ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 9 (BTV-9)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection Contents

 

 

 

 

 Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus    (BTV) serotype 9

 *IAH dsRNA virus collection number

 

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 

 (identification method)

Place of original sample eg country town

 [grid reference]

 

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 

 Adapted to which cell types 

 

(Passage history of sample)

 

[IAH passage history]

* Original sample supplied to the collection by person / institution

 

[IAH, ISIS sample number]

Comments

 References &  accession numbers

 

  

Reference and vaccine strains

 RSArrrr/09

 

 

 

(41010EDA)

BTV - 9

 

(reference strain - 'western' topotype)

 

NK

Shelf B3,

box B3α

rows G1-H10

 

Added to collection on 9th May 2005

(Reference Strain) 

(E3/BHK6)

+

[IAH/BHK2]

Supplied by OVI S. Africa (recieved 22/12/82)

 

 

 

*

Duplicate samples  G1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

Vial H1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

Vial G3(g) A+B -  given to Kyriaki Nomikou  (IAH Pirbright) 2nd March 2010

*

Vial H2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

*

Vial H sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

*

[Seg-1: KP820968,

Seg-2: KP821088,

Seg-3: KP821210,

Seg-4: KP821330,

Seg-5: KP821450,

Seg-6:  KP821570,

Seg-7: KP821692,

Seg-8: KP821812,

Seg-9: KP821932,

Seg-10: KP822053,

Nomikou et al 2015 (4)]

 

 

 

  [S2:AJ585130, S3: DQ186795,

S6:AJ586708]

 

Maan et al 2007  contents

ITLvvv2/09

 

BTV 

Type 9

BTV-9 Vaccine

Strain

 

 

 

(data not available)

[BHK1]

Sample Derived from ITLvvv1/09, but passaged at IAH in BHK cells

 

ITLvvv1/09

 

BTV 

Type 9

BTV-9 Vaccine

 

produced in Italy

(IZS Terramo)

 batch number 006, expiry date date 06/04.

 

 Shelf D5,

box D5β,

rows I1-J10

 

Added to collection

 9th Feb 2007

BTV 9 vaccine

 

 batch number 006, expiry date date 06/04.

(data not available)

freeze dried monovalent commercial vaccine.  

(used to generate ITLvvv2/09)

  contents

  RSAvvv1/09

BTV 

Type 9

 

(Type confirmed by:

Seg 2 RT-PCR and  sequence analysis)

S.Africa

 

 14/04/02

NK 

Shelf A4,

Box A4β,

Rows A1-B10

Vaccine strain 

 

[R8/02, No 1]

BHK

 

(???)

[IAH- BHK2]

Sue Graham (June 2002)

 

[S5: AM778442]

  RSAvvv2/09 

BTV 

Type 9

 

(Type confirmed by:

Seg 2 RT-PCR and 

 sequence analysis)

S. Africa

Onderstepoort

Vaccine

Shelf A1,

Box A1α,

Rows C1-D10

 

Added to collection on

31st Jan 2002 

[BHK1]

Dr. Annalisa Guercio 

 IZS Palermo

Tubes C1 &D1 freezer A

 sent to  S. Anthony & H. Jones, 

Pirbright Institute 7th June 2004

 

  contents

[S2:AJ585173, S6:AJ586683

S8:AM900374

S9: EU220295]

RSAvvv3/09

BTV 

Type 9

S. Africa Onderstepoort Vaccine

 

Shelf B6, 
box B6γ,
rows E1-F10
 
Added to 
collection 
7th Sept 2005
Commercial 
Vaccine 
Batch no.2	
exp. 01/11/2005
 
BL6094S

(NK)

[IAH-BHK1]

Dr. Annalisa Guercio  IZS Palermo

 

 

 RSAvvv9/09

BTV

Type 9

S.Africa

 

 14/04/02

NK

 

Vaccine strain

 

[R8/02 No 9]

BHK

 

( )  

Sue Graham (June 2002)

 

 

 

 

2008

LIB2008/10

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR 

Libya

Blood  from Sheep with clinical signs of BT

 

(Field sample)

 

Shelf B10,

 box B10 b,

 rows E1-F10

 

Added to collection

 18th Sept 2009

Animal No C6

 [IAH/KC1/BHK1]

From :

Jimmy Elaref

 

Received on approximately  14th Oct 2008

 

[A160/08 17]

Isolate derived from LIB2008/07

 

LIB2008/09

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR

Libya

 

Blood  from Sheep with clinical signs of BT

 

(Field sample)

Shelf B10,

 box B10 b,

 rows C1-D10

 

Added to collection

 18th Sept 2009

Animal No C1

 [IAH/KC1/BHK1]

From :

Jimmy Elaref

 

Received on approximately  14th Oct 2008

[A160/08 12]

Isolate derived from LIB2008/04  
LIB2008/08

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR

Libya

 

Blood  from Sheep with clinical signs of BT

 

(Field sample)

Shelf B10,

 box B10 b,

 rows A1-B10

 

Added to collection

 18th Sept 2009 
Animal No b2  [IAH KC1/BHK1]

From :

Jimmy Elaref

 

Received on approximately  14th Oct 2008

[A160/08 5]

Isolate derived from LIB2008/01  

LIB2008/07

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR 

Libya

Blood  from Sheep with clinical signs of BT

 

(Field sample)

Shelf B7,

 box B7 γ,

 rows E1-F10

 

Added to collection

 23rd Jan 2009

Animal No C6

(IAH KC1 )

From :

Jimmy Elaref

 

Received on approximately  14th Oct 2008

 

[A160/08 17]

Used to Generate LIB2008/10

 

LIB2008/06

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR

Libya

 Blood  from Sheep with clinical signs of BT

 

(Field sample)

Shelf B7,

 box B7 γ,

 rows C1-D10

 

Added to collection

 23rd Jan 2009

 

Animal No C5

(IAH KC2 )

From:

 Jimmy Elaref

 

Received on approximately  14th Oct 2008

 

[A160/08 16]

 

 

LIB2008/05

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR

Libya

 

Blood  from Sheep with clinical signs of BT

 

(Field sample)

Shelf B7,

 box B7 γ,

 rows A1-B10

 

Added to collection

 23rd Jan 2009

Animal  No C3  (IAH KC1 )

From :

Jimmy Elaref

 

Received on approximately  14th Oct 2008

[A160/08 14]

   
LIB2008/04

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR

Libya

 

Blood  from Sheep with clinical signs of BT

 

(Field sample)

Shelf B7,

 box B7 β,

 rows I1-J10

 

Added to collection

 23rd Jan 2009

Animal No C1

[IAH/KC1]

From :

Jimmy Elaref

 

Received on approximately  14th Oct 2008

[A160/08 12]

Used to generate isolate LIB2008/09  
LIB2008/03

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR

Libya

 

Blood  from Sheep with clinical signs of BT

 

(Field sample)

Shelf B7,

 box B7 β,

 rows G1-H10

 

Added to collection

 23rd Jan 2009

Animal No Q4

 (IAH KC1 )

From :

Jimmy Elaref

 

Received on approximately  14th Oct 2008

[A160/08 9]

Vial position  G1 (g) from freezer A  given to Kyriaki Nomikou 24-Apr-2012 to prepare  RNA to be sent to Sanger  Centre for sequencing. 

LIB2008/02

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR

Libya

 

Blood  from Sheep with clinical signs of BT

 

(Field sample)

Shelf B7,

 box B7,

 β rows E1-F10

 

Added to collection

 23rd Jan 2009

Animal No Q3 (IAH KC1 )

From :

Jimmy Elaref

 

Received on approximately  14th Oct 2008

[A160/08 8]

Vial position  F10  (P) taken out of freezer A   and B on 29th Mar 2012
LIB2008/01

BTV-9

 

Identified by BTV group-specific

RT-PCR  [2]

 

Typed by

BTV-9 specific real-time PCR

Libya

 

Blood  from Sheep with clinical signs of BT

 

(Field sample)

Shelf B7,

 box B7 β,

 rows C1-D10

 

Added to collection

 23rd Jan 2009

Animal No b2  (IAH KC1 )

From :

Jimmy Elaref

 

Received on approximately  14th Oct 2008

[A160/08 5]

Used to generate LIB2008/08
*

Vial positions  E1 (p)  were given to Eva Veronesi (IAH Pirbright on 13th May 2010 for Ph.D studies).  Spare vial retained in freezer B position E1.  

*

Vial D10 freezer A given to C. Batten  (IAH-P) 9th June 2009

Spare vial retained in freezer B position D10

*

Duplicate vials position C2g , taken out of freezers A and B on 6th Sept 2010

*

Vial position  D2  (P) taken out of freezer A   and B on 29th Mar 2012

2005
IND2005/08 BTV-9 Southern India from
sheep

Sample taken in 2005

Shelf ??

box ??,

rows ??

 

(added to collection

2006)

unknown passage level in BHK cells

[IAH/KC1/BHK1]
Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies
*
Derived from IND2005/07

(MTA)
IND2005/07 BTV-9 Southern India from
sheep

Sample taken in 2005

Shelf ??

box ??,

rows ??

 

(added to collection

2006)

unknown passage level in BHK cells

[IAH/KC1]
Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies
*
Used to generate IND2005/08

(MTA)

IND2005/03

BTV-9

Hyderabad,

India 

 

Shelf D4

box D4 β,

rows A1-B10

 

(added to collection

 21st Nov2006)

[+IAH/ BHK3]

 

Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies

(MTA)
IND2005/02 BTV-9

Hyderabad,

India

 

Shelf D4

box D4α,

rows I1-J10

 

(added to collection

 21st Nov2006)

 
 [+IAH/ BHK3]   Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies [S8: AM900385]

(MTA)

IND2005/01

BTV-9

Hyderabad,

India

Heparinised Blood

Shelf D4,

box D4α,

rows G1-H10

 

(added to collection

 21st Nov2006)

 [+IAH/ BHK3]

 

Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies

(MTA)
MAR2005/06

BTV-9

Martinique (France)

(Sample taken
Dec  2005)
from a
non-vaccinated bovine
Shelf C7,
box C7 α ,
 rows A1-B10  

(Added to collection
 9th Aug 2010)

tube labelled:

W1B5 xx  130

E1BHK5

[IAH/BHK1 ]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France

23rd July 2010
Distribution restricted by MTA

[S2:   ]
2004

IND2004/04

BTV-9

Hyderabad,

India

Heparinised Blood

Shelf D4,

box D4α,

rows E1-F10

 

(added to collection on  20th Nov 2006) 

 

 [IAH/ BHK3 ]

 

Sample given to Sushila Maan IAH Pirbright Sept 2006 for sequencing studies

(MTA)

IND2004/02

BTV-9

Andhra Pradesh

 

Sample taken

 2004

From Blood

Shelf D3

box D3γ,

rows I1-J10

 

(added to collection 

7th Nov 2006)

No. 12

[IAH/BHK3]

 

Sample given to Sushila maan IAH Pirbright Sept 2006 for sequencing studies

(MTA)
2003

IND2003/11

BTV-9

India

 

Shelf D5

Box D5 ALPHA

Rows C1-D10

 

(added to collection on

27th Nov 2006)

 [IAH/ BHK3 ]

 

Sample given to Sushila maan IAH Pirbright Sept 2006 for sequencing studies

27th Nov 2006

(MTA)

 ITL2003/01   

BTV

 type 9

 

(Type confirmed by:

Seg 2 RT-PCR

and  sequence analysis)

Sicily Farm code: 33306

Province: Nissoria (Enna)

 

 (2nd Apr.2003)

Sheep (04/11/2003)

Shelf A3,

Box A3γ,

Rows I1-J10

 

(5326/2003)

from spleen

 

( BHK4)

[IAH-BHK3]

Dr. Annalisa Guercio &

Giuseppina Alimena

IZS Palermo

 From Sheep spleen

died six days after vaccination

Vial  I9 (freezer A & B) given to Kyriaki Nomikou 

(Pirbright) on 27th Jan 2010

 *

  Tubes I1and I10 were removed from the collection

 *

2002

BOS2002/04

BTV Type 9

 

(Identified as BTV by:  ELISA.

Serotype identified by SNT)

 Village: Milici Town: Vlasenica State/Province: Republike Srpska Country: Bosnia Herzegovina

 (13-14/8/02)  

sheep

 

(IAH Pirbright 07/09/2002 Sue Graham)  

Shelf C2,

Box C2β,

Rows I1-J10

 

Added to collection on

25th Oct 2005 

(Bos 890)

[ R13-890] 

 BHK

From blood

 

( E1/BHK1)

 

[IAH/BHK2]

 Dr Violeta Santrac Veterinary Institute,"Dr Vaso Butozan", Banja Luka

Grown in BHK cells, then stored at -70 C

 

[S10:   ]

 BOS2002/03

BTV Type 9

 

(Identified as BTV by:  ELISA.

Serotype identified by SNT ) 

 Village: Milici Town: Vlasenica State/Province: Republike Srpska Country: Bosnia Herzegovina (13-14/8/02) 

Isolated from sheep

 

(Isolate made IAH Pirbright 7th Sept 2002 by Sue Graham)  

Shelf C4, 
box C4γ
rows E1 - F10
 
(added to 
collection
  8th Dec 2005)

 

(877)

 

[ R13-877] 

Adapted to BHK cells

 

Isolated from blood 

 

E1/BHK1

[IAH/BHK2]

Dr  Violeta Santrac

 

 Veterinary Institute, "Dr Vaso Butozan", Banja Luka

Grown in BHK cells, then stored at -70 C  

 

[S10:   ]

 BOS2002/02

BTVType 9

 

(Identified as BTV by:  ELISA.

Serotype identified by SNT and confirmed by Seg-2 RT-PCR and sequence analysis)

 Village: Milici Town: Vlasenica State/Province: Republike Srpska Country: Bosnia Herzegovina

 

 (13-14/8/02) 

sheep

 

(IAH Pirbright 03/09/2002 by Sue Graham)

Shelf B5,

Box B5β,

Rows A1-B10

 

Added to collection on 1st Aug 2005

 

876)

 

[ R13-876]  

BHK

From blood

 

 (E1/BHK1)

[IAH/BHK2]

 (  Dr Violeta Santrac Veterinary Institute,"Dr Vaso Butozan", Banja Luka

*

Grown in BHK cells, then stored at -70 C

*

[ S6:AJ586687, S8: AM900377,

S9: EU220294]

*

Vial positions  E1 (p)  were given to Eva Veronesi (IAH Pirbright on 13th May 2010 for Ph.D studies).  one of these was retained in Misc box C,  bottom of freezer A
position E3

*

BOS2002/01

BTV Type 9

 

(Identified as BTV by:  ELISA

Serotype identified by SNT)

Village: Milici Town: Vlasenica State/Province: Republike Srpska Country: Bosnia Herzegovina

 

 (Sample taken 13-14/8/02)

isolated from sheep

 

 (isolate made

9th Sept 2002

 at IAH Pirbright by Sue Graham)

 

Shelf C4, 
box C4γ
rows C1 - D10
 
(added to 
collection
  8th Dec 2005)

(Bos 875) 

 

[ R13-875]

Adapted to BHK cells

 

Isolated from blood 

 

E1/BHK1

[IAH/BHK2]

 Dr Violeta Santrac Veterinary Institute,"Dr Vaso Butozan", Banja Luka

 

[S5: AM778443]

2001

KOS2001/04

BTV-9

 

(Identified as BTV by:  ELISA

Serotype identified

by SNT)

Kosovo

 

2001

Sheep

 

(isolated by Sue Graham at IAH Pirbright)

Shelf A6,

BoxA6α,

Rows C1-D10

 

From blood sample

 [IAH/E1/BHK1]

Philip Deng

&

Dr Artan Osmani

 

[R17/01]

Tube C1 (freezer B)  given to Eva Veronesi (Pirbright)  27th Sept 2005

Tube D1  (freezer A and B) given to Kyriaki Nomikou (Pirbright) 20th Jan 2010

Tube D2 was removed from freezer B

*

Tube D3 was removed from freezer A&B

*

KOS2001/03

BTV Type 9

 

(Identified as BTV by:  ELISA

Serotype identified

by SNT)

municipality of Podujevo (30km north of Pristina), village of Sllatina.

Samples collected on 30/08/2001

Sheep

 

(isolated by Sue Graham at IAH Pirbright)

Shelf A6,

BoxA6α,

Rows I1-J10

No.34688

From blood sample

 [IAH/E1/BHK4]

Philip Deng

&

Dr Artan Osmani

 

[R17/01]

Vial I1 (freezer  A and B) given to Kyriaki Nomikou (Pirbright)

20th Jan 2010

KOS2001/02

BTV Type 9

 

(Identified as BTV by:  ELISA

Serotype identified

by SNT)

Municipality of Glogovac (35km west of Pristina), village of Sankovc, Kosovo

Samples collected on 12/09/2001

Sheep

 

(isolated by Sue Graham at IAH Pirbright)

Shelf A5,

Box A5γ,

Rows  C1-D10

No.34681

From blood sample

 [IAH/E1/BHK1]

 

Philip Deng

&

Dr Artan Osmani

 

[R17/01]

 

 

KOS2001/01

BTV Type 9

 

(Identified as BTV by:  ELISA

Serotype identified

by SNT)

municipality of Viti  (35km southeast of Pristina), village of Sadovine.

Sample collected on 12/09/2001

Sheep

 

(isolated by Sue Graham at IAH Pirbright)

Shelf A6,

BoxA6α,

Rows G1-H10

No.06244

From blood sample

 [IAH/E1/BHK2]

Philip Deng

&

Dr Artan Osmani

 

[R17/01]


*
Vial position G1 from freezer A and B given to Kiki Nomikou (Pirbright) for sequencing studies (7th Oct 2010).
*

 SER2001/02

BTV Type 9

 

(Identified as BTV by: ELISA)

 

Serbia

(07/10/01)

sheep   

Shelf A1,

Box A1γ,

Rows I1-J10

 [R17/34678]

E1/BHK2  

 

 

[ ]

 SER2001/01

BTV Type 9

 

(Identified as BTV by: ELISA.   Type confirmed by:

Seg 2 RT-PCR

and sequence analyses )

Serbia

( 07/10/01 )

sheep    

Shelf B5,

Box B5β,

Rows E1 - F10

 

 added to collection on 1st Aug 2005

[R17/06547]

E1/BHK2  

 

[IAH/BHK2]

 

 

 [ S6:AJ586688]

TUR2001/03

BTV-9

Identified by

 BTV group-specific

Real time RT-PCR  [2]

Typed  by conventional RT-PCR [1] and type specific Real-Time PCR

 

 

Turkey


Sample collected in 2001

From sheep blood

Shelf B14,

box B14 γ ,

rows I1-J10

 

Added to collection

25th Jan 2010

Iso15

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/BHK1]

 (c)
TUR2001/02

BTV-9

Identified by

 BTV group-specific

Real time RT-PCR  [2]

Typed  by conventional RT-PCR [1] and type specific Real-Time PCR

Turkey


Sample collected in 2001

From sheep blood

Shelf B14,

box B14 γ ,

rows G1-H10

 

Added to collection

25th Jan 2010

Iso14

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/ BHK1]

 (c)
TUR2001/01

BTV-9

Identified by

 BTV group-specific

Real time RT-PCR  [2]

Typed  by conventional RT-PCR [1] and type specific Real-Time PCR

 

 Turkey


Sample collected in  2001

From sheep blood

Shelf B14,

box B14 γ ,

rows E1-F10

 

Added to collection

25th Jan 2010

Iso13

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/BHK1]

 (c)
2000

TUR2000/12

BTV Type 9

(Type confirmed by:

Seg 2 RT-PCR)

Turkey

 

(year 2000)

Shelf A4,

Box A4α

 Positions

G1-H10

BTTR 2000/12

 [R13/00 no 12]

[IAH-BHK2]

Dr. Nahit Yazicioglu,

Director , Ministry of agriculture and rural affairs,

Etlik veterinary control and research institute

06020 ETLIK-Ankara/Turkey

 6th Oct 2000

contents

[S10:     ]

TUR2000/11

BTV Type 9

(Type confirmed by:

Seg 2 RT-PCR)

Turkey

(year 2000)

Shelf A4,

Box A4α

rows E1-F10

BTTR 2000/11

[R13/00 no 11]

[IAH-BHK2]

Dr. Nahit Yazicioglu,

 Director , Ministry of agriculture and rural affairs,

 Etlik veterinary control and research institute

06020 ETLIK-Ankara/Turkey

 6th Oct 2000

[S10:    ]   

TUR2000/05

BTV Type 9

 

 

(Type confirmed by:

Seg 2 RT-PCR

and sequence analyses )

Manisa,

Turkey

 

 (year 2000)  

 

Shelf B1,

box, B1γ,

rows I1-J10

 

February 2005

 

BTTR 2000/5

 

[R13/00 no 5]

(E3/BHK6)

 

[IAH/BHK1]

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey

 6th Oct 2000

Sample Nos. 1/10g(a+b)  were given to Natalie Ross-Smith (IAH Pirbright) for sequencing studies,

 7th March 2006

contents

 

 

 

 

[S6:AJ586686]

TUR2000/04

BTVType 9

 

(Type confirmed by:

Seg 2 RT-PCR

and sequence analyses )

 

 Denizli, Turkey

 (year 2000)

Shelf C3, 
box C3α,
rows I1 - J10
 
(added to 
collection on 
15th Nov 2006)

(BTTR 2000/4)

 

[R13/00 no 4]

(E3/BHK7)

 

[IAH/BHK2]

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey

 6th Oct 2000

contents

 

 

[S6:AJ586685 ]

 TUR2000/03

BTV Type 9

 

(Identified as BTV by: ELISA.   Type confirmed by:

Seg 2 RT-PCR

and sequence analyses )

 

Turkey  MANISA

 

(year 2000) 

 

Shelf C3, 
box C3β,

rows G1 -H10

 

(added to collection on 18th Nov 2005)

BTTR 2000/3

 [R13/00 No 3]   

E2/BHK7

[IAH/BHK2]

Dr. Nahit Yazicioglu, Director , Ministry of agriculture and rural affairs, Etlik veterinary control and research institute 06020 ETLIK-Ankara/Turkey

 6th Oct 2000

 

 [S2: AJ585175,

S5:AM778445,

S6:AJ586684

S8: AM900376]

 GRE2000/02

BTV Type 9

 

(Identified as BTV by: ELISA.   Type confirmed by:

Seg 2 RT-PCR

and sequence analyses )

Greece (22/02/00)

Sheep       

Shelf B5,

Box B5β,

Rows G1 - H10

 

 added to collection on 1st Aug 2005

 

 (408/99)

E2/BHK4 

 

[IAH/BHK1]

 

 

[S2:

S10:    ]

1999
TUR1999/06

BTV-9

Identified by BTV group-specific

Real time RT-PCR  [2]

Typed  by conventional RT-PCR [1] and type specific Real-Time PCR

 Turkey


Sample collected in  1999

From sheep blood

Shelf B15,

box B15 β ,

rows A1-B10

 

Added to collection

29th Jan 2010

Iso11

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/ ]

 (c)

TUR1999/05

BTV-9

Identified by BTV group-specific

Real time RT-PCR  [2]

 

Typed  by conventional RT-PCR [1] and type specific Real-Time PCR

 

Turkey


Sample collected in 1999

From sheep blood

Shelf B15,

box B15 α ,

rows E1-F10

 

Added to collection

25th Jan 2010

Iso9

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/ BHK1]

 (c)

TUR1999/04

BTV-9

Identified by BTV group-specific

Real time RT-PCR  [2]

Typed  by

conventional RT-PCR [1] and type specific Real-Time PCR

Turkey


Sample collected in  1999

From sheep blood

Shelf B15,

box B15 α ,

rows I1-J10

 

Added to collection

29th Jan 2010

Iso8

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/ ]

 (c)

TUR1999/03

BTV Type 9

Identified as BTV type 9

by RT-PCR

Turkey


Sample collected in June 1999

From sheep blood

Shelf    D17

Box   D17ɑ

Rows D1-D9

 

(Added to the

collection on

21st Nov 2014)

 

&

 

Shelf B15,

boxB15 Beta,

rows I1-J10 

 

Iso7

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/BHK1 ]

 (c)

 

Only 9 vials stored in each freezer

TUR1999/02

BTV-9

Identified by BTV group-specific

real time RT-PCR  [2]

 

Typed  by  conventional RT-PCR [1] and type specific Real-Time PCR

Turkey


Sample collected in 1999

From sheep blood

Shelf B14,

box B14 γ ,

rows C1-D10

 

Added to collection

25th Jan 2010

Iso4

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/ BHK1]

 (c)

TUR1999/01

BTV Type 9

Identified as BTV type 9

by RT-PCR

 

Turkey


Sample collected in

August 1999

from sheep blood

Shelf   D17

Box   D17ɑ

Rows C1-C10

 

(Added to the

collection on

21st Nov 2014)

 

&

 

Shelf B15,

boxB15 Beta,

rows C1-D10 

 

Iso1

From Blood adapted to Vero cells


ECE/BHK/Vero

[IAH/ BHK1]

 (c)

GRE1999/32

BTV9 Identified by BTV group-specific Seg-7 RT-PCR and Real time RT-PCR  [2].
Typed by

SNT,

convetional RT-PCR [1] and specific real-time PCR 

GREECE:

   Lesbos,

Aghia Paraskevi  (Central island)   

39 25' N

26  27'  E

( sample collected on
 25th Nov 1999)

isolated from

cattle spleen,

 

at IAH Pirbright

by: Nomikou K

Shelf C8,

Box C8α

Rows I1-J10

 

(added to

collection

25/08/2010

GR 732a/99LB

From cattle spleen

[IAH KC1/BSR1] 

Derived from same animal as  isolate GRE1999/30
and GRE1999/31

Animal in the same herd as  isolate
 GRE1999/29

GRE1999/31

BTV9

Identified by BTV group-specific Seg-7 RT-PCR and

Real time RT-PCR  [2]

Typed  by SNT,

convetional RT-PCR [1] and specific real-time PCR 

GREECE:

   Lesbos,

Aghia Paraskevi  (Central island)   

 39 25' N

26  27'  E

 

( sample collected on
 25th Nov 1999)
isolated from 
cattle spleen, 
 
at IAH Pirbright
by: Nomikou K.

Shelf B14,

box B14β ,

rows I1-J10

 

Added to collection

20th Jan 2010

GR 732a/99LB

From cattle spleen

 

[IAH KC1] 

 

  Derived from same animal as  isolate GRE1999/30
Used to generate
GRE1999/32

Animal in the same herd as  isolate
 GRE1999/29
GRE1999/30

BTV9

Identified by BTV group-specific Seg-7 RT-PCR and

Real time RT-PCR  [2]

Typed  by SNT,

convetional RT-PCR [1]and specific real-time PCR 

GREECE:

   Lesbos,

Aghia Paraskevi  (Central island)   

 39 25' N

26  27'  E

 

( sample collected on
 25th Nov 1999)
isolated from 
cattle 
lymphnode 
 
at IAH Pirbright
by: Nomikou K.

Shelf B14,

box B14β ,

rows G1-H10

 

Added to collection

20th Jan 2010

GR 732a/99LB

From cattle lymphnode

 

[IAH KC2] 

  Derived from same animal as  isolate GRE1999/31

Animal in the same herd as  isolate
 GRE1999/29
 

GRE1999/12

BTV Type 9

 

Identified as BTV by ELISA / RT-PCR,

&

as type 9 by neutralisation assay

Ierissos,

Chalkidiki,

Greece

(N. Central)

 

[40 24΄Ν,

23 52΄Ε]

 

29th Sept. 1999

Sheep (blood)

 

At : Centre of Athens Vet. Institutions, Virus Lab, Athens.

By: K.Nomikou &

O. Mangana

Shelf B1,

box B1γ,

rows A1-B10

 

Added to collection on 30th Sept 2004

GR408/99ChS

BHK 21 CELLS

 

from blood

 

(E2BHK4)

 

[IAH/BHK2]

Dr. Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.

Seg-10 sequenced at E2/BHK7

[Seg3:DQ186805

Seg10:AY449651]

GRE1999/06

BTV Type 9

Chalkidiki,

Greece

 

[40 24΄Ν,

23 52΄Ε]

 

 (1999)

 Sheep 

Shelf A3,

box A3α,

 rows I1-J10

 

(January 2003)

(GR-408/99ChS)

From blood

 

[IAH-BHK4]

 

Dr. Charalabos Billinis Aristotle University Thessalonik

Tubes I1 and J1 (freezer A&B)  given to Eva Veronesi

 (Pirbright) on  27th Sept 2005

*

Tubes J2 (freezer A&B) removed from collection)

Tubes J3 (freezer A&B) given to T.Jabbar (Pirbright)

 on 25th Jan 2008

*

[S10:   ]

BUL1999/01

BTV Type 9

 

(Identified as BTV by: ELISA.  

Type confirmed by:

Seg 2 RT-PCR

and sequence analyses )

Bulgaria

 

 (29/09/99)    

NK

Shelf A4,

Box A4γ,

Rows C1-D10

(TR 99-1; V8/99)

(E1/BHK3)

[IAH-BHK2]

 

 

 [S3:  DQ186797

S5: AM778444]

1998

GRE1998/01

 

 BTV-9

 

Identified as BTV by ELISA and  RT-PCR,

as type 9 by serum neutralisation

Afandou,

Rhodes,

Greece

(SE)

 

[36 17΄Ν,

28 08΄ Ε]

 

(3rd Nov 1998)

 

sheep  (blood

At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana

 

Shelf B2,

Box B2α,

Rows A1-B10

 

added to collection on

30th Sept.2004

GR199/7a/98RS

 

(Animal code No.  7a)

BHK 21 cells

 

 BLOOD

 

 (E2BHK9)

[IAH-BHK1]

Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab,

Athens.

Seg-10 sequenced at E2/BHK11

 

[S3:DQ186808

S10:AY677629]

 

Vials position A1 given to K. Nomikou (IAH-P) for sequencing 22nd July 2009

 

Vial position  A2 (g) from freezer A  given to Kyriaki Nomikou 24-APR-2012 to prepare  RNA to be sent to Sanger  Centre for sequencing.  The corresponding tube was not removed from freezer B

TUR1998/06

BTV-9

Identified by

 BTV group-specific

Real time RT-PCR  [2]

Typed  by conventional RT-PCR [1] and type specific Real-Time PCR

Turkey


Sample collected in 1998

from sheep blood

Shelf B15,

box B15 α ,

rows A1-B10

 

Added to collection

25th Jan 2010

Iso12

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/ BHK1]

 (c)

TUR1998/05

BTV-9

Identified by

 BTV group-specific

Real time RT-PCR  [2]

Typed  by conventional RT-PCR [1] and type specific Real-Time PCR

Turkey


Sample collected in 1998

from sheep blood

Shelf B15,

box B15 α ,

rows C1-D10

 

Added to collection

25th Jan 2010

Iso10

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/BHK1]

 (c)

TUR1998/04

BTV Type 9

Identified as BTV type 9

by RT-PCR

Turkey


Sample collected in September 1998

from sheep blood

Shelf    D17

Box   D17ɑ

Rows B1-B10

 

(Added to the

collection on

21st Nov 2014)

 

&

 

Shelf B15,

boxB15 Beta,

rows G1-H10 

 

Iso6

From Blood adapted to Vero cells


ECE/BHK/Vero

[IAH/BHK1 ]

 (c)

TUR1998/03

BTV Type 9

Identified as BTV type 9

by RT-PCR

Turkey

 

Sample collected in 1998

from sheep blood

Shelf B15,

boxB15 Beta,

rows E1-F10 

Iso5

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/ ]

 (c)

TUR1998/02

BTV-9

Identified by BTV group-specific

Real time RT-PCR  [2]

Typed  by

conventional RT-PCR [1] and type specific Real-Time PCR

Turkey

Sample collected in 1998

from sheep blood

Shelf B15,

box B15 α ,

rows G1-H10

 

Added to collection

29th Jan 2010

Iso3

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/ ]

 (c)

TUR1998/01

BTV-9

Identified by BTV group-specific

real time RT-PCR  [2]

 

Typed  by  conventional RT-PCR [1] and type specific Real-Time PCR

Turkey


Sample collected in 1998

from sheep blood

Shelf B14,

box B14 γ ,

rows A1-B10

Added to collection

25th Jan 2010

Iso2

From Blood adapted to Vero cells


ECE/BHK/Vero

 

[IAH/ BHK1]

 (c)

1991
ISA1991/09 BTV-9 Indonesia

10/07/1991
  Shelf D13, Box D13γ,
row J1 to J10

(Added to

collection on 2nd

October 2014)


&
Shelf D16,
Box D16 α,
rows B1 to B10
KN (D15)

D15
[BHK3]    
1988

AUS1988/02

BTV-9

Australia

(1988)

 

Old isolate No.: Y920  

 

Origin: DPP837

 

Sample sent as 'BTV-9'

??/2BHK21 (PP)

[IAH/??]

Peter Kirkland,

Elizabeth Macarthur Agricultural Institute, Menangle, NSW 2568 Australia

Sent: 7th Sept 2010

Note  supplied with  sample: CPE positive / BT-PCR positive

 

It is uncertain if the date given is sampling date or date of arrival at EMAI.

 

ex BARC

 

(MTA)

1985
AUS1985/01

BTV-9

Australia,

 Northern Territories

 

 

1985

(G. Gard)

Shelf A6

Box A6β

Rows G1-H10

(DPP836,

8805-25-1105)

E1/BHK3

[IAH-BHK1]

Dr. Ian Pritchard

AAHL Geelong,

Australia

*MTA* , not to  be supplied   to 
3rd parties without written 
consent from original source
 

[Seg3:DQ186790]

 

Aliquots H1 (freezer a & b)) given to Eva Veronesi (Pirbright)  27th Sept 2005

 * 

Vial H3 (freezer A&B)  given to Kyriaki Nomikou (Pirbright)  20th Jan 2010

*

Vial position H2 (freezer A)  given to Kyriaki Nomikou 24-Apr-2012 to prepare  RNA for sequencing at the Sanger  Centre  

*

 Vial H4 was removed from freezer A

*

  Vial G1 was removed from freezer B.

1971

RSA1971/02

BTV-9

 

Identified by serology

Grown at IAH in Dorset Poll sheep from a sample of virus  originally isolated between

1968-1972

 

 

(3080)

no passage in cell culture

This virus has been passed 4 times in sheep

It has been grown in Eggs and the last two sheep passes were infected with freeze dried blood plus infected egg material  

Freeze dried Virus in sheep blood

(Dorset Poll)

 

Unknown isolation date
TUR----/04

BTV 9

Identified by seg-2 specific RT-PCR
Turkey Isolated in Turkey

Shelf A17,

box A17 β,

rows G1H10

  [IAH BHK2]

Supplied by Piet Van Rijn CIDC

Lelystad

 

[Stars No.  A140/08 no 63]
Although the sample came via Iran, it is believed that it originated from Turkey  

TUR----/01

BTV-9

Iran

(isolated in Iran)

 

A140/08 number 63

KC cells

 

[IAH/BHK1]

supplied by Dr. Mahmod Azimi

 

Razi Vaccine & Serum Research Institute, Karaj, Iran P.O.BOX:31975/148

 

 

*MTA* , not to be supplied  to 3rd parties without written consent from original source
 
References
1:

Mertens PP, Maan NS, Prasad G, Samuel AR, Shaw AE, Potgieter AC, Anthony SJ, Maan S. (2007) Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains. J Gen Virol. 88, 2811-2823.

2:

Shaw AE, Monaghan P, Alpar HO, Anthony S, Darpel KE, Batten CA, Guercio A, Alimena G, Vitale M, Bankowska K, Carpenter S, Jones H, Oura CA, King DP, Elliott H, Mellor PS, Mertens PP. (2007) Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1. J Virol Methods. 2007 Nov;145(2):115-26. Epub 2007 Jun 21.

3:

Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PP. (2007) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes.J. Gen Virol. 2007 Feb;88(Pt 2):621-30.

4:

Nomikou K, Hughes J, Wash R, Kellam P, Breard E, Zientara S, Palmarini M, Biek R, Mertens P. (2015) Widespread Reassortment Shapes the Evolution and Epidemiology of Bluetongue Virus following European Invasion. PLoS Pathog. 2015 Aug 7;11(8):e1005056. doi: 10.1371/journal.ppat.1005056. eCollection 2015.

 
 

 

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