ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 10 (BTV-10)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection Contents

 

 

 Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus (BTV) serotype 10

 *IAH dsRNA virus collection number

 

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 

 (identification method)

Place of original sample eg country town

 [grid reference]

 

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 

 Adapted to which cell types 

 

(Passage history of sample)

 

[IAH passage history]

* Original sample

supplied to the collection

by person / institution

 

[IAH, ISIS sample number]

 

Comments

 References &  

accession numbers  

 RSArrrr/10 

BTV-10

 

(western topotype Reference strain)

Supplied by OVI S. Africa* (received 06/02/83)

NK 

Shelf B4,

box B4α

Rows A1-B10

 

(added to collection  27th May 2005)

(BTV-10

Ref. Strain)

(atten.)

BHK

 

 (BHK5 )

[IAH/BHK1]

 

*

Duplicate samples  A1(g)

 supplied on 19th Dec 2007,

 to JongChul, Jeong Ja Yeon

Agrotech Co., Ltd., 385-6,

ChonChon-Dong, JangAn-Gu,

SuWon-City, KyungKi-Do,

440-330, Korea

Vial B1 (p) A&B sent to

Mehdi El Harrak, Biopharma ,

Morocco  29th June 2009

*

Vial B2 sent to Denis Kolbasov

Russian National Institute of 

Veterinary Virology 29th Sept 2009

*  

Vial A sent to Dr Graham

Abdeljelil, Institut Pasteur

de Tunis 8th March 2011

*  

 

 [Seg-2: AJ585131,

Seg-6: AJ586709,

Maan et al 2004,

Singh et al 2004,

Maan et al 2007]

 

contents

MAR2005/02 BTV-10 Martinique (France)

(Sample taken Dec  2005)
from a
non-vaccinated bovine
Shelf C8,
Box C8α,
 Rows A1-B10  

(added to collection 25/08/2010)

 tube labelled:

BTV-10/06.01 - 21
 [IAH/BHK1 ] Supplied by
Emmanuel Breard,
AFSSA, Maisons Alfort France

23rd July 2010
Distribution restricted by MTA

[S2:  ]
USA2003/02

BTV-10

 

typed by

 Seg-2 RT-PCR

Manatee County FL,

USA

 

(sample taken

21st Oct 2003)

Virus isolated from Cattle blood

 

(multiple serotypes of BTV were isolated from this herd : BTV-2, 3, 10 and unknown serotypes)

 

virus isolated on

2nd Dec 2003

Shelf  D6,

Box D6α

Rows A1-B10

 

Shelf D9,

box D9 gamma

Rows C1-D10

(Added to

collection on

28th of February 2014)

 

Shelf D12,

box D12α

Rows E1-F10

04-280559-2

 

 tube labelled

 280559-2;

IVL YSD BHK-1;

12-2-03 BT

E2/BHK1

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

ZIM2003/06

 

 

BTV

Type 10

 

typed by SNT

Zimbabwe

(Lone Oak)

2003

 

 

(25th Sep 2003)  

Culicoides imicola

Shelf B6, 
Box B6β
Rows G1-H10
 
(added to 
collection 
01 Oct 2005)

(25/08/03)  

(E1/BHK1)

[IAH/BHK2]

[R06/03, No.127]

   

 

USA2002/03 BTV-10

 

(serotyped

by Seg-2 

RT-PCR)

 

Okeechobee County FL, USA

 

(sample taken

30th Oct 2002)

From cattle

 

Sample submitted for export testing, no clinical history given

 

(isolate preparation date 7th Aug 2006)

Shelf E1, 
box E1 ALPHA,

rows E1 - F10

 

(added to collection on 15th June 2007)

03-220082-15

 

(tube labelled: 220082-15;

IVD BHK/1;

8-7-06)

 

From blood

 

E1/BHK1/E1/BHK1

[IAH/ BHK3]

virus isolate supplied by :

 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

nvsluf@aphis.usda.gov

[S2: ]

 

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

repeated passage of original isolate generated virus of low titre, so the original isolate was inoculated into ECE in July 2006, followed by one pass in BHK-21 cells. original typing attempts as one of previous US types were unsuccessful.  Multiple  other serotypes were isolated from this herd (BTV-3, 10 and two isolates of unknown serotype:  see USA2002/02). 

USA1998/08

BTV-10

 

typed by

by Seg-2

RT-PCR

Stanislaus  County CA

USA

 

(sample taken

12th Oct 1998)

Virus isolated from Cattle blood

 

(sample was submitted to USDA for export testing - no clinical history)

 

Isolation date unknown

Shelf D6,

box D6 α

rows I1-J10  

(added to collection 23rd May 2007)

99-1408

 

 tube labelled

 99-1408;

IVD BHK/1;

BT10

E1/BHK1

[IAH/BHK2]

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

DOM1996/01

BTV-10

Identified by SNT

 

Confirmed by type specific Rt-PCR and sequence analysis of Seg-2

Dominican Republic

 

Sample taken 22nd Jan 1996

Brahman cattle blood

Isolated in 1996

Shelf A15,

box A15 α,

rows A1-B10

 

(Added to collection 

 16th Oct 2008)

Virus ID: 96-13303-73

 

Tube labelled:

96-13303-73; IVD BHK/2; 3-12-08

E1/BHK2

 

*
Vial position A1 (g) from freezer A and B given to Kiki Nomikou (IAH-P) for sequencing studies (7th Oct 2010):
Corresponding vial from Freezer B was not removed.
*

Vial position B1(g) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013.  Corresponding vial in Freezer B has not been removed.

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

*

Reagent also available from:

USDA, Aphis, VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA

 

nvsluf@aphis.usda.gov

USA1994/05

BTV-10

 

typed by antibody disc neutralisation or virus neutralisation. 

 

Later confirmed by Seg-2 RT-PCR

Floyd County GA, USA

 

(sample taken

25th Jul 1994)

Virus isolated from Cattle blood

 

(animal described as "with ulcers and briefly off feed")

 

Isolation date unknown

Shelf A14,

box A14 β,

rows I1-J10

 

 (Added to collection  16th Oct 2008)

94-35171

 

 tube labelled

 94-35171;

YSD-1;

BHK-1

E2/BHK1

[+IAH/KC2]

 

related to USA1994/04

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section,

 Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

USA1994/04

BTV-10

 

typed by antibody disc neutralisation or virus neutralisation. 

 

Later confirmed by Seg-2 RT-PCR

Floyd County GA, USA

 

(sample taken

25th Jul 1994)

Virus isolated from Cattle blood

 

(animal described as "with ulcers and briefly off feed")

 

Isolation date unknown

Shelf D6  , 
Box D6 α,
Rows E1- F10
 
(added to 
collection 
11th May 2007)

94-35171

 

 tube labelled

 94-35171;

YSD-1;

BHK-1

E2/BHK1

[+IAH/BHK3]

 

related to

USA1994/05

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section,

 Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

USA1989/01

BTV-10

typed by antibody disc neutralisation

 

Later confirmed by Seg-2 RT-PCR

Marengo County AL

USA

 

(sample taken

5th Sep 1989)

Virus isolated from Cattle blood

 

17th Oct 1989

Shelf D5 , 
Box D5 γ,
Rows I1-J10 
 
(added to 
collection 
11th May 2007)

89-48705-125

 

 tube labelled

 89-48705-125YS;

BHK-1;

BTV ISOL;

10-17-89

E1/BHK1

[+IAH/BHK4]

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section,

 Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

USA1980/03

BTV-10

 

typed by antibody disc neutralisation or virus neutralisation. 

 

Later confirmed by Seg-2 RT-PCR

Yakima County WA

USA

 

(sample taken

23rd Sep 1980)

Virus isolated from Sheep blood

 

From an outbreak of Bluetongue disease  that occurred in Washington and Oregon States 1980

 

(Isolation date unknown)

Shelf A14,

box A14 γ,

rows G1-H10

 

(Added to collection 

 16th Oct 2008)

80-31040-6

 

 tube labelled

 80-31040;

6 VM/6;

BT10

E1/VERO6

 [+IAH/ KC2]

 

Related to sample

USA1980/02

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section,

 Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

USA1980/02

BTV-10

 

typed by antibody disc neutralisation or virus neutralisation. 

 

Later confirmed by Seg-2 RT-PCR

Yakima County WA

USA

 

(sample taken

23rd Sep 1980)

Virus isolated from Sheep blood

 

From an outbreak of Bluetongue disease  that occurred in Washington and Oregon States 1980

 

(Isolation date unknown)

Shelf D6  , 
Box D6 α
Rows C1-D10
 
(added to 
collection 
11th  May 2007)

80-31040-6

 

 tube labelled

 80-31040;

6 VM/6;

BT10

E1/VERO6

 [+IAH/ BHK 3]

 

Related to sample

USA1980/03

virus isolate supplied by 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section,

 Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

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