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Isolates of
Bluetongue virus type 22
(BTV-22)
in the dsRNA virus collection at IAH Pirbright
Return to Virus Collection »
Contents
|
Family:
Reoviridae, Genus: Orbivirus, Species:
Bluetongue virus
serotype 22
(BTV-22) |
|
Virus Species
(identification method) |
Serotype
(identification method) |
Place of original sample
eg country town
[grid reference]
(date original sample was taken)
|
Isolated from which host species
e.g cow, sheep, etc
(Date of virus isolation, where, by whom)
|
Position of storage in reference freezers
(Date added to collection)
|
IAH dsRNA virus collection number: [Country code year / sample number]
(Suppliers Isolate Designation) |
Adapted to which cell types
Original sample material
(Passage history of sample) |
* Original sample supplied to the collection by
person / institution
[IAH, ISIS sample number] |
Comments,
References
&
accession numbers |
|
RSArrrr/22
|
BTV-22
(western topotype reference strain) |
Supplied by OVI
S. Africa*
(09/10/87) |
NK |
Shelf B6,
box B6γ,
rows C1-D10
Added to
collection
7th Sept 2005
|
(Ref. Strain)
|
Adapted to BHK cells
E2/BHK1/
CER1/BHK3
[IAH-BHK1] |
*
Duplicate samples
C1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co.,
Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330,
Korea
*
Vial D1 (p)
A&B sent to Mehdi El Harrak, Biopharma , Morocco 29th June 2009
*
Vial D2 sent to
Denis Kolbasov, Russian National
Institute of Veterinary Virology 29th Sept 2009
*
Vial C sent to Dr Graham Abdeljelil,
Institut Pasteur de Tunis 8th
March 2011
*
|
[Seg-2:
AJ585143,
Seg-6:
AJ586725,
Maan et al 2004
(1),
Singh et al 2004
(3),
Maan et al 2007
(2)]
» contents
|
|
MAR2005/05 |
BTV-22 |
Martinique (France)
(Sample taken Dec 2005) |
from a
non-vaccinated bovine |
Shelf C8,
Box C8α
Rows G1-H10
(added to collection 25/08/2010) |
tube
labelled:
BTV-22/06.01 - 21 |
[IAH/BHK1 ] |
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France
23rd July 2010 |
Distribution restricted by MTA
[S2: ] |
|
USA2002/02 |
BTV-22
(serotyped
by Seg-2
RT-PCR
and
serquence
analysis) |
Okeechobee County FL, USA
(sample taken
30th Oct 2002)
|
From cattle
Sample submitted for export
testing, no clinical history given
(isolate preparation date 7th Aug
2006) |
Shelf E1,
Box E1 alpha,
rows I1 - J10
(added to
collection on 15th June 2007) |
03-220082-5
(tube labelled: 220082-5;
IVD BHK/1;
8-7-06)
|
From blood
E1/BHK1/E1/BHK1
[IAH/ BHK4] |
virus isolate supplied by :
USDA, Aphis, VS, NVSL, PAS Reagents Office.
PO box 844 Ames .IA 50010
USA
nvsluf@aphis.usda.gov
Technical contact in USA: Donna
Johnson
Equine and Ovine Viruses Section,
Diagnostic virus Lab, PO box 844
Ames IA, 50010 USA.
repeated passage of original isolate
generated virus of low titre,
so
the original isolate was inoculated
into ECE in July 2006,
followed by
one pass in BHK-21 cells.
original typing attempts
as one of previous US
types
were unsuccessful. Multiple other serotypes were
isolated from this herd (BTV-3, 10
and two isolates of unknown serotype
- see USA2002/03).
|
[Seg-2: ]
|
|
TAT1990/01 |
Initial attempts
to identify serotype by SNT were unsuccessful in 1990
However, type
specific RT-PCR and sequencing (Seg-2) March 2008 identified
type 22
[1] |
Trinidad:
St. Andrews
Sample taken
4th Sept 1990 |
Water buffalo
blood
Isolated in
1990 |
Shelf A14,
box A14 γ,
rows I1-J10
(Added to collection
16th Oct 2008) |
Virus ID:
90-44159-70
Tube labelled:
90-44159-70;
YSD BHK/5 - CPAE/1 - BHK/2;
3-12-08 |
E1/BHK5/
CPAE1/BHK2 |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
Reagent also
available from: USDA, Aphis,
VS, NVSL, PAS, Attn:
Reagents Office P.O. Box 844 Ames, IA 50010 USA
nvsluf@aphis.usda.gov
|
|
|
NIG1982/11 |
BTV
Type 22 |
Nigeria
(08/08/82) |
NK |
(Added to collection on
21st Dec 2005) |
(N-421)
|
Adapted to BHK cells
E1/BHK3
[IAH/BHK2] |
|
[Seg-2:
Seg-6:
AJ586726]
|
NIG1983/01
|
BTV
Type 22
|
Nigeria
(01/05/83)
|
NK |
Shelf C4,
box C4α
rows E1-F10
(Added to
collection on
21st Dec 2005)
|
(N-415)
|
Adapted to
BHK cells
E1/BHK11
[IAH/BHK2]
|
|
|
|
NIG1982/12 |
BTV
Type 22 |
Nigeria
(13/08/82) |
NK |
Shelf C4,
box C4α
rows I1-J10
(Added to collection on
21st Dec 2005) |
N-441
|
Adapted to BHK cells
E1/BHK4
[IAHBHK2] |
|
|
*IAH dsRNA virus collection number:
[Country code, year / sample number] rrrr = reference strain vvvv =
vaccine strain]
References |
| 1.
|
Maan S, Maan NS, Samuel AR, O'Hara R, Meyer AJ, Rao S, Mertens PP.
(2004) Completion of the sequence analysis and comparisons of genome
segment 2 (encoding outer capsid protein VP2) from representative
isolates of the 24 bluetongue virus serotypes. Vet Ital. 2004
Oct-Dec;40(4):484-8.
http://www.ncbi.nlm.nih.gov/pubmed/20422574
|
| 2. |
Maan S, Maan NS, Samuel AR, Rao S, Attoui
H, Mertens PPC. (2007) Analysis and phylogenetic comparisons of
full-length VP2 genes of the 24 bluetongue virus serotypes. Journal
of General Virology 88, 621-630.
|
| 3. |
Singh KP, Maan S, Samuel AR, Rao S, Meyer AJ, Mertens PP. (2004)
Phylogenetic analysis of bluetongue virus genome segment 6 (encoding
VP5) from different serotypes. Vet Ital. 2004 Oct-Dec;40(4):479-83.
http://www.ncbi.nlm.nih.gov/pubmed/20422573
|
| 4. |
St George TD, Standfast HA, Cybinski DH, Dyce AL, Muller MJ, Doherty
RL, Carley JG, Filippich C, Frazier CL. (1978) The isolation of a
bluetongue virus from Culicoides collected in the Northern Territory
of Australia. Aust Vet J. 1978 Mar;54(3):153-4.
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