ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 12 (BTV-12)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection Contents

 

 

 Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus (BTV) serotype 12

 *IAH dsRNA virus collection number

 

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 

 (identification method)

Place of original sample eg country town

 [grid reference]

 

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 

 Adapted to which cell types 

 

(Passage history of sample)

 

[IAH passage history]

* Original sample supplied to the collection by person / institution

 

[IAH, ISIS sample number]

Comments

 

 References &  accession numbers  

RSArrrr/12

BTV-12

 

(western topotype - reference strain)

Supplied by OVI S. Africa* (20/10/83)

NK

Shelf B4,

Box B4γ.

Rows G1 -H10

 

 Added to collection on 16th June 2005

RSArrrr/12

 (Ref. Strain) 

E1/BHK6 

+

[IAH/BHK1]

*

Duplicate samples  G1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

Vial H1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial H2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

*  

Vial G sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

*  

Vial positions H4(P) taken out of freezer A, and given to Lizzie Morecroft (IAH) on 26th June 2012,  corresponding vial in freezer B has  not been removed

 

[Seg-2: KP821100,

Seg-6: KP821583, 

Nomikou et al. 2015 (1)]

 

 

[Seg-2: AJ585133,

Seg-6: AJ586711,

  Maan et al 2007 (2)]

ISR2010/36

BTV

Type 12

Israel  

Shelf C17,

Box C17 Gamma.

Rows A1 -B10

       
ISR2010/35

BTV

Type 12

Israel  

Shelf C17,

Box C17 Beta.

Rows I1 -J10

       
ISR2010/27

BTV-12

 

Identified as BTV-12 by Seg-2 specific

 Real time RT-PCR  

Israel

Ashdod Yaakov

 10th
November 2010
Isolated from cattle
 blood at IAH-P
Shelf C15, 
box C15γ,
rows E1-F10
 
Added 4/10/11  

2993

KVI Id 88958
[KC1 at IAH-P]

Jacob Brenner, Kimron Institute Israel

 

 Samples arrived 30th August 2011

 

IAH Stars number
 A59/11 03
 
ISR2010/26

BTV-12

Identified as BTV-12 by Seg-2 specific Real time RT-PCR 

Israel
Bustan Hagalil

 5th October 2010
Isolated from cattle
 blood at IAH-P
Shelf C15, 
box C15γ,
rows C1-D10
 
Added to reference 
collection on 4/10/11

2688

KVI Id 85533
 [KC1 at IAH-P]

Jacob Brenner, Kimron Institute Israel  

Samples arrived 30th August 2011

 

IAH Stars number
A59/11 02
 

ZIM2003/04

BTV

type 12

 

identified by RT-PCR

Zimbabwe (Lone Oak) 2003

Culicoides imicola

Shelf B6, 
box B6γ,
rows G1-H10
 
Added to 
collection 
7th Sept 2005

 

E1/BHK1

[IAH-BHK2]

[R06/03,No.82]

 

(supplied 25/08/03)

 

  contents

BRA2002/01 BTV-12

Typed by serum neutralisation assay

Brazil  

Shelf E1,

 box E1 β,

 rows C1 to D10

 

(Added to collection on 26th June 2007)

    believed to be from the outbreak in Brazil 2001-2002

Duplicate samples from row 'C1g' given to Tamara Jabbar  IAH-Pirbright 10th March 2008 

OIE archive  report page 105

NIG1982/09

 

 BTV Type 12

(by sequence analysis of Seg 2)

Nigeria (12/09/82)

NK

Shelf C3, 
box C3α,
rows E1 - F10
 
(added to 
collection on 
15th Nov 2006)

N-396

E1/BHK3  

[IAH/BHK2]

 

 

 

KEN----/02

BTV

Type 12

Kenya

NK

Shelf D1, 
box D1β,
rows I1 - J10
 
(Added to 
collection on 
7th Aug 2006)

 F.D in ampule

 

P1

 

 

[IAH/BHK1]

 

*

Vial position I1(g) removed from Freezer A and given to Elizabeth Morecroft (Pirbright) 9th Jul 2013.  Corresponding vial in Freezer B has not been removed.

*

 

[S2:

S6:AJ586712]

References
1.     
 

Nomikou K, Hughes J, Wash R, Kellam P, Breard E, Zientara S, Palmarini M, Biek R, Mertens P. (2015) Widespread Reassortment Shapes the Evolution and Epidemiology of Bluetongue Virus following European Invasion. PLoS Pathog. 2015 Aug 7;11(8):e1005056. doi: 10.1371/journal.ppat.1005056. eCollection 2015.

   
2.

Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC (2007) Analysis and phylogenetic comparisons of full-length VP2 genes  of the 24 bluetongue virus serotypes.

 J Gen Virol. 88, 621-630.

   

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