ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 16 (BTV-16)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection » Contents

 

 

 

 Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus  type 16 (BTV-16)

 *IAH dsRNA virus collection number

 

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 

 (identification method)

Place of original sample eg country town

 [grid reference]

 

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 

 Adapted to which cell types 

 

(Passage history of sample)

 

[IAH passage history]

* Original sample supplied to the collection by person / institution

 

[IAH, ISIS sample number]

Comments

 References &  accession numbers  

Reference and vaccine strains

RSArrrr/16

BTV

Type 16

 

(Eastern topotype group)

Supplied by OVI S. Africa*

NK

Shelf B1,

box, B1γ,

rows E1-F10

 

 (10/02/83)

 (BTV-16 Reference Strain)

E2/BHK7

[IAH/BHK2]

 

Isolate originally  from Pakistan

 

Duplicate samples  E1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, Kyung Ki-Do, 440-330, Korea

 

Vial F2 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

 

» contents

 

 [S2: AJ585137,  S3: DQ186796

S5: AM773707, S6: AJ586719]

 

Maan et al 2007

RSAvvvv/16

BTV

Type 16

 

(Eastern

topotype group)

South African BTV-16

Vaccine strain

 

NK

Shelf B4,

Box B4β

Row C1- D10

 

(added to collection on 07/04/03) 

 BTV16 Vaccine strain 

batch no.1

 

 expiry date

01/12/2005 BL6096 S

[IAH/BHK1]

derived

from RSAvvv1/16

 Samples (p) 1/10a & b removed and given to Sarah Gold (IAH Pirbright)

1st Nov 2005.

 

 Sample supplied to Stephan Zientara, Maisons Alfort France  Dec 2007

 

Duplicate vials, position C1(g) supplied to Sushila Maan (IAH Pirbright) on 29th April 2009, for sequencing

» contents

 

 [S2: AJ585149

S5:  AJ586689 S6:AJ586689]

 

 Related to reference strain RSArrrr/16

RSAvvv1/16

BTV

Type 16

 

(Eastern

topotype group)

South African BTV-16

Vaccine strain

 

NK

 

BTV16 Vaccine strain 

batch no.1

 

 expiry date

01/12/2005 BL6096 S

un-passaged original  vaccine,

diluted to manufacturers instructions

 

used to generate RSAvvvv/16

&

RSAvvv2/16

 

 

RSAvvv2/16

BTV

Type 16

 

(Eastern

topotype group)

South African BTV-16

Vaccine strain

 

NK

 

 BTV16 Vaccine strain 

batch no.1

 

 expiry date

01/12/2005 BL6096 S

[IAH/BHK1]

derived

from RSAvvv1/16

   
2009

GRE2009/07

BTV-16

 

Identified by seg-2 specific Real time RT-PCR

Greece: Mandamados

Isolated  from cattle blood

 

 at

IAH-P

2nd Nov 2009

Shelf B13,

box B13 β,

rows  A1–B10

Added to collection

12th Nov 2009

No:EL83303070

Animal id: 0389

Blood sample from cattle

 

[IAH KC2]

Supplied by Olga Mangana

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A81/09 no 8]

outbreak data

GRE2009/06

BTV-16

 

Identified by seg-2 specific Real time RT-PCR

Greece: Mandamados

Isolated  from cattle blood

 

 at

IAH-P

2nd Nov 2009

Shelf B13,

box B13 α,

rows  I1–J10

Added to collection

12th Nov 2009

No:EL83303013

Animal id: 0321

Blood sample from cattle

 

[IAH KC2]

Supplied by Olga Mangana

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A81/09 no 7]

outbreak data

GRE2009/05

BTV-16

 

Identified by seg-2 specific Real time RT-PCR

Greece:

Agia Paraskevi Kalloni Herd

Isolated  from cattle blood

 

 at

IAH-P

2nd Nov 2009

Shelf B13,

box B13 α,

rows  G1–H1

Added to collection

12th Nov 2009

No:EL83306115

Animal id: 0138

Blood sample from cattle

 

[IAH KC2]

Supplied by Olga Mangana

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A81/09 no 1]

outbreak data

GRE2009/04

BTV-16

 

Identified by seg-2 specific Real time RT-PCR

Greece:

Mandamados Herd

Isolated  from cattle blood

 

 at

IAH-P

2nd Nov 2009

Shelf B13,

box B13  α,

rows  E1-F10

Added to collection

12th Nov 2009

No:EL83303555

Animal id: 0345

Blood sample from cattle

 

[IAH KC1]

Supplied by Olga Mangana

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A81/09 no 5]

outbreak data

GRE2009/03

BTV-16

 

Identified by seg-2 specific Real time RT-PCR

Greece:

Mandamados Herd

Isolated  from cattle blood

 

 at

IAH-P

2nd Nov 2009

Shelf B13,

box B13 α,

rows  C1-D10

Added to collection

12th Nov 2009

No:EL83303555

Animal id: 0112

Blood sample from cattle

 

[IAH KC1]

Supplied by Olga Mangana

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A81/09 no 4]

outbreak data

GRE2009/02

BTV-16

 

Identified by seg-2 specific Real time RT-PCR

Greece:

Mandamados Herd

Isolated  from cattle blood

 

 at

IAH-P

2nd Nov 2009

Shelf  B13,

box  B13 α,

rows - A1-B10

Added to collection

12th Nov 2009

No:EL83303555

Animal id: 0165

Blood sample from cattle

 

[IAH KC1]

Supplied by Olga Mangana

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A81/09 no 3]

outbreak data

GRE2009/01

BTV-16

 

Identified by seg-2 specific Real time RT-PCR

Greece:

Mandamados Herd

Isolated  from cattle blood

 

 at

IAH-P

2nd Nov 2009

Shelf  B12,

box B12 γ,

rows - I1-J10

Added to collection

12th Nov 2009

No:EL83303555

Animal id: 0267

Blood sample from cattle

 

[IAH KC1]

Supplied by Olga Mangana

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A81/09 no 2]

outbreak data

OMN2009/07

BTV-16

 

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

Oman from Sohar,

Batnah coast in the Arabian Gulf

Sample collected
19th Apr 2009

Sheep blood

 

Isolated in KC cells at IAH-P

Shelf B9,

box B9 β,

rows C1- D10

Added to collection on 16th Jun 2009
Oman
designation
 F274
[IAH/KC1]

Masha Japhet,

Oman

 

Samples arrived

15TH May 2009

 

[IAH Stars No.
A42/09 40]
not all +ve animals showed clinical signs of disease. Only Wiltshire sheep, Arabian gazelles, fallow deer and blackbucks were affected . Only one multi-horn sheep died, showing similar signs and post mortem changes, but all of the other live animals sampled were -ve. The +ve Cattle, buffalo, goats and reem gazelles showed no signs of disease .
Strains of BTV-1, BTV-4 and BTV-8  were also isolated in early 2009 (see:  OMN Isolates)
OMN2009/02

BTV-16

 

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

Oman from Sohar,

Batnah coast in the Arabian Gulf

Sample collected
13th Apr 2009

Sheep  blood

 

Isolated in KC cells at IAH-P

Shelf B9,

box B9α,

rows C1- D10

 

Added to collection on 16th Jun 2009

Oman
designation
F274
[IAH/KC1]

Masha Japhet,

Oman

 

Samples arrived

15TH May 2009

 

[IAH Stars No.
A42/09 20]
not all +ve animals showed clinical signs of disease. Only Wiltshire sheep, Arabian gazelles, fallow deer and blackbucks were affected . Only one multi-horn sheep died, showing similar signs and post mortem changes, but all of the other live animals sampled were -ve. The +ve Cattle, buffalo, goats and reem gazelles showed no signs of disease .
Strains of BTV-1, BTV-4 and BTV-8  were also isolated in early 2009 (see:  OMN Isolates)
ISR2009/03

BTV-16

 

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

Israel,

 from a village called: Ein Ha Tzeva 

samples

30th march 2009

ovine blood

Isolated through KC cells at IAH-P

Shelf B8,

box B8γ,

rows  I1-J10

 Added to collection
 8th June 2009

Kimron

 Institute

 number

34392 1957/3

[IAH/KC2]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 14TH April 2009

 

IAH Stars
number
A25/09 15
   
ISR2009/02

BTV-16

 

Identified as BTV by Seg-7 specific

 Real time RT-PCR

Israel,

 from a village called: Ein Ha Tzeva 

samples

30th march 2009

ovine blood

Isolated through KC cells at IAH-P

Shelf B8

box B8γ,

rows

G1-H10

Added to collection 8th June 2009

Kimron Institute

 number

 

34392 1957/2
KC2

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 14TH April 2009

 

IAH Stars
number
A25/09 14
   
ISR2009/01

BTV-16

 

Identified as BTV by Seg-7 specific

 Real time RT-PCR

Israel,

 from a village called: Ein Ha Tzeva 

samples

30th march 2009

ovine blood

Isolated through KC cells at IAH-P

Shelf B8

box B8γ,

rows E1-F10

Added to collection 8th June 2009

Kimron Institute

 number

34392 1957/1

KC2

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 14TH April 2009

 

IAH Stars
number
A25/09 13
   
2008

ISR2008/03

BTV-16

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR [3] 

 

Identified as serotype 16 by Seg-2 specific real time RT-PCR

From Israel,

 from a village called: 

Ein Kamonim

 

samples collected on

24th Oct  2008

 

From goat spleen

 

Isolated in embryonated chicken eggs in Israel

Shelf B8,

box B8 α,

rows A1-B10

 

Added to collection on  28th Jan 2009

Kimron

institute  No.

591665/6/7

 

E1

[IAH/BHK2]

 

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

~17th Dec 2008

 

[IAH Stars
No.

A181/08 3]

 

 

ISR2008/01

BTV-16

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR

[3]   

 

Identified as serotype 16 by Seg-2 specific real time RT-PCR

From Israel,

 from a village called: Rosh Ha-Nikrai

 

samples collected on 19th Nov 2008

From cattle blood.

 

Isolated in embryonated chicken eggs in Israel

Shelf B7,

box B7γ, 

rows G1-H10

 

Added to collection on  28th Jan 2009

Kimron

institute  No.

592816

E1

[IAH/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

~ 17th Dec 2008

 

[IAH Stars
No. A181/08 1]
   

GRE2008/10

BTV-16

 

Identified by seg-2 specific RT-PCR

From Greece:

Lesbos,

Madamado

 

Herd no: EL83303013

from sheep blood

 

Isolated at

IAH-P

1st Dec 2008

Shelf A18,

box A18 β,

rows G1 –H10

 

Added to collection

2nd Dec 2008

Herd no: EL83303013

 

Animal ID: EL833030130334

[IAH/KC1]

Supplied by Olga Mangana:

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A169/08 no 10]

 

 

GRE2008/09

BTV-16

 

Identified by seg-2 specific RT-PCR

From Greece:

Lesbos,

Madamado

 

Herd No: EL83303013

from sheep blood

 

Isolated at

IAH-P

1st Dec  2008

Shelf A18,

box A18 beta,

rows E1 –F10

 

Added to collection

2nd Dec 2008

 

Herd No: EL83303013

 

Animal ID: EL833030130265

[IAH/KC1]

Supplied by Olga Mangana:

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A169/08 no 9]

Note only 8 glycerol samples in freezer B

 

GRE2008/08

BTV-16

 

Identified by seg-2 specific RT-PCR

From Greece:

Lesbos,

Madamado

 

Herd No: EL83303013

from sheep blood

 

Isolated at

IAH-P

1st Dec 2008

Shelf A18,

box A18 β,

rows C1–D10

 

Added to collection

2nd Dec 2008

Herd No: EL83303013

 

Animal ID:

EL833030130333

[IAH/KC1]

Supplied by Olga Mangana:

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A169/08 no 8]

 

 

GRE2008/07

BTV-16

 

Identified by seg-2 specific RT-PCR

From Greece:

Lesbos,

Madamado

 

Herd No:

EL83303013

from sheep blood

 

Isolated at

IAH-P

1st Dec 2008

Shelf A18,

box A18β,

rows A1–B10

 

Added to collection

2nd Dec 2008

Herd No:

EL83303013

 

Animal ID:

EL833030130332

 

[IAH/KC1]

 

Supplied by Olga Mangana:

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A169/08 No. 7]

   

GRE2008/06

BTV-16

 

Identified by seg-2 specific RT-PCR

From Greece:

Lesbos,

Madamado

 

Herd No:

EL83303013

from sheep blood

 

Isolated at

IAH-P

27th Nov 2008

Shelf A18,

box A18 α,

rows I1 –J10

 

Added to collection

 29th Nov 2008

Herd No:

EL83303013

 

Animal ID: EL833030130215

 

[IAH/KC1]

Supplied by Olga Mangana:

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A169/08 no 6]

 

 

GRE2008/05

BTV-16

 

Identified by seg-2 specific RT-PCR

 

From Greece:

Lesbos,

Madamado

 

Herd No:

EL83303013

 

from sheep blood

 

 Isolated at

IAH-P

27th Nov 2008

Shelf A18,

box A18 α,

rows G1–H10

 

Added to collection

 29th Nov 2008

Herd No:

EL83303013

 

Animal ID: EL833030130263

[IAH/KC1]

Supplied by Olga Mangana:

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A169/08 no 5]

 

 

GRE2008/04

BTV-16

 

Identified by seg-2 specific RT-PCR

 

From Greece:

Lesbos,

Madamado

 

Herd No: EL83303013

from sheep blood

 

Isolated at

IAH-P

27th Nov 2008

Shelf A18,

box A18 α,

rows E1 –F10

 

Added to collection

 29th Nov 2008

Herd No: EL83303013

 

Animal ID: EL833030130153

[IAH/KC1]

Supplied by Olga Mangana:

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A169/08 no 4]

Note only 8 glycerol samples in freezer B

 

GRE2008/03

BTV-16

 

Identified by seg-2 specific RT-PCR

 

From Greece:

Lesbos,

Madamado

 

Herd No:

EL83303024

from sheep blood

 

Isolated at

IAH-P

27th Nov 2008

Shelf A18,

box A18 α,

rows C1 –D10

 

Added to collection

 29th Nov 2008

Herd No:

EL83303024

 

Animal ID: EL8330302432

 

 

[IAH/KC1]

Supplied by Olga Mangana:

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A169/08 no 3]

 

 

GRE2008/02

BTV-16

 

Identified by seg-2 specific RT-PCR

 

From Greece:

Lesbos,

Madamado

 

Herd No:EL83303024

from sheep blood

 

Isolated at

IAH-P

27th Nov 2008

Shelf A18,

box A18 α,

rows A1 –B10

 

Added to collection

 29th Nov 2008

Herd No: EL83303024

 

Animal ID: EL833030240246

[IAH/KC1]

Supplied by Olga Mangana:

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[Stars No.  A169/08 no 2]

 

 

2006

CYP2006/02

BTV-16

Serotype by  Seg-2 RT-PCR

 

Athalassa

Cyprus

(2006)

 

Bovine blood

(isolated in  Greece in eggs on 13th Nov 2006)

 

Shelf D5

box D5β,

rows G1- H10

 

Added to collection on 9th Feb 2007

No 50

(Animal id CY100 000 806)

E1

[IAH/BHK2]

Nomikou Kiki,

 

 Centre of Athens Vet. Institutions, Virus Lab. Athens

 

 

CYP2006/01

BTV-16

Serotype by  Seg-2 RT-PCR

Athalassa

Cyprus

(2006)

Bovine blood

(isolated in  Greece in eggs on 13th Nov 2006)

Shelf D5

box D5β,

rows E1- F10

 

Added to collection on 9th Feb 2007

No 4

(Animal id: CY100 751 618)

E1

[IAH/BHK2]

Nomikou Kiki,

 

 Centre of Athens Vet. Institutions, Virus Lab. Athens

 

Vial position F1G given to C. Batten (IAH Pirbright) 21 Sept 2008

for rimg trial

spare vial  held in misc box

2004

CYP2004/01

BTV

Type 16

 

( identified as BTV by ELISA , SNT& Direct Immunofluorecent test )

 

(serotyped by SN, and confirmed by Seg-2 RT-PCR)

Cyprus,  Ammochostos Achna

 

Animal code: 953/18

(12-02-2004)

From sheep

 ( blood )

( identified by VNT on 10-3-2004 At: Centre of Athens Vet. Institutions, Virus Lab,

by: Dr.Nomikou Kiki & O. Mangana)

Shelf A3,

box A3β,

rows  A1-B10

 

(added to collection on 07 Apr 2004)

(CY953/18/04)

from Blood

 

Vero &

 BHK 21 cells 

 

(VERO1/BHK2)

[IAH/BHK2]

Nomikou Kiki,

 

 Centre of Athens Vet. Institutions , Virus Lab. Athens

1/10a, 1/10b (pDam), Sent to C. Hamblin IAH Pirbright, 12 Oct 2004.

*

 1/10a, 1/10b (G), Sent to S. Rao, IAH Pirbright for cDNA synthesis , to send to P.Roy for VLP production (March 2005)

*

Vial position A3 (G) from freezer A and B given to K. Nomikou (IAH-VBDP)

16th Dec 2009

 

[Seg3: DQ186798 ]

FRA2004/01

BTV-16

Corsica

2004

Isolated  at Maisons Alfort, in 2004

Shelf E2,

box E2β,

rows I1– J10

 

Added to collection on

13th Sept 2007

04.99 W1B1

<<wild>>

E1/BHK1

[IAH/ BHK2]

Supplied by Stephan Zientara and Emmanuel Breard,

AFSSA-Maisons Alfort-France

 

 

IND2004/06

BTV-16

India

 

Shelf D4,

box D4 γ,

rows A1-B10

 

(added to collection on  22nd Nov 2006)

[+IAH/ BHK3]  

Sample given to Sushila maan IAH Pirbright Sept 2006 for sequencing studies

 

IND2004/05

BTV

Type 16

 

(virus serotype identified by RT-PCR assays) 

India

 

Shelf D4

box D4 γ,

rows G1-H10

 

(added  to collection

 24th Nov 2006)

 [+IAH/ BHK3]

 

Sample given to Sushila Maan,

IAH Pirbright Sept 2006 for sequencing studies

 
ITL2004/01

BTV

Type 16

Farm Code 011CT084

Province: Caltagirone (Catania)

April 2004

(April 2004

IZS Palermo, Sicily by

  (Sicily, 19155-156/2004) BHK3

Dr. Annalisa Guercio & Giuseppina Alimena IZS Palermo

 

From Sheep spleen  after vaccination

   

SAD2004/24

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

 

(Isolated at IAH Pirbright by Sue Graham)

 

48577

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 76)

   

SAD2004/23

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

21st July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

 

47600

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 74)

   

SAD2004/22

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

23rd July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

 

48548

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 68)

   

SAD2004/21

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

23rd July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

 

48548

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 65)

   

SAD2004/20

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

23rd July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

 

48220

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 63)

   

SAD2004/19

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

23rd July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

 

48220

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 62)

   

SAD2004/18

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

 

(Isolated at IAH Pirbright by Sue Graham)

shelf C2, 
box C2α,
rows E1-F10
 
Added to 
collection on
18th Oct 2005

46942

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 55)

   

SAD2004/17

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

23rd July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

 

48211

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 51)

   

SAD2004/16

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

16th July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

 

47092

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 50)

   

SAD2004/15

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

20th July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

 

47604

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 44 )

   

SAD2004/14

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

30th July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

 

50026

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 38 )

   

SAD2004/13

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

30th July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

shelf C2, 
box C2α,
rows C1-D10
 
Added to 
collection on
18th Oct 2005

50026

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 37)

   

SAD2004/12

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

30th July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

shelf C2, 
box C2α,
rowsA1-B10
 
Added to 
collection on
18th Oct 2005

50026

from frozen blood

 

[IAH/BHK2]

 

[R5/05]

(isolate 36)

   
SAD2004/11

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

28th July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

shelf C1, 
box C1γ
rows I1-J10
 

Added to collection on

27th Sept 2005

49349

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate34)

   
SAD2004/10

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

29th July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

shelf C1, 
box C1γ
rows G1-H10
 

Added to collection on

27th Sept 2005

49839

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 32)

   
SAD2004/09

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

29th July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

shelf C1, 
box C1γ
rows E1-F10
 

Added to collection on

27th Sept 2005

49839

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 29)

   
SAD2004/08

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

22nd July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

shelf C1, 
box C1γ
rows C1-D10
 

Added to collection on

27th Sept 2005

48537

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 26)

Isolated directly into BHK cells  
SAD2004/07

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

22nd July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

shelf C1, 
box C1γ
rows A1-B10
 

Added to collection on

27th Sept 2005

48537

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 25 )

Isolated directly into BHK cells

 

 
SAD2004/06

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

22nd July 2005

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

Shelf C1, 
box C1β,
rows I1-J10
 
Added to
collection
22nd Sept 2005
48537

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 24)

Isolated directly into BHK cells  
SAD2004/05

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

23rd July 2004

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

  48217

from frozen blood

 

[IAH/BHK1]

[R5/05]

(isolate 16)

Isolated directly into BHK cells  
SAD2004/04

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

27th July 2004

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

Shelf C1, 
box C1β,
rows G1-H10
 
Added to
collection
22nd Sept 2005
47705

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 15)

Isolated directly into BHK cells

 

samples (p) 1/10a + b were given to Adam Meyer

(IAH Pirbright) on 11th Oct 2005

[ S2: M773700

S5: AM773693]

SAD2004/03

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

28th July 2004

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)  

Shelf C1, 
box C1β,
rows E1-F10
 
Added to
collection
22nd Sept 2005

49346

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 12)

Isolated directly into BHK cells

 

samples (p) 1/10a + b were given to Adam Meyer

(IAH Pirbright) on 11th Oct 2005

 [Seg2:AM773699

seg5:AM773692]

SAD2004/02

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

28th July 2004

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)  

Shelf C1, 
box C1β,
rows C1-D10
 
Added to
collection
22nd Sept 2005

49346

from frozen blood

 

[IAH/BHK2]

[R5/05]

(isolate 11)

Isolated directly into BHK cells

 

SAD2004/01

BTV

Type 16

 

(virus species & serotype confirmed by RT-PCR assays)

Sardinia,

Italy

 

28th July 2004

Sheep

 

(Isolated at IAH Pirbright by Sue Graham)

Shelf C1, 
box C1β,
rows A1-B10
 
Added to
collection
22nd Sept 2005

49346

from frozen blood

 

[IAH/E1/BHK3]

[R5/05]

 (isolate 10)

 

 

2003

ZIM2003/10

 

 

BTV Type 16

 

Typed by SNT

Zimbabwe

(Lone Oak)

2003

Culicoides imicola

Shelf B6, 
Box B6α
Rows I1-J10
 
(23 Aug 2005)

 

(E1/BHK1)

[IAH/BHK2]

  [R06/03,No.171]

 

» contents 

 

[Seg3:DQ186828]

ISR2003/01

BTV

Type 16

Israel

Gan Ha-Shomron

Sheep

(11/09/2003

at the Kimron Institute)

Shelf A5,

Box A5β,

Rows A1-B10

(405031

Lab No: 2774)

E1

[IAH-E1/BHK2]

Dr. Hagai Yadin,

Kimron Institute

Israel

*MTA* , not 
to be supplied 
 to 3rd parties 
without written 
consent from 
original source

OIE  report 21st Nov/2003 Vol 16 No. 47

2001

ISR2001/18

 

 

 

 

BTV

Type 16

 

typed by

 Seg-2 RT-PCR and sequence comparisons

Israel,

Yokneam

Sheep (27/Feb/2001 at the Kimron Institute)

Shelf B6, 
box B6γ,
rows A1-B10
 
Added to 
collection 
7th Sept 2005

(300180

Lab No:823/1)

E1

[IAH-BHK4]

 Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not 
to be supplied 
 to 3rd parties 
without written 
consent from 
original source

[Seg2:AM773710

Seg3:DQ186798

Seg5:AM773704]

ISR2001/16

 

 

 

 

BTV

Type 16

 

yped by

 Seg-2 RT-PCR and sequence comparisons

Israel,

Yokneam

Sheep (27/Feb/2001 at the Kimron Institute)

Shelf B6, 
Box B6β
Rows C1-D10
 
(01 Oct 2005)

(300180

Lab No:823/5)

E1

[IAH/BHK4]

 Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not 
to be supplied 
 to 3rd parties 
without written 
consent from 
original source

ISR2001/15

 

 

 

 

 BTV

Type 16

 

yped by

 Seg-2 RT-PCR and sequence comparisons

Israel, Yokneam

Sheep (27/Feb/2001 at the Kimron Institute)

Shelf B6, 
Box B6β
Rows A1-B10
 
(01 Oct 2005)

(300180

Lab No:823/8)

 

E1

[IAH/BHK4]

 Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not 
to be supplied 
 to 3rd parties 
without written 
consent from 
original source

» contents

ISR2001/14

 

 

 

 

 BTV

Type 16

 

yped by

 Seg-2 RT-PCR and sequence comparisons

Israel, Yokneam

Sheep (27/Feb/2001 at the Kimron Institute)

 Shelf B6, 
Box B6α
Rows C1-D10
 
(23 Aug 2005)

(300180

Lab No:823/6)

E1

[IAH/BHK4]

 Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not 
to be supplied 
 to 3rd parties 
without written 
consent from 
original source

 

2000

TUR2000/10

BTV

Type 16

 

 ( Identified as BTV by ELISA: Typed by Seg-2 RT-PCR & sequencing)

Turkey From IZMIR-Dikili; (year 2000)

 

Shelf C3, 
box C3β,

rows I1 - J10

 

(added to collection on 18th Nov 2005)

 (BTTR 2000/10)

E2/BHK4

[IAH/BHK2]

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey 06/10/00 [R13/00 no 10]

 

» contents

 

 [S2: AJ585148

S5: AM773696

S6:AJ586693

S10:     ]

TUR2000/09

BTV

Type 16

 

 ( Identified as BTV by ELISA: Typed by Seg-2 RT-PCR )

Turkey from IZMIR-Urla; (year 2000)

 

 Shelf A4,

Box A4α,

Positions

 A1-B10

 (BTTR 2000/9)

(E1/BHK5)

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey 06/10/00 [R13/00 no 9]

 

» contents

 

 

[Seg10:  ]

TUR2000/08

BTV

Type 16

 

 ( Identified as BTV by ELISA: Typed by Seg-2 RT-PCR )

Turkey From MANISA-ALASEHIR (year 2000)

 

 

Shelf A4,

Box A4α

Positions

C1-B10

 (BTTR 2000/8)

(E3/BHK6)

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey 06/10/00 [R13/00 no 8]

 

[S10:     ]

TUR2000/02 

BTV

Type 16

 

 ( Identified as

 BTV by ELISA:

 

Typed by SNT

& Seg-2

RT-PCR + sequencing)

From MANISA- Salihli

(year 2000)

 
Shelf C3, 
box C3α,
rows G1 - H10
 
(added to 
collection on 
15th Nov 2006)
 

  (BTTR2000/2)

(E2/BHK7)

 

[IAH/BHK2]

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey

 

 06/10/00

[R13/00 no 2]

 

 [S2: S6:AJ586692

S10:   ]

TUR2000/01

 

BTV

Type 16

 

 ( Identified as BTV by ELISA: Typed by SNT & Seg-2 RT-PCR & sequencing)

IZMIR- Seferihisar

(year 2000)

 

Shelf B2, boxB2α,

rows G1-H10

 

(added to collection on 16th Mar 2005)

 

 (BTTR 2000/1)

(E3/BHK7)

[IAH- BHK2]

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey 06/10/00 [R13/00 no 1]

 

» contents

 

 

 

[Seg2:

Seg3: DQ186827  Seg6:AJ586691

Seg10:   ]

1999

GRE1999/28

BTV Type 16

 

(identified as BTV by ELISA and Seg-7 RT-PCR :

 Typed by serum neutralisation assay)

Greece,

 Rodopi

Kerasia-Sapes

(North)

 

41 10’N, 25 16’E

 

(sample taken 13th Aug 1999)

Sheep (blood)

 

At: Centre of Athens Vet institutions, virus lab

 

 isolated by K. Nomikou and

O. Mangana

Shelf A12,

box A12 γ

rows F1-G10

 

(Added to collection on 18th July 2008)

Animal code 1

E1/BHK5

[+IAH/BHK1]

Nomikou Kiki

 

 Centre of Athens Vet. Institutions, Virus Lab, Athens

 

 

GRE1999/13

BTV

Type 16

 

 ( Identified as BTV by ELISA

 & RT-PCR: Typed by SNT )

Damatria, Rhodes,

Greece (SE)

 

[36º 23΄Ν,

28º 06΄ Ε]

 

 (19 Aug 1999)

From sheep

 (blood)

at : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana

Shelf B3,

box B3α

rows I1-J10

 

 

(added to collection on 30th Sept 2004)

GR308/99RS

BHK 21 CELLS

 

blood

 

(E2BHK9)

+

[IAH/BHK2]

Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.

 

Seg 10 sequenced at E2/BHK12

[Seg2:AM773709  Seg3:DQ186821

Seg5:AM773703

Seg10:AY449652]

1991
ISA1991/01

BTV

Type 16

Indonesia (01/08/91)

NK

Shelf B1,

box B1α

rows A1-B10

 (1169)

E1/ASL1/BHK5

 

[IAH/BHK1]

 

Duplicate samples

A1(g) supplied on 19th Dec 2007, to JongChul, Jeong

Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

 [Seg2:

Seg3:DQ186812  Seg6:AJ586690]

1986
AUS1986/02

BTV

Type 16

Australia, Northern Territories 

1986

(G. Gard)

Shelf A6,

boxA6β,

rows A1-B10

(DPP0965)

(V965)

(8609-15-1100)

BHK2/V1

[IAH-BHK1]

Dr. Ian Pritchard

AAHL

Geelong,

Australia

*MTA* , not 
to be supplied 
 to 3rd parties 
without written 
consent from 
original source

[S3:DQ186791]

1982

NIG1982/10

BTV

Type 16

Nigeria (22/08/82)

NK

Shelf A6,

box A6β,

rows I1-J10

 

  (N-434)

E1/BHK4

[IAH-BHK1]

 

aliquots 1/10a, 1/10b pDam, Sent to Kiki Nomikou, Athens Vet Labs ,  Greece, Oct 2004

[Seg2:

Seg3: DQ186819  Seg6:AJ586694]

Unknown isolation date

TUR----/03

BTV 16

Identified by

Seg-2 specific

RT-PCR

Turkey

Isolated in Turkey

Shelf A17,

box A17 β,

rows E1 –F10

 

[IAH BHK1]

Supplied by Mahmod Azimi

 

Razi Vaccine & Serum Research Institute, Karaj, Iran P.O.box:

31975/148

 

[Stars No.  A140/08 no 64]

Although the sample came via Iran, it is believed that it originated from Turkey

 

TUR----/02

BTV-16

Turkey

(isolated in Turkey)

 

A140/08 number 64

KC cells

 

[IAH/BHK1]

supplied by Dr. Mahmod Azimi

 

Razi Vaccine & Serum Research Institute, Karaj, Iran P.O.box:

31975/148

 

 

*IAH dsRNA virus collection number: [Country code, year / sample number] rrrr = reference strain vvvv = vaccine strain]

 

 

References

 

1:  Prasad G, Garg AK, Minakshi , Kakker NK, Srivastava RN.(1994) Isolation of bluetongue virus from sheep in Rajasthan State, India.  

Rev Sci Tech. 13, 935-938


2:  Mertens PP, Maan NS, Prasad G, Samuel AR, Shaw AE, Potgieter AC, Anthony SJ, Maan S. (2007 ) Design of primers and use of

RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains. J Gen Virol. 88, 2811-2823.

 

3:  Shaw AE, Monaghan P, Alpar HO, Anthony S, Darpel KE, Batten CA, Guercio A, Alimena G, Vitale M, Bankowska K, Carpenter 
S, Jones H, Oura CA, King DP, Elliott H, Mellor PS, Mertens PP. (2007 Development and initial evaluation of a real-time RT-PCR assay 
to detect bluetongue virus genome segment 1.) J Virol Methods.145, 115-126.  
 

4. Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC (2007) Analysis and phylogenetic comparisons of full-length VP2 genes

 of the 24 bluetongue virus serotypes. J Gen Virol. 88, 621-630

 

5. BLUETONGUE IN ISRAEL Additional information  OIE  report 21st Nov/2003 Vol 16 No. 47

 

 

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(ReoID :  Edited by Peter. P. C. Mertens and  Houssam Attoui)