dsRNA viruses: Isolates of Bluetongue virus type 24 (BTV-24) in the dsRNA virus collection at IAH Pirbright

Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus, serotype 24 (BTV-24)

*IAH dsRNA virus collection number

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

(identification method)

Place of original sample eg country town
[grid reference]

(date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

(Date added to collection)

Suppliers
Isolate Designation

Original sample material,

Adapted to which cell types

(Passage history of sample)

[IAH passage history]

* Original sample supplied to the collection by person / institution

[IAH, ISIS sample number]

Comments

References & accession numbers

RSArrrr/24

BTV

Type 24

Supplied by OVI
S. Africa*(02/10/87)

NK

Shelf C3,

box C3α,

rows A1 - B10

(added to

collection on

15th Nov 2006)

(Ref. Strain)

Adapted to BHK cells

(E1/CER4/BHK2)

[IAH/BHK4]

*

Duplicate samples A1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do,

440-330, Korea
*
Vial B1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco 29th June 2009
*

Vial B2 sent to Denis Kolbasov, Russian National Institute of Veterinary Virology 29th Sept 2009

*
^{» contents

[S2:AJ585145,

S6:AJ586730]}
Maan et al 2007

2009

ISR2009/19
(ISR2010/03)
BTV-24

Identified as BTV 24 by Seg-2 specific

Real time RT-PCR

Israel

Havat Ronit/ Rishpon

Ovine blood
Isolated from sheep blood in embryonated chicken eggs at Kimron
Shelf B17

box β,

rows E1-F10

^{^{^{Added to collection on

8th Apr 2010}}} 3283/09/1
ECE1
[IAH/V1]
Jacob Brenner , Kimron Institute Israel

Samples arrived 23^rd March 2010

IAH Stars

number
A24/10 18 Clinical signs: Classical ovine BT symptoms

Outbreak data

ISR2009/18

(ISR2010/04)

BTV-24

Identified as BTV 24 by Seg-2 specific

Real time RT-PCR

Israel

Havat Ronit/ Rishpon

From sheep blood

collected

11th Nov 2009

Isolated
From Sheep blood
in KC cells
at IAH

Shelf B17

box β,

rows G1-H10

Added to collection on

8th Apr 2010

3283/09/2

[IAH/KC1]

Jacob Brenner , Kimron Institute Israel

Samples arrived 23^rd March 2010

IAH Stars

number

A24/10 13

Clinical signs: Classical ovine BT symptoms

Outbreak data

ISR2009/16
^{^{^{BTV24

Identified as BTV 24 by Seg-2 specific

Real time RT-PCR}}} ^{^{^{Israel,

from Hadarom, region Eshell,

Sheep sample

1st Oct
2009}}}
^{^{^{From Sheep blood

Isolated in

KC cells

at IAH-P}}}
^{^{^{Shelf B13

box
B13 β,
rows E1-F10

Added to collection on 25th Nov
2009}}}
3042/09

[IAH/KC1]
^{^{^{Dr. Hagai Yadin, Kimron Institute
Israel

Samples arrived 2nd November 2009

[IAH Stars No.

A88/09 01]}}}
outbreak data

ISR2009/12

BTV-24

Identified as BTV by Seg-2 specific

Real time RT-PCR
^{^{^{Israel,

from Almog
Sampled

21^{^{^{^st}}} M^{^{^ay
2009}}}}}
bovine blood

Isolated in cells
at IAH-P
Shelf B10

Box B10 α
Rows Added G1-H10
^{^{^{^{^{^{Added to collection on}}}13th July 2009}}}
Cow number 631
KC1
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15^TH June 2009

IAH Stars

number
A55/09 7 (blood)

ISR2009/11

BTV-24

Identified as BTV by Seg-2 specific

Real time RT-PCR

Israel,

from Kalya

sampled

20^th May 2009

bovine blood

Isolated in eggs at Kimron (Israel)
^{^{^{^{^{^{Added to collection on}}}13th July 2009}}}
Shelf B10

Box B10 α,
Rows Added E1-F10

Cow number 1251

E1
[BHK1 at IAH-P]
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15^TH June 2009

IAH Stars

number
A55/09 6 (eggs)

ISR2009/10

BTV-24

Identified as BTV by Seg-2 specific

Real time RT-PCR

Israel, from Kalya

sampled

20^th May 2009

bovine blood

Isolated in eggs at Kimron (Israel)

Shelf B10

Box B10 α,
Rows Added C1-D10 ^{^{^{^{^{^{Added to collection on}}}13th July 2009}}}
Cow number 1349

E1
[BHK1 at IAH-P]
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15^TH June 2009

IAH Stars

number
A55/09 5 (eggs)

ISR2009/09

BTV-24

Identified as BTV by Seg-2 specific

Real time RT-PCR

Israel,

from Kalya

Sampled

20^th May 2009

cattle blood

Isolated in cells
at IAH-P
^{^{^{^{^{^{Added to collection on}}}13th July 2009}}}
Shelf B10

Box B10 α,
Rows A1-B10
^{^{^{^{^{^{Added to collection on}}}13th July 2009}}}
Cow number 1349
KC1
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15^TH June 2009

IAH Stars

number
A55/09 5 (blood)

ISR2009/08

BTV-24

Identified as BTV by Seg-2 specific

Real time RT-PCR

Israel,

from Kalya

Sampled

20^th May 2009

cattle blood

Isolated in eggs at Kimron (Israel)
Shelf B9

Box B9 γ,
Rows Added I1-J10
^{^{^{^{^{^{Added to collection on}}}13th July 2009}}}
Cow number 1255

E1

[BHK1 at IAH-P]

Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15^TH June 2009

IAH Stars

number

A55/09 4 (eggs)

ISR2009/07

BTV-24

Identified as BTV by Seg-2 specific

Real time RT-PCR

Israel,

from Kalya

Sampled

20^th May 2009

cattle blood

Isolated in KC cells

at IAH-P

Shelf B9

Box B9 γ,
Rows Added G1-H10
^{^{^{^{^{^{Added to collection on}}}13th July 2009}}}
Cow number 1255
KC1
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15^TH June 2009

IAH Stars

number
A55/09 4 (blood)

ISR2009/06

BTV-24

Identified as BTV by Seg-2 specific

Real time RT-PCR

Israel,

from Kalya

Sampled

20^th May 2009

cattle blood

Isolated in KC cells

at IAH-P

Shelf B9

Box B9 γ,
Rows E1-F10
^{^{^{^{^{^{Added to collection on}}}13th July 2009}}}
Cow number 1215
KC1
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15^TH June 2009

IAH Stars

number

A55/09 3 (blood)

ISR2009/05

BTV-24

Identified as BTV by Seg-2 specific

Real time RT-PCR

Israel,

from Kalya

Sampled
20^th May 2009
cattle blood

Isolated in eggs at Kimron , Israel
^{^{^{Shelf B9
Box B9 γ,

Rows C1-D10

^{^{^{^{^{^{Added to collection on}}}13th July 2009}}}}}}
Cow number 1392

E1
[BHK1 at IAH-P]
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15^TH June 2009

IAH Stars

number
A55/09 2 (egg)

ISR2009/04

BTV-24

Identified as BTV by Seg-2 specific

Real time RT-PCR

Israel,

from Kalya

Sampled
20^th May 2009

cattle blood

Isolated in KC cells at IAH-P

Shelf B9

Box B9 γ,
Rows A1-B10

^{^{^{Added to collection on}}}13th July 2009

Cow number 1392
KC1
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15^TH June 2009

IAH Stars

number
A55/09 2 (blood)

MAR2009/01 BTV-24 Martinique (France)

(Sample taken Jan 2009) from non-vaccinated cow
Shelf B18,

box B18 γ ,

rows I1-J10

(Added to collection

9^th Aug 2010)

tube labelled:
E1B2 2893
E1/BHK2

[IAH/BHK1 ]
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France

23rd July 2010 Distribution restricted by MTA

[Seg-2: ]

2008

ISR2008/12 ^{^{^{BTV-24

Identified
as BTV by Seg-7 specific

Real time
RT-PCR

Identified
as serotype 24 by sequence analysis}}}
Israel,

from a village called: Hatzav

samples
5TH Nov 2008 Isolated on eggs in Israel from ovine spleen.
Shelf B8,

box B8 γ

rows A1-B10

Added to collection on 5th Mar 2009

institute number

592236

E1
[IAH/E1/BHK2]
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

Approx 17th Dec 2008

[IAH Stars

number
A181/08 7]

ISR2008/11

BTV-24

Identified as BTV by Seg-1 specific

Real time RT-PCR [3]

Identified as serotype 24 by sequence analysis

Israel,

from a village called:

Hatzav

samples

taken

20th Nov 2008

From sheep blood

Isolated in embryonated chicken eggs in Israel

Shelf B8

box B8 β,

rows G1-H10

Added to collection on 5th Feb 2009

Kimron institute No.

592836

E1

[IAH/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

Approx 17th Dec 2008

[IAH Stars

No. A181/08 11]

ISR2008/10 ^{^{^{BTV-24

Identified
as BTV by Seg-1 specific

Real time
RT-PCR [3]

Identified
as serotype 24 by sequence analysis}}}
Israel,

from a village called: Hura

samples

20th Nov 2008

From sheep spleen

Isolated in embryonated chicken eggs in Israel

Shelf B8,

box B8 β,

rows E1-F10

Added to collection on 5th Feb 2009

Kimron institute No. 592898

E1

[IAH/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

Approx 17th Dec 2008

[IAH Stars

No. A181/08 10]

ISR2008/09

BTV-24

Identified as BTV by Seg-1 specific

Real time RT-PCR [3]

Identified as serotype 24 by sequence analysis

Israel,

from a village called: Beer Sheva

samples

9th Nov 2008
^{^{^{From sheep
blood}}}
^{^{^{Isolated
in embryonated chicken eggs in Israel}}}

Shelf B8,

box B8 β,

rows C1-D10

Added to collection on 5th Feb 2009

Kimron institute No.

592395

E1

[IAH/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

Approx 17th Dec 2008

[IAH Stars

No. A181/08 9]

ISR2008/08
BTV-24

Identified as BTV by Seg-1 specific

Real time RT-PCR [3]

Identified as serotype 24 by sequence analysis
Israel,

from a village called: Beer Sheva

samples
9th Nov 2008 ^{^{^{From
cattle blood.}}}
^{^{^{Isolated
in embryonated chicken eggs in Israel}}}

Shelf B8,

box B8 β,

rows A1-B10

Added to collection on 30th Jan 2009

^{^{^{Kimron Institute No.}}}

592394

E1
[IAH/BHK2]
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

Approx 17th Dec 2008

[IAH Stars

No. A181/08 8]

ISR2008/07 ^{^{^{^{^{^{BTV-24

Identified
as BTV by Seg-1 specific

Real time
RT-PCR [3]

Identified
as serotype 24 by sequence analysis}}}}}}
Israel,

from a village called: Menuha

samples
1st Dec 2008 From cattle blood.
Isolated in embryonated chicken eggs in Israel

Shelf B8,

box B8 α,

rows I1-J10

^{^{^{^{^{^{Added
to collection on 30th Jan 2009}}}}}}

^{^{^{Kimron Institute No.}}}

593390

E1
[IAH/BHK2]
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

Approx 17th Dec 2008

[IAH Stars

No. A181/08 6]

ISR2008/06
BTV-24

Identified as BTV by Seg-1 specific

Real time RT-PCR [3]

Identified as serotype 24 by sequence analysis
Israel,

from a village called: Menuha

samples
3rd Nov 2008 From sheep blood
Isolated in embryonated chicken eggs in Israel

Shelf B8,

box B8 α,

rows G1-H10

Added to collection on 30th Jan 2009

^{^{^{Kimron Institute No.}}}⁵⁹²¹⁴⁸

E1
[IAH/BHK2]
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

Approx 17th Dec 2008

[IAH Stars No.

A181/08 5]

ISR2008/05 ^{^{^{BTV-24

Identified
as BTV by Seg-1 specific

Real time
RT-PCR [3]

Identified
as serotype 24 by sequence analysis}}}
Israel,

from a village called: Elishma

samples
24th Nov 2008 From sheep blood.
Isolated in embryonated chicken eggs in Israel

Shelf B8,

box B8 α,

rows E1-F10
Added to collection on 30th Jan 2009
Kimron Institute No. 593011

E1
[IAH/BHK2]
Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

Approx 17th Dec 2008

[IAH Stars No.

A181/08 4]

2007

USA2007/01
BTV- 24

Virus serotype identified by RT-PCR using type specific primers and sequencing (Seg-2) [1].

USA: Marion County Florida

Sample taken 25th Sept 2007

White tailed deer (farmed)

Multiple losses and Clinical disease observed in herd

Isolated in 2007

Shelf A16,

box A16 α,

rows A1-B10

(Added to collection

16^th Oct 2008)

Virus ID: 07-517030

Tube labelled:

517030; IVD BHK1; 10-30-07

(sample prepared
30th Oct 2007)

Isolated from spleen sample

E1/BHK1

virus isolate supplied by / Reagent also available from:

:

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA
nvsluf@aphis.usda.gov

Technical contact in USA:

Donna Johnson

:

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

2005

MAR2005/03

BTV-24

Martinique (France)

(Sample taken Jan 2005)

from non-vaccinated cow

Shelf B18,

box B18 γ ,

rows G1-H10

(Added to collection

9^th Aug 2010)

tube labelled:
BTV-
24/06.01-127
[IAH/BHK1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France

23rd July 2010

Distribution restricted by MTA

[Seg-2 ]

^{^{^{References

1. Maan S, Maan NS, Samuel AR, Rao S,
Attoui H, Mertens PPC (2007) Analysis and phylogenetic comparisons of
full-length VP2 genes

of the 24 bluetongue virus serotypes. J
Gen Virol. 88, 621-630

2. Mertens PP, Maan NS, Prasad
G, Samuel AR, Shaw AE, Potgieter AC, Anthony SJ, Maan S. (2007) Design
of primers and use of RT-PCR
assays for typing European bluetongue

virus isolates: differentiation of field and vaccine strains. J Gen
Virol. 88, 2811-2823.}}}
3: Shaw AE, Monaghan P, Alpar HO, Anthony S, Darpel KE, Batten CA, Guercio A, Alimena G, Vitale M, Bankowska K, Carpenter S, Jones H, Oura CA, King DP, Elliott H, Mellor PS, Mertens PP. (2007 Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1.) J Virol Methods.145, 115-126.