ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Equine encephalosis virus (EEV)

in the dsRNA virus collection at  IAH Pirbright 

 

Return to Virus Collection Contents

 

Untyped EEV
isolates
EEV-1 EEV-2 EEV-3 EEV-4 EEV-5 EEV-6 EEV-7

 

 Family: Reoviridae, Genus: Orbivirus, Species: Equine encephalosis virus   (EEV)  

IAH dsRNA virus collection number: [Country code year / sample number]

Virus Species

&

Serotype

 

(Reference to serotype numbers)

 

 (identification method)

Place of original sample eg country town

 [grid reference] (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 (Date added to collection)

 (Suppliers Isolate Designation)

Original sample material, Adapted to which cell types 

 

(Passage history of sample)

[IAH passage history]

* Original sample supplied to the collection by person / institution [IAH, ISIS sample number]

Comments

 (References)

 &

 [ accession numbers]

 

  (Explanation of EEV serotype numbers)

Equine encephalosis virus serotype 4  (EEV-1) 

RSA1967/03

(reference strain)

EEV Type 1

Kimberley,

Northern Cape,

Republic of South Africa

 

(1967)  

From horse organ suspension

added to collection

2nd October  2007

(Cascara

SM, 2 BHK, (19/03/80))

Originally isolated from organ suspension

 

SM2/BHK2

 [IAH/BHK5]

original strain supplied by Onderstepoort Veterinary Institute

Derived from RSA1967/02

Howell et al 2002

RSA1967/02

EEV Type 1

Kimberley,

Northern Cape,

Republic of South Africa

 

(1967)

From horse organ suspension

Shelf A18, boxA18γ,

rows C1-D10

 

Added to collection on 12th Dec 2008

(Cascara

SM, 2 BHK, (19/03/80))

Originally isolated from organ suspension

 

SM2/BHK2

 [IAH/BHK4]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

used to

 generate RSA1967/03

added to collection

2nd October  2007

 

Only 5 duplicate vials only in pDAM

Vial C1(p) (duplicate) sent to Piet  van Rijn, Lelystad The Netherlands  3rdSept 2009

*  

Howell et al 2002

RSA1967/01

EEV Type 1

Kimberley,

Northern Cape,

Republic of South Africa

 

(1967)

From horse organ suspension

EEV box

 

added to collection

2nd July 2002

(Cascara

SM, 2 BHK, (19/03/80))

Originally isolated from organ suspension

 

SM2/BHK2

 [IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

10 vials only, in pDAM

(stored in freezer A, in EEV box )

Howell et al 2002
Equine encephalosis virus serotype 2 (EEV-2) 

RSA1971/06

(reference strain)

EEV type 2

Namboomspruit, Northern Province,

Republic of South Africa

 

(1971)

From horse blood

Shelf A18, boxA18γ,

rows E1-F10

 

Added to collection on

12 Dec 2008

(Gamil, M9/71

4BHK, 1 Vero,

24/04/93)

BHK4/Vero1

[IAH/BHK4]

original strain supplied by Onderstepoort Veterinary Institute

Vial E1(P) (duplicate) sent to Piet  van Rijn Lelystad The Netherlands  3rdSept 2009

Derived from

RSA1971/05

 

Howell et al 2002

 

RSA1971/05

 

EEV type 2 Namboomspruit, Northern Province,

Republic of South Africa

 

(1971)

From horse blood

added to collection

2nd Oct 2007

(Gamil, M9/71

4BHK, 1 Vero,

24/04/93)

BHK4/Vero1 [IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

used to

 generate

RSA1971/06

 

5 duplicate vials only in pDAM

Howell et al 2002

RSA1971/04 EEV type 2 Namboomspruit, Northern Province,

Republic of South Africa

 

(1971)

From horse blood

EEV box

 

added to collection

2nd July 2002

(Gamil, M9/71

4BHK, 1 Vero,

24/04/93)

BHK4/Vero1 [IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

10 vials only, in pDAM

(stored in freezer A, in EEV box )

Howell et al 2002

Equine encephalosis virus serotype 3 (EEV-3) 
RSA1974/06

(reference strain)

EEV Type 3

Onderstepoort, Gauteng

Republic of South Africa

 

(1974)

From horse

blood

Shelf A18, boxA18γ, rows G1-H10

Added to collection on 12th Dec 2008

 (Kaalplas

4BHK,

 (06/07/77))

BHK4

 [IAH/BHK4]

original strain supplied by Onderstepoort Veterinary Institute

Vial G1(p) (duplicate) sent to Piet  van Rijn Lelystad The Netherlands  3rdSept 2009

Derived from

RSA1974/05

 

(originally added to collection

2nd October 2007)

 

Howell et al 2002

GAM2009/06 EEV
 Type 3

identified by type specific real-time RT-PCR and sequence analyses
Ethiopia  Village Jallow Kunda

sampled 23/10/09
From a donkey, age 2.5, mare, not vaccinated

owner Sara Gaye,

 Shelf C15,

box C15 Beta,

rows E1 F10

 

Added to collection

22 September

2011

  [TPI/ BHK2]

Ct on BHK1 of 15/16.

  Stars No. A89/09/62

MTA - Contact AMRG prior to distribution

Vials E10 were removed from freezer A & B.
GAM2009/05 EEV
 Type 3

identified by type specific real-time RT-PCR and sequence analyses
Ethiopia  Village Jallow Kunda

sampled 23/10/09,
from a donkey, age 4 stallion, not vaccinated,

owner
Alieu Mbye,

 Shelf C15,

box C15 Beta,

rows C1D10

 

Added to collection

20 September

2011

[TPI/ BHK2]

Ct value for BHK1 = 28
Stars No. A89/09/ 56

MTA - Contact AMRG prior to distribution

RSA1974/05

EEV Type 3

Onderstepoort, Gauteng

Republic of South Africa

 

(1974)

From horse

blood

added to collection

2nd October 2007

 (Kaalplas

4BHK,

 (06/07/77))

BHK4

  [IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

used to generate

RSA1975/06

 

5 duplicate vials only in pDAM

Howell et al 2002

RSA1974/04

EEV Type 3

Onderstepoort, Gauteng

Republic of South Africa

 

(1974)

From horse

blood

EEV box

 

added to collection

2nd July 2002

 (Kaalplas

4BHK,

 (06/07/77))

BHK4

  [IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

10 vials only in pDAM

 

(In freezer A in EEV box)

Howell et al 2002

ISR2009/21

EEV-3

 

 Identified as EEV-3 by Seg-10, Seg-9 and Seg-2 specific  real time RT-PCRs 

Israel

sampled on
27th Nov 2009
Equine blood Isolated in Vero cells at the Koret Veterinary School, Israel

Shelf  C11

Box  C11 β

Rows C1-D10

 

Added to collection on
17th Dec 2011
from horse 'H8' V6

[IAH/V1]

Eyal Klement

Koret veterinary school, Israel

 

 Samples arrived 24th Nov 2010

  

IAH Stars No.

A113/10 03 and

A113/10 04

Isolated from a horse showing clinical signs (fever [39.70C] inappetance, colic

Aharonson-Raz et al (2011)


Date of first clinical signs
 
26th Nov 2009

[Seg-10: HQ441247]
ISR2009/20

EEV-3

 

 Identified as EEV-3 by Seg-10, Seg-9 and Seg-2 specific  real time RT-PCRs 

Israel

sampled on
3rd Nov 2009
Equine blood

 Isolated in Vero cells at the Koret Veterinary School, Israel

Shelf  C11,

box  C11 β,

rows A1-B10

 

Added to collection on
17th Dec 2011
from horse 'H3' V6

[IAH/V1]

Eyal Klement

Koret veterinary school, Israel

 

Samples arrived 24th Nov 2010

  

IAH Stars No.

A113/10 01 and A113/10 02

Isolated from a horse showing clinical signs (fever [400C] and  inappetance)

Date of first clinical signs
 
30th Oct 2009

Aharonson-Raz et al (2011)


[Seg-10:
HQ441246]
Equine encephalosis virus serotype 4 (EEV-4) 

RSA1976/03

(reference strain)

 

 

EEV Type 4

Colesberg, Western Cape,

Republic of South Africa

 

(1976)

From horse

foetal liver / spleen

Shelf A18, boxA18γ,

rows A1-B10

 

Added to collection on 12th Dec 2008

(Bryanston M8/76)

 

3BHK

(28/06/76))

Originally isolated from foetal liver / spleen

 

BHK3

 [IAH/BHK4]

original strain supplied by Onderstepoort Veterinary Institute

Derived from

RSA1976/02 (original sample added to collection  2nd Oct  2007)

Vial A1(g) (duplicate) sent to Piet  van Rijn Lelystad The Netherlands  3rdSept 2009

>>Contents

 

Howell et al 2002

RSA1976/02

EEV Type 4

Colesberg, Western Cape,

Republic of South Africa

 

(1976)

From horse

foetal liver / spleen

added to collection

2nd October  2007

(Bryanston M8/76)

 

3BHK

(28/06/76))

 

Originally isolated from foetal liver / spleen

 

BHK3

[IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

Derived from

RSA1976/01

 

Used to generate

RSA1976/03

 

only 5 duplicate vials in pDAM

 

 

Howell et al 2002

RSA1976/01

EEV Type 4

Colesberg, Western Cape,

Republic of South Africa

 

(1976)

From horse

foetal liver / spleen

EEV box

 

added to collection

2nd July  2002

(Bryanston M8/76)

 

3BHK

(28/06/76))

 

Originally isolated from foetal liver / spleen

 

BHK3

 [IAH/BHK3]

 

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

10 vials only, in pDAM

 

(stored in freezer A in EEV box)

Howell et al 2002
Equine encephalosis virus serotype 5 (EEV-5) 
RSA1976/06
(reference strain)

(Previously identified incorrectly as RSA1974/03)

EEV Type 5

Johannesburg, Gauteng

Republic of South Africa

 

(1976)

From horse

blood

Shelf A18, boxA18γ, rows I1-J10

Added to collection on 12the Dec 2008

 (Kyalami

4BHK,

(03/07/85)

BHK4

 [IAH/BHK4]

original strain supplied by Onderstepoort Veterinary Institute

Vial I1(g) (duplicate) sent to Piet  van Rijn Lelystad The Netherlands  3rdSept 2009

Derived from

RSA1974/02

 

(originally added to collection

2nd Oct 2007)

Howell et al 2002

RSA1976/05

 

(Previously identified incorrectly as RSA1974/02)

EEV Type 5

Johannesburg, Gauteng

Republic of South Africa

 

(1976)

From horse

blood

added to collection

2nd October  2007

 (Kyalami

4BHK,

(03/07/85)

BHK4

  [IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

used to

 generate

RSA1974/03

 

 

Only 5 duplicate vials only in pDAM

Howell et al 2002

RSA1976/04

 

(Previously identified incorrectly as RSA1974/01)

EEV Type 5

Johannesburg, Gauteng

Republic of South Africa

 

(1976)

From horse

blood

added to collection

2nd July 2002

 (Kyalami

4BHK,

(03/07/85)

BHK4

  [IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

10 vials only in pDAM

 

(In freezer A in EEV box)

Howell et al 2002
Equine encephalosis virus serotype 6 (EEV-6) 

RSA1991/03

(reference strain)

EEV Type 6

Potchefstroom, North West Province,

Republic of  South Africa

 

(1991)

From horse

blood

Shelf B7, boxB7α,

rows A1-B10

 

Added to collection on 12th Dec 2008

 (Potchefstroom, Else EP8/91)

 

1Vero, 1 CER (26/08/91)) 

Vero1/*CER1

 [IAH/BHK4]

original strain supplied by Onderstepoort Veterinary Institute

Vial A1(p) (duplicate) sent to Piet  van Rijn Lelystad The Netherlands  3rdSept 2009

Derived from RSA1991/02

 

(originally added to collection

2nd Oct  2007)

Gerdes & Pieterse (1993)

 

Howell et al 2002

RSA1991/02

EEV Type 6

Potchefstroom, North West Province,

Republic of  South Africa

 

(1991)

From horse

blood

added to collection

2nd October  2007

 (Potchefstroom, Else EP8/91)

 

1Vero, 1 CER (26/08/91)) 

Vero1/*CER1

 [IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

used to generate

RSA1991/03

 

 

only  5 duplicate vials in pDAM

Gerdes & Pieterse(1993)

 

Howell et al 2002

RSA1991/01

EEV Type 6

Potchefstroom, North West Province,

Republic of  South Africa

 

(1991)

From horse

blood

EEV box

 

added to collection

2nd July 2002

 (Potchefstroom, Else EP8/91)

 

1Vero, 1 CER (26/08/91)) 

Vero1/*CER1

 [IAH/BHK3]

Supplied by Onderstepoort Veterinary Institute

 

(received

3rd Sept 2001)

10 vials only in pDAM

 

(In freezer A in EEV box)

Gerdes & Pieterse(1993)

 

Howell et al 2002

Equine encephalosis virus serotype 7 (EHDV-7)
                   

* CER = Chicken /BHK hybridoma l

 

Links to References

 Karin Aharonson-Raz, Amir Steinman, Velizar Bumbarov, Sushila Maan, Narender Singh Maan, Kyriaki Nomikou, Carrie Batten, Christiaan Potgieter, Yuval Gottlieb, Peter Mertens, Eyal Klement (2011) The Isolation and Characterization of Equine Encephalosis Virus in Israel  (submitted).

 

Howell, P. G., Groenewald, D., Visage, C. W., Bosman, A. M., Coetzer, J. A., Guthrie, A. J. (2002) The classification of seven serotypes of equine encephalosis virus and the prevalence of homologous antibody in horses in South Africa . Onderstepoort J. Vet Res 69 79-93.

 

Gerdes GH, Pieterse LM.(1993) The isolation and identification of Potchefstroom virus: a new member of the equine encephalosis group of 
orbiviruses. J S Afr Vet Assoc. 64:131-132.

 

 

 

 

 

 

 

 

 

 

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