dsRNA viruses: Isolates of Bluetongue virus type 4 (BTV-4) in the dsRNA virus collection at IAH Pirbright

Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus serotype 4 (BTV-4)
IAH dsRNA virus collection number ( ICTVdb isolate Accession Number)*	Virus Species & Serotype (identification method)	Place of original sample eg country town [grid reference] (date original sample was taken)	Isolated from which host species e.g cow, sheep, etc (Date of virus isolation, where, by whom)	Position of storage in reference freezers (Date added to collection)	Suppliers Isolate Designation	Original sample material, Adapted to which cell types (Passage history of sample) [IAH passage history]	* Original sample supplied to the collection by person / institution [IAH, ISIS sample number]	Comments	References & accession numbers
Reference and vaccine strains
RSArrrr/04	BTV Type 4	Supplied by OVI S. Africa (06/05/82)	NK	Shelf B3, box B3β, rows C1-D10 Added to collection on 9th May 2005	(Ref. Strain)	(BHK7) + [IAH/BHK2]		Duplicate samples C1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong,JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea * Duplicate samples, box B3β - position D2(p) supplied to Oscar R. González Llamazares (Avenida Párroco Pablo Díez 49-57, 24010 - Leon - Spain, 22nd Nov 2007) * Vial D2 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco 29th June 2009 * Vial C3 sent to Denis Kolbasov, Russian National Institute of Veterinary Virology 29th Sept 2009 * One vials, positions D3 (P) given to H. Attoui (IAH-P) 20th May 2010: Spare sample relocated to E18 – E18 γ miscellaneous box C position E9 at the bottom of freezer A * Vial E sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011 * » contents [S2: AJ585125, S3: DQ186794, S6:AJ586699] Sellers et al 1979 Maan et al 2007
RSAvvv7/04	BTV Type 4	S.Africa 14/Apr/02	NK	Shelf A1, Box A1α, Positions A1-B10	Vaccine strain [R8/02 (no7)]	BHK [BHK1]	Sue Graham / IAH (June 2002)		[S10: ]
RSAvvv5/04	BTV Type 4	S.Africa 14/Apr/02	NK	shelf A1, box A1β, rows G1-H10	Vaccine strain [R8/02 (no 5)]	BHK [IAH-BHK1]	Sue Graham / IAH (June 2002)		» contents
RSAvvv4/04	BTV Type 4					[IAH/BHK1]	derived from RSAvvv1/04
RSAvvv3/04	BTV Type 4	S.Africa 14/Apr/02	NK	Shelf C4, box C4γ rows A1 - B10 (added to collection 8th Dec 2005)	Vaccine strain [R8/02 (no 3) & R7/02]	BHK cells Previous passage history unknown BHK1 [IAH/BHK1]	Sue Graham / IAH (June 2002)	PAGE shows two distinct segment 10 bands	[S2: AJ585168, S6:AJ586676 S9:EU220292]
RSAvvv1/04	BTV Type 4						Used to generate RSAvvv4/04
RSAvvvv/04	BTV Type 4	OVI S. Africa	NK	Shelf B3, Box B3γ, Rows I -J10 (Added to collection on 07/04/03)	(Vaccine strain)	BHK cells [IAH/BHK1]	Sue Graham / IAH (June 2002)	Duplicate sample (position J10) given to Carrie Batten, IAH Pirbright 18th Jul 2006 Vial position J9 (A) given to Carrie Batten (IAH) 20th July 2009. Vial J9 from Freezer B put in Misc box B in freezer A position B7 » contents [S5:AM778439]
TURvvvv/04	BTV Type 4 (Typed by Serum Neutralisation Test)	Turkey	NK	Shelf A1, box A1β, rows I1-J10 (added to collection 7th Apr 2003)	vaccine strain	[IAH-BHK1]		Tubes 1/10(g)-I1 & 1/10(p)-J1 supplied to S. Anthony & H. Jones, IAH Pirbright 07-06-2004 Vial position J10 (A) given to Carrie Batten (IAH) 20th July 2009. Vial J10 from Freezer B put in Misc box B in freezer A position B6 contents [ S6:AJ586680 ]
2011
CYP2011/05	BTV-4 Identified as BTV-4by Seg-2 specific Real time RT-PCR	Cyprus Poli Chryscchous, region Paphos Sample taken 21^st Oct 2011	From sheep blood Isolated in KC cells at IAH-P	Shelf Box Rows Added to collection on	10432/30 Animal id: CY236607625	[KC1]	Vasiliki Christodoulou Virology lab, nicosia Samples arrived 7^th November 2011 IAH Stars number A72/11 31
CYP2011/04	BTV-4 Identified as BTV-4by Seg-2 specific Real time RT-PCR	Cyprus Poli Chryscchous, region Paphos Sample taken 21^st Oct 2011	From sheep blood Isolated in KC cells at IAH-P	Shelf Box Rows Added to collection on	10432/25 Animal id: CY246485554	[KC1]	Vasiliki Christodoulou Virology lab, nicosia Samples arrived 7^th November 2011 IAH Stars number A72/11 27
CYP2011/03	BTV-4 Identified as BTV-4by Seg-2 specific Real time RT-PCR	Cyprus Poli Chryscchous, region Paphos Sample taken 20^th Oct 2011	From sheep blood Isolated in KC cells at IAH-P	Shelf Box Rows Added to collection on	10418/5 Animal id: CY236595098	[KC1]	Vasiliki Christodoulou Virology lab, nicosia Samples arrived 7^th November 2011 IAH Stars number A72/11 13
CYP2011/02	BTV-4 Identified as BTV-4by Seg-2 specific Real time RT-PCR	Cyprus Poli Chryscchous, region Paphos Sample taken 20^th Oct 2011	From sheep blood Isolated in KC cells at IAH-P	Shelf Box Rows Added to collection on	10418/1 Animal id:CY22181637	[KC1]	Vasiliki Christodoulou Virology lab, nicosia Samples arrived 7^th November 2011 IAH Stars number A72/11 09
CYP2011/01	BTV-4 Identified as BTV-4by Seg-2 specific Real time RT-PCR	Cyprus Poli Chryscchous, region Paphos 20^th Oct 2011	From sheep blood Isolated in KC cells at IAH-P	Shelf Box Rows Added to collection on	10417/10 Animal Number CY222252922	[KC1]	Vasiliki Christodoulou Virology lab, nicosia Samples arrived 7^th November 2011 IAH Stars number A72/11 07
2010
ISR2010/25	* BTV-4 Identified as BTV-4 by Seg-2 specific Real time RT-PCR	Israel	Isolated from cattle blood at IAH	Shelf C14, box C14 α, rows G1-H10 Added to collection 4th July 2011	Calf purchased for KVI 872 KVI Id	[IAH/KC1]	Jacob Brenner, Kimron Institute Israel Samples arrived 6^TH April 2011 IAH Stars number A32/11 7	* Note original blood positive for BTV-4 and 8
ISR2010/14	BTV-4 Identified as BTV-4 by Seg-2 specific Real time RT-PCR	14/10/10 Lachis, Israel	Sheep blood	* Shelf C12, box C12 β, rows E and F Added to collection 21st Jan 2011	2769/2	[IAH/KC1]	Jacob Brenner,Kimron Institute Israel Samples arrived 24^th November 2010 IAH Stars No. A114/10 12	* Note: only 9 of pdam and glycerol in each freezer
ISR2010/09	BTV-4 Identified as BTV-4 by Seg-2 specific Real time RT-PCR	Israel Kfar Glicson, Sample taken 5th Oct 2010	Cattle blood	Shelf C11 Box C11 γ, rows E and F Added to collection on 14th Jan 2011	2679	[IAH/KC1]	Jacob Brenner Kimron Institute Israel Samples arrived 24^th November 2010 IAH Stars number A114/10 06	(note only 9 pdam in freezer B)
ISR2010/05	BTV-4 Identified as BTV 4 by Seg-2 specific Real time RT-PCR	Israel from Bet Kama Sample taken 7th Jan 2010	Cattle blood Isolated in KC cells at IAH-P	Shelf B17, box β, rows I1-J10 Added to collection 8th Apr 2009	1047/10/2	[IAH/KC1]	From: Jacob Brenner , Kimron Institute Israel Samples arrived 23^rd March 2010 IAH Stars number A24/10 15	Clinical signs: emphysema
ISR2010/02	BTV-4 Identified as BTV 4 by Seg-2 specific Real time RT-PCR	Israel, from Dorot Sample taken 8th Jan 2010	Cattle blood Isolated in KC cells at IAH-P	Shelf B17 box β, rows C1-D10 Added to collection 8th Apr 2009	3100/09/1	[IAH/KC1]	Jacob Brenner , Kimron Institute Israel Samples arrived 23^rd March 2010 IAH Stars number A24/10 05	Clinical signs: Emphysema, respiratory distress, abortion
ISR2010/01	BTV-4 Identified as BTV 4 by Seg-2 specific Real time RT-PCR	Israel, from Dvir Sample taken 4th Jan 2010	Cattle blood Isolated in KC cells at IAH-P	Shelf B17, box β, rows A1-B10 Added to collection 8th Apr 2009	1035/10/3	[IAH/KC1]	Jacob Brenner , Kimron Institute Israel Samples arrived 23^rd March 2010 IAH Stars number A24/10 01	Clinical signs: Subcutaneous emphysema, Died
QTR2010/01	BTV-4 Identified as BTV-4 by Seg-2 specific Real time RT-PCR	Qatar		Shelf B18, box α, rows E1-E10 added to collection 25th may 2010				Insufficient material to store more samples . All samples stored with glycerol. Freezer B only contains samples E1-E9
SPA2010/01	BTV-4 Identified as BTV-4 by Seg-2 specific Real time RT-PCR in Spain	Cadiz	Bovine Blood Isolated at LCV Spain	Shelf C12, box C12 β, rows I1-J10 Added to collection on 2nd Feb 2011	5762/10 sample 1	E1/C6/36-1/ BHK3 [IAH/BHK1]	Marta Vigo Martin LCV Algete Spain	(MTA)
2009
ISR2009/17	BTV4 Identified by Seg-2 specific Real time RT-PCR [LSI]	Israel From Hadaron region Tekuma Sheep sample 1^st Oct 2009	Isolated in KC cells at IAH-P	Shelf B13, box B13 beta, rows G1-H10 Added to collection on 25th Nov 2009	3096/09	[IAH/KC1]	Dr. Hagai Yadin, Kimron Institute Israel Samples arrived 2nd November 2009 [IAH Stars No. A88/09 05]	Outbreak data
MOR2009/14	BTV4 Confirmed by real time RT-PCR Seg 2	Sheep sampled in Marrakech Morocco on 29/10/09	Ovine blood Isolated at IAH Pirbright	Shelf Box Rows (added to collection on ??)	410/09-1	[IAH KC2]	Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco A94/09 10	Derived from MOR2009/10 Distribution restricted by MTA
MOR2009/12	BTV4 Confirmed by real time RT-PCR Seg 2	Sheep sampled in Azrou Morocco on 28/10/09	Ovine blood Isolated at IAH Pirbright	Shelf ?? Box ?? Rows ?? (added to collection on 27th Nov 2009 )	407/09-4	[IAH/KC2]	Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco A94/09 07	Derived from MOR2009/07 * 100ml of this passage level sent to Michael Eschbaumer at FLI Rheims Germany , as a vaccine challenge strain under MTA *
MOR2009/10	BTV4 Confirmed by real time RT-PCR Seg 2	Sheep sampled in Marrakech Morocco on 29/10/09	Ovine blood Isolated at IAH Pirbright	Shelf B14 Box B14 α Rows I1-J10 (added to collection on 1st Dec 2009 )	410/09-1	IAH KC1	Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco A94/09 10	(MTA) * Duplicate vials, positions J1 (p) given to K. Nomikou (IAH-P) 15th Apr 2010 * Duplicate vials, positions J2 (P) given to K. Nomikou (IAH-P) 20th May 2010 * Duplicate vials, positions J3 (P) given to K. Nomikou (IAH-P) 13th Dec 2010 * Used to generate MOR2009/13 Outbreak data
MOR2009/09	BTV4 Confirmed by real time RT-PCR Seg 2	Sheep sampled in Azrou Morocco on 28/10/09	Ovine blood Isolated at IAH Pirbright	Shelf B14 Box B14 α Rows G1-H10 (added to collection on 1st Dec 2009)	407/09-7	IAH KC1	Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco A94/09 09	(MTA) Outbreak data Vial position G1 (g) freezer A and B was sent to Javier Ortego Valdeolmos in Madrid on 15th Dec 2010 * Vial position G2 (g A +B) used on 21st Feb 2011 to prepare passage level KC2, then sent to E. Veronesi and G. Venter in OVI, Onderstepoort South Africa for transmission studies
MOR2009/08	BTV4 Confirmed by real time RT-PCR Seg 2	Sheep sampled in Azrou Morocco on 28/10/09	Ovine blood Isolated at IAH Pirbright	Shelf B14 Box B14 α Rows E1-F10 (added to collection on 27th Nov 2009 )	407/09-6	IAH KC1	Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco A94/09 08	(MTA) Outbreak data
MOR2009/07	BTV4 Confirmed by real time RT-PCR Seg 2	Sheep sampled in Azrou Morocco on 28/10/09	Ovine blood Isolated at IAH Pirbright	Shelf B14 Box B14 α Rows C1-D10 (added to collection on 27th Nov 2009 )	407/09-4	IAH KC1	Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco A94/09 07	* Duplicate vial positions C10 (G) sent to Marta Vigo Martin, Algete Spain on 15th Dec 2009 under MTA * Duplicate vials, position C1 given to C. Batten (IAH-P) 28th April 2010 for Ring Trial * Used to generate MOR2009/12 as a vaccine challenge strain Outbreak data
OMN2009/10	BTV-4 Identified as BTV by Seg-7 specific Real time RT-PCR Identified as BTV-4 by Seg-2 Real-time RT-PCR	Sohar in Oman Samples collected 24th May 2009	Fallow deer blood Isolated in KC cells at IAH-P	Shelf B9, box B9 β, rows I1- J10 Added to collection 17th June 2009	Oman designation: fallow deer edta	[IAH/KC1]	Masha Japhet, Oman Samples arrived Approx 15^TH May 2009 IAH Stars No. A46/09 26	not all +ve animals showed clinical signs of disease. Only Wiltshire sheep, Arabian gazelles, fallow deer and blackbucks were affected . Only one multi-horn sheep died, showing similar signs and post mortem changes, but all of the other live animals sampled were -ve. The +ve Cattle, buffalo, goats and reem gazelles showed no signs of disease . Strains of BTV-1, BTV-8 and BTV-16 were also isolated in early 2009 (see: OMN Isolates)
OMN2009/09	BTV-4 Identified as BTV by Seg-7 specific Real time RT-PCR Identified as BTV-4 by Seg-2 Real-time RT-PCR	Sohar in Oman Samples collected 24th May 2009	gazelle blood Isolated in KC cells at IAH-P	Shelf B9, box B9β, rows G1- H10 Added to collection 17th June 2009	Oman designation: reem gazal EDTA	[IAH/KC1]	Masha Japhet, Oman Samples arrived 15^th May 2009 IAH Stars number A46/09 5	not all +ve animals showed clinical signs of disease. Only Wiltshire sheep, Arabian gazelles, fallow deer and blackbucks were affected . Only one multi-horn sheep died, showing similar signs and post mortem changes, but all of the other live animals sampled were -ve. The +ve Cattle, buffalo, goats and reem gazelles showed no signs of disease . Strains of BTV-1, BTV-8 and BTV-16 were also isolated in early 2009 (see: OMN Isolates)
2008
ISR2008/04	BTV-4 Identified as BTV by Seg-1 specific Real time RT-PCR [3] Identified as serotype 4 and 24 by Seg-2 specific real time RT-PCR BTV-24 confirmed by sequence analyses	Israel, from a village called: Enon samples 24th Nov 2008	Spleen (species not recorded) Isolated on eggs in Israel	Shelf B8, box B8 α, rows C1-D10 Added to collection on 28th Jan 2009	Kimron Institute No. 593014	E1 [IAH/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel Samples arrived Approx 17th Dec 2008 [IAH Stars No. A181/08 12]
ISR2008/02	BTV-4 and BTV-24 Identified as BTV by Seg-1 specific Real time RT-PCR [3] Identified as serotype 4 by Seg-2 specific real time RT-PCR	Israel, from a village called: Zde Eliahu samples taken 13th Oct 2008	From cattle blood. Isolated in chicken eggs in Israel	Shelf B7 box B7γ, rows I1-J10 Added to collection on 28th Jan 2009	Kimron Institute No. 591360	E1 [IAH/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel Samples arrived Approx 17th Dec 2008 [IAH Stars No. A181/08 2]
2006
ISR2006/12	BTV-4 Identified as BTV by Seg-7 specific RT-PCR Identified as serotype 4 by Seg-2 specific RT-PCR	Israel, from a village called: Elyakim samples 17th Nov 2006	Isolated in Israel from Sheep showing clinical signs	Shelf D5 box D5β, rows C1-D10 Added to the collection on 2nd Fen 2007	isolate number 532390 (2309)	CE1 [IAH/BHK1]	Dr. Hagai Yadin, Kimron Institute Israel Samples arrived 15th Dec 2006 IAH Stars number R12/06 -1		Outbreak data
ISR2006/10	BTV-4 Identified as BTV by Seg-7 specific RT-PCR Identified as serotype 4 by Seg-2 specific RT-PCR	Israel, from a village called: Alon Hagalil sampled 1st Nov 2006	Isolated in Israel from Sheep showing clinical signs	Shelf D5, box D5β, rows A1-B10 Added 2nd Feb 2007	isolate number 530659 (2231)	CE1 [IAH/BHK1]	Dr. Hagai Yadin, Kimron Institute Israel Samples arrived 15th Dec 2006 IAH Stars number R12/06 -3		Outbreak data Original isolate held in misc box
2005
SPA2005/05	BTV-4 Identified as BTV by RT-PCR and type 2 by type specific RT-PCR	Spain Blood sample taken 10th Aug 2005	Virus isolated from cattle blood sample at IAH Pirbright by Sue Graham	Shelf C3, box C3γ. rows I1-J10 (added to collection 25th Nov 2005)	blood sample number 5372-8	[IAH/E1/BHK2]	Miguel Angel Jiménez-Clavero Departamento de Enfermedades Emergentes [R10/05 - sample 6]	sequence analyses of Seg-2 show that this isolate is closely related to isolates SPA2004/01 & SPA2004/02 OIE report -14th Oct 2005 Vial position F1G given to C. Batten (IAH Pirbright) 21 Sept 2008 for rimg trial spare vial held in misc box [S5: AM778441]
SPA2005/04	BTV-4 Identified as BTV by RT-PCR and type 2 by type specific RT-PCR	Spain Blood sample taken 10th Aug 2005	Virus isolated from cattle blood sample at IAH Pirbright by Sue Graham	Shelf C3, box C3γ. rows G1-H10 (added to collection 25th Nov 2005)	blood sample number 5372-7	[IAH/E1/BHK2]	Miguel Angel Jiménez-Clavero Departamento de Enfermedades Emergentes [R10/05 - sample 5]	sequence analyses of Seg-2 show that this isolate is closely related to isolates SPA2004/01 and SPA2004/02	OIE report - 14th Oct 2005
SPA2005/03	BTV-4 Identified as BTV by RT-PCR and type 2 by type specific RT-PCR	Spain Blood sample taken 10th Aug 2005	Virus isolated from cattle blood sample at IAH Pirbright by Sue Graham	Shelf C3, box C3γ. rows E1-F10 (added to collection 25th Nov 2005)	blood sample number 5372-6	[IAH/E1/BHK2]	Miguel Angel Jiménez-Clavero Departamento de Enfermedades Emergentes [R10/05 - sample 4 ]	sequence analyses of Seg-2 show that this isolate is closely related to isolates SPA2004/01 and SPA2004/02	OIE report - 14th Oct 2005
SPA2005/02	BTV-4 Identified as BTV by RT-PCR and type 2 by type specific RT-PCR	Spain Blood sample taken 10th Aug 2005	Virus isolated from cattle blood sample at IAH Pirbright by Sue Graham	Shelf C3, box C3γ. rows C1-D10 (added to collection 25th Nov 2005)	blood sample number 5372-5	[IAH/E1/BHK2]	Miguel Angel Jiménez-Clavero Departamento de Enfermedades Emergentes [R10/05 -sample 3]	OIE report - 14th Oct 2005 sequence analyses of Seg-2 show that this isolate is closely related to isolates SPA2004/01 and SPA2004/02 * Sample C1 from both freezers given to K. Nomikou (IAH) , for sequencing on 7th July 2011 *
2004
CYP2004/03	BTV Type 4 (Identified as BTV by ELISA & Typed by serum neutralisation and RtPCR)	Ammochostos, Cyprus (12-2-2004)	Sheep (spleen) Isolated on 10-3-2004 at: Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou & O.Mangana	Shelf B3, box B3β, rows E1-F10 30th Sept. 2004	CY1035/1/04	CPAE& BHK 21 Cells Spleen (CPAE1 BHK4) +[IAH/BHK2]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens	It was initially suggested by SNT that this sample might also contain BTV16, but type specific RT-PCR primers only amplified BTV-4 segment 2	[S3:DQ186799]
MOR2004/02	BTV Type 4 Identified as BTV by Seg-7 RT-PCR, Typed by Seg-2 RT-PCR	El Kebir (north of Morocco)	Sheep	Shelf B2, boxB2β, rows C1-D10 (16/Mar/2005)	BTV/KEK	from blood [IAH-E1/BHK4]	Dr Mehdi El Harrak Virology department Biopharma Laboratory BP4569 Rabat Akkari Morocco	Duplicate samples (1 /10) 'D1-(PDAM)' from freezers a and b, were given to Lydia Kgosana (IAH Pirbright) on 13th Jan 2006	OIE reports from 15th October [Seg3:DQ186817]
SPA2004/02	BTV Type 4	Cadiz province at Jimena de la Frontera	Sheep (Isolated by Sue Graham, IAH Pirbright)	shelf B1, box B1β rows C1 - D10 (05/12/2004)		Blood [IAH: E1/BHK1]	Concepcion Gomez-Tejedor Laboratorio Central de Veterinaria ctra. De Algete km8 28110 Algete (Madrid) [R12/04 (no.21)]	OIE report 15th October 2004 * Duplicate samples 1 /10 'C2-(g)' from freezers a and b, were given to S. Maan (IAH-P) on 27th Sept 2006 * Vial positions E1 (p) were given to Eva Veronesi (IAH Pirbright on 13th May 2010 for Ph.D studies). one of these was retained in Misc box C, bottom of freezer A position E6 *
SPA2004/01	BTV Type 4	Cadiz province at Jimena de la Frontera	Sheep (Isolated by Sue Graham, IAH Pirbright)	shelf B1, box B1β rows A1- B10 (05/12/2004)		blood [IAH/E1/BHK1]	Concepcion Gomez-Tejedor Laboratorio Central de Veterinaria ctra. De Algete km8 28110 Algete (Madrid) [R12/04 (no.4)]	Aliquots 1/10a & 1/10b (p) given to Adam Meyer (IAH, Pirbright - 19th April 2005) Aliquots 2/10a & 2/10b (g) given to Sue Graham (IAH, Pirbright - 20th May 2005) Aliquots B3/10a & B3/10b (p) given to Carrie Batten (IAH, Pirbright - 26th January 2006)	OIE report 15th October 2004
2003
COR2003/06	BTV-4	from Corsica 2003	isolated at AFSSA- Maisons Alfort France in 2003		Original tube marked as: 200	BHK	Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material	Vaccination status of animal unknown
COR2003/05	BTV-4	from Corsica 2003	isolated at AFSSA- Maisons Alfort France in 2003		Original tube marked as: 217	BHK	Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material	Vaccination status of animal unknown
COR2003/04	BTV-4	from Corsica 2003	isolated at AFSSA- Maisons Alfort France in 2003		Original tube marked as: bt4 -03-261	BHK	Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material	Vaccination status of animal unknown
COR2003/03	BTV-4	from Corsica 2003	isolated at AFSSA- Maisons Alfort France in 2003		Original tube marked as: 234	BHK	Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material	Vaccination status of animal unknown
COR2003/02	BTV-4	from Corsica 2003	isolated at AFSSA- Maisons Alfort France in 2003		Original tube marked as: 03.124	BHK	Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material	Vaccination status of animal unknown
COR2003/01 (FRA2003/01)	BTV-4	from Corsica 2003	isolated at AFSSA- Maisons Alfort France in 2003	Shelf E2, box E2β, rows C1– D10 added to collection on 13th Sept 2007	03.134 WIB1: wild BTV-4	E1/BHK1 [IAH/BHK2]	Supplied by Stephan Zientara and Emmanuel Breard, AFSSA-Maisons Alfort-France	Vaccination status of animal unknown * Sample C1 from both freezers given to K. Nomikou (IAH) , for sequencing on 7th July 2011 *
SPA2003/04	BTV Type 4 (Typed by Seg-2 RT-PCR & by Serum Neutralisation Test)	Menorca, Spain (17/11/03)	Sheep (25/11/03 isolated from blood by Sue Graham IAH Pirbright)	Shelf A3, Box A3γ, Positions G1-H10	No. 3 [R16/03]	from blood [IAH-E2/BHK2]	Dr. Ana Canals Centro de Investigacion en Sanidad Animal 28130 Valdeolmos ESPANA	Aliquots 1/10a & 1/10b (p) given to Sue Graham (IAH, Pirbright - 20th May 2005) Duplicate samples (1 /10) 'G1-(g)' from freezers a and b, were given to S. Maan (IAH Pirbright) on 27th Sept 2006	OIE report 19th Dec 2003 Vol. 16 No. 51
SPA2003/03	BTV Type 4 (Typed by Seg-2 RT-PCR and sequence analysis & by Serum Neutralisation Test)	Menorca, Spain (17/11/03)	Sheep (25/11/03 isolated from blood by Sue Graham IAH Pirbright)	Shelf A3, Box A3γ, Positions E1-F10 (25/08/2004)	No.36 [R16/03]	from blood [IAH-E1/BHK3]	Dr. Ana Canals Centro de Investigacion en Sanidad Animal 28130 Valdeolmos ESPANA		OIE report 19th Dec 2003 Vol. 16 No. 51
SPA2003/02	BTV Type 4 (Typed by Seg-2 RT-PCR and sequence analysis & by Serum Neutralisation Test)	Menorca, Spain (17/11/03)	Sheep (25/11/03 isolated from blood by Sue Graham IAH Pirbright)	Shelf A3, Box A3γ, Positions C1-D10 (25/08/2004)	No.34 [R16/03]	from blood [IAH-E1/BHK3]	Dr. Ana Canals Centro de Investigacion en Sanidad Animal 28130 Valdeolmos ESPANA	Isolate used by Shujing Rao, IAH Pirbright for cDNA synthesis , to send to P.Roy for VLP production (March 2005) Samples (p) 1/10a and (p)1/10b, given to Adam Meyer (IAH Pirbright) for virus growth and RNA production for Raniero Lorenzetti (June 2005) Duplicate samples (1 /10) 'C1-(g)' from freezers a and b, were given to S. Maan (IAH Pirbright) on 27th Sept 2006	[S6:AJ783909 S10:AJ783911] OIE report 19th Dec 2003 Vol. 16 No. 51
SPA2003/01	BTV Type 4 (Typed by Seg-2 RT-PCR and sequence analysis & by Serum Neutralisation Test)	Menorca, Spain (17/11/03)	Sheep (25/11/03 isolated from blood by Sue Graham IAH Pirbright)	Shelf A3, Box A3γ, Positions A1-B10 (25/08/2004)	No.32 [R16/03]	from blood [IAH-E1/BHK4]	Dr. Ana Canals Centro de Investigacion en Sanidad Animal 28130 Valdeolmos ESPANA	Duplicate samples (1 /10) 'A1-(g)' from freezers a and b, were given to S. Maan (IAH Pirbright) on 27th Sept 2006	[Seg3:DQ186824 Seg6:AJ783908, Seg10:AJ783910] OIE report 19th Dec 2003 Vol. 16 No. 51
2002
ISR2002/11	BTV Type 4	Israel Beit Dagan	Cow (01/12/2002 at the Kimron Institute)	Shelf A5, Box A5α Rows G1-H10	(369750 Lab No: 2898)	E1 [IAH-E1/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	(MTA)
ISR2002/10	BTV Type 4	Israel , Beit Dagan	Cow (06/11/2002 at the Kimron Institute)	Shelf A5, Box A5α, Positions A1-B10	(367003 Lab No: 2775)	(E1) [IAH/E1/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	isolate related to ISR2002/09 (MTA)	>> contents
ISR2002/09	BTV Type 4	Israel, Beit Dagan	Cow (06/11/2002 at the Kimron Institute)	Shelf B4, Box B4γ, Rows I1-J10 Added to collection on 16th June 2005	(367003 Lab No: 2774)	(E1) + [IAH/E1/BHK4]	Dr. Hagai Yadin, Kimron Institute Israel	isolate related to ISR2002/10 (MTA)
ISR2002/08	BTV Type 4	Israel Beit Dagan	Cow (31/10/2002 at the Kimron Institute)		(366280 Lab No: 2731)	(CE/ )	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2002/07	BTV Type 4	Israel , Beit Dagan	Cow (29/10/2002 at the Kimron Institute)	Shelf A5, Box A5β, Rows I1-J10 10/Sept/2004	(366058 Lab No: 2714)	(E1 ) [IAH/E1/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source
ISR2002/06	BTV Type 4	Israel, Beit Dagan	Cow (24/10/2002 at the Kimron Institute)	Shelf B4, box B4β, Rows G1-H10	(365657 Lab No: 2693)	(E1 ) [IAH/E1/BHK4]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2002/05	BTV Type 4	Israel , Alon Ha-Ggalil	Sheep (22/10/2002 at the Kimron Institute)	Shelf A5, Box A5β, Rows G1 - H10 10/Sept/2004	(365373 Lab No: 2684)	(E1) [IAH/E1/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	isolate related to ISR2002/04 *MTA* , not to be supplied to 3rd parties without written consent from original source
ISR2002/04	BTV Type 4	Israel, Alon Ha-Ggalil	Sheep (22/10/2002 at the Kimron Institute)	Shelf B3, box B3β row G1-H10 added to collection on 09/05/2005	(365373 Lab No: 2683)	(E1 ) + [IAH/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	isolate related to ISR2002/05 *MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2002/03	BTV Type 4	Israel, Beit Dagan	Cow (21/10/2002 at the Kimron Institute)		(365226 Lab No: 2671)	(CE/ )	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source
ISR2002/02	BTV Type 4	Israel Kabul	Sheep (15/10/2002 at the Kimron Institute)	Shelf A5, Box A5α, Positions C1-D10	(364459 Lab No: 2647)	(E1 ) [IAH/E1/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2002/01	BTV Type 4	Israel Beit Dagan )	Cow (06/10/2002 at the Kimron Institute)		(363238 Lab No: 2582)	(CE/ )	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source
ARG2002/04	BTV Type 4	Argentina sample taken 21st Feb 2002	Bovines	Shelf C4, box C4β rows A1 - B10 (added to collection 19th Dec 2005)	[R4/02 (no 102)]	adapted to BHK cells E2/V2/ BHK3 [IAH/BHK2]	Irene Lager (June 2002)
ARG2002/03	BTV Type 4	Argentina 21/02/02	Bovines	Shelf C4, box C4γ rows I1 - J10 (added to collection 8th Dec 2005)	[R4/02 (no 99)]	Adapted to BHK cells E2/V2/ BHK4 [IAH/BHK2]	Irene Lager (June 2002)		>> contents
ARG2002/02	BTV Type 4	Argentina 21/02/02	Bovines	Shelf C4, box C4γ rows G1 - H10 (added to collection 8th Dec 2005)	[R4/02 (no 82)]	Adapted to BHK cells E2/V2/ BHK5 [IAH/BHK2]	Irene Lager (June 2002)
ARG2002/01	BTV Type 4	Argentina 21/02/02	Bovines		[R4/02 (No 20)]	BHK [E2/V2/ BHK2]	Irene Lager (June 2002)		>> contents [S2: S6:AJ586682]
2001
ISR2001/13	BTV Type 4	Israel, kfar Salad	sheep (22/11/2001 at the Kimron Institute)	Shelf B4, box B4α Rows C1-D10 (added to collection on 27th May 2005)	(326038 Lab No: 8244) )	(E1 ) [IAH/E1/BHK4]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2001/12	BTV Type 4	Israel, kfar Shmuel	Sheep (24/10/2001 at the Kimron Institute)	Shelf B4, box B4β, Rows I1-J10	(323282 Lab No: 8063)	(E1 ) [IAH/E1/BHK4]	Dr. Hagai Yadin, Kimron Institute Israel	isolate related to ISR2001/11 *MTA* , not to be supplied to 3rd parties without written consent from original source
ISR2001/11	BTV Type 4	Israel, kfar Shmuel	Sheep (24/10/2001 at the Kimron Institute)	Shelf B4, box B4α Rows E1-F10 (added to collection on 27th May 2005)	(323282 Lab No: 8062)	(CE1 ) [IAH/E1/BHK4]	Dr. Hagai Yadin, Kimron Institute Israel	isolate related to ISR2001/12 *MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2001/10	BTV Type 4	Israel, Givat Ishhihu	Sheep (22/10/2001 at the Kimron Institute)	Shelf A5, Box A5γ, RowsA1-B10 10/Sept/2004	(323033 Lab No: 8042)	(E1 ) [IAH/E1/BHK]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source
ISR2001/09	BTV Type 4	Israel, Givat Ishhihu	Sheep (22/10/2001 at the Kimron Institute)	Shelf A4, Box A4γ, Positions G1-H10	(323033 Lab No: 8041)	(E1) [IAH/E1/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2001/08	BTV Type 4	Israel, Givat Ishhihu	Sheep (22/10/2001 at the Kimron Institute)		(323033 Lab No: 8040)	(CE/ )	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source
ISR2001/07	BTV Type 4	Israel, Givat Ishhihu	Sheep (22/10/2001 at the Kimron Institute)	Shelf A4, Box A4γ, Positions E1-F10	(323033 Lab No: 8039)	(E1) [IAH/E1/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2001/06	BTV Type 4	Israel, Nahalal	Sheep (15/10/2001 at the Kimron Institute)		(322222 Lab No: 7967)	(CE/ )	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source
ISR2001/05	BTV Type 4	Israel, Hatzav	Sheep (28/09/2001 at the Kimron Institute)	Shelf A5, Box A5β, RowsE1-F10 10/Sept/2004	(320226 Lab No: 7782)	(E1) [IAH/E1BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2001/04	BTV Type 4	Israel, Hatzav	Sheep (28/09/2001 at the Kimron Institute)		(320226 Lab No: 7781)	(CE/ )	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source
ISR2001/03	BTV Type 4	Israel, kfar Baruth	Sheep (05/11/2001 at the Kimron Institute)	Shelf A5, Box A5α Rows E1-F10	(324382 Lab No: 8128)	E1 [IAH-E1/ BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents
ISR2001/02	BTV Type 4	Israel, kfar Salad	Goat (12/11/2001 at the Kimron Institute)		(325160 Lab No: 8176)	(CE/ )	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source
ISR2001/01	BTV Type 4	Israel, Bat Slomo	Sheep (05/09/2001 at the Kimron Institute)	Shelf A5, Box A5α, Rows I1-J10	(318830 Lab No: 7678)	E1 [IAH-E1/BHK2]	Dr. Hagai Yadin, Kimron Institute Israel	*MTA* , not to be supplied to 3rd parties without written consent from original source	>> contents [Seg3:DQ186813]
2000
GRE2000/08	BTV Type 4 identified as BTV by ELISA and Seg-7 RT-PCR : Typed by serum neutralisation assay	GREECE , Kavala (North Greece) (sample taken 13th April 2000)	Isolated from adult Culicoides obsoletus At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By: K. Nomikou	Shelf A12 Box A12 α, A1-B10 Added to collection 14th July 2008		from C. Obsoletus : adapted to BHK 21 CELLS BHK6 [+IAH/BHK1]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens
GRE2000/07	BTV Type 4 (Identified as BTV by ELISA , Serum Neutralisation Test & RT-PCR. Serotyped by Serum Neutralisation Test)	Greece, Arta (W Central) Animal code:7 (39^o 20΄ Ν, 21^o 11΄Ε) (3/Oct/2000)	From sheep (Isolated on 13-10-2000 At : Centre of Athens Vet. Institutions, Virus Lab. By: Drs. Nomikou K. & Mangana O.)	Shelf A2, Box A2γ, RowsC1-D10 (20/May/2003	GR-1217/00 ArS (7)	BHK 21 cells (from blood) E2/BHK4 [IAH BHK1]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens	Seg-10 was sequenced at E2/BHK6 duplicate samples (D2p) given to T.Jabbar , IAH Pirbright on 25th Jan 2008	[Seg3: DQ186806 Seg10:AY691692]
GRE2000/06	BTV Type 4	Greece (27/02/00)	NK	Shelf A3, Box A3α, Positions A1-B10	( 384/99 )	(E2/BHK6)		Tubes 1/10(g)-A1 & 1/10(p)-B1 supplied to S. Anthony & H. Jones, IAH Pirbright 07-06-2004	[S10: ]
GRE2000/05	BTV Type 4	Greece (24/02/00)	Goat	Shelf A2, Box A2β, Rows I1-J10 (01/Apr/Nucleotide - Bluetongue virus 4 segment 8, complete sequence, g...2004)	(228/99 )	(E1/BHK5)			>> contents
GRE2000/04	BTV Type 4	Greece (24/02/00)	NK	Shelf A2, Box A2γ, Rows E1-F10 (01/Apr/2004)	(347/99 )	(E2/V4/BHK2)			[S10: ]
GRE2000/03	BTV Type 4	Greece (24/02/00)	Bovine	Shelf A3, Box A3α, Positions C1-D10	(429/99 )	(E2 /V4/BHK3)		Tubes 1/10(g)-C1 & 1/10(p)-D1 supplied to S. Anthony & H. Jones, IAH Pirbright 07-06-2004	>> contents
GRE2000/01	BTV Type 4	Greece (24/02/00)	Sheep	shelf B5, box B5γ, rows E1-F10 (added to collection on 16/08/2005)	(180/99 )	(E1/BHK4) [IAH/BHK1]			[S6:AJ586681 S8: AM900372]
1999
GRE1999/29	BTV4 Identified by BTV group-specific Seg-7 RT-PCR and Real time RT-PCR [3] Typed by SNT, conventional RT-PCR and specific real-time PCR	GREECE Lesbos Aghia Paraskevi ( Central island) 39° 25' N 26° 27' E (25th Nov 1999)	Cattle (spleen ) Isolated at: Centre of Athens Vet. Institutions, Virus Lab, Athens by: Nomikou K.)	Shelf B14, box B14β , rows C1-D10 Added to collection 9th Dec 2009	GR 732/99LB	From cattle spleen adapted to BHK 21 cells ( BHK6 ) [IAH BHK1]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens	Animal in the same herd as the GRE1999/31
GRE1999/27	BTV Type 4 (identified as BTV by ELISA and Seg-7 RT-PCR : Typed by serum neutralisation assay)	Greece , Chalkidiki, Develiki Lerissos (North) (sample taken 21^st Sept 1999)	Sheep (blood) Virus isolated at the Centre of Athens Vet institutions, virus lab, by K. Nomikou and O. Mangana	Shelf A11, box A11α , rows I1-J10 Added to collection 10th July 2008	Animal code 6	Blood to BHK 21 CELLS E1 BHK 4 [+IAH/BHK1]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens
GRE1999/26	BTV Type 4 (identified as BTV by ELISA and Seg-7 RT-PCR : Typed by serum neutralisation assay)	GREECE , Magnesia , Keramidi (Mainland Greece 39° 32' 230''N 22° 56' 102'' E) ( sample taken 15th Oct 1999)	Isolated from adult Culicoides obsoletus At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By: Dr. K.Nomikou	Shelf A11, box A11α , rows G1-H10 (Added to collection 10th July 2008)		from C. Obsoletus : adapted to BHK 21 CELLS BHK5 [+IAH/BHK1]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens
GRE1999/25	BTV Type 4	Greece, Evros ( Doriskos - Feres) (41º 15΄Ν, 26º 19 ΄ Ε) (Original sampling date: 10/08/1999)	Goat (blood)	Shelf B4, box B4α Rows G1-H10 (added to collection on January 2003)	4. F4. GR228/99EG	From Blood (56474) E2/BHK4 [IAH/BHK2]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens	Seg-10 sequenced at E2BHK7 Similar sample to GRE1999/07 Insects caught at time of original sample : C.obsoletus Original host was sick but 0% mortality, 0% morbidity	>> contents [Seg10:AY677622]
GRE1999/24	BTV Type 4	Greece, Rodos (36º 17΄Ν, 27º 51΄ Ε) (1999)	Sheep (blood)	Shelf B2, boxB2β, rows A1-B10 (January 2003)	(GR-180/99RS)	(E2/BHK4) [IAH-BHK2]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens	Seg-10 sequenced at E2/BHK7 Similar isolate to GRE1999/01	>> contents [Seg10:AY677623]
GRE1999/23	BTV Type 4	Greece, Lesbos (39º 20΄Ν, 26º 16 ΄ Ε) (1999)	Sheep (blood)	Shelf C1, box C1α, rows A1-B10 Added to collection 14th Sept 2005	(GR-413/99LS)	E2/ BHK5 [IAH-BHK1]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens (supplied October 2004)	Seg-10 sequenced at E2/BHK8 Similar sample to GRE1999/03	[Seg10:AY677626]
GRE1999/22	BTV Type 4	Greece, Magnesia (39º 10΄Ν, 23º 27΄ Ε) (1999)	Sheep (blood)	shelf B5, box B5α rows E1-F10 (received October 2004	(GR-631/99Mas)	(E2/BHK7) [IAH/BHK2]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens	Seg-10 sequenced at E2/BHK11 similar sample to GRE1999/04	>> contents [Seg10:AY449656]
GRE1999/21	BTV Type 4	Greece, Rodopi (41º 14΄Ν, 25º 53΄Ε) (1999)	Sheep (spleen)	shelf B5, box B5α rows C1-D10 (received October 2004	(GR-280/99Ros)	E2/BHK9 [IAH/BHK2]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens	Seg-10 sequenced at E2/BHK13 Similar sample to GRE1999/08	[Seg10:AY691691]
GRE1999/20	BTV Type 4	Greece, Chios (38º 22΄Ν, 26º 08΄ Ε) (1999)	Cattle (blood)	Shelf B4, Box B4γ. Rows A1-B10 (October 2004)	(GR-378/99CB)	E2/BHK7 [IAH/BHK1]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens	Sreg-10 sequenced at E2/BHK9 Similar sample to GRE1999/09	>> contents [Seg10:AY677620]
GRE1999/19	BTV Type 4	Greece, Lesbos (39º 15΄Ν, 26º 16 ΄ Ε) (1999)	Sheep (spleen)	Shelf B6, box B6γ, rows I1-J10 Added to collection 7th Sept 2005	(GR-382/99LS)	[IAH-BHK5]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens Supplied October 2004)	similar to sample GRE1999/02	>> contents
GRE1999/18	BTV Type 4 (identified as BTV by ELISA & RT-PCR, Typed by neutralisation assay)	Aghia Paraskevi, Levos Greece (NE) (39º 15΄Ν, 26º 16΄ Ε) (17 Sept. 1999)	sheep (spleen) At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana	Shelf B1, box, B1γ, rows C1-D10 30th Sept. 2004	GR382/99LS	BHK 21 cells spleen (E2BHK7) [IAH/BHK2]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.	Seg10 sequenced at E2/BHK10	[S10:AY677624]
GRE1999/17	BTV Type 4 (identified as BTV by ELISA and RT-PCR & Typed by neutralisation assay)	N.Pori, Pieria, Greece (N.Central) (39º 57΄Ν, 22º 34 ΄ Ε) 6th Oct. 1999	From sheep ( spleen) At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana	Shelf B1, Box B1β, Rows I1-J10 30th Sept. 2004	GR457/99PS	BHK 21 CELLS spleen E2BHK7 [IAH/BHK2]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.	Seg-10 sequenced at E2/BHK11	[Seg10:AY449655]
GRE1999/16	BTV Type 4 (identified as BTV by ELISA and RT-PCR & Typed by neutralisation assay)	Psachna, Evia, Greece (Central) (38º 34΄Ν, 23º 38 ΄ Ε) 8th Nov.1999	From sheep ( spleen) At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana	Shelf B3, box B3β, rowI1-J10 30th Sept. 2004	GR610/99EvS	BHK 21 cells spleen (E2BHK7) + [IAH/BHK2]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.	Seg10 sequenced at E2/BHK10	[Seg10:AY677627]
GRE1999/15	BTV Type 4 (identified as BTV by ELISA and RT-PCR & Typed by neutralisation assay)	Kerasia-Kechros- Sapes, Rodopi, Greece (N. Central) (41º 10΄Ν, 25º 16΄ Ε) 13th Aug. 1999	sheep (spleen) At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou & O.Mangana	Shelf B2, boxB2α, rows E1-F10 (30th Sept 2004)	GR279/RoS	BHK 21 spleen (E2BHK6) [IAH-BHK8]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.	Seg-10 sequenced at E2/BHK15	[S3:DQ186809 S5: AM778440, S10:AY677621]
GRE1999/14	BTV Type 4 (identified as BTV by ELISA and RT-PCR & Typed by neutralisation assay)	Kapi, Pelopi Lesvos, Greece (NE) (39º 20΄Ν, 26º 16΄ Ε) (27th Sept. 1999)	sheep (blood) At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou & O.Mangana	Shelf B4, Box B4γ. Rows C1-D10 30 Sept. 2004	GR413/99LS	BHK 21 CELLS blood (E2BHK5) + [IAH/BHK2]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens		[S10: ]
GRE1999/11	BTV Type 4 (Identified as BTV by ELISA , Serum Neutralisation Test & RT-PCR. Serotyped by Serum Neutralisation Test)	Greece Evia, Limni-Kirinthos (Central) (38º 34΄Ν, 23º 38΄Ε) (22/Nov/1999)	From sheep (spleen) (3/Mar/2000 At:Centre of Athens Vet. Institutions, Virus Lab. By: Dr. Nomikou K. & O.Mangana)	Shelf A3, Box A3α, Positions E1-F10 (20/May/2003)	(GR692/99EvS)	BHK 21 cells spleen ( E2/BHK6 ) [IAH-BHK2]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens	Seg-10 sequenced at E2/BHK9 Tubes 1/10(g)-E1 & 1/10(p)-F1 supplied to S. Anthony & H. Jones, IAH Pirbright 07-06-2004	>> contents [Seg10:AY449654]
GRE1999/10	BTV Type 4 (Identified as BTV by ELISA , Serum Neutralisation Test & RT-PCR. Serotyped by Serum Neutralisation Test)	Greece, Lesbos Kalloni (NE) (39º 15΄Ν, 26º 13΄Ε) (24 Sept1999)	Sheep (spleen) (01/ Mar/ 2000 At: Centre of Athens Vet Institutions, Virus Lab. By: K. Nomikou & O.Mangana )	Shelf A3, Box A3α, Positions G1-H10 (20/May/2003)	(GR395/99LS)	BHK 21 cells spleen ( E2/BHK1 ) [IAH-BHK1]	Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens	Seg-10 sequenced at E2/BHK3 Tubes 1/10(g)G1 & 1/10(p)-H1 supplied to S. Anthony & H. Jones, IAH Pirbright 07-06-2004	>> contents [Seg3:DQ186804 Seg10:AY449653]
GRE1999/09	BTV Type 4	Greece, Chios (38º 22΄Ν, 26º 08΄Ε) (1999)	Cattle (blood)	Shelf A3, Box A3α , Rows F1-I10 (January 2003)	(GR-378/99CB)		Dr. Charalabos Billinis Aristotle University Thessaloniki	Similar sample to GRE1999/20	>> contents [S3: DQ186803 S10: ]
GRE1999/08	BTV Type 4	Greece, Rodopi (41º 14΄Ν, 25º 53΄Ε) (1999)	Sheep (spleen)	Shelf A5, Box A5γ, positions I1-J10 (January 2003)	(GR-280/99Ros)	E1/BHK10 [IAH-BHK2]	Dr. Charalabos Billinis Aristotle University Thessaloniki	Similar sample to GRE1999/21	[S10: ]
GRE1999/07	BTV Type 4	Greece, Evros (41º 15΄Ν, 26º 19΄ Ε) (1999)	Goat (blood)	Shelf A5, Box A5β, Rows C1-D10 (January 2003)	(GR-228/99EG)	E1/BHK6	Dr. Charalabos Billinis Aristotle University Thessaloniki	Similar sample to GRE1999/25	>> contents [S10: S3: DQ186802 ]
GRE1999/05	BTV Type 4	Greece, Pieria (39º 57΄Ν, 22º 34΄Ε) (1999)	Sheep (spleen)	Shelf A1, Box A1γ, Positions A1-B10 (January 2003)	(GR-457/99PS)	[IAH - BHK2]	Dr. Charalabos Billinis Aristotle University Thessaloniki		[S10: ]
GRE1999/04	BTV Type 4	Greece, Magnesia (39º 10΄Ν, 23º 27΄Ε) (1999)	Sheep (blood)	Shelf A6, Box A6α, positions A1-B10 (January 2003)	(GR-631/99Mas)	[IAH-BHK3]	Dr. Charalabos Billinis Aristotle University Thessaloniki	Tubes 1/10(g)-A1 & 1/10(p)-B1 supplied to S. Anthony & H. Jones, IAH Pirbright 07-06-2004 Similar samples to GRE1999/22	>> contents [S3: DQ186801]
GRE1999/03	BTV Type 4	Greece, Lesbos (39º 20΄Ν, 26º 15΄Ε) (1999)	Sheep (blood)	Shelf A2, Box A2γ, Rows A1-B10 (January 2003)	(GR-413/99LS)	[IAH-BHK7]	Dr. Charalabos Billinis, Aristotle University Thessaloniki	Tubes 1/10(g)-A1 & 1/10(p)-B1 supplied to S. Anthony & H. Jones, IAH Pirbright 07-06-2004 similar sample to GRE1999/23	[S10: ]
GRE1999/02	BTV Type 4	Greece, lesbos (39º 15΄Ν, 26º 16΄Ε) (1999)	Sheep (spleen)	Shelf A1, Box A1γ: Positions C1-D10 (January 2003)	(GR-382/99LS)	[IAH-BHK5]	Dr. Charalabos Billinis, Aristotle University Thessaloniki	Similar to sample GRE1999/19	>> contents [S10: ]
GRE1999/01	BTV Type 4	Greece, Rodos (36º 17΄Ν, 27º 51΄Ε) (1999)	Sheep (blood)	Shelf A3, Box A3β: Positions F1-I10 (January 2003)	(GR-180/99RS)	(E1/BHK5)	Dr. Charalabos Billinis Aristotle University Thessaloniki	Similar to isolate GRE1999/24	>> contents [S10: S3: DQ186800 ]
1983
SUD1983/03	BTV-4	Shambat, Sudan	Isolated from bovine blood (Sentinel herd)	Shelf B11, box B11 β, rows I1-J10 Added to collection 21st Oct 2009	NY73	E2/BHK3	M.E.H. Mohammed	from same sample as SUD1983/01	Note: Sample was originally supplied as untyped EHDV. However, subsequent work has identified this as an isolate of BTV-4
SUD1983/01	BTV Type 4	Sudan (11/10/83)	NK	shelf B1, box B1α rows G1-H10 (02/Feb/2005)	(NY-73)	(E2/BHK1) [IAH: BHK1]	M.E.H. Mohammed	From same sample as SUD1983/03	>> contents [S2:AJ585166, S3:DQ186825, S6:AJ586677] Note: Sample was originally supplied as untyped EHDV. However, subsequent (sequencing ) work identified this as an isolate of BTV-4
1980
TUR1980/01	BTV Type 4	Turkey (30/07/80)	NK		(Aydin virus)	(BHK3)			[S10: ]
1979
GRE1979/01	BTV Type 4 Identified by virus neutralisation test only	Madamados, (NE) Levos, Greece (NE) (39º 17΄Ν, 26º 20΄Ε) (1979)	From sheep (spleen) Isolated at : Centre of Athens Vet. Institutions, Virus Lab, Athens. By: Dr. O.Mangana	Shelf B3, box B3α rows E1-F10 Added to collection 30 Sept 2004	GR79LS	BHK 21 cells from sheep spleen (E2/BHK6) + [IAH/BHK3]	Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens	Seg-10 sequenced at E2/BHK10	[Seg3:DQ186823 Seg10:AY449650]
1978
TUR1978/01	BTV Type 4	Turkey (00/08/78)	NK			(E1/BHK5)			>> contents [S2: AJ585165, S6:AJ586679 S8: AM900373]
1971
RSA1971/01	BTV-4 confirmed by serology	Grown at IAH in Dorset Poll sheep from a sample of virus originally isolated between 1968-1972			(3050)	no passage in cell culture	This virus has been passed 4 times in sheep It has been grown in Eggs and the last two sheep passes were infected with freeze dried blood plus infected egg material	Freeze dried Virus in sheep blood (Dorset Poll)
1969
CYP1969/01	BTV Type 4	Cyprus (1969)	Cow (Cow tag no A)			(BHK8)			[ S6:AJ586678] Sellers et al 1979