Isolates of Bluetongue virus type 1 (BTV-1)
in the dsRNA virus collection at IAH Pirbright
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|
Reference and vaccine strains |
2014 | 2012 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2001 | 1999 | 1996 | 1997 | 1992 | 1988 | 1987 | 1985 | 1983 | 1982 | 1981 | 1979 | Unknown sampling date |
| Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus, serotype 1 (BTV-1) | ||||||||||
|
*IAH
dsRNA virus collection number
|
Virus Species & Serotype
(identification method) |
Place of original sample eg country town [grid reference]
(date original sample was taken) |
Isolated from which host species e.g cow, sheep, etc
(Date of virus isolation, where, by whom) |
Position of storage in reference freezers
(Date added to collection) |
Suppliers Isolate Designation |
Original sample material,
Adapted to which cell types
(Passage history of sample)
[IAH passage history] |
* Original sample supplied to the collection by person / institution
[IAH, ISIS sample number] |
Comments References & accession numbers |
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| Reference and vaccine strains | ||||||||||
|
(41010B4F) |
BTV Type 1 (Type confirmed by Seg-2 RT-PCR and sequencing)
For reference strain of BTV-1 'Eastern topotype' - BTV-1(e), see IND1992/02 |
Supplied by OVI S. Africa* |
NK |
Shelf B2, boxB2β, rows E1-F10
(added to collection on 17th March 1982) |
(Ref. Strain) |
Vero, C6/36, KC cells
(E2/BHK7) [IAH-BHK2] |
|
* Vial E1(g) supplied to JongChul, Jeong, Ja Yeon Agrotech Co., Ltd., 385-6, Chon Chon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea 19th Dec 2007, * Duplicate samples, position E2(p) supplied to Oscar R. González Llamazares (Avenida Párroco Pablo Díez 49-57, 24010 - Leon - Spain, 22nd Nov 2007 * Vials E3 (g) A&B were sent to Massimo Plamerini, University of Glasgow Dept Veterinary Medicine 1st June 2009. * Vial E5 (g) A&B sent to Mehdi El Harrak, Biopharma , Morocco 29th June 2009 * Vial F1 sent to Denis Kolbasov, Russian National Institute of Veterinary Virology 29th Sept 2009 * Vial F3 (g) from freezer A and B were sent to Pavel Bartak in Czech Republic 20th April 2009 *
Vial F sent to Dr Graham Abdeljelil * [S2:AJ585122, S3: DQ186792, S6:AJ586695 S7: X53740, |
||
|
RSAvvvv/01 (41010D18) |
BTV-1
Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
South African vaccine strain, developed at Onderstepoort Veterinary research Institute |
NK |
Shelf C1, box C1α, rows I1-J10 (Added to collection on 14th Sept 2005) |
South African live vaccine strain |
[IAH/BHK3] |
(Supplied 07/04/2003) |
* duplicate samples J10 a&b (p) given to C.Batten, IAH Pirbright on 12th Oct 2007
* Vial removed from position I2 (g) Freezer A and given to Elizabeth Morecroft for array studies on 10th July 2013. Corresponding vial in Freezer B was not removed . *
[Seg-1:KP820910, Seg-2: KP821017, Seg-3: KP821139, Seg-4: KP821259, Seg-5: KP821379 Seg-6: KP821499, Seg-7: KP821621, Seg-8: KP821741, Seg-9: KP821862, Seg-10: KP821982] * [Seg-2:AJ585110, Seg-5: AM778437, Seg-6: AJ586656 ] |
||
| 2014 | ||||||||||
| SPA2014/08 |
BTV-1
Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Spain |
Isolated in
Laboratoire
Central Verterinaire (LCV) from sheep blood 2014 Ovine Reg. 3487114 sample 10 |
Shelf E7, Box E7α, Tubes D1 to D10 (Added to collection on 10/12/2015) |
BTV1 SPA 2014/02 3P BHK 21/04/15 (Cod. 196) |
Cell culture
KC1 12/03/2015 (4th passage at PI on 03/11/15)
[PI/BHK1] |
Supplied by : Ruben Villalba Martinez
NRL for BTV Spain A127/15 08 |
|||
| 2012 | ||||||||||
| SKR2012/01 |
BTV-1
Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Chungnam province,
South Korea
9 August 2010 |
Cattle ( 2011 , Jee Yong Park, MIFAFF, South Korea) |
Shelf D7 Box D7 β Rows I1-J10
Added to collection on 26th February 2013 |
BHK1 [BHK2 at PIR] |
Sample supplied by Jee Yong Park,
Animal, Plant & Fisheries Quarantine &
Inspection Agency Anyang-ro 175, Anyang-si Gyeonggi South Korea A17/13 02
Received 11/02/13 |
MTA | |||
| 2010 | ||||||||||
|
CRO2010/01 |
BTV-1
Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Croatian quarantine station Oryx blood (oryx imported from Oman) | Isolated in KC cells at IAH-P |
Shelf B17 boxα α, rows I1-J10
(Added to collection on9th April 2010) |
1805/3 HP3 1F4B763C64 | [IAH/KC1] |
Eddy Listes, Croatian veterinary institute
Samples arrived at IAH 23rd March 2010
IAH Stars No.A23/10 03 |
This isolate may be contaminated with bacteria Outbreak data Duplicate vials - position I1(G) given to K. Nomikou (IAH) for sequencing (7th July 2010) |
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| 2009 | ||||||||||
| AUS2009/02 |
BTV-1 |
Australia |
Cow |
Shelf D13, Box D13 β rows F1 to F10 |
Old isolate No.: D439
Sample sent as 'BTV1-21' |
1ECE.1AA, 2BHK21, 1HmLu-1 |
Peter Kirkland, Elizabeth Macarthur Agricultural Institute
|
Note supplied with sample: CPE positive / BT-PCR positive
It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL
(MTA) |
||
| MOR2009/16 |
BTV-1
Identified as BTV-1 by Seg-2 specific Real time RT-PCR and Seg-2 sequence analysis |
Sheep sampled in Fes. Morocco on 18th Sept 2009 |
Isolated from sheep
blood at TPI Pirbright |
Shelf D9 box D9
γ, rows G1-H10
(Added to collection on |
329/09-B2 |
[TPI/KC1BHK1] |
Mehdi El Harrak (Biopharma Laboratory,
BP4569, Rabbat Akkari, Morocco
A94/09 0 |
Outbreak Data * Derived from
MOR2009/10
Distribution restricted |
||
| MOR2009/13 |
BTV1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Sheep sampled in Fes. Morocco on 18th Sept 2009 |
Sheep blood Isolated at IAH Pirbright |
Shelf C16 box C16
γ, rows
C1-D10 (Added to collection on 16th Dec 2011) |
329/09-B2 |
[IAH/ KC2] |
Mehdi El Harrak (Biopharma
Laboratory, BP4569, Rabbat Akkari, Morocco A94/09 01 |
Derived from
by MTA Outbreak data |
||
| MOR2009/11 |
BTV1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Sheep sampled in Marrakech Morocco on 25th Sept 2009 |
sheep
blood Isolated at IAH Pirbright |
Shelf B14 Box B14 β Rows E1-F10
(added to collection on 15th Dec 2009) |
410/09-3 | [IAH/KC2] |
Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat
Akkari, Morocco A94/09 11 |
Outbreak data | ||
| MOR2009/06 |
BTV1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Sheep sampled in Marrakesh. Morocco on 25th Sept 2009 |
sheep blood Isolated at IAH Pirbright |
Shelf B14 Box B14 α Rows A1-B10
(added to collection on 27th Nov 2009) |
341/09-1 |
[IAH/KC1] |
Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco A94/09 06 |
Outbreak data | ||
| MOR2009/05 |
BTV1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Sheep sampled in Fes. Morocco
on 25th Sept 2009 |
Sheep blood Isolated at IAH Pirbright |
Shelf B13 Box B13 γ Rows
I1-J10
(added to collection on 27th Nov 2009) |
339/09-T3 | [IAH/ KC1] |
Mehdi El Harrak (Biopharma Laboratory,
BP4569, Rabbat Akkari, Morocco A94/09 05 |
Outbreak data | ||
| MOR2009/04 |
BTV1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Sheep sampled in Fes. Morocco on 25th Sept 2009 |
Sheep blood Isolated at IAH Pirbright |
Shelf B13 Box B13 γ Rows G1-H10
(added to collection on 27th Nov 2009) |
339/09-T1 | [IAH/ KC1] |
Mehdi El Harrak (Biopharma Laboratory,
BP4569, Rabbat Akkari, Morocco |
Outbreak data | ||
| MOR2009/03 |
BTV1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Sheep sampled in Fes. Morocco
on 25th Sept 2009 |
Sheep blood Isolated at IAH Pirbright |
Shelf B13 Box B13 γ Rows
E1-F10
(added to collection on 27th Nov 2009) |
338/09-1 |
[IAH/ KC1] |
Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco
A94/09 03 |
Outbreak data | ||
| MOR2009/02 |
BTV1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Sheep sampled in Fes. Morocco
on 18th Sept 2009 |
Sheep blood Isolated at IAH Pirbright |
Shelf B13 Box B13 γ Rows
C1-D10
(added to collection on 27th Nov 2009) |
329/09-B1 |
[IAH/ KC1] |
Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco |
Outbreak data | ||
| MOR2009/01 |
BTV1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR |
Sheep sampled in Fes. Morocco on 18th Sept 2009 |
Sheep blood Isolated at IAH Pirbright |
Shelf B13 Box B13 γ Rows
A1-B10
(added to collection on 27th Nov 2009) |
329/09-B2 |
[IAH/ KC1] |
Mehdi El Harrak (Biopharma
Laboratory, BP4569, Rabbat Akkari, Morocco A94/09 01 |
* Vial positions A1 (G) from freezer A and B given to K. Nomikou (IAH-P) 15th Apr 2010 Duplicate vials, positions A2 (g) given to K. Nomikou (IAH-P) 20th May 2010 for sequencing * Duplicate vial positions A10 (G) sent to Marta Vigo Martin, Algete Spain on 15th Dec 2009 under MTA * Duplicate vials, position B1 given to C. Batten (IAH-P) 28th April 2010 for Ring Trial Used to generate MOR2009/13 Outbreak data |
||
| OMN2009/08 |
BTV-1
Identified as BTV by Seg-7 specific Real time RT-PCR (Typed by: Seg-2 real-time RT-PCR ) |
Oman from Sohar, Batnah coast in the
Arabian Gulf Sample collected 27th April 2009 |
Sheep blood Isolated through KC cells at IAH-P |
Shelf B9 Box B9 BETA Rows E1- F10 (Added to collection 16th June 2009) |
Oman designation F1114 |
[IAH/KC1] |
Masha Japhet, Oman
Samples arrived Approx 15TH May 2009
IAH Stars number A42/09 41 |
Not all +ve animals showed clinical signs of disease. Only Wiltshire sheep,
Arabian gazelles, fallow deer and blackbucks were affected . Only one
multi-horn sheep died, showing similar signs and post mortem changes, but
all of the other live animals sampled were -ve. The +ve Cattle, buffalo,
goats and reem gazelles showed no signs of disease . Strains of BTV-4, BTV-8 and BTV-16 were also isolated in early 2009 (see: OMN Isolates) |
||
| OMN2009/06 |
BTV-1
Identified as BTV by Seg-7 specific Real time RT-PCR (Typed by: Seg-2 real-time RT-PCR ) |
Oman from Sohar, Batnah coast in the
Arabian Gulf |
Sheep blood Isolated through KC cells at IAH-P |
Shelf B9 Box B9 BETA Rows A1- B10(Added to collection 16th June 2009) |
Oman designation E724 | [IAH/KC1] |
Masha Japhet, Oman
Samples arrived Approx 15TH May 2009
IAH Stars No.A42/09 39 |
not all +ve animals showed clinical signs of disease. Only Wiltshire sheep,
Arabian gazelles, fallow deer and blackbucks were affected . Only one
multi-horn sheep died, showing similar signs and post mortem changes, but
all of the other live animals sampled were -ve. The +ve Cattle, buffalo,
goats and reem gazelles showed no signs of disease . Strains of BTV-4, BTV-8 and BTV-16 were also isolated in early 2009 (see: OMN Isolates) |
||
| OMN2009/05 |
BTV-1
Identified as BTV by Seg-7 specific Real time RT-PCR(Typed by: Seg-2 real-time RT-PCR ) |
Oman from Sohar, Batnah coast in the
Arabian Gulf Sample collected 29th Apr 2009 |
Sheep blood Isolated in KC cells at IAH-P |
Shelf B9 Box B9 alpha Rows I1- J10(Added to collection 16th June 2009) |
Oman designation C1407 | [IAH/KC1] |
Masha Japhet, Oman
Samples arrived 15TH May 2009
IAH Stars No.A42/09 38 |
not all +ve animals showed clinical signs of disease. Only Wiltshire sheep,
Arabian gazelles, fallow deer and blackbucks were affected . Only one
multi-horn sheep died, showing similar signs and post mortem changes, but
all of the other live animals sampled were -ve. The +ve Cattle, buffalo,
goats and reem gazelles showed no signs of disease . Strains of BTV-4, BTV-8 and BTV-16 were also isolated in early 2009 (see: OMN Isolates) |
||
| OMN2009/01 |
BTV-1
Identified as BTV by Seg-7 specific Real time RT-PCR (Typed by: Seg-2 real-time RT-PCR ) |
Oman from Sohar, Batnah coast in the
Arabian Gulf Sample collected 20th Apr 2009 |
Sheep blood
Isolated in KC cells at IAH-P |
Shelf B9 Box B9 alpha Rows Added A1- B10(Added to collection 16th June 2009) |
Oman designation C1414 | [IAH/KC1] |
Masha Japhet, Oman
Samples arrived 15TH May 2009
[IAH Stars No.
A42/09 19] |
not all +ve animals showed clinical signs of disease. Only Wiltshire sheep,
Arabian gazelles, fallow deer and blackbucks were affected . Only one
multi-horn sheep died, showing similar signs and post mortem changes, but
all of the other live animals sampled were -ve. The +ve Cattle, buffalo,
goats and reem gazelles showed no signs of disease . Strains of BTV-4, BTV-8 and BTV-16 were also isolated in early 2009 (see: OMN Isolates) * Duplicate vials, position A1 given to C. Batten (IAH-P) 28th April 2010 for Ring Tria * Vial position B1(p) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013. Corresponding vial in Freezer B has not been removed. * |
||
| SPA2009/01 |
BTV-1
Identified as BTV-1 by Seg-2 specific Real time RT-PCR in Spain |
Spain Galicia Sample collected 2009 |
Cattle blood Isolated at LCV Spain |
Shelf C11, box C11 α, rows E1-F10 |
4852/09 sample 10 |
E1/C636-1/ BHK4 [IAH/BHK1] |
Marta Vigo Martin |
|||
| 2008 | ||||||||||
| ALG2008/16 |
BTV-1
Identified by BTV
group-specific RT-PCR Typed by
|
ALGERIA M’SILA Khoubana Maadhar
(Lat: 35,74 Long:e 4,531) Start of outbreak
29th Oct 2008 |
Isolated from whole sheep blood, in KC cells at IAH Pirbright |
Shelf
B14,
box B14β
,
rows A1-B10
Added
to collection 9th Dec 2009 |
Animal No 5283/003 |
[IAH KC2] |
From
Hafsa Madani, Ministere de L’Agriculture et du Developpement Rural, Institiut National de la Medicine Veterinaire [A 172/08 03] Received at IAH 1st Dec 2008 |
|||
| ALG2008/15 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
Confirmed by Seg- 2 sequencing |
Algeria, Laghouat Dakhla (Lat: 33,849 Long: 2,834)
Outbreak
2nd Nov 2008
Received at IAH |
Isolated from
whole sheep
blood at
IAH Pirbright
|
Shelf B12,
box B12γ ,
rows G1-H10
Added to
collection 6th Nov 2009 |
Animal No: TALEB MOHAMED 05 |
[IAH KC1/BHK1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 12] |
Isolate derived from ALG2008/14 |
||
| ALG2008/14 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
Confirmed by Seg- 2 sequencing |
Algeria, Laghouat Dakhla (Lat: 33,849 Long: 2,834)
Outbreak
2nd Nov 2008 Received at IAH |
Isolated from
whole sheep
blood at
IAH Pirbright
|
Shelf B12,
box B12γ ,
rows E1-F10
Added to
collection 6th Nov 2009 |
Animal No: TALEB MOHAMED 05 |
[IAH/KC1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 12] |
Isolate used to generate |
||
| ALG2008/13 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
Confirmed by Seg- 2 sequencing |
Algeria, Laghouat Dakhla (Lat: 33,849 Long: 2,834)
Outbreak
2nd Nov 2008 Received at IAH |
Isolated from
whole sheep
blood at
IAH Pirbright
|
Shelf B12,
box B12γ ,
rows C1-D10
Added to
collection 6th Nov 2009 |
Animal No: SAHRAOUI DJAMEL 04 |
[IAH/KC1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 11] |
|||
| ALG2008/12 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
conventional RT-PCR and specific real-time PCR
|
Algeria, Laghouat Dakhla (Lat: 33,849 Long: 2,834)
Outbreak
2nd Nov 2008 Received at IAH |
Isolated from
whole sheep
blood at
IAH Pirbright
|
Shelf B12,
box B12γ ,
rows A1-B10
Added to
collection 6th Nov 2009 |
Animal No: BELBOUL MOHAMED 03 |
[IAH/KC1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 10] |
|||
| ALG2008/11 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
Confirmed by Seg- 2 sequencing |
Algeria, Laghouat Dakhla (Lat: 33,849 Long: 2,834)
Outbreak
2nd Nov 2008 Received at IAH |
Isolated from whole sheep blood at IAH Pirbright |
Shelf B12,
box B12β ,
rows I1-J10
Added to
collection 6th Nov 2009 |
Animal No BELBOUL MOHAMED 02 |
[IAH/KC1] |
Hafsa MADANI, Ministere de L’Agriculture et du Developpement Rural, Institiut National de la Medicine Veterinaire [A 172/08 09] |
|||
| ALG2008/10 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
Confirmed by Seg- 2 sequencing |
Algeria, Laghouat Dakhla (Lat: 33,849 Long: 2,834)
Outbreak
2nd Nov 2008
Received at IAH
Probable vector C.imicola |
Isolated from
whole sheep
blood (animal died) at
IAH Pirbright
|
Shelf B12,
box B12β ,
rows G1-H10
Added to
collection 6th Nov 2009 |
Animal No: NIA SAADI 01 |
[IAH/ KC1/BHK1] |
Hafsa MADANI, Ministere de L’Agriculture et du Developpement Rural, Institiut National de la Medicine Veterinaire [A 172/08 08]
Sequenced at KC1/BHK1 |
Isolate derived from ALG2008/09
[ Seg-1 KP820877; Seg-2 KP820997; Seg-3 KP821119; Seg-4 KP821239; Seg-5 KP821359; Seg-6 KP821479; Seg-7 KP821601; Seg-8 KP821721; Seg- 9 KP821842; Seg-10 KP821962; |
||
| ALG2008/09 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
conventional RT-PCR and specific real-time PCR
|
Algeria, Laghouat Dakhla (Lat: 33,849 Long: 2,834)
2nd Nov 2008 Received at IAH |
Isolated from whole sheep blood at IAH Pirbright |
Shelf B12,
box B12β ,
rows E1-F10
Added to
collection 6th Nov 2009 |
Animal No: NIA SAADI 01 |
[IAH/KC1] |
Hafsa MADANI, Ministere de L’Agriculture et du Developpement Rural, Institiut National de la Medicine Veterinaire [A 172/08 08] |
Isolate used to generate ALG2008/10 |
||
| ALG2008/08 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
Confirmed by Seg- 2 sequencing |
Algeria M'Sila, Ain Fares Ouled Attia
(Lat: 35,74 Long: 4,531)
29th Oct 2008 Received at IAH |
Isolated from
whole sheep
blood at
IAH Pirbright
|
Shelf B12,
box B12β ,
rows C1-D10
Added to
collection 6th Nov 2009 |
Animal No 28/304 |
[IAH/KC1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 07] |
Outbreak
29th Oct 2008 |
||
| ALG2008/07 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
Confirmed by Seg- 2 sequencing |
Algeria M'Sila, Ain Fares Ouled Attia
(Lat: 35,74 Long: 4,531)
29th Oct 2008 Received at IAH |
Isolated from
whole sheep
blood at
IAH Pirbright
|
Shelf B12,
box B12β ,
rows A1-B10
Added to
collection 6th Nov 2009 |
Animal No 28/302 |
[IAH KC1/BHK1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 06] |
Isolate derived from ALG2008/06 |
||
| ALG2008/06 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
Confirmed by Seg- 2 sequencing |
Algeria M'Sila, Ain Fares Ouled Attia
(Lat: 35,74 Long: 4,531)
29th Oct 2008 Received at IAH |
Isolated from
whole sheep
blood at
IAH Pirbright |
Shelf B12,
box B12α ,
rows I1-J10
Added to
collection 6th Nov 2009 |
Animal No 28/302 |
[IAH/KC1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 06] |
Isolate used to generate ALG2008/07 |
||
|
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
conventional RT-PCR and specific real-time PCR
|
Algeria M'Sila, Ain Fares Ouled Attia
(Lat: 35,74 Long: 4,531)
29th Oct 2008 Received at IAH |
Isolated from whole sheep blood at IAH Pirbright |
Shelf B12,
box B12α ,
rows G1-H10
Added to
collection 6th Nov 2009 |
Animal No 28/301 |
[IAH/KC1] |
Hafsa MADANI, Ministere de L’Agriculture et du Developpement Rural, Institiut National de la Medicine Veterinaire [A 172/08 05] |
||||
|
BTV-1Identified by BTV
group-specific
RT-PCR [3]
Typed by
conventional RT-PCR and specific real-time PCR
|
Algeria M'Sila, Ain Fares Ouled Attia
(Lat: 35,74 Long: 4,531)
Outbreak
29th Oct 2008 Received at IAH |
Isolated from
whole sheep
blood at
IAH Pirbright
|
Shelf B12,
box B12α ,
rows E1-F10
Added to
collection 6th Nov 2009 |
Animal No 28/300 |
[IAH KC1/BHK1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 04] |
Isolate derived from |
|||
| ALG2008/03 |
BTV-1
Identified by BTV
group-specific
RT-PCR [3]
Typed by
Confirmed by Seg- 2 sequencing |
Algeria M'Sila, Ain Fares Ouled Attia
(Lat: 35,74 Long: 4,531)
Outbreak
29th Oct 2008 1st Dec 2008 |
Isolated from whole sheep blood at IAH Pirbright |
Shelf B12,
box B12α ,
rows C1-D10
Added to
collection 6th Nov 2009 |
Animal No 28/300 |
[IAH KC1] |
Hafsa MADANI, Ministere de L’Agriculture et du Developpement Rural, Institiut National de la Medicine Veterinaire [A 172/08 04] |
Isolate used to generate ALG2008/04 |
||
| ALG2008/02 |
BTV-1
Identified by BTV group-specific
RT-PCR [3] Typed by
Confirmed by Seg- 2 sequencingg |
Algeria M'Sila, Khoubana Maadhar
(Lat: 35,74 Long: 4,531)
Outbreak
29th Oct 2008 1st Dec 2008 |
Isolated from
whole sheep
blood at
IAH Pirbright
|
Shelf B11,
box B11γ ,
rows I1-J10
Added to
collection 6th Nov 2009 |
Animal No 5283/004 |
[IAH KC1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 01] |
|||
|
BTV-1
Identified by BTV group-specific
RT-PCR [3] Typed by
conventional RT-PCR and specific real-time PCR
|
Algeria M'Sila, Khoubana Maadhar
(Lat: 35,74 Long: 4,531)
Outbreak
29th Oct 2008
Received at IAH |
Isolated from
whole sheep
blood at
IAH Pirbright
|
Shelf B12,
box B12α ,
rows A1-B10
Added to
collection 6th Nov 2009 |
Animal No 5283/002 |
[IAH KC1] |
Hafsa MADANI,
Ministere de
L’Agriculture et
du Developpement
Rural, Institiut
National de la
Medicine Veterinaire
[A 172/08 02] |
||||
| FRA2008/26 | BTV-1 |
From France 2008 |
Isolated at AFSSA- Maisons Alfort France in 2008 |
Shelf C9,
box C9
γ
,
rows G1-H10
Added to collection 15th Sept 2010 |
Original tube labelled as BTV1.08.06 |
[IAH/BHK1] |
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material | from an unvaccinated animal | ||
| FRA2008/25 | BTV-1 |
From France 2008 |
Isolated at AFSSA- Maisons Alfort France in 2008 |
Shelf C9,
box C9
γ
,
rows E1-F10
Added to collection 15th Sept 2010 |
Original tube labelled as BTV1.08.05 |
[IAH/BHK1] |
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material | from an unvaccinated animal | ||
| FRA2008/24 | BTV-1 |
From France 2008 |
Isolated at AFSSA- Maisons Alfort France in 2008 |
Shelf C9,
box C9
γ
,
rows C1-D10
Added to collection 15th Sept 2010 |
Original tube labelled as BTV1.08.04 | [IAH/BHK1] | Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material | from an unvaccinated animal | ||
| FRA2008/23 | BTV-1 |
From France 2008 |
Isolated at AFSSA- Maisons Alfort France in 2008 |
Shelf C9,
box C9
γ
,
rows A1-B10
Added to collection 15th Sept 2010 |
Original tube labelled as BTV1.08.03 |
[IAH/BHK1] |
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material | from an unvaccinated animal | ||
| FRA2008/22 | BTV-1 |
From Pinderes-Lot et Garonne-Aquitaine France 2008 Vector insects in the area C.obsoletus |
Isolated from Sheep blood at AFSSA- Maisons Alfort France in 2008 |
Shelf C9,
box C9
β
,
rows I1-J10
Added to collection 15th Sept 2010 |
Original tube labelled as BTV1.08.02 |
From unvaccinated sheep blood sample
E1/BHK1 [IAH/BHK1] |
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as
cell culture material
Sequenced at E1 BHK3 |
from an unvaccinated animal [ Seg-1 KP820880; Seg-2 KP821000; Seg-3 KP821122; Seg-4 KP821242; Seg-5 KP821362; Seg-6 KP821482; Seg-7 KP821604; Seg-8 KP821724; Seg-9 KP821845; Seg-10 KP821965; |
||
| FRA2008/21 | BTV-1 |
Beyrie-en-Béarn- Pyrenees Atlantiques-Aquitaine,
France 2008 Vector insects in the area C.obsoletus |
Isolated from a cattle blood sample at AFSSA- Maisons Alfort France in 2008 |
Shelf C9,
box C9
β
,
rows G1-H10
Added to collection 15th Sept 2010 |
Original tube labelled as BTV1.08.01 |
From cattle blood sample
E1 BHK3 [IAH/BHK1] |
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as
cell culture material
Sequenced at E1 BHK5 |
from an unvaccinated animal [ Seg-1 KP820879; Seg-2 KP820999; Seg-3 KP821121; Seg-4 KP821241; Seg-5 KP821361; Seg-6 KP821481; Seg-7 KP821603; Seg-8 KP821723; Seg-9 KP821844; Seg-10 KP821964; |
||
| SPA2008/01 |
BTV-1
Identified as BTV-1 by Seg-2 specific Real time RT-PCR in Spain |
Spain Asturias Sample collected in 2008 |
Sheep blood Isolated at LCV Spain |
Shelf C11, box C11 α, rows A1-B10 collection on 13th Dec 2010 |
5644/2008 sample 1 |
E1/C636-1/ BHK3 [IAH/BHK1] |
Marta Vigo Martin |
|||
| 2007 | ||||||||||
| FRA2007/18 |
BTV-1 Identified by sequence analysis of Seg-2 |
From Sare- Labourd-Pyrénées-Atlantiques-Aquitaine,
France From unvaccinated goat Vector insects in the area C.obsoletus |
isolated from a goat spleen sample at AFSSA- Maisons Alfort France in 2007 |
Shelf C9,
box C9
β
,
rows C1-D10
Added to collection 13th Sept 2010 |
Original tube marked as: BTV1-07.01 Rate d 64 Lot 4 |
E1/BHK2 [IAH/BHK1 ] |
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010
as cell culture material Note: this is the only isolate of BTV-1 made in France during 2007 Sequenced at E1/BHK4 |
MTA [ Seg-1 JX861488; Seg-2 JX861489; Seg-3 JX861490; Seg-4 JX861491; Seg-5 JX861492; Seg-6 JX861493; Seg-7 JX861494; Seg-8 JX861495; Seg- 9 JX861496; Seg-10 JX861487; |
||
| GIB2007/06 |
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 [2] |
Gibraltar Isolated from adult, male sheep blood.
(sample taken 5th Oct 2007) |
Virus isolated at IAH Pirbright October 2007 |
(added to collection on 20th May 2009) |
GIB1 | [IAH/KC2] |
Mark Pizarro (Vet) [A106/07 1] |
Derived from
GIB2007/01 * 500ml sample sent to Veronique Moulin Intervet, Boxmeer the Netherlands for vaccine challenge studies May 20th 2009 * outbreak data |
||
| GIB2007/05 |
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2) |
Gibraltar
Isolated from adult, female goat blood. Sample taken 15th Oct 2007 |
Virus isolated at IAH Pirbright October 2007 |
Shelf: E6 Box E6 Gamma Rows C1-D10 Date stored: 08/11/07 |
GG7 | [IAH/E1/BHK2] |
Mark Pizarro (Vet)
[A111/07 11] |
* Vial position C1 was removed and given to C. Batten IAH-P, on 25th Aug 2009 to grow material for VLA for PCR validation, Duplicate vial left in Misc box position H7 * outbreak data |
||
| GIB2007/04 |
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2) |
Gibraltar
Isolated from adult, female goat blood. Sample taken 15th Oct 2007 |
Virus isolated at IAH Pirbright October 2007 |
Shelf: E6 Box E6 Gamma Rows A1-B10 Date stored: 08/11/07 |
GG5 | [IAH/E1/BHK2] |
Mark Pizarro (Vet) [A111/07 9] |
From same sample as
GIB2007/03 outbreak data |
||
| GIB2007/03 |
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2) |
Gibraltar
Isolated from adult, female goat blood. (sample taken 15th Oct 2007) |
Virus isolated at IAH
Pirbright October 2007 |
Shelf E6, box E6 α, rows E1-F10
(added to collection on 29th Oct 2007) |
GG5 | [IAH/KC1] |
Mark Pizarro (Vet)
[A111/07 9] |
Note F10
(p) only 0.5 ml From same sample as GIB2007/04 |
||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2) |
Gibraltar Isolated from adult, female sheep blood.
(sample taken 5th Oct 2007) |
Virus isolated at IAH Pirbright October 2007 |
Shelf E6, box E6 α, rows C1-D10
(added to collection on 29th Oct 2007) |
GIB2 |
[IAH/KC1] |
Mark Pizarro (Vet)
[A106/07 2] |
||||
| GIB2007/01 |
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2) |
Gibraltar Isolated from adult, male sheep blood.
(sample taken 5th Oct 2007) |
Virus isolated at IAH Pirbright October 2007 |
Shelf E6, box E6 α, rows A1-B10
(added to collection on 29th Oct 2007) |
GIB1 | [IAH/KC1] |
Mark Pizarro (Vet)
[A106/07 1] |
Note B10 (p)
only 0.5 ml * Vial A2(g) (from Freezer A) was given to Eva Veronesi (IAH Pirbright) on 15th Dec 2008, to prepare a vaccine challenge strain (KC2) for Intervet - Spare vial Position A2 (freezer B) was given to Karin Darpel (IAH) 18th May 2010 * Vial position A3 (g) freezer A was given to Eva Veronesi (IAH Pirbright on 6th Nov 2009 for Ph.D studies) - Second vial position A3 was given to K. Nomikou 24th Nov 2010 for sequencing
Vial Position A4 (freezer A) was given to E. Veronesi (IAH) 13th Dec 2010. Corresponding 'Spare' vial in freezer B was not removed. *
Vial position
A5 was
taken out of Freezer A and given to Rhiannon Silk (Pirbright)
for the Genome project *
|
||
| MOR2007/06 |
BTV-1 Identified as BTV-1 by
type-specific RT-PCR targeting Seg-2 |
Morocco
(Blood sample taken from sheep 2nd July
2007) |
Heparin treated sheep
Blood (isolated at TPI
Pirbright) |
Shelf D13, Box D13 α, rows B1 to B10
(Added to
collection on 31st July 2014) |
OV 4156 Foci 2007 |
[IAH/KC1BHK1] |
Mehdi El
Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco - arrived 5th Feb
2008) |
|||
| MOR2007/05 |
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 |
Morocco
(Blood sample taken from sheep 2nd July
2007) |
Heparin treated sheep
Blood (isolated at TPI
Pirbright) |
Shelf D13, Box D13 α, rows A1 to A10
(Added to
collection on 31st July 2014) |
HAM 5 B Foci 2007 |
[IAH/KC1BHK1] |
Mehdi El
Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco - arrived 5th Feb
2008) |
|||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2), and Seg-2 sequence analyses (4) |
Kenitra,
Morocco
(Blood sample taken from sheep 2nd July 2007) |
Heparin treated sheep Blood
(isolated at IAH Pirbright) |
Shelf A9, box A9 α, rows G1-H10
(added to collection on 12th March 2008) |
46/07 Foci 2007 |
[IAH/KC1] |
Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco - arrived 5th Feb 2008) |
||||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2), and Seg-2 sequence analyses (4) |
Rabat, Morocco
(Blood sample taken from sheep 2nd July 2007) |
EDTA treated sheep blood
(isolated at IAH Pirbright) |
Shelf A9, box A9 α, rows A1-B10
(added to collection on 10th Mar 2008) |
DR12/07 Foci 2007 |
[IAH/KC1] |
Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco - arrived 5th Feb 2008) |
|
|||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2), and Seg-2 sequence analyses (4) |
Settat, Morocco
(Blood sample taken from sheep 19th July 2007) |
EDTA treated sheep blood
(isolated at IAH Pirbright) |
Shelf A9, box A9 α, rows C1-D10
(added to collection on 10th Mar 2008) |
SETT/07 Foci 2007 |
[IAH/KC1] |
Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco - arrived 5th Feb 2008) |
|
|||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2), and Seg-2 sequence analyses (4) |
Khouribga, Morocco
(Blood sample taken from sheep 2nd July 2007) |
Heparin Blood
(isolated at IAH Pirbright) |
Shelf A9, box A9 α, rows E1-F10
(added to collection on 10th Mar 2008) |
02/07 Foci 2007 |
[IAH/KC1] |
Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco - arrived 5th Feb 2008) |
Sample E1 given to Anna Swain IAH on 16th May 2008 Spare single sample stored as *
|
|||
| SPA2007/05 |
BTV-1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR in Spain |
Spain Pias Vasco 2007 |
Sheep Blood Isolated at LCV Spain |
Shelf C11 box C11 α, rows G1-H10 collection on 13th Dec 2010 |
9537/2007 sample 146 |
E1/C636-1/ BHK2 [IAH/BHK1] |
Marta Vigo Martin |
|||
| SPA2007/04 |
BTV-1 Identified as BTV-1 by Seg-2 specific Real time RT-PCR in Spain |
Spain Cordoba 2007 |
Cattle Blood Isolated at LCV Spain |
Shelf C11, box C11 α, rows C1-D10 collection on 13th Dec 2010 |
8137/2007 sample 1 |
E1/C6/36-1/ BHK3 [IAH/BHK1] |
Marta Vigo Martin |
|||
|
BTV-1 Typed At LCV, Algete, Spain
Typed by real time RT-PCR |
Extremadura Spain Sample taken September 2007 |
From cattle blood |
Shelf A11 Box A11 β, E1-F10
Added to collection on 11th June 2008 |
|
E1/C636-1 /BHK2
[+IAH/BHK1] |
Concha Gomezte,
Laboratorio Central de Veterinaria
km. 8 |
derived from: original isolate |
|||
|
BTV-1 Typed At LCV, Algete, Spain
Typed by real time RT-PCR |
Castilla –La Mancha Spain Sample taken October 2007 |
From cattle blood |
Shelf A11 Box A11 β, C1-D10
Added to collection on 11th June 2008 |
|
E1/C636-1 /BHK2
[+IAH/BHK1] |
Concha Gomezte,
Laboratorio Central de Veterinaria
km. 8 |
derived from: original isolate |
|||
|
BTV-1 Typed At LCV, Algete, Spain
Typed by real time RT-PCR and sequence analysis of 400bp of Seg-2 |
Andalucia Spain
Sample taken July 2007
|
From sheep blood |
Shelf A11, box A11 β, rows A1-B10
Added to collection on 11th June 2008 |
|
E1/C636-1 /BHK2
[+IAH/BHK1] |
Concha Gomezte,
Laboratorio Central de Veterinaria
km. 8 |
derived from: original isolate |
|||
| TUN2007/02 |
BTV-1
Identified by
serotype specific (Seg-2)
RT-PCR
(2)
and sequence
analyses
(3 |
Tunisia sample collected 2007 |
Isolated from
sheep blood at TPI Pirbright 18th Jan 2008 |
Shelf C17 box C17 α, Rows G1 to H10
&
Shelf D9 box D9 gamma, Rows I1 to J10 (Added to collection on 18th Mar 2014) |
[TPI/KC2] |
Sample supplied by Dr. Hammamai Salah, Ministere de l’agriculture Tunisia [A2/08 1] |
Outbreak Data * Derived from
TUN2007/01 |
|||
|
BTV-1 Identified as BTV by rt-RT-PCR targeting Seg-1 (3) |
From Sheep blood Tunisia 2007 |
Isolated at IAH Pirbright 18th Jan 2008 |
Shelf A8 box A8 α, Rows I1-J10
Added to collection on 25th Jan 2008 |
[IAH/KC1] |
Sample supplied by Dr. Hammamai Salah, Ministere de l’agriculture Tunisia [A2/08 1] |
Sample I1 freezer A given to Anna Swain (Pirbright) on 16th May 2008. Spare single sample stored as Misc TUN2007/01 * Tubes I2 removed from freezer A&B * Tubes J1 removed from freezer A&B * |
||||
|
BTV-1
identified as BTV by real time RT-PCR (Seg 1) (3)
Identified as type 1 by serotype specific RT-PCR (Seg -2) (2) |
Green Mountain, East Libya
sample collected 2nd Aug 2007 |
EDTA treated lysed sheep blood
virus isolated at the CRL IAH Pirbright October 2007 |
Shelf E5, box E5 β, rows G1- H10
Added to collection on 15th Oct 2007 |
C4 |
[KC1/BHK1] |
A99/07 – 13 Jimmy Elaref National center of the animal health and breeder Tripoli -Libya |
|
|||
|
BTV-1
identified as BTV by real time RT-PCR (Seg 1) (3)
Identified as type 1 by serotype specific RT-PCR (Seg -2) (2) |
Green Mountain, East Libya 02/08/2007
|
Ovine lysed EDTA blood Isolated through KC cells at CRL |
helf E5 box E5 BETA positions E1-F10
ADDED 15/10/07
|
C1 |
KC1/BHK1 |
Jimmy Elaref National center of the animal health and breeder improvement Tripoli -Libya
[A99/07 – 10] |
|
|||
|
BTV-1
identified as BTV by real time RT-PCR (Seg 1) (3)
Identified as type 1 by serotype specific RT-PCR (Seg -2) (2) |
Green Mountain, East Libya 02/08/2007
|
Ovine lysed EDTA blood Isolated through KC cells at CRL |
Shelf E5 box E5 BETA positions C1- D9 NO D10 (PDAM) ADDED 15/10/07
|
B1 |
KC1/BHK1 |
Jimmy Elaref National center of the animal health and breeder improvement Tripoli -Libya
[A99/07 – 6] |
||||
|
BTV-1
identified as BTV by real time RT-PCR (Seg 1) (Shaw et al 2007)
Identified as type 1 by serotype specific RT-PCR (Seg -2)(Mertens et al 2007) |
Green Mountain, East Libya 02/08/2007
|
Ovine lysed EDTA blood Isolated through KC cells at CRL
|
Shelf E5 box E5 BETA positions A1- B10
ADDED 15/10/07
|
A3 |
KC1/BHK1 |
Jimmy Elaref National Centre of the animal health and breeder improvement Tripoli -Libya
[A99/07 – 3] |
|
|||
|
identified as BTV by real-time RT-PCR (Seg 1: |
Green Mountain, East Libya
Collected on 2nd Aug 2007 |
lysed EDTA treated sheep blood
Isolated in KC cells at CRL - IAH Pirbright |
Shelf E4, box E4 γ, rows G1-8 H1-8
Added to collection on 9th Oct 2007 |
C4 |
KC1 |
[A99/07 – 13] |
|
|||
|
identified as BTV by real-time RT-PCR (Seg 1: |
Green Mountain, East Libya
Collected on 2nd Aug 2007 |
lysed EDTA treated sheep blood
Isolated in KC cells at CRL - IAH Pirbright |
Shelf E2, box E2 α, rows I1-8 J1-8
Added to collection on 9th Oct 2007 |
C1 |
KC1 |
[A99/07 - 10] |
|
|||
|
identified as BTV by real-time RT-PCR (Seg 1: |
Green Mountain, East Libya
Collected on 2nd Aug 2007 |
lysed EDTA treated sheep blood
Isolated in KC cells at CRL - IAH Pirbright |
Shelf E2, box E2 α, rows G1-8 H1-8
Added to collection on 9th Oct 2007 |
B1 |
KC1 |
[A99/07 – 6] |
|
|||
| LIB2007/01 |
identified as BTV by real-time RT-PCR (Seg 1: |
Green Mountain, East Libya
Collected on 2nd Aug 2007 |
lysed EDTA treated sheep blood
Isolated in KC cells at CRL - IAH Pirbright |
Shelf E2, box E2 α, rows E1-8 F1-8
Added to collection on 9th Oct 2007 |
A3 |
[IAH/KC1] |
[A99/07 - 3] |
* Vial position E1(g) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013. Corresponding vial in Freezer B has not been removed. * |
||
| 2006 | ||||||||||
| ALG2006/06 |
BTV1 Confirmed by RT-PCR Seg 2 sequencing |
Algeria Berrouaghia 23rd/24th Aug 2006 |
Isolated from whole sheep blood at IAH Pirbright |
Shelf D2, box D2 γ rows G1-H10 (added to collection 9th Nov 2006) |
No10 |
[KC2/BHK1] |
Hafsa MADANI, Ministere de L’agriculture et du Developpement Rural, Institiut National de la Medicine Veterinaire [A89/06 6] |
related to sample ALG2006/04
|
||
|
BTV Type 1
|
Algeria El Bayadh 23rd-24th Aug 2006 |
Isolated from whole sheep blood at IAH Pirbright |
Shelf D2, box D2 γ rows C1-D10 (added to collection 9th Nov 2006) |
No32/ov5 |
[IAH/ KC2/BHK1] |
Hafsa MADANI Ministere de L’agriculture et du Developpement Rural, Institiut National de la Medicine Veterinaire [A89/06 No.9] |
|
|||
| ALG2006/04 |
BTV Type 1
|
Algeria
Aug 2006 |
Isolated from whole sheep blood at IAH Pirbright |
Shelf D2, box D2 γ rows E1-F10 (added to collection 9th Nov 2006) |
No10 |
[IAH/KC3] related to sample ALG2006/06 |
Hafsa MADANI Ministere de L’agriculture et du Developpement Rural, Institiut National de la Medicine Veterinaire [A89/06 No.6] |
Vial position E2(P) freezer A given to Karin.Darpel and Katy Moffat (Pirbright) on 21st Feb 2012. Eequivalent vial from Freezer B was not removed
[ Seg-1 KP820876; Seg-2 KP820996; Seg-3 KP821118; Seg-4 KP821238; Seg-5 KP821358; Seg-6 KP821478; Seg-7 KP821600; Seg-8 KP821720; Seg- 9 KP821841; Seg-10 KP821961; |
||
|
BTV Type 1 |
Algeria El Bayadh
Sample taken 23rd/24th August 2006 |
Whole sheep blood Isolated at IAH Pirbright |
Shelf D2 Box D2 gamma Rows I1-J10
(added to collection on 10th Nov 2006) |
No32/ov5 |
[IAH/KC2] |
Supplied by: Hafsa Madani Ministere de L’agriculture et du Developpement Rural Institiut National de la Medicine Veterinaire [A89/06 No. 9] |
|
|||
|
BTV Type 1
(Identified as BTV by Seg-7 Group specific RT-PCR, Typed by: , Seg-2 RT-PCR) |
Berrouaghia Media Algeria
Probable vector C.imicola |
Isolated at IAH Pirbright from Sheep spleen (post-mortem), by inoculation into ECE on 24th September 2006 |
Shelf D1, box D1γ, rows E1-F10
(added to collection on 28th Oct 2006) |
(no 3) |
[IAH/E1/BHK2] |
supplied by Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco - arrived at TPI 24th Sept 2006
(R11/06-1)
[S2: EU625361 S6: EU422952]
[ Seg-1 KP820875; Seg-2 KP820995; Seg-3 KP821117; Seg-4 KP821237; Seg-5 KP821357; Seg-6 KP821477; Seg-7 KP821599; Seg-8 KP821719; Seg- 9 KP821840; Seg-10 KP821960; |
* Duplicate samples, box D1γ- position E1(p) supplied to Concepcion Gomez-Tejedor (Madrid) 30th Oct 2006 * Duplicate samples, box D1γ- position F1 supplied to Giovanni Savini (IZS Teramo, 4th Dec 2006) * Single sample, box D1γ- position E2 (pdam) supplied to Miguel Ángel Jiménez C.I.S.A. Algete valdeolmos Madrid, Spain 25th May 2007- second vial kept as misc.) * Duplicate samples, box D1γ- position E3(p) supplied to Carolin Schumacher (Merial Lyon 22nd Nov 2007) * Sample F2 given to Anna Swain IAH on 16th May 2008 (Duplicate tube left in Shelf E1, Box E1β) * Spare single sample stored as
|
|||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2), and Seg-2 sequence analyses (4) |
Oujda, Morocco
(Sample taken from sheep 8th Sept 2006)
|
Originally isolated in Morocco on BHK cell cultures
(used to infect BHK cells at Pirbright on 18th Feb2008) |
Shelf A9, box A9 β, rows I1-J10
(added to collection on 13th March 2008) |
OV4/5B Foci 2006 |
BHK1
[IAH/BHK1] |
infected BHK cell culture supernatant supplied by Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco |
|
|||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2), and Seg-2 sequence analyses (4) |
Fès, Morocco
(Sample taken from sheep 27th Oct 2006) |
Originally isolated in Morocco on BHK cell cultures
(used to infect BHK cells at Pirbright on 18th Feb 2008) |
Shelf A9, box A9 β, rows G1-H10
(added to collection on 13th March 2008) |
ZA3 Foci 2006
|
BHK1
[IAH/BHK1] |
infected BHK cell culture supernatant supplied by Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco |
|
|||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2), and Seg-2 sequence analyses (4) |
Oujda, Morocco
(Sample taken from sheep 9th Oct 2007) |
Originally isolated in Morocco on BHK cell cultures
(used to infect BHK cells at Pirbright on 18th Feb2008) |
Shelf A9, box A9 β, rows E1-F10
(added to collection on 13th March 2008) |
509/06 Foci 2006 |
BHK1
[IAH/BHK1] |
infected BHK cell culture supernatant supplied by Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco |
|
|||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2), and Seg-2 sequence analyses (4) |
Oujda, Morocco
(Sample taken from sheep 9th Oct 2007) |
Originally isolated in Morocco on BHK cell cultures
(used to infect BHK cells at Pirbright on 18th Feb2008) |
Shelf A9, box A9 β, rows C1-D10
(added to collection on 13th March 2008) |
505/06 Foci 2006 |
BHK1
[IAH/BHK1] |
infected BHK cell culture supernatant supplied by Mehdi El Harrak
(Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco |
|
|||
|
BTV-1
Identified as BTV-1 by type-specific RT-PCR targeting Seg-2 (2), and Seg-2 sequence analyses (4) |
Oujda, Morocco
(Sample taken from sheep 9th Oct 2007) |
EDTA Blood
(isolated in Morocco) |
Shelf A9, box A9 α, rows I1-J10
(added to collection on 12th March 2008) |
Ham Foci 2006 |
BHK1
[IAH/BHK1] |
Tissue culture supernatant supplied by Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco |
|
|||
|
BTV Type 1
(Identified as BTV by Seg-7 Group specific RT-PCR, Typed by Seg-2 RT-PCR & sequence analysis) |
Isolated from a small village called Guenfouda near Oujda in the East of Morocco. |
Isolated from a Sheep showing clinical BT in Morocco, using embyonated chicken eggs and BSR cells |
Shelf D3 Box D3 ALPHA Rows C1-D10
(added to collection on 10th Nov 2006 ) |
1- OV4 - passage 1E3BSR
(A112/06 No 2) |
E1/BSR4 [IAH/BHK2] |
supplied by Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco - arrived 20th Oct 2006)
|
Related to sample MOR2006/06
Derived from
|
|||
| MOR2006/06 |
BTV Type 1
(Identified as BTV by Seg-7 Group specific RT-PCR, Typed by: , Seg-2 RT-PCR) |
Isolated from a
small village called Guenfouda near Oujda in the East of Morocco. (sample taken Oct 2006) |
Isolated from a Sheep showing clinical BT in Morocco, using embyonated chicken eggs and BSR cells |
Shelf D3, box D3α, rows A1- B10
(added to collection on 10th Nov 2006)
|
1- OV4 - passage 1E3BSR
(A112/06 No 1) |
E1/BSR4 [IAH/BHK2] |
supplied by Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco - arrived 20th Oct 2006)
|
Related to sample MOR2006/07 * Duplicate samples, box D3α, position B10(g), supplied to Giovanni Savini, IZS Terramo, Italy - 4th Dec 2006 * Duplicate samples, box D3α- position A1(p) supplied to Carolin Schumacher (Merial Lyon 22nd Nov 2007) * Vial Position A2 was given to Eva Veronessi (Pirbright) on 26th February 2013 * Derived from
[S2: EU625362, S6: EU422953] |
||
| 2005 | ||||||||||
|
BTV-1 |
Australia |
Cow |
Shelf D13, Box D13 β rows E1 to E10 |
Old isolate No.: B478
Sample sent as 'BTV1-21' |
1ECE.1AA, 2BHK21, 1HmLu-1 |
Peter Kirkland, Elizabeth Macarthur Agricultural Institute
|
Note supplied with sample: CPE positive / BT-PCR positive
It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL
(MTA) |
|||
| 2004 | ||||||||||
|
BTV- 1
Virus serotype identified by RT-PCR using type specific primers and sequencing (Seg-2) at SCWDS . |
USA: St. Mary Parish Louisiana
Sample taken in the Fall of 2004 |
White tailed deer A deer was observed by a hunter to be staggering and was killed /tissues collected. Isolated at the Southeastern Cooperative Wildlife Disease Study (SCWDS) Athens GA. |
Shelf A16, box A16 α, rows C1-D10
(Added to collection 16th Oct 2008) |
Virus ID: 05-350924 Tube labelled: 350924: BHK/2 from re-isolation (sample preparation date not recorded) |
Isolated from tissue sample E1/BHK2 |
Virus isolate supplied by / reagent also available from : USDA, Aphis, VS, NVSL, PAS Reagents Office. PO box 844 Ames .IA 50010 USA |
Technical contact in USA: Donna Johnson : Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844 Ames IA, 50010 USA. (82% similar (Seg-2) to other BTV-3 isolates from Florida (1130 bp region)) |
|||
| 2003 | ||||||||||
| AUS2003/02 |
BTV Untyped |
Australia NSW (2003) |
Cow |
Old isolate No.: A903
Sample sent as 'BTV1-21' |
Shelf D13 , Box D13 β rows H1 to H10 |
1ECE.1AA,1BHK21, 1HmLu-1 [IAH/??] |
Peter Kirkland, Elizabeth Macarthur Agricultural Institute
|
Note supplied with sample: CPE positive / BT-PCR positive
It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL
(MTA) |
||
|
BTV-1 Typed by RT-PCR targeting Seg-2 and sequence analysis |
Australia
(2003) |
Cow |
Old isolate No.: A913 Origin: EMAI 03/1123 Sample sent as 'BTV1-21' |
Shelf C14, Box C14 gamma, Rows A1-B10
Added to collection 23 Aug 2011 |
1ECE.1AA,1BHK21, 1HmLu-1 |
Peter Kirkland, Elizabeth Macarthur Agricultural Institute
|
Note supplied with sample: CPE positive / BT-PCR positive
It is uncertain if the date given is sampling date or date of arrival at EMAI. (MTA) |
|||
|
BTV Type 1
(Typed by Seg-2 RT-PCR / sequence analysis) |
India |
From blood |
Shelf D4, box D4 γ, rows I1-J10
(added to collection on 24th Nov 2006) |
|
[+IAH/BHK3 ] |
Sample given to Sushila maan IAH Pirbright Sept 2006 for sequencing studies Distribution restricted by MTA |
||||
|
BTV Type 1
(Typed by SNT & by Seg-6 RT-PCR / sequence analysis)
|
Thoothukudi, Tamil Nadu, India |
Sheep
(Isolated at Tamil Nadu Veterinary And Animal Sciences University, Chennai-600 051 (India)) |
Shelf B5, box B5γ, rows I1- J10
(added to collection on 16/08/2005)
|
(125) |
BHK cells
(BHK5) [IAH/E1/BHK2] |
Dr A. Wilson Aruni |
[S6: AJ783903]
(MTA) |
|||
|
BTV Type 1
(Typed by SNT & by Seg-6 RT-PCR / sequence analysis)
|
Coimbatore, Tamil Nadu, India |
Sheep
(Isolated at Tamil Nadu Veterinary And Animal Sciences University, Chennai-600 051 (India)) |
shelf B5, box B5α rowsA1-B10
(added to collection 8th July 2005) |
(122) |
BHK cells
(BHK5)
[IAH/E1/BHK2] |
Dr A. Wilson Aruni |
Vials position A1 freezer A given to Anna Sanders (IAH-P) on 27th June 2008. Spare vial in this position left in freezer B (MTA)
[S6:AJ783902] |
|||
| 2001 | ||||||||||
| GRE2001/15 | BTV-1 |
Greece 2001 |
Shelf D10,
box D10 beta,
rows C1-D10 |
|||||||
| GRE2001/14 | BTV-1 |
Greece 2001 |
Shelf D10,
box D10 Beta,
rows A1-B10 |
|||||||
| GRE2001/13 | BTV-1 |
Greece 2001 |
Shelf D10,
box D10 alpha,
rows I1-J10 |
|||||||
| GRE2001/12 |
BTV-1 Identified by BTV-1 specific real time PCR (LSI assay) |
Lesbos Agiasos-Achladeri (Central island) GREECE
[39o 05΄Ν 26o 09΄Ε]
(15/Oct/2001) |
Isolated from Sheep (spleen) |
Shelf B18,
box B18
β
,
rows I1-J10
(Added to
collection 5th Aug 2010) |
GR 1472/01 LS | From spleen [IAH/KC1] |
Kiki Nomikou
Centre of Athens Vet. Institutions, Virus Lab, Athens & IAH |
Isolated from the same location (same farm) but different animal as Distribution restricted by MTA |
||
|
BTV Type 1
(identified as BTV by ELISA and Seg-7 RT-PCR [3] : Typed by serum neutralisation assay and seg 2 RT-PCR [2]) |
GREECE , Karies-Zagoriou-Miliotades Ioannina (NW)
(sample taken 10th Sept2001) |
Sheep (blood)
isolated at: Centre of Athens Vet institutions, virus lab, by K.Nomikou
|
Shelf A12, box A12 α, rows I1-J10
(Added to collection 18th July 2008
|
animal code 1 |
Blood
adaptedto BHK 21 CELLS
BHK 9 [+IAH/BHK1]
|
Nomikou Kiki
Centre of Athens Vet. Institutions, Virus Lab, Athens
|
|
|||
|
BTV-1
(identified as BTV by ELISA and Seg-7 RT-PCR: Typed by serum neutralisation assay & Seg 2 RT-PCR) |
GREECE , Thesprotia village: Potamia-Paraminthia
(west-mainland Greece)
( sample taken 16th Nov 2001)
Vector insects in the area C.obsoletus |
Sheep (spleen - postmortem) At: Centre of Athens Vet institutions, virus lab K.Nomikou and O. Mangan
isolated 3rd Dec2001 |
Shelf A12, box A12 α, rows E1-F10
Added to collection on 14th July 2008 |
|
from spleen, to BHK 21 CELLS
E1 BHK 6 [+IAH/BHK1]
|
Kiki Nomikou
Centre of Athens Vet. Institutions, Virus Lab, Athens
Sequenced at E1 BHK7 |
[ Seg-1 KP820887; Seg-2 KP821007; Seg-3 KP821129; Seg-4 KP821249; Seg-5 KP821369; Seg-6 KP821489; Seg-7 KP821611; Seg-8 KP821731; Seg- 9 KP821852; Seg-10 KP821972; |
|||
|
BTV Type 1
(identified as BTV by ELISA and Seg-7 RT-PCR : Typed by serum neutralisation assay & seg 2 RT-PCR) |
GREECE , Lesbos Agiasos-Vasilika, Central island
Animal code 295
(sample taken 17th Oct 2001) |
Sheep (BLOOD) Isolated on 24th Nov 2001
at the Centre of Athens Vet institutions, virus lab by K. Nomikou |
Shelf A12 Box A12 α, rows C1-D10
Added to collection on 14th July 2008
|
|
from blood to BHK 21 CELLS
BHK 6 [+IAH/BHK1] |
Nomikou Kiki
Centre of Athens Vet. Institutions, Virus Lab, Athens |
|
|||
|
BTV Type 1
(identified as BTV by ELISA and Seg-7 RT-PCR : Typed by serum neutralisation assay and seg 2 RT-PCR) |
Greece, Lesbos Agiasos-Achladeri, Central island
(sample taken 15th Oct 2001) |
isolated from Sheep (spleen)
Isolated at the Centre of Athens Vet institutions, virus lab, by: K. Nomikou
(Isolated on 1st Nov2001) |
Shelf A11 Box A11 γ, rows I1-J10
(Added to collection 10th July 2008 |
Animal code 280 |
Spleen to BHK 21 CELLS
BHK 3 [+IAH/BHK1]
|
Supplied by Nomikou Kiki, Centre of Athens Vet. Institutions, Virus Lab, Athens
|
|
|||
|
BTV Type 1
(Identified as BTV by ELISA & RT-PCR,
(Typed by SNT & by Seg-2 RT-PCR) |
Lesbos Agiasos-Achladeri (Central island) GREECE
[39o 05΄Ν 26o 09΄Ε]
(15/Oct/2001) |
From sheep (animal code 265)
(Isolated at: Centre of Athens Vet. Institutions, Virus Lab, Athens by: Dr. Nomikou Kiki 1-11-200 ) |
shelf B3, box B3α rows C1-D10 & shelf B5, box B5α rows I1-J10
sample received 19th Oct. 2004 |
GR1472/01 LS (265) |
BHK 21 cells
from blood
BHK2
[IAH/E1/BHK2]
|
Supplied by: Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens |
Seg 10 sequenced at E1/BHK5
Isolate used by Shujing Rao, IAH Pirbright for cDNA synthesis, to send to P.Roy for VLP production (March 2005)
Aliquots 1/10a & b (p) given to Eva Veronesi (IAH Pirbright) 27th Sept 2005
Isolated from the same location (same farm) but different animal as GRE2001/12
[S3:DQ186807; S5: AM778438 S10:AY677628] |
|||
|
BTV Type 1
Identified as BTV by ELISA & RT-PCR, Typed by serum neutralisation assay |
Smiksi, Grevena, Greece, (NW)
[40o 04΄ Ν, 21o 07΄Ε]
(31/Dec/2001)
Vector insects in the area C.obsoletus |
Isolated from seep spleen (post-mortem)
At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana |
Shelf B4, box B4 alpha, rows I1-J10
30th Sept. 2004 |
GR15/01GRES |
BHK 21 cells
BHK8
[IAH/BHK2] |
provided by Dr. K. Nomikou, Centre of Athens Vet. Institutions, Virus Lab, Athens.
Vial position I1 Freezer A&B given to K.Nomikou (TPI) for sequencing 30th Nov 2015
Sequenced at BHK10
|
[Seg3:DQ186820 Seg10:AY449657]Seg-10 sequenced at BHK10]
[Seg-1 KP820886; Seg-2 KP821006; Seg-3 KP821128; Seg-4 KP821248 Seg-5 KP821368 Seg-6 KP821488; Seg-7 KP821610; Seg-8 KP821730; Seg- 9 KP821851; Seg-10 KP821971; |
|||
|
BTV Type 1
Identified as BTV by ELISA & RT-PCR, Typed by serum neutralisation assay |
Oraiokastro - Delvinaki, Ioannina, Greece (N.W)
[39o 59΄Ν, 20o 33΄Ε]
(18/ Sept/ 2001 |
Sheep (spleen)
At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana |
Shelf B4, Box B4β Row A1- B10
Added to collection on 30th Sept. 2004 |
GR1245/01 IOS |
BHK 21 cells
Spleen
(E1BHK9) [IAH/BHK2] |
Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens. |
Vials position A1 freezer A given to Anna Sanders (IAH-P) on 27th June 2008. Spare vial in this position left in freezer B
[Seg3:DQ186822] |
|||
|
BTV Type 1
(Identified as BTV by: ELISA , SNT & RT-PCR. Serotyped by: SNT) |
Greece Lesbos Agiasos-Achladeri (Central island) Animal code:280
[39o 05΄Ν, 26o 09΄Ε]
(15/Oct/2001) |
From sheep (01/Nov/2001 At:Centre of Athens Vet. Institutions, Virus Lab. By: Dr. Nomikou Kiki) |
Shelf A2, Box A2γ, RowsG1-H10
(20/May/2003) |
GR1472(2)/01 LS (280) |
spleen
BHK 21 cells
( BHK3 ) [IAH-BHK2]
|
Dr. Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab. Athens |
Isolated from the same location (same farm) as GRE2001/03 (GR1472(1)/01LS)
Tubes G1 & H1 (from freezer A & B) supplied to S. Anthony & H. Jones, Pirbright 7th June 2004 |
|||
|
BTV Type 1
(Identified as BTV by: ELISA , SNT & RT-PCR. Serotyped by: SNT) |
Greece Lesbos Agiasos-Achladeri (Central island) Animal code:280
[39o 05΄Ν, 26o 09΄Ε]
(15/Oct/2001) |
From sheep (01/Nov/2001 At:Centre of Athens Vet. Institutions, Virus Lab. By: Dr. Nomikou Kiki) |
Shelf A2, Box A2γ, Rows I1-J10
(20/May/2003) |
GR1472(1)/01LS (280) |
spleen
BHK 21 cells
( BHK3 ) [IAH-BHK2] |
Dr. Nomikou Kiki Centre of Athens Vet. Institutions , Virus Lab. Athens |
Isolated from the same location (same farm) as GRE2001/04 (GR1472(2)/01LS)
Tubes I1 & J1 (freezer A & B) supplied to S. Anthony & H. Jones, IAH 7th June 2004
|
|||
|
BTV-1
(Typed by: SNT & Seg-2 RT-PCR plus sequencing) |
Greece Lesbos
[39o 05΄Ν, 26o 09΄Ε]
(2001) |
Sheep
21/12/2001 |
Shelf A5, Box A5γ, positions G1-H10 |
GR-1472/01 LS |
spleen
BHK 21 cells
[IAH-BHK3] |
Dr. Charalabos Billinis Aristotle University Thessaloniki
[R22/01 A spleen] |
[S10: ] |
|||
|
|
BTV Type 1 (eastern topotype)
(Typed by: SNT & Seg-2 RT-PCR plus sequencing) |
Greece, Agiasos-Achladeri-Lesvos
[39o 05΄Ν, 26o 09΄Ε]
(2001)
Vector insects in the area C.imicola |
Isolated from a sheep spleen post-mortem
26/12/2001 |
Shelf A5, Box A5γ, positions E1-F10
(01/Apr/2004) |
GR-1472/01 LS |
BHK 21 cells
[IAH-BHK3] |
Dr. Charalabos Billinis Aristotle University Thessaloniki
[R22/01 E Blood]
Sequenced at BHK5 |
Tubes F1(freezer A&B) given to Adam Meyer (Pirbright) for RNA production for Raniero Lorenzetti (June 2005) * Sample E1 (freezer A) given to Anna Swain Pirbright on 16th May 2008, spare sample (from freezer B ) stored as Misc GRE2001/01 * Vial E2 (freezer A) to Eva Veronesi (Pirbright 6th Nov 2009), spare tube from freezer B was retained in the 'miscellaneous' box D18α, position H1 at the bottom of freezer A * Tube E3 (freezer A) given to Eva Veronesi (Pirbright on 13th May 2010) *
Tube E3 freezer B to Elizabeth Morecroft (Pirbright) 9th July 2013.
* [S2, AJ585121 S6:AJ586664, S9: EU220289]
[Seg-1 KP820885; Seg-2 KP821005; Seg-3 KP821127; Seg-4 KP821247; Seg-5 KP821367; Seg-6 KP821487; Seg-7 KP821609; Seg-8 KP821729; Seg- 9 KP821850; Seg-10 KP821970; |
||
|
(41002A82) |
BTV Type 1 (Type confirmed by Seg-2 RT-PCR and sequencing) |
India (Chennai.) (19/Feb/2001) |
Culicoides |
Shelf B2, boxB2α, rows I1-J10
(16/Mar/2005)
|
(INDe ) |
BHK cells
(BHK6) [IAH-BHK3] |
Dr. Gaya Prasad College of Veterinary SciencesCCS, Haryana Agricultural University, Hisar, India |
Sample Nos. 1/10g(a+b) were given to Natalie Ross-Smith (IAH Pirbright) for sequencing studies, 7th March 2006 * Duplicate samples I2(g) supplied on 19th Dec 2007, to JongChul, Jeong, Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea * Vial positions I 3 (g) were given to Eva Veronesi (IAH Pirbright on 13th May 2010 for Ph.D studies). One vial was retained in the Misc box C Freezer A position E2 * Vial removed from Position E2, Misc Box C, Freezer A.and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013. (No corresponding box/vial in Freezer B)
[ Seg3:DQ186811 Seg6:AJ586663] * Distribution restricted by MTA |
||
| 1999 | ||||||||||
|
(41002921) |
BTV Type 1 (Type confirmed by Seg-2 RT-PCR and sequencing) |
India (Haryana.)
(06/Aug/1999) |
Sheep |
Shelf B3, box B3β, rows A1-B10 |
(INDd ) |
BHK cells
(E2/BHK9) + [IAH/BHK1] |
Dr. Gaya Prasad College of Veterinary SciencesCCS, Haryana Agricultural University, Hisar, India |
[S2: S6:AJ586662 S7:AM261977]
(MTA)
|
||
| 1996 | ||||||||||
| AUS1996/03 |
Typed as BTV-1 by RT-PCR (targeting Seg-2) and sequencing |
Australia (1996) |
Cow |
Shelf C14, Box C14 beta, Rows I1-J10 |
Old isolate No.: Z66
Sample sent as 'BTV1-21' |
1ECE/1AA/1BHK21/ 1HmLu-1 [IAH/??] |
Peter Kirkland, Elizabeth Macarthur Agricultural Institute
|
Note supplied with sample: CPE positive / BT-PCR positive
It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL
(MTA) |
||
| 1997 | ||||||||||
| AUS1997/01 |
BTV |
Australia (1997) |
Cow |
Shelf D13 , Box D13 β rows G1 toG10 |
Old isolate No.: Z169
Sample sent as 'BTV1-21' |
1ECE/1AA/1BHK21/ 1HmLu-1 [IAH/??] |
Peter Kirkland, Elizabeth Macarthur Agricultural Institute
|
Note supplied with sample: CPE positive / BT-PCR positive
It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL
(MTA) |
||
| 1992 | ||||||||||
|
(Eastern BTV-1 reference strain)
(410025A0) |
BTV Type 1
(Type confirmed by Seg-2 RT-PCR and sequencing) |
India
(03/Nov/1992)
|
NK |
Shelf C1, box C1α, rows G1-H10 Added to collection 14th Sept 2005 |
(INDB) |
BHK cells
E1/BHK10 [IAH-BHK2] |
Duplicate vials from G10(g) sent to Monsterrat Augero, LCV, Spain under an MTA 26th July 2010
for western topotype BTV-1 reference strain see RSArrrr/01
|
Reference strain BTV-1[E]
[ Seg-1: KP696526 ; Seg-2: AJ585112 ; Seg-3: KP696527; Seg-4 : KP696528; Seg-5: KP696529; Seg-6: KP696530; Seg-7: KP696532; Seg-8: KP696532; Seg-9: KP696533; Seg-10: KP696534;
[Seg-2: AJ585111, Seg-6:AJ586660 ]
(MTA)
|
||
|
(4100244C) |
BTV Type 1(e) identified as BTV by immuno-fluorescence, and immunoperoxidase tests
(Type confirmed by serology and Seg-2 RT-PCR and sequencing) |
Avikanagar-Rajasthan, India
(Isolate from sample taken in Avikanagar in Rajasthan State, India 3rd March 1992) (dominant vector C.imicola)
|
Isolated from blood of sheep showing typical bluetongue clinical signs
In India by Dr. Gaya Prasad Hisar |
|
IND01-01 (INDA)
|
BHK [IAH/BHK] |
Dr. Gaya Prasad College of Veterinary SciencesCCS, Haryana Agricultural University, Hisar, India
Local vector -C.imicola |
Vial position G10 were removed from Freezer A & B.
[Seg-6:AJ586659AJ586659 Seg-7: AM261976AM261976]
Distribution restricted by MTA
[ Seg-1 JQ282770; Seg-2 AJ585111; Seg-3 JQ282771; Seg-4 JQ282772; Seg-5 JQ282773; Seg-6 AJ586659; Seg-7 JQ282774; Seg-8 JQ282775; Seg- 9 JQ282776; Seg-10 JQ282777; |
||
| 1988 | ||||||||||
|
(4100274F) |
BTV Type 1 (Type confirmed by Seg-2 RT-PCR and sequencing) |
India
(24/Sept/1988) |
NK |
Shelf C1, box C1α, rows E1-F10 Added to collection 14th Sept 2005 |
(INDC ) |
BHK cells
BHK9 [IAH-BHK2] |
|
[S2: S6:AJ586661]
Distribution restricted by MTA
* Vial position E1(g) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013. Corresponding vial in Freezer B has not been removed. *
|
||
| 1987 | ||||||||||
|
BTV Type 1
(Type confirmed by Seg-2 RT-PCR and sequencing) |
Kuala Lumpur (00/Apr1987) |
NK |
Shelf B5, box B5γ, rows C1-D10
(added to collection on 16/08/2005) |
( ) |
BHK cells
(E1/BHK2) [IAH/BHK3] |
|
Duplicate samples C1(g) supplied on 19th Dec 2007, to JongChul, Jeong, Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea [Seg2: Seg3:DQ186816 ]
Distribution restricted by MTA * Vial position D1(p) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013. Corresponding vial in Freezer B has not been removed. * |
|||
|
|
BTV Type 1
(Type confirmed by Seg-2 RT-PCR and sequencing) |
Sudan (16/Apr/1987) |
NK |
Shelf C3, box C3β, rows E1 -F10
(added to collection on 18th Nov 2005) |
(NY-76 ) |
BHK cells
E2/BHK2/PP1 [IAH/BHK2] |
|
[S2: S6:AJ586658]
|
||
| 1985 | ||||||||||
|
BTV-1 |
India
Sample taken in 1985 |
Shelf D5 Box D5 alpha Rows E1-F10 (added to collection on 28th Nov 2006) |
|
[IAH/BHK3] |
Distribution restricted by MTA | |||||
| 1983 | ||||||||||
| SUD1983/04 | BTV-1 |
Shambat, Sudan
Sample taken in 1983 |
Isolated from bovine blood (Sentinel herd) |
Shelf B11, box B11γ, rows G1-H10 added to collection 22nd Oct 209 |
NY76 |
BHK cells
E2/BHK2/PP1 [IAH/BHK3 |
M.E.H. Mohammed |
Note: Sample was originally supplied as untyped EHDV. However, subsequent work has identified this as an isolate of BTV-1 * Vial position G2(g) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 22 May 2013. Corresponding vial in Freezer B has not been removed. |
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| 1982 | ||||||||||
|
(40FED549) |
BTV Type 1
(Type confirmed by Seg-2 RT-PCR and sequencing) |
Nigeria (19/Aug/1982) |
NK |
shelf B1, box B1α rows E1-F10
(02/Feb/2005) |
(N-423 ) |
BHK cells
(E1/BHK3) [IAH: BHK1] |
|
* Vial position E1(g) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013. Corresponding vial in Freezer B has not been removed. *
[Seg2:
(MTA) |
||
|
BTV Type 1
(Type confirmed by Seg-2 RT-PCR and sequencing) |
Cameroon (29/Oct/1982) |
Sheep |
shelf C2, box C2α, rows G1-H10 Added to collection on 18th Oct 2005 |
(82/4) |
BHK cells
(E1/BHK1) [IAH/BHK2] |
|
* Vial position G1(g) Freezer A given to Elizabeth Morecroft (Pirbright) 9th Jul 2013. Corresponding vial in Freezer B was not removed. * [ Seg-1 KP820878; Seg-2 KP820998; Seg-3 KP821120; Seg-4 KP821240; Seg-5 KP821360; Seg-6 KP821480; Seg-7 KP821602; Seg-8 KP821722; Seg- 9 KP821843; Seg-10 KP821963; |
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| 1981 | ||||||||||
|
BTV Type 1/6? |
Australia (5/Oct/1981) |
|
|
( CSIRO 154 No. 34980) |
Vero, BHK cells
(V3/BHK7) |
|
|
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|
BTV Type 1/6? |
Australia (7/Oct/1981)) |
NK |
|
( CSIRO 156 No. 34998) |
Vero, BHK cells
(V4/BHK7) |
|
|
|
||
|
(41002D85) |
BTV Type 1 |
Australia (7/Oct/1981) |
NK |
Shelf C18
Box C18β
Rows
A1-B10
(Added to collection on 30th May 2012) |
( CSIRO 156 No. 34993) |
Vero, BHK cells
(V4/BHK7) [IAH/KC2] |
|
|
[S2: S6 AJ631214 ] |
|
| 1979 | ||||||||||
| AUS1979/10 |
BTV-1 |
Australia (1979) |
?? |
Old isolate No.: W120 |
??/2BHK21 (PP) [IAH/??] |
Peter Kirkland, Elizabeth Macarthur Agricultural Institute
|
Note supplied with sample: CPE positive / BT-PCR positive
It is uncertain if the date given is sampling date or date of arrival at EMAI. Origin: ex LPL
(MTA) |
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|
BTV type 1 |
Australia, Northern Territories 1979 |
(T. St George) |
shelf B1, box B1α rows I1 - J10
(02/Feb/2005) |
(CS156 7906-26-0004) |
(BHK3/V4/ BHK1/V1)
[IAH: BHK1]
|
Dr. Ian Pritchard AAHL Geelong, Australia |
Aliquots 1/10a & b (p) given to Eva Veronesi (IAH Pirbright) 27th Sept 2005 (MTA) |
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| unknown sampling date | ||||||||||
|
BTV Type 1
(Type confirmed by Seg-2 RT-PCR and sequencing) |
Australia |
NK |
Shelf C18
Box C18β
Rows
C1-D10
(Added to collection on 30th May 2012) |
|
BHK cells |
|
[S2:] (MTA*) * Vial position C1(g) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013. Corresponding vial in Freezer B has not been removed. * |
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*IAH dsRNA virus collection number: [Country code, year / sample number] rrrr = reference strain vvvv = vaccine strain]