

Shambat, Sudan

Sample taken in 1983

Shelf B11,

box B11γ,

rows G1-H10

NY76

BHK cells

E2/BHK2/PP1

[IAH/BHK3]

 NIG1982/01 

(40FED549)

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)

Nigeria (19/Aug/1982)   

NK 

shelf B1,

box B1α

rows E1-F10

(02/Feb/2005)

(N-423 ) 

BHK cells

(E1/BHK3)

[IAH: BHK1]

[Seg2:

Seg3:DQ186818 Seg6:AJ586657]

CAR1982/01

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)

Cameroon (29/Oct/1982)

Sheep

shelf C2, 

box C2α,

rows G1-H10

Added to 

collection on

18th Oct 2005

 (82/4)

BHK cells

 (E1/BHK1)

[IAH/BHK2]

 >> Contents

[S2:]

 AUS1981/03

BTV Type 1/6?

Australia

(5/Oct/1981)

 ( CSIRO 154 No. 34980)

 Vero, BHK cells

 (V3/BHK7)

>> Contents

AUS1981/02

BTV Type 1/6?

 Australia (7/Oct/1981))

 NK

( CSIRO 156 No. 34998)

 Vero, BHK cells

 (V4/BHK7)

AUS1981/01

(41002D85)

BTV Type 1

Australia (7/Oct/1981)

NK

( CSIRO 156 No. 34993)

Vero, BHK cells

 (V4/BHK7)

 >> Contents

[S2:

S6 AJ631214 ]

AUS1979/01

BTV type 1

Australia,

Northern Territories

1979

(T. St George)

shelf B1,

box B1α

rows I1 - J10

(02/Feb/2005)

(CS156

7906-26-0004)

(BHK3/V4/

BHK1/V1)

[IAH: BHK1]

(MTA)

Aliquots 1/10a & b (p) given to Eva Veronesi (IAH Pirbright)  27th Sept 2005

AUS----/01  

BTV  Type 1

(Type confirmed by Seg-2 RT-PCR and sequencing)

Australia 

NK 

BHK cells

[S2:]