|
Family:
Reoviridae, Genus: Orbivirus, Species:
Equine encephalosis
virus (EEV) |
|
IAH dsRNA virus collection number: [Country code year / sample number] |
Virus Species
&
Serotype
(Reference to serotype numbers)
(identification method) |
Place of original sample eg country town
[grid reference] (date original sample was taken) |
Isolated from which host species e.g cow, sheep, etc
(Date of virus isolation, where, by whom) |
Position of storage in reference freezers
(Date added to collection) |
(Suppliers Isolate Designation) |
Original sample material, Adapted to which cell types
(Passage history of sample)
[IAH passage history] |
* Original sample supplied to the collection by person / institution
[IAH, ISIS sample number] |
Comments |
(References)
&
[ accession numbers]
(Explanation
of EEV serotype numbers) |
|
Equine encephalosis
virus serotype 4 (EEV-1) |
|
RSA1967/03
(reference strain) |
EEV Type 1 |
Kimberley,
Northern Cape,
Republic of South Africa
(1967) |
From horse organ
suspension |
added to
collection
2nd October 2007 |
(Cascara
SM, 2 BHK, (19/03/80)) |
Originally
isolated from organ suspension
SM2/BHK2
[IAH/BHK5] |
original strain supplied by Onderstepoort Veterinary
Institute |
Derived from
RSA1967/02 |
Howell et al 2002 |
|
RSA1967/02 |
EEV Type 1 |
Kimberley,
Northern Cape,
Republic of South Africa
(1967) |
From horse organ
suspension |
Shelf A18, boxA18γ,
rows C1-D10
Added to
collection on 12th Dec 2008 |
(Cascara
SM, 2 BHK, (19/03/80)) |
Originally
isolated from organ suspension
SM2/BHK2
[IAH/BHK4] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
used to
generate
RSA1967/03 added to
collection
2nd October 2007
Only 5 duplicate vials only in pDAM
*
Vial C1(p)
(duplicate) sent to Piet van Rijn, Lelystad The Netherlands 3rdSept
2009
*
Howell et al 2002 |
|
RSA1967/01 |
EEV Type 1 |
Kimberley,
Northern Cape,
Republic of South Africa
(1967) |
From horse organ
suspension |
EEV box
added to
collection
2nd July 2002 |
(Cascara
SM, 2 BHK, (19/03/80)) |
Originally
isolated from organ suspension
SM2/BHK2
[IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
10 vials only, in pDAM (stored in
freezer A, in EEV box ) |
Howell et al 2002 |
|
Equine encephalosis virus serotype 2
(EEV-2) |
|
RSA1971/06
(reference strain) |
EEV type 2 |
Namboomspruit, Northern Province,
Republic of South
Africa
(1971) |
From horse blood |
Shelf A18, boxA18γ,
rows E1-F10
Added to
collection on
12 Dec 2008 |
(Gamil, M9/71
4BHK, 1 Vero,
24/04/93) |
BHK4/Vero1
[IAH/BHK4] |
original strain supplied by Onderstepoort Veterinary
Institute |
*
Vial E1(P)
(duplicate) sent to Piet van Rijn Lelystad The Netherlands 3rdSept
2009
*
Derived from
RSA1971/05
Howell et al 2002 |
|
RSA1971/05
|
EEV type 2 |
Namboomspruit, Northern Province,
Republic of South
Africa
(1971) |
From horse blood |
added to
collection
2nd Oct 2007 |
(Gamil, M9/71
4BHK, 1 Vero,
24/04/93) |
BHK4/Vero1 [IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
used to
generate
RSA1971/06
5 duplicate vials only in pDAM |
Howell et al 2002 |
|
RSA1971/04 |
EEV type 2 |
Namboomspruit, Northern Province,
Republic of South
Africa
(1971) |
From horse blood |
EEV box
added to collection
2nd July 2002 |
(Gamil, M9/71
4BHK, 1 Vero,
24/04/93) |
BHK4/Vero1 [IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
10 vials only, in pDAM (stored in
freezer A, in EEV box ) |
Howell et al 2002 |
|
Equine encephalosis
virus serotype 3 (EEV-3) |
RSA1974/06
(reference strain) |
EEV Type 3 |
Onderstepoort,
Gauteng Republic of South Africa
(1974) |
From horse
blood |
Shelf A18, boxA18γ, rows G1-H10
Added to
collection on 12th Dec 2008 |
(Kaalplas
4BHK,
(06/07/77)) |
BHK4
[IAH/BHK4] |
original strain supplied by Onderstepoort Veterinary
Institute |
Vial G1(p)
(duplicate) sent to Piet van Rijn Lelystad The Netherlands
3rdSept
2009
*
Derived from
RSA1974/05
(originally added to
collection
2nd October 2007)
Howell et al 2002 |
|
GAM2009/06 |
EEV
Type 3
identified by type specific real-time RT-PCR and sequence analyses |
Ethiopia Village – Jallow Kunda
sampled 23/10/09 |
From a
donkey, age 2.5, mare, not vaccinated
owner – Sara Gaye, |
Shelf C15,
box C15 Beta,
rows E1– F10
Added
to collection
22
September
2011
|
|
[TPI/ BHK2]
Ct on BHK1 of 15/16.
|
|
Stars No.
A89/09/62
MTA - Contact AMRG prior to distribution
Vials E10 were removed from freezer A & B. |
|
GAM2009/05 |
EEV
Type 3
identified by type specific real-time RT-PCR and sequence analyses |
Ethiopia Village – Jallow Kunda
sampled 23/10/09, |
from a donkey, age 4 stallion, not vaccinated,
owner
Alieu Mbye, |
Shelf C15,
box C15 Beta,
rows C1–D10
Added to collection
20 September
2011
|
|
[TPI/ BHK2]
Ct value for BHK1 = 28 |
|
Stars No.
A89/09/ 56
MTA - Contact AMRG prior to distribution |
|
RSA1974/05 |
EEV Type 3 |
Onderstepoort,
Gauteng Republic of South Africa
(1974) |
From horse
blood |
added to
collection
2nd October 2007 |
(Kaalplas
4BHK,
(06/07/77)) |
BHK4
[IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
used to generate
RSA1975/06
5 duplicate vials only in pDAM |
Howell et al 2002 |
|
RSA1974/04 |
EEV Type 3 |
Onderstepoort,
Gauteng Republic of South Africa
(1974) |
From horse
blood |
EEV box
added to
collection
2nd July 2002 |
(Kaalplas
4BHK,
(06/07/77)) |
BHK4
[IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
10 vials only in pDAM
(In freezer A in EEV box) |
Howell et al 2002 |
|
ISR2009/21 |
EEV-3
Identified
as EEV-3 by Seg-10, Seg-9 and Seg-2 specific
real time RT-PCRs
|
Israel
sampled on
27th Nov 2009 |
Equine blood Isolated in Vero cells at the Koret Veterinary School, Israel |
Shelf
C11
Box
C11 β
Rows C1-D10
Added to collection on
17th Dec 2011 |
from horse 'H8' |
V6
[IAH/V1] |
Eyal Klement
Koret veterinary school, Israel
Samples arrived 24th Nov 2010
IAH Stars
No.
A113/10 03 and
A113/10 04
|
Isolated from a horse showing clinical signs (fever
[39.70C]
inappetance, colic
Aharonson-Raz et al (2011)
Date of first clinical signs
26th
Nov
2009
[Seg-10:
HQ441247] |
|
ISR2009/20 |
EEV-3
Identified
as EEV-3 by Seg-10, Seg-9 and Seg-2 specific
real time RT-PCRs
|
Israel
sampled on
3rd Nov 2009 |
Equine blood
Isolated in Vero cells at the Koret Veterinary School, Israel |
Shelf
C11,
box
C11 β,
rows A1-B10
Added to collection on
17th Dec 2011 |
from horse 'H3' |
V6
[IAH/V1] |
Eyal Klement
Koret veterinary school, Israel
Samples arrived 24th Nov 2010
IAH Stars No.
A113/10 01 and A113/10 02
|
Isolated from a horse showing clinical signs (fever
[400C]
and
inappetance)
Date of first clinical signs
30th
Oct 2009
Aharonson-Raz et al (2011)
[Seg-10:
HQ441246] |
|
Equine encephalosis
virus serotype 4 (EEV-4) |
|
RSA1976/03
(reference strain)
|
EEV Type 4 |
Colesberg, Western
Cape,
Republic of South Africa
(1976) |
From horse
foetal liver / spleen |
Shelf A18, boxA18γ,
rows A1-B10
Added to
collection on 12th Dec 2008 |
(Bryanston M8/76)
3BHK
(28/06/76)) |
Originally
isolated from foetal liver / spleen
BHK3
[IAH/BHK4] |
original strain supplied by Onderstepoort Veterinary
Institute |
Derived from
RSA1976/02 (original sample added to
collection 2nd Oct 2007)
*
Vial A1(g)
(duplicate) sent to Piet van Rijn Lelystad The Netherlands 3rdSept
2009
*
>>Contents
Howell et
al 2002 |
|
RSA1976/02 |
EEV Type 4 |
Colesberg, Western
Cape,
Republic of South Africa
(1976) |
From horse
foetal liver / spleen |
added to
collection
2nd October 2007 |
(Bryanston M8/76)
3BHK
(28/06/76))
|
Originally
isolated from foetal liver / spleen
BHK3
[IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
Derived from
RSA1976/01
Used to generate
RSA1976/03
only 5 duplicate vials in pDAM |
Howell et al 2002 |
|
RSA1976/01 |
EEV Type 4 |
Colesberg, Western
Cape,
Republic of South Africa
(1976) |
From horse
foetal liver / spleen |
EEV box
added to
collection
2nd July 2002 |
(Bryanston M8/76)
3BHK
(28/06/76))
|
Originally
isolated from foetal liver / spleen
BHK3
[IAH/BHK3]
|
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
10 vials only, in pDAM
(stored in freezer A in EEV box) |
Howell et al 2002 |
|
Equine encephalosis
virus serotype 5 (EEV-5) |
RSA1976/06
(reference strain)
(Previously identified incorrectly as RSA1974/03) |
EEV Type 5 |
Johannesburg,
Gauteng
Republic of South Africa
(1976) |
From horse
blood |
Shelf A18, boxA18γ, rows I1-J10
Added to
collection on 12the Dec 2008 |
(Kyalami
4BHK,
(03/07/85) |
BHK4
[IAH/BHK4] |
original strain supplied by Onderstepoort Veterinary
Institute |
*
Vial I1(g)
(duplicate) sent to Piet van Rijn Lelystad The Netherlands 3rdSept
2009
*
Derived from
RSA1974/02
(originally added to
collection
2nd Oct 2007)
Howell et al 2002 |
|
RSA1976/05
(Previously identified incorrectly as RSA1974/02) |
EEV Type 5 |
Johannesburg,
Gauteng
Republic of South Africa
(1976) |
From horse
blood |
added to
collection
2nd October 2007 |
(Kyalami
4BHK,
(03/07/85) |
BHK4
[IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
used to
generate
RSA1974/03
Only
5 duplicate vials only in pDAM |
Howell et al 2002 |
|
RSA1976/04
(Previously
identified incorrectly as RSA1974/01) |
EEV Type 5 |
Johannesburg,
Gauteng
Republic of South Africa
(1976) |
From horse
blood |
added to
collection
2nd July 2002 |
(Kyalami
4BHK,
(03/07/85) |
BHK4
[IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
10 vials only in pDAM
(In freezer A in EEV box) |
Howell et al 2002 |
|
Equine encephalosis
virus serotype 6 (EEV-6) |
|
RSA1991/03
(reference strain) |
EEV Type 6 |
Potchefstroom,
North West Province,
Republic of South Africa
(1991) |
From horse
blood |
Shelf B7, boxB7α,
rows A1-B10
Added to
collection on 12th Dec 2008 |
(Potchefstroom,
Else EP8/91)
1Vero, 1 CER (26/08/91))
|
Vero1/*CER1
[IAH/BHK4] |
original strain supplied by Onderstepoort Veterinary
Institute |
*
Vial A1(p)
(duplicate) sent to Piet van Rijn Lelystad The Netherlands
3rdSept
2009
*
Derived from
RSA1991/02
(originally added to
collection
2nd Oct 2007)
Gerdes
& Pieterse (1993)
Howell et al 2002 |
|
RSA1991/02 |
EEV Type 6 |
Potchefstroom,
North West Province,
Republic of South Africa
(1991) |
From horse
blood |
added to
collection
2nd October 2007 |
(Potchefstroom,
Else EP8/91)
1Vero, 1 CER (26/08/91)) |
Vero1/*CER1
[IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
used to generate
RSA1991/03
only 5 duplicate vials in pDAM |
Gerdes
& Pieterse(1993)
Howell et al 2002 |
|
RSA1991/01 |
EEV Type 6 |
Potchefstroom,
North West Province,
Republic of South Africa
(1991) |
From horse
blood |
EEV box
added to
collection
2nd July 2002 |
(Potchefstroom,
Else EP8/91)
1Vero, 1 CER (26/08/91)) |
Vero1/*CER1
[IAH/BHK3] |
Supplied by Onderstepoort Veterinary
Institute
(received
3rd Sept 2001) |
10 vials only in pDAM
(In freezer A in EEV box) |
Gerdes
& Pieterse(1993)
Howell et al 2002 |
|
Equine encephalosis virus
serotype 7
(EHDV-7) |
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