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Isolates of
Bluetongue virus type 14
(BTV-14)
in the dsRNA virus collection at IAH Pirbright
Return to Virus Collection »
Contents
|
Family:
Reoviridae, Genus: Orbivirus, Species:
Bluetongue virus
serotype 14
(BTV-14) |
|
Virus
Species
(identification
method) |
Serotype
(identification
method) |
Place of
original sample
eg
country town
[grid
reference]
(date
original sample was taken)
|
Isolated from which host species
e.g
cow, sheep, etc
(Date
of virus isolation, where, by whom) |
Position
of storage in reference freezers
(Date
added to collection)
|
IAH
dsRNA virus collection number: [Country code year / sample number]
(Suppliers
Isolate Designation)
|
Adapted
to which cell types
Original
sample material
(Passage
history of sample) |
*
Original sample
supplied to the collection by
person
/ institution
[IAH
virus diagnosis, ISIS sample number]
Comments |
References
&
accession
numbers |
|
RSArrrr/14 |
BTV-14
(western topotype
-
reference strain) |
Supplied
by OVI
S. Africa*
(13/04/83) |
NK
|
shelf C2,
box C2γ,
rows A1-B10
Added to
collection on
4th Nov 2005
|
(Ref. Strain)
|
E1/BHK7
[IAH/BHK4]
|
*
Vial
A1(g) supplied on 19th Dec 2007, to JongChul, JeongJa Yeon Agrotech Co.,
Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330,
Korea
*
Vial B1 (p) A&B sent to Mehdi El Harrak,
Biopharma , Morocco 29th June 2009
*
Vial B2 sent to Denis Kolbasov,
Russian National Institute of Veterinary Virology 29th Sept 2009
*
Vial A sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March
2011
*
Vial
positions A5 (G) taken out of freezer A, and given to Lizzie Morecroft (IAH)
on 26th June 2012, corresponding vial in freezer B has not been removed
*
|
[Seg-1:
KP820863,
Seg-2:
KP820983,
Seg-3:
KP821105,
Seg-4:
KP821225,
Seg-5:
KP821345,
Seg-6:
KP821465,
Seg-7:
KP821587,
Seg-8:
KP821707,
Seg-9:
KP821827,
Seg-10:
KP821948,
Nomikou
et al 2015 (1)]
[Seg-2:
AJ585135,
Seg-6:
AJ586714]
|
|
SPA2012/01 |
BTV-14
Identified as
BTV-14 by Seg-2 specific
Real time
RT-PCR
|
Calf exported from
Lithuania to Spain in August 2012 |
Isolated from a cow
imported
from Lithuania |
Shelf D7
Box D7 alpha
Rows I1
–J10 Added 04/02/13
|
Original blood
LCV 2012/003523 |
E1/BHK1/V1
[V2 at PIR] |
Marta Vigo Martin
LCV Algete Spain
A163/12 02
Received 22/11/12
note only 9 vials of
glycerol in Freezer B |
|
|
POL2012/02 |
BTV-14 Identified as BTV-14
by Seg-2 specific
Real time RT-PCR
|
Sokółka poviat (powiat Sokółka) end Sept 2011
|
Cattle blood
No clinical signs
|
Shelf D8,
box
D8 α,
rows E1-F10
Added to collection
15th Mar 2013
|
|
KC4
at Pirbright Institute
|
Jan Zmudsinski
National veterinary
research institute,
Pulawy, Poland
A160/12 04
Received 21/11/2012
|
Isolate derived from
POL2012/01
vial E1 was
removed from freezer A ; vial E10 was removed from freezer B
|
|
BTV-14 Identified as BTV-14
by Seg-2 specific
Real time RT-PCR
|
Sokółka poviat (powiat Sokółka) end Sept 2011
|
Cattle blood
No clinical signs
|
Shelf D8,
box
D8 α,
rows C1-D10
Added to collection
15th Mar 2013
|
|
KC3 /BHK1
at Pirbright Institute
|
Jan Zmudsinski
National veterinary
research institute,
Pulawy, Poland
A160/12 04
Received 21/11/2012
|
Isolate derived from
POL2012/01
*
Vial position C1 was taken out of Freezer A an given to
Kyriaki Nomikou (Pirbright) on 14 May 2013 for sequencing
studies . The corresponding vial in
freezer B has not been removed
|
*
Vial position
E1 was
taken out of Freezer A
and given to Kyriaki Nomikou
(Pirbright)
12th June 2013
(Corresponding vial in
freezer B
has not been removed)
|
|
|
POL2012/01
|
BTV-14
Identified as BTV-14 by Seg-2 specific Real time RT-PCR
|
Sokółka poviat (powiat Sokółka)
end Sept 2011
|
Cattle blood
No clinical signs
|
Shelf D7,
Box D7 γRow A1-A10 (G)
and
B1- B7 (P) in freezer A and Rows A1-A9 (G)
and B1- B6 (P) in freezer B
(Added
to collection
2nd Jan 2013)
|
|
[TPI/KC3]
13/12/12
|
Jan Zmudsinski
National veterinary
research institute,
Pulawy, Poland
[A160/12 04]
Received 21st Nov 2012
Isolate used to generate
POL2012/02
|
|
|
RUS2011/01 |
BTV-14 Identified as BTV-14
by Seg-2 specific
Real time RT-PCR
|
Smolensk Galloway village
Tsineevo, Veshkovsk rural settlement, Ugra area
Sept 2011
|
Pool of 5 bovine blood SAMPLES from BTV positive
animals September 2011 (cows imported Cow was imported from Jurgen Greiner
Ginsterweg 1 24576 Hagen Germany, and infected in Russia) |
Shelf D7
Box D7 β
Rows G and H
(added to collection
24th Aug 2012)
|
04
Blood pool animals
75981, 94835, 10055, 01051, 01244 |
Original egg isolation
performed in Nov 2011.
E2, Vero 3, Kidney of Saiga 2
[V1 at IAH-P] |
supplied by
Denis Kolbasov
Russian National
institute of Veterinary
Virology and
Microbiology
Pokrov Vladimir region
Russia 601120
[A88/12 04]
Received 23rd July 2012 |
|
USA2003/08
|
BTV-14
(serotyped by Seg-2 RT-PCR and sequence analyses)
|
Marion County FL, USA (sample taken October 2003)
|
From sheep
clinical history: recumbent, anorexia, transient fever, lip swelling.
(isolate preparation date 25th Aug 2006) |
Shelf E6,
box E6 α,
rows G1 - H10
(added to collection on 15th June 2007)
|
04-279313-2
(tube labelled: 279313-2;
IVD 8/18, 19 B/1; 8-25-06) |
From blood
E1/BHK2/E1/BHK1[+IAH/ BHK4]
|
Virus isolate supplied by : USDA, Aphis, VS, NVSL, PAS
Reagents Office. PO box 844 Ames .IA 50010 USA
nvsluf@aphis.usda.gov
Derived from USA2003/03
Repeated passage of original isolate generated virus of
low titre, so the original isolate was inoculated into ECE in July / August
2006, followed by one pass in BHK-21 cells. original typing attempts as one of
previous US types were unsuccessful
Technical contact in USA: Donna Johnson Equine and Ovine Viruses Section,
Diagnostic virus Lab, PO box 844 Ames IA, 50010 USA.
|
[S2: ]
|
|
USA2003/03 |
BTV-14
(serotyped
by Seg-2
RT-PCR and
sequence analyses) |
Marion County
FL, USA
(sample taken
October 2003)
|
From sheep
clinical
history: recumbent, anorexia, transient fever, lip swelling.
(isolate preparation
date 25th Aug 2006) |
Shelf E1,
Box E1 α,
rows G1 to H10
(added to
collection on 11th May 2007) |
04-279313-2
(tube
labelled: 279313-2;
IVD 8/18, 19 B/1;
8-25-06) |
From blood
E1/BHK2/E1/BHK1
[+IAH/ BHK3] |
Virus isolate
supplied by :
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office. PO box 844 Ames .IA 50010 USA
nvsluf@aphis.usda.gov
Used to generate
USA2003/08
*
Technical
contact in USA: Donna Johnson, Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844, Ames IA, 50010
USA.
repeated
passage of original isolate generated virus of low titre, so the
original isolate was inoculated into ECE in July / August 2006, followed
by one pass in BHK-21 cells. original typing attempts as one of previous
US types were unsuccessful.
|
[S2: ]
|
|
BZE1990/02 |
Identified as
BTV-14
by SNT in 1990
However, type
specific RT-PCR in March 2008 failed to confirm
serotype 14 |
Belize:
Orange Walk
Sample taken
11th Sept 1990 |
Water buffalo blood
Isolated in
1990 |
Shelf A15,
box A15 α,
rows I1-J10
(Added to
collection
16th Oct 2008)
|
Virus ID:
90-46047-27
Tube labelled:
90-46047-27; IVD
BHK/3-CPAE/1-BHK2; 3-12-08 |
E1/BHK3 |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
Reagent also
available from:
USDA, Aphis,
VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA
nvsluf@aphis.usda.go
|
|
|
CAR1982/04 |
BTV Type 14
|
Cameroon
(14/01/82)
|
NK |
shelf
C2,
box C2β,
rows C1-D10
Added to collection on 25th Oct
2005 |
83/1; 81/36 |
E2/BHK1
[IAH/BHK2] |
|
[Seg-2: ,
Seg-6:AJ586715
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