ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 22 (BTV-22)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection Contents

 

 

 Family: ReoviridaeGenus: Orbivirus,  Species:   Bluetongue virus serotype 22 (BTV-22)

Virus Species

 

(identification method)

Serotype

 

(identification method)

Place of original sample

eg country town [grid reference]

(date original sample was taken)

Isolated from which host species e.g cow,  sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

(Date added to collection)

IAH dsRNA virus collection number: [Country code year / sample number]

 

(Suppliers Isolate Designation)

Adapted to which cell types

 

Original sample material

 

(Passage history of sample)

* Original sample supplied to the collection by

person / institution

 

[IAH, ISIS sample number]

Comments,

References

&

 accession numbers

RSArrrr/22

 

BTV-22

 

(western topotype reference strain)

Supplied by OVI

S. Africa*

 

(09/10/87)

NK

 
Shelf B6, 
box B6γ,
rows C1-D10
 
Added to 
collection 
7th Sept 2005

(Ref. Strain)

 

 

Adapted to BHK cells

 

E2/BHK1/

CER1/BHK3

 

[IAH-BHK1]

*

Duplicate samples C1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea 

Vial D1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial D2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

Vial C sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

* 

 

[Seg-2: AJ585143,

Seg-6: AJ586725,

Maan et al 2004 (1),

Singh et al 2004 (3),

Maan et al 2007 (2)]

 

 

contents

MAR2005/05

BTV-22

Martinique (France)

(Sample taken Dec  2005)
from a
non-vaccinated bovine
Shelf C8,
 Box C8α
 Rows G1-H10  

(added to collection 25/08/2010)

 tube labelled:

BTV-22/06.01 - 21
[IAH/BHK1 ] Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France

23rd July 2010
Distribution restricted by MTA

[S2: ]

USA2002/02

BTV-22

 

(serotyped

by Seg-2 

RT-PCR

and

serquence analysis)

Okeechobee County FL, USA

 

(sample taken

30th Oct 2002)

From cattle

 

Sample submitted for export testing, no clinical history given

 

(isolate preparation date 7th Aug 2006)

  Shelf E1,
Box E1 alpha,
rows I1 - J10

 

(added to collection on 15th June 2007)

03-220082-5

 

(tube labelled: 220082-5;

IVD BHK/1;

8-7-06)

 

From blood

 

E1/BHK1/E1/BHK1

[IAH/ BHK4]

virus isolate supplied by : 

USDA, Aphis, VS, NVSL, PAS  Reagents Office.

PO box 844 Ames .IA 50010 USA

nvsluf@aphis.usda.gov

 

Technical contact in USA: Donna Johnson

Equine and Ovine Viruses Section,  Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

repeated passage of original isolate

generated virus of low  titre, so the original isolate was inoculated

into ECE in July 2006,

followed by one pass in BHK-21 cells.

original typing attempts

as one of previous US types were unsuccessful.  Multiple other serotypes were isolated from this herd (BTV-3, 10

and two isolates of unknown serotype - see USA2002/03). 

[Seg-2: ]

TAT1990/01

Initial attempts to identify serotype by SNT were unsuccessful in 1990

 

However, type specific RT-PCR  and sequencing (Seg-2)  March 2008 identified type 22

[1]

Trinidad:

St. Andrews

 

Sample taken 4th Sept  1990

Water buffalo blood

 

Isolated in 1990

Shelf A14,

box A14 γ,

rows I1-J10

 

(Added to collection 

 16th Oct 2008)

Virus ID: 90-44159-70

 

Tube labelled:

90-44159-70; YSD BHK/5 - CPAE/1 - BHK/2;

3-12-08

E1/BHK5/

CPAE1/BHK2

Technical contact in USA:

Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

Reagent also available from: USDA, Aphis, VS, NVSL, PAS, Attn:

Reagents Office P.O. Box 844  Ames, IA 50010 USA

 

nvsluf@aphis.usda.gov

 
NIG1982/11

BTV

Type 22  

Nigeria

(08/08/82)

NK

Shelf C4, 
box C4α
rows G1-H10

 

(Added to collection on

21st Dec 2005)

 

 

(N-421)

Adapted to BHK cells

 

E1/BHK3

[IAH/BHK2]

 

 

 [Seg-2:

Seg-6: AJ586726]

NIG1983/01

 

BTV
Type 22

 

Nigeria
(01/05/83)

 

NK

Shelf C4, 
box C4α
rows E1-F10
 
(Added to 
collection on 
21st Dec 2005)
(N-415)

 

Adapted to 
BHK cells 
 
E1/BHK11
[IAH/BHK2]

 

 

 
NIG1982/12

BTV

Type 22

Nigeria

(13/08/82)

NK

Shelf C4, 
box C4α
rows I1-J10

 

(Added to collection on

21st Dec 2005)

 

N-441

Adapted to BHK cells

 

E1/BHK4

[IAHBHK2]

 

 

 

 

*IAH dsRNA virus collection number: [Country code, year / sample number] rrrr = reference strain vvvv = vaccine strain]

References
1.        Maan S, Maan NS, Samuel AR, O'Hara R, Meyer AJ, Rao S, Mertens PP. (2004) Completion of the sequence analysis and comparisons of genome segment 2 (encoding outer capsid protein VP2) from representative isolates of the 24 bluetongue virus serotypes. Vet Ital. 2004 Oct-Dec;40(4):484-8.      http://www.ncbi.nlm.nih.gov/pubmed/20422574
2. Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC. (2007) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes. Journal of General Virology  88, 621-630.
3. Singh KP, Maan S, Samuel AR, Rao S, Meyer AJ, Mertens PP. (2004) Phylogenetic analysis of bluetongue virus genome segment 6 (encoding VP5) from different serotypes. Vet Ital. 2004 Oct-Dec;40(4):479-83.       http://www.ncbi.nlm.nih.gov/pubmed/20422573
4. St George TD, Standfast HA, Cybinski DH, Dyce AL, Muller MJ, Doherty RL, Carley JG, Filippich C, Frazier CL. (1978) The isolation of a bluetongue virus from Culicoides collected in the Northern Territory of Australia. Aust Vet J. 1978 Mar;54(3):153-4.

 

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