ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of African horse sickness virus (AHSV)

in the dsRNA virus collection at  IAH Pirbright 

 

Return to Virus Collection » Contents

 

IAH dsRNA virus collection number: [Country code,  year / sample number]   rrrr = reference strain     vvvv = vaccine strain] Please send any requests for isolates from this collection to peter.mertens@bbsrc.ac.uk

 

 

 

Reference 
AHSV strains

AHSV-1

AHSV-2

AHSV-3

AHSV-4

AHSV-5

AHSV-6

AHSV-7

AHSV-8

AHSV-9

Untyped

AHSV isolates

 

 

Family: ReoviridaeGenus: Orbivirus,  Species:  African Horsesickness virus (AHSV)

 

Virus Species

 

 

*IAH dsRNA virus collection number:

Serotype

 

(identification method)

Place of original sample

 

eg country town

[grid reference]

 

 

(date original sample was taken)

Isolated from which host species

e.g cow,  sheep, etc

 

(Date of virus isolation, where, by whom )

Position of storage in reference freezers

 

(Date added to collection)

 

 

(Suppliers Isolate Designation)

Adapted to which cell types

 

Original sample material

 

(Passage history of sample)

 

[Passage history at IAH]

 Original sample supplied to the collection by

person / institution

 

[IAH virus

diagnosis, ISIS

sample number]

Comments

CDNA clones,

Purified virus (antigen)

  in collection?

References

&

 accession numbers

 
                   
Reference strains

AHSV-1

RSArrah/01

AHSV-2

RSArrah/02

AHSV-3

RSArrah/03

AHSV-4 AHSV-5 AHSV-6 AHSV-7 AHSV-8 AHSV-9

AHSV - untyped or multiple-types

SENvvv1/MV

AHSV multivalent

Types 1,2,3,4,5,6,7

 

(vaccine)

Senegal

 

 Shelf E3,

box E3α,

rows I1- J10

 

(added to collection

27th Sept 2007)

 

Polyequipeste 
vaccine Contre
la peste equine 
Serotypes de 1a 7 
Flacon de 10doses
Voie sous cutanee
A conserver a
 +4oC/20oC
(usage veterinaire)
Lot no. D-0107
Util.av Sep. 2009
ISRA vaccines

Original bottled vaccine from supplier

 

[IAH/BHK1]

Yaya Thiongane 
Laboratoire National
 de l’elevage 
Et de Recherches
 Veterinaires
Bp 2057
Dakar Hann Senegal
 
(derived from  

SENvvvv/MV)

Virus confirmed as  AHSV

Types 1,2,3,4,5,6,7 by RT-PCR

 

SENvvvv/MV

AHSV multivalent

Types 1,2,3,4,5,6,7

 

(vaccine)

Senegal

 

(added to collection

27th Sept 2007)

 

 

Polyequipeste 
vaccine Contre
la peste equine 
Serotypes de 1a 7 
Flacon de 10doses
Voie sous cutanee
A conserver a
 +4oC/20oC
(usage veterinaire)
Lot no. D-0107
Util.av Sep. 2009
ISRA vaccines

Original bottled vaccine from supplier

Yaya Thiongane 
Laboratoire National
 de l’elevage 
Et de Recherches
 Veterinaires
Bp 2057
Dakar Hann Senegal
 
(used to generate 

SENvvv1/MV

Virus confirmed as  AHSV

Types 1,2,3,4,5,6,7 by RT-PCR

 

1 vial only 

Shelf E18 freezer A 

UAEvvvv/MV

(UAEvvvv/01)

AHS-2, 6, 7 & 8

Polyvalent live  AHSV vaccine produced in Dubai

 

Shelf E5,

box E5α,

rows I1-J10,

 

(Added to collection on 16th Oct 2007)

Polyvalent live  AHSV vaccine - II

[IAH/BHK2]

Dr Ulli Wenery

 
UAEvvvv/01

(UAEvvv2/MV)

AHS 1,3 and 4

Polyvalent vaccine produced in Dubai

Shelf E5,

box  E5γ,

rows A1-B10

 

(Added to collection 

29th Oct 2007)

Polyvalent live  AHSV vaccine - I

[IAH/BHK2]

Dr Ulli Wenery

ETH2010/23

AHSV-2 and 9

 

Identified as AHSV-2 and 9 by Seg-2 specific

 Real time RT-PCR  and sequencing

  Equine Spleen

 Isolated in KC cells at IAH-P

Shelf  C11,

box  C11β,

rows G1-H9

 

Added to collection on 17th Dec 2010
09234/08 [IAH/KC1]

Melesse Balcha

National animal health , diagnostic and investigation centre, Sebeta

Ethiopia

 

Samples arrived

22nd Feb 2010

 

IAH Stars No.
A18/10 02
Used to generate
ETH2010/21
ETH2010/21

AHSV-2 and 9

 

Identified as AHSV-2 and 9 by Seg-2 specific

 real time RT-PCR  and sequencing

  Equine Spleen

 Isolated in KC cells at IAH-P

Shelf  C11,

box  C11 γ, rows A1-B9

 

Added to collection on 17th Dec 2010
09234/08

[IAH/KC1/V1]

Melesse Balcha

National animal health , diagnostic and investigation centre, Sebeta

Ethiopia

 

Samples arrived

22nd Feb 2010

 

IAH Stars No.
A18/10 02
Derived from
ETH2010/23

ZIM2003/11

AHSV- untyped

Zimbabwe

 

Sample collected on 26/03/2003

Horse

(named Young Toddy)

 

plasma

 

BHK4

R4/03

 

Isolate collected by Stuart Gordon

 

 

RSA1960/01 AHSV-8
(plaque cloned)
but also gives positive rRT-PCR for AHSV-5
Onderstepoort South Africa

10th May 1960
    AHSV-8 (G) MB3/BHK7 300692 MB3
[IAH/BHK9]
Supplied by
Rachel O'Hara
IAH
Note:  Plaque cloned x3
 (
O'Hara et al 1998)

strain plaque cloned three times at IAHduring 1991 in BHK cell monolayers
AHSV - 1

RSArah1/01

 

(previously identified as RSArrah/01)

 

AHSV

Type-1

 

 

Shelf B10,

 box B10 β,

row G1-H10

 

Added to collection on 22 Sept 2009

reference strain of AHSV-1

 

(A501)

(MB3)

[IAH/BHK7]

Dr Baltus Erasmus,

 

OVI South Africa(1988)

Howell (1962)

Note: not Plaque cloned

[ S1:AM883164, S2:AM883165,

S3: AM883166, S4: AM883167,

S5: AM883168,  S6: AM883169,

S7: AM883170, S8: AM883171,

S9: AM883172, S10: AM883173 ]

RSArah1/02 Note:  Plaque cloned
mycoplasma contaminated
RSArah1/03 Confirmed as AHSV-1 by Seg-2 specific real-time RT-PCR

No contamination with other serotypes was detected)
Tube identified as AHSV-1 (c) MB3/BHK8 27/01/91 (MB3)
[IAH/BHK10]
From
Rachel O'Hara
IAH
Note:  Plaque cloned x3


strain plaque cloned three times at IAH in BHK cell monolayers during 1991 (O'Hara et al 1998)
RSA1987/02

AHSV

Type-1

        MB3/BHK8
[IAH/BHK5]
   (O'Hara et al 1998)
AHSV - 2

RSArah2/01

(previously identified as RSArrah/02)

 AHSV

Type-2

 

 

Shelf B10,

 box B10 β,

row I1-J10

 

Added to collection on 22 Sept 2009

reference strain of AHSV-2

 

(OD)

(MB3)

[IAH/BHK4]

Dr Baltus Erasmus,

 

OVI South Africa

(1988)

Howell (1962)
RSArah2/02

 AHSV

Type-2

            Note:  Plaque cloned
mycoplasma contamination
RSArah2/03

 AHSV

Type-2

            Note:  Plaque cloned x3
 (
O'Hara et al 1998)

strain plaque cloned three times at IAH in BHK cell monolayers during 1991

ETH2010/22

AHSV-2

 

Identified as AHSV-2 by Seg-2 specific

 Real time RT-PCR  and sequencing

 

Ethiopia

Equine  Spleen

 

 Isolated in KC cells at IAH-P

Shelf  C11,

box  C11β, rows E1-F9

 

Added to collection
 17th Dec 2010   

29654/08

[IAH/KC1]

Melesse Balcha

National animal health , diagnostic and investigation centre, Sebeta

Ethiopia

 

Samples arrived

22nd Feb 2010

 

IAH Stars No.
A18/10 01

Used to generate ETH2010/20

ETH2010/20

AHSV-2

Identified as AHSV-2 by Seg-2 specific

 Real time RT-PCR  and sequencing

Ethiopia Equine  Spleen

Isolated in KC cells at IAH-P

Shelf  C11,

box  C11β, rows  I1-J9

 

Added to collection
 17th Dec 2010   
29654/08 [IAH/KC1/V1]

Melesse Balcha

National animal health, diagnostic and investigation centre, Sebeta, Ethiopia

 

Samples arrived

22nd Feb 2010

 

IAH Stars No.
A18/10 01
Derived from ETH2010/22
ETH2010/02

AHSV-2 

 

Identified as AHSV-2 by Seg-2 specific

 Real time RT-PCR
Ethiopia NVI, ETHIOPIA Vaccine strain

Shelf  C7

Box  C7α

Rows I1-J10

 

Added to collection on  24 Aug 2010
ETH74/10

Vero

 

[IAH/V1]

Esayas Gelaye, National Veterinary Institute

19 Debre Zeit

Ethiopia

 

Samples arrived

22nd July 2010

 

IAH Stars No.

A72/10 74
ETH2010/01

AHSV-2 

 

Identified as AHSV-2 by Seg-2 specific

 Real time RT-PCR 

Ethiopia,

Midie,

 

Sample taken 21 June 2010
Equine Blood

 Isolated in KC cells at IAH

Shelf  C7

box  C7α

rows G1-H10

 

Added to collection on  24 Aug 2010
ETH11/10

[IAH/KC1]

Esayas Gelaye, National Veterinary Institute

19 Debre Zeit

Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No.

A72/10 11
 

GHA2010/05

AHSV-2

identified  by
Seg-2 RT-PCR
and sequencing

Ghana
Polo club

Equine spleen isolated at IAH Pirbright

Shelf B16

Box B16 α

Rows G1-H10

 

(added to collection on 17th Mar 2010)

Suppliers designation 15

[IAH/KC1]

Andy Alhassan

Veterinary services laboratory,

Accra

Ghana

[A12/10 08]

Clinical signs: respiratory symptoms

 

Outbreak reports
GHA2010/04 AHSV-2

identified  by
Seg-2 RT-PCR
and sequencing
Ghana
Polo club

Equine spleen isolated at IAH Pirbright

Shelf B16

Box B16 α

Rows E1-F10

 (added to collection on 17th Mar 2010)

Suppliers designation 05 [IAH/KC1]

Andy Alhassan

Veterinary services laboratory,

Accra

Ghana

[A12/10 05]

Clinical signs: respiratory symptoms

Outbreak reports
GHA2010/03 AHSV-2

identified  by
Seg-2 RT-PCR
and sequencing
Ghana
Polo club

Equine spleen isolated at IAH Pirbright

Shelf B16

Box B16 α

Rows C1-D10

(added to collection on 17th Mar 2010)

Suppliers designation 02 [IAH/KC1]

Andy Alhassan

Veterinary services laboratory,

Accra

Ghana

[A12/10 02] 

Clinical signs: respiratory symptoms

Outbreak reports
GHA2010/02 AHSV-2

identified  by
Seg-2 RT-PCR
and sequencing
Ghana
Polo club

Equine liver isolated at IAH Pirbright

Shelf B16

Box B16 α

Rows A1-B10

 

(added to collection on 17th Mar 2010)

  [IAH/KC1/BHK1]

Andy Alhassan

Veterinary services laboratory,

Accra

Ghana

[A7/10 06]

Clinical signs: respiratory symptoms

Same horse as GHA2010/01

Outbreak reports
GHA2010/01 AHSV-2

identified  by
Seg-2 RT-PCR
and sequencing
Ghana
Polo club

Equine spleen isolated at IAH Pirbright

Shelf B15

Box B15 γ

Rows I1-J10

 

(added to collection on 17th Mar 2010)

  [IAH/KC1/BHK1]

Andy Alhassan

Veterinary services laboratory,

Accra

Ghana

[A7/10 05]


Clinical signs: respiratory symptoms.

Same horse as GHA2010/02

Outbreak reports

SEN2007/05

 

AHSV- 2

Identified as AHSV by serogroup specific RT-PCR

 

Typed by RT-PCR and sequence analysis of Seg-2

Diongo Village, Diourbel, Senegal

 

(Sample

taken on

2nd Sept 2007)

Isolated from horse spleen:

male six year old horse

 

(isolated at IAH Pirbright

Sept 2007)

Shelf E5

Box E5 α

Rows G1-H10

 

 

(added to collection on  16th Oct 2007)

No: 5

[IAH/MB1/BHK2]

A92/07 5

 

 Dr Yaya Thiongane

Chef du Laboratoire de Virologie, Directeur de Recherches
Chef du LNERV
ISRA-LNERV
BP 2057
Dakar-Hann
Sénégal

 

*

Duplicate sample from freezer A & B provided to Kasia Bankowska, IAH  11th July 2008

*
Duplicate vial positions G9 (G) sent to Marta Vigo Martin, Algete Spain on 15th Dec 2009 under MTA
*

Outbreak data

 

SEN2007/04

 

AHSV- 2

Identified as AHSV by serogroup specific RT-PCR

 

Typed by RT-PCR and sequence analysis of Seg-2

Lerane Sambou Village, (Region of Fatick) Senegal

 

(Sample

taken on

24thAug 2007)

Isolated from horse lung:

  Female three year old horse

 

(isolated at IAH Pirbright

Sept 2007)

Shelf E5,

box E5 α,

rows E1-F10

 

(added to collection on  16th Oct 2007)

No: 2

[IAH/MB1/

BHK3]

A92/07 2

 Dr Yaya Thiongane

Chef du Laboratoire de Virologie, Directeur de Recherches
Chef du LNERV
ISRA-LNERV
BP 2057
Dakar-Hann
Sénégal

 

 

 SEN2007/03

 

AHSV- 2

Identified as AHSV by serogroup specific RT-PCR

 

Typed by RT-PCR and sequence analysis of Seg-2

Diourbel, Senegal

 

(Sample

taken on

26thAug 2007)

Isolated from horse spleen:  male six year old horse

 

(isolated at IAH Pirbright

Sept 2007)

Shelf E5

Box E5 β

Rows I1-J10

 

 

(added to collection on  16th Oct 2007)

 

No: 1

[IAH/MB1/

BHK3]

 A92/07 1

Dr Yaya Thiongane

Chef du Laboratoire de Virologie Directeur de Recherches
Chef du LNERV
ISRA-LNERV
BP 2057
Dakar-Hann
Sénégal

 

SEN2007/02

AHSV-2

 

Typed by RT-PCR and sequence analysis of Seg-2

Senegal

2007 

 

From horse

spleen 

 

(isolated at IAH Pirbright

July 2007)

Shelf E1

Box E1 β

Rows I1-J10

(added to collection on  2nd July 2007)

 

note (p)10/10b <1ml position I10 freezer B

 

 

[IAH/MB1/

BHK2] 

Isolate provided by 
Yaya Thiongane 
Laboratoire National
 de l’elevage et
 de Recherches
 Veterinaires
Bp 2057 
Dakar Hann Senegal
-
 
[A59/07 4]
Note:  (p)10/10b <1ml in positions 
I10 & J10 , freezer B
*
1ml sample from position I9g (freezer A) sent to Pfizer / 
Fort Dodge (Joan de Plana) Spain 6th Sept 2010  - 
Corresponding spare vial in freezer B was given to 
E. Veronesi (IAH-P) 26th April 2010
*
Duplicate samples, position E1 A & B 
provided to Kasia Bankowska,  IAH  11th July 2008
 * 

Note: From a horse that died showing typical clinical signs of AHSV.  From the same animal as SEN2007/01 . The animal had been vaccinated 10 days earlier with AHSV-9

*  

Note: this isolate tested negative for BTV (Seg-1) and EHDV (Seg-9)  real-time RT-PCR, and negative for FMDV by 3D assay and 5'UTR assay

*

Outbreak information

SEN2007/01

AHSV-2

Typed by RT-PCR and sequence analysis of Seg-2

Senegal

2007

From horse

blood

 

(isolated at IAH Pirbright July 2007)

Shelf E1
Box E1β
Rows G1-H10

(added to 
collection on 
2nd July 2007)

 

[IAH/MB1/

BHK 2]

Yaya Thiongane 
Laboratoire National
 de l’elevage 
Et de Recherches
 Veterinaires
Bp 2057
Dakar Hann Senegal
 
note (p)10/10b
 <1ml position 
H10 freezer B
 
[ A59/07 1]
From a horse that died showing typical clinical signs of AHSV From the same animal as SEN2007/02
The animal had been vaccinated 10 days earlier with AHSV-9

 Outbreak information
AHSV - 3

RSArrah/03

AHSV

Type-3

 

 

Shelf B10,

 box B10 γ,

row A1-B10

 

Added to collection on 22 Sept 2009

reference strain of AHSV-3

 

(L)

(MB3)

[IAH/BHK3]

Dr Baltus Erasmus,

 

OVI South Africa

(1988)

» Contents

 

Howell (1962)

AHSV - 4

RSArrah/04

AHSV

Type-4

 

 

Shelf B10,

 box B10 γ,

row C1-D10

 

Added to collection on 22 Sept 2009

reference strain of AHSV-4

 

(Vryheid)

(MB3)

[IAH/BHK3]

Dr Baltus Erasmus,

 

OVI South Africa

(1988)

Howell (1962)

ETH2010/08

AHSV-4 

Identified as AHSV-4 by Seg-2 specific real time RT-PCR
Ethiopia NVI, ETHIOPIA Vaccine strain

Shelf  C7,

box  C7 γ,

rows A1-B10

 

Added to collection 

25 Aug 2010
ETH72/10 Vero

[IAH/V1]

Esayas Gelaye, National Veterinary Institute

19 Debre Zeit

Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No. 
A72/10 72
 
ETH2010/07

AHSV-4 

Identified as AHSV-4 by Seg-2 specific real time RT-PCR

Ethiopia

 

Adama

 

29 June 2010
Equine Blood

 Isolated in KC cells at IAH-P

Shelf  C7,

Box  C7β,

rows I1-J10

 

Added to collection 

25 Aug 2010
ETH50/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute

19 Debre Zeit

Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No. 
A72/10 50
 
ETH2010/06

AHSV-4 

Identified as AHSV-4 by Seg-2 specific real time RT-PCR

Ethiopia

 

Debre Zeit

 

Sample taken
24 June 2010
Equine Blood

 Isolated in KC cells at IAH-P

Shelf  C7,

Box  C7β,

rows G1-H10

 

Added to collection 

25 Aug 2010
ETH35/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute

19 Debre Zeit

Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No. 
A72/10 35
 
ETH2010/05

AHSV-4 

Identified as AHSV-4 by Seg-2 specific real time RT-PCR

Ethiopia

 

Debre Zeit

 

Sample taken
24 June 2010
Equine Blood

 Isolated in KC cells at IAH-P

Shelf  C7,

box  C7β,

rows E1-F10

 

Added to collection 

25 Aug 2010
ETH34/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute

19 Debre Zeit

Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No. 
A72/10 34
 
ETH2010/04

AHSV-4 

Identified as AHSV-4 by Seg-2 specific real time RT-PCR

Ethiopia

Gotia 

 

Sample taken
24 June 2010
Equine Blood

 Isolated in KC cells at IAH-P

Shelf  C7

Box  C7 β,

Rows C1-D10

 

Added to collection 

25 Aug 2010
ETH33/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute

19 Debre Zeit

Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No. 
A72/10 33
ETH2010/03

AHSV-4 

Identified as AHSV-4 by Seg-2 specific real time RT-PCR

Ethiopia

 

Wonji 

 

Sample taken

21 June 2010
Equine Blood

 Isolated in KC cells at IAH-P

Shelf  C7,

box  C7β

rows A1-B10

 

Added to collection 

25 Aug 2010
ETH23/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute

19 Debre Zeit

Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No. 
A72/10 23
 

KEN2007/02

AHSV-4

 

Identified  by serotype specific RT-PCR against Seg 2and confirmed by sequencing

Kenya

 

Sample taken October 2007

Equine

Isolated from homogenised organs,

 passed on Vero cell culture at CVRL Dubai

 

Isolated

22nd Jan 2008

Shelf A8

Box A8 γ

Rows I1-J10

 

 (added to collection on 4thApril 2008)

D1816/07

Vero2

 

[IAH/KC1]

 

 Related to isolate KEN2007/01 but different passage history

Ulli Wernery

CVRL Dubai

 

 

*
Duplicate vial positions I10 (G) 
sent to Marta Vigo Martin, Algete Spain on 15th Dec 2009 under MTA
*

 

KEN2007/01 AHSV-4

Identified  by serotype specific RT-PCR

Kenya

 

Sample taken October 2007

Equine

Isolated from homogenised organs,

 passed on Vero cell culture at CVRL Dubai

Shelf A8,

 Box A8 γ,

 Rows E1-F10

 

Added to collection on  15th Jan 2008
D1816/07

V2 [IAH V2]

IOsolate provided by Ulli Wernery

CVRL Dubai

 

Duplicate sample from freezer A & B provided to Kasia Bankowska, IAH  11th July 2008

Related to isolate KEN2007/02 but different passage history

 

 

SPA1988/01 AHSV-4 Spain 1988       BHK2      

SPA1987/02

AHSV-4

Spain

 

Shelf B7,

box B7α,

rows E1- F10

 

(added to collection

12th Dec 2008)

 

BHK4

[IAH/BHK1]

 

 

 

SPA1987/01 AHSV-4

Spain

 (Madrid)

Horse

(named Ricarda)

 

(isolation date thought to be 21st Apr1989)

Shelf B7,

box B7α,

rows C1- D10

 

(added to collection

12th Dec 2008)

 

Blood

 

MB1 BHK 2

[IAH/BHK1]

     
RSA1938/01 AHSV-4

Confirmed as AHSV-4 single serotype by real-time Seg-2 specific RT-PCR
Vryheit Natal
RSA

1938
    Vryheit vaccine strain original vaccine strain with up to 120 passages in mice

[IAH/BHK5]
  McIntosh 1958

Note:  Plaque cloned x3
 (
O'Hara et al 1998)

strain plaque cloned three times at IAH in BHK cell monolayers during 1991
AHSV - 5

RSArrah/05

AHSV

Type-5

 

 

Shelf B10,

 box B10 γ,

row E1-F10

 

Added to collection on 22 Sept 2009

reference strain of AHSV-5

 

(VH)

(MB3)

[IAH/BHK5]

Dr Baltus Erasmus,

 

OVI South Africa

(1988)

 

Howell (1962)

AHSV - 6

RSArrah/06

AHSV

Type-6

 

 

Shelf B10,

 box B10 γ,

row G1-H10

 

Added to collection on 22 Sept 2009

reference strain of AHSV-6

 

(114)

(MB3)

[IAH/BHK3]

Dr Baltus Erasmus,

 

OVI South Africa

(1988)

 Contents

 

Howell (1962)

ETH2010/19

AHSV

 

Identified as AHSV by Seg-7 group-specific

 real time RT-PCR

 

 

Typed  as AHSV-6 by sequencing of Seg-2

Ethiopia,

 

Yerer,

 

  Sample taken

21 June 2010
From, equine blood

 Isolated in KC cells at IAH-P

Shelf C9,

box  C9α,

rows C1-D10

 

Added  to collection on  31 Aug 2010
ETH08/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH StarsNo.
A72/10 08
 
AHSV - 7

KENrrah/07

AHSV

Type-7

 

 

Shelf B10,

 box B10 γ,

row I1-J10

 

Added to collection on 22 Sept 2009

reference strain of AHSV-7

 

(Karen)

(MB4)

[IAH/BHK3]

Dr Baltus Erasmus,

 

OVI South Africa

(1988)

Howell (1962)

ETH2010/09

AHSV-7

 

Identified as AHSV-7 by Seg-2 type-specific

 real time RT-PCR 

Ethiopia NVI, ETHIOPIA Vaccine strain

Shelf  C7,

box  C7γ,

rows C1-D10

 

 

Added to collection

 26 Aug 2010
ETH73/10

Vero

 

[IAH/V1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No. 
A72/10 73

SEN2007/06

AHSV 7

 

Typed by serotype specific RT-PCR

Guede Bousso Village Senegal

 

September 2007

Lung from male horse isolated in Vero cells

in Senegal

Shelf A8,

box A8γ,

rows C1-D10

 

Added to collection on 15th Jan 2008

5

[V1/IAH V2]

Dr Malik Faye, Dakar,

Senegal

 

[A117/07 5]

*

Duplicate sample from freezer A & B provided to Kasia Bankowska, IAH  11th July 2008

*
Duplicate vial positions D9 (G) sent to Marta Vigo Martin, Algete Spain on 15th Dec 2009 under MTA
*

AHSV - 8

RSArrah/08

AHSV

Type-8

Onderstepoort

South Africa

 

(10th April 1960)

horse-2762

 

(virus isolated at OVI, South Africa)

Shelf B10,

 box B11α,

row A1-B10

 

Added to collection on 22 Sept 2009

reference strain of AHSV-8

 

(18/60)

Spleen

 

(MB3)

[IAH/BHK1]

Dr Baltus Erasmus,

 

OVI South Africa

(1988)

Vial position G3, from freezer A and B, were supplied to the Estonia Veterinary and Food Laboratory,

28th July 2009 

 

Howell (1962)

ETH2010/11

AHSV-8 

 

Identified as AHSV-8 by

Seg-2 type-specific

 real time RT-PCR 

Ethiopia

 

Debre Zeit 

 

Sample taken

25 June 20100
Equine Blood

Isolated in  KC cells at IAH

Shelf  C7

box  C7γ,

rows G1-H10

 

Added to collection

 26 Aug 2010
ETH37/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No. 
A72/10 37
ETH2010/10

AHSV-8 

 

Identified as AHSV-8 by

Seg-2 type-specific

 real time RT-PCR

Ethiopia,

 

Filtino

 

Sample taken

24 June 20100
Equine Blood

Isolated in  KC cells at IAH

Shelf  C7,

box  C7γ, rows E1-F10

 

Added to collection

 26 Aug 2010
ETH31/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No. 
A72/10 31
 

AHSV - 9

PAKrrah/09

AHSV

Type-9

Pakistan Animal Husbandry Institute, Peshawar

 

(10th Dec 1959)

Horse [after inoculation with samples from donkeys]

 

(virus isolated at OVI, South Africa)

Shelf B10,

 box B11α,

row C1-D10

 

Added to collection on 22 Sept 2009

reference strain of AHSV-9

 

(7/60)

Spleen

 

(MB3)

[IAH/BHK6]

Dr Baltus Erasmus,

 

OVI South Africa(1988)

Spare sample 
position C1g freezer B
*
1 ml Sample from position C1g (A freezer only) 
sent to Pfizer/Fort Dodge (Joan de Plana) 
Spain 6th Sept 2010.  Corresponding vial in freezer B
 was not removed 
*

Note: sample tested negative for BTV (Seg-1) and EHDV (Seg-9)  real-time RT-PCR, and negative for FMDV by 3D assay and 5'UTR assay

Howell (1962)

SENvvvv/09

AHSV-9

 

(vaccine strain

- type

AHSV Type-9

by RT-PCR and

sequence analyses 
of Seg-2)

Senegal

 

9 vials

Shelf E18 freezer A 

 

(added to collection

27th Sept 2007)

 
Monoequipeste 
vaccine Contre
la peste equine 
Serotype 9
Flacon de 2 doses
Voie sous cutanee
A conserver a
 +4oc/20oc
(usage veterinaire)
Lot no. M-0107
Util.av Fev. 2009
ISRA vaccines 

Original bottled vaccine from supplier

 

 

Yaya Thiongane 
Laboratoire National
 de l’elevage 
Et de Recherches
 Veterinaires
Bp 2057
Dakar Hann Senegal
 
(used to generate 
SENvvv1/09)
 

SENvvv1/09

AHSV-9

 

(vaccine strain

- type

confirmed by

RT-PCR and 
sequence analyses 
of Seg-2)

Senegal

 

Shelf E1

Box E1 β,

rows E1-F10,

 

(added to collection on  27th June 2007) 
Monoequipeste 
vaccine Contre
la peste equine 
Serotype 9
Flacon de 2 doses
Voie sous cutanee
A conserver a
 +4oc/20oc
(usage veterinaire)
Lot no. M-0107
Util.av Fev. 2009
ISRA vaccines 

Commercial vaccine

[IAH/BHK1]

Yaya Thiongane 
Laboratoire National
 de l’elevage 
Et de Recherches
 Veterinaires
Bp 2057
Dakar Hann Senegal
 
(derived from 
SENvvvv/09 )

Virus confirmed as  AHSV

Type-9 by RT-PCR and

sequence analysis of Seg-2

ETH2010/18

AHSV-9 

 

Identified as AHSV-9 by Seg-2 type-specific

real time RT-PCR 
Ethiopia

NVI, Ethiopia

 

 Vaccine strain

Shelf C9,

box  C9α,

rows A1-B10

 

Added to collection
31Aug 2010
ETH75/10

Vero

 

[IAH/V1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No.

A72/10 75
 
ETH2010/17

AHSV-9 

 

Identified as AHSV-9 by Seg-2 type-specific

real time RT-PCR
Ethiopia Isolated  and
Grown in Vero cells at NVI, Ethiopia

Shelf C8,

box  C8γ,

rows I1-J10

 

Added to collection
31Aug 2010

ET H56/10

(25/06/10)

Vero

 

[IAH/V1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No.

A72/10 56
 
ETH2010/16

AHSV-9 

 

Identified as AHSV-9 by Seg-2 type-specific

real time RT-PCR

Ethiopia

 

Dembie, 

 

Sample taken

3rd Jul 2010

Equine Lung

Horse died of AHSV like symptoms

 Isolated in KC cells at IAH

Shelf C8

Box C8γ,

rows G1-H10

 

Added to collection
31Aug 2010
ET H70/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No.

A72/10 70
Note:  only 9 vials of glycerol
 in A and B
ETH2010/15

AHSV-9 

 

Identified as AHSV-9 by Seg-2 type-specific

real time RT-PCR

Ethiopia,

 

Debre Zeit,

 

Sample taken
15 June 2010

Equine Lung

Horse died of AHSV like symptoms

 Isolated in KC cells at IAH-

Shelf C8,

box C8 γ,

rows E1-F10

 

Added to collection
27Aug 2010
ET H68/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No.

A72/10 68
Note:  only 9 vials of glycerol
in A and B
ETH2010/14

AHSV-9 

 

Identified as AHSV-9 by Seg-2 type-specific

real time RT-PCR

Ethiopia,

 

Godino

 

Sample taken
10 June 2010

Equine Lung

Horse died of AHSV like symptoms

 Isolated in KC cells at IAH-P

Shelf C8,

box C8 γ,

rows C1-D10

 

Added to collection
27Aug 2010
ET H66/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No.

A72/10 66
Note:  only 9 vials of glycerol
in A and B
ETH2010/13

AHSV-9 

 

Identified as AHSV-9 by Seg-2 type-specific

real time RT-PCR

Ethiopia,

 

Akaki 

 

Sample taken
21 June 2010
From equine Blood

Isolated in KC cells at IAH

Shelf C8,

box  C8 γ,

rows A1-B10

 

Added to collection
27Aug 2010
ET H15/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No.

A72/10 15
Note: only 900ul per tube
ETH2010/12

AHSV-9 

 

Identified as AHSV-9 by Seg-2 type-specific

real time RT-PCR

Ethiopia,

Adama 

Sample taken
29 June 2010
From equine Blood

Isolated in KC cells at IAH

Shelf C7

Box  γ

Rows I1-J10

 

Added to collection
27Aug 2010
ETH49/10 [IAH/KC1]

Esayas Gelaye, National Veterinary Institute, 19 Debre Zeit, Ethiopia

 

Samples arrived 22nd July 2010

 

IAH Stars No.

A72/10 49
 
GAM2009/04 AHSV 9

Identified by
 Seg-2 specific RT-PCR and sequencing

Gambia

 

Village Katana Omar

-c-

 

Sample taken 22nd Oct 2009

Equine blood

 

Isolated at IAH Pirbright

Shelf B14

Box B14 γ

Rows C1-D10

 (added to collection on  1st Mar 2010)

  [IAH/KC1/BHK1]

Philip Ivens

Nixon Equine Vets

Buckingham

 

[A89/09 18]

From male horse age 3 unvaccinated

No clinical signs

Derived from GAM2009/02

GAM2009/03

AHSV 9

Identified by
 Seg-2 specific RT-PCR and sequencing

Gambia

 

Village Katana Omar

-c-

 

Sample taken 22nd Oct 2009

Equine blood

 

Isolated at IAH Pirbright

Shelf B14

Box B14 γ

Rows A1-B10

 

(added to collection on  1st Mar 2010)

[IAH/KC2/BHK1]

Philip Ivens

Nixon Equine Vets

Buckingham

 

[A89/09 12]

From female horse age 3 unvaccinated

No clinical signs

Derived from  GAM2009/01

GAM2009/02 AHSV 9

Identified by
 Seg-2 specific RT-PCR and sequencing

Gambia

 

Village Katana Omar

-c-

 

Sample taken 22nd Oct 2009

Equine blood

 

 Isolated at IAH Pirbright

 Shelf B14

Box B14 γ,

Rows C1-D10

 

(added to collection on  1st Mar 2010)

  [IAH/KC1]

Philip Ivens

Nixon Equine Vets

Buckingham

 

[A89/09 18]

From male horse age 3 unvaccinated

No clinical signs

Used to generate GAM2009/04
GAM2009/01 AHSV 9

Identified by
 Seg-2 specific RT-PCR and sequencing

 Gambia

 

Village Katana Omar

-c-

 

Sample taken 22nd Oct 2009

Equine blood

 

Isolated at IAH Pirbright

 Shelf B14,

Box B14 γ,

Rows A1-B10

 (added to collection on  1st Mar 2010)

[IAH/KC2]

Philip Ivens

Nixon equine vets

Buckingham

 

[A89/09 12]

From female horse age 3 unvaccinated
No clinical signs

Used to generate GAM2009/03

KEN2006/01

AHSV
Type 9
 
Identified by 
RT-PCR 
and 
VNT
Kenya 
 
The horse
 died on
25th July 2006 
Isolated from
 a foal, which
 died in Nairobi. 
Typical lesions 
of AHS in 
the lung
 
Isolated in 
Dubai CVRL 

Shelf D3,

box D3 β,

rows I1-J10

 

(added to collection  14th Nov 2006)

D1357/06

Ex trachea

 
Isolated in 
Vero cells 
 

V1

[IAH/E1/BHK3]

 

Dr. Habil Ulrich Wernery
Scientific Director
Central Veterinary

Research Laboratory
P.O. Box 597, Dubai  U.A.E.

 

Received 25th Sept2006

 

(A86/06 1)

From the same animal as KEN2006/02

 

Duplicate sample from freezer

A & B provided to Kasia Bankowska, IAH 

11th July 2008

 

» Contents

KEN2006/02

AHSV
Type 9
 
Identified by 
RT-PCR 
and 
VNT
Kenya 
 
The horse
 died on
25th July 2006 
Isolated from
 a foal, which
 died in Nairobi. 
Typical lesions 
of AHS in 
the lung
 
Isolated in 
Dubai CVRL 

Shelf D3,

box D3 γ,

rows A1-B10

 

(added to collection  14th Nov 2006)

D1357/06

Ex lung

 
Isolated in 
Vero cells 
 

V1

[IAH/E1/BHK3]

 

Dr. Habil Ulrich Wernery
Scientific Director
Central Veterinary

Research Laboratory
P.O. Box 597, Dubai  U.A.E.

 

(A86/06 2)

From the same animal as 
KEN2006/01
SEN1998/01

AHSV-9

Identified as AHSV-9 by Seg-2 specific

 Real time RT-PCR  and sequencing

Senegal Equine  Isolated through Vero cells at ANSES

Shelf  C11,

box  C11 α,

rows I1-J10

 

Added to collection on  17th Dec 2010    
  V2
[IAH/BHK1]

 Corinne Sailleau

ANSES (AFFSA) France

 

 

 

 

 

 

Miscellaneous AHSV samples

derived from

 isolate number

 Storage location

Notes

 

Misc  box, Freezer A,

Shelf E18, Box E18 β position G4.

AHS ring-trial samples

: 12-08 - Freezer A, shelf E18, Miscellaneous box A, position G4.

 

Misc  box, Freezer A,

Shelf E18, Box E18 β position G3

AHS ring-trial samples

12-07 - Freezer A, shelf E18, Miscellaneous box A, position G3.

 

Misc  box, Freezer A,

Shelf E18, Box E18 β position G2

AHS ring-trial samples

12-10 - Freezer A, shelf E18, Miscellaneous box A, position G2.

 

Misc  box, Freezer A,

Shelf E18, Box E18 β position G1

AHS ring-trial samples

12-09 - Freezer A, shelf E18, Miscellaneous box A, position G1.

 

 

 

 

References

 

Howell,  P. G. (1962) The isolation and identification of further antigenic types of African horsesickness virus. Onderstepoort Journal of Veterinary Research 29, 139-149

 

McIntosh, B.M. (1958) Immunological types of horse sickness virus and their significance in immunisation.  Onderstepoort Journal of Veterinary Research 27, 465 - 513.

 

O'Hara, R. S., Meyer, A. J., Burroughs JN, Pullen, L., Martin, L.‑A. and Mertens, P. P. C. (1998)  Development of a mouse model system and identification of individual genome segments of African horse sickness virus, serotypes 3 and 8 involved in determination of virulence: abstract.  Archives of Virology  Supplement 14, 259‑279.

 

 

 

 

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