Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae


Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 18 (BTV-18)

in the dsRNA virus collection at  IAH Pirbright


Return to Virus Collection

 Family: ReoviridaeGenus: OrbivirusSpecies: Bluetongue virus - serotype 18 (BTV-18)

Virus Species


(identification method)



(identification method)

Place of original sample, eg country, town [grid reference]

(date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

(Date of virus isolation, where, by whom)

Position of storage in reference freezers


 (Date added to collection)

IAH dsRNA virus collection number:


 [Country code year / sample number]


(Suppliers Isolate Designation)

Adapted to which cell types

Original sample material

(Passage history of sample)

* Original sample supplied to the collection by

person / institution


[IAH, ISIS sample number]





Segments Sequenced /

accession numbers


BTV Type 18

Supplied by OVI

S. Africa*




 Shelf B6,

box B6α

Rows A1-B10


(23 Aug 2005)

(Ref. Strain)





Duplicate samples A1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, Kyung Ki-Do, 440-330, Korea 


Vial B1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009


Vial B2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009


Vial A sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011






Maan et al 2004 (1),

Singh et al 2004 (3),

Maan et al 2007 (2)]


MAR2005/07 BTV-18 Martinique (France)

(Sample taken Dec  2005)
from a
non-vaccinated cow
Shelf C8,
 Box C8α,
 Rows E1-F10
(added to collection 25/08/2010

tube labelled:



Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France

23rd July 2010
Distribution restricted
 by MTA




Initial attempts to identify serotype in 1990 by SNT indicated

type 8


However, type specific RT-PCR  and sequencing (Seg-2)  March 2008 identified type 18. [1] Attempts to detect type 8 with multiple primers sets failed


St. Andrews


Sample taken 4th Sept  1990

Water buffalo blood


Isolated in 1990

Shelf A15,

box A15 β,

rows I1-J10


(Added to collection 

 16th Oct 2008)

Virus ID: 90-44159-47


Tube labelled:

90-44159-59; YSD BHK/4 -



(sample prepared

12th Mar 2008)



Technical contact in USA:  Donna Johnson

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844  Ames IA, 50010 USA.


Reagent also available from: USDA, Aphis, VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA








Maan S, Maan NS, Samuel AR, O'Hara R, Meyer AJ, Rao S, Mertens PP. (2004) Completion of the sequence analysis and comparisons of genome segment 2 (encoding outer capsid protein VP2) from representative isolates of the 24 bluetongue virus serotypes. Vet Ital. 2004 Oct-Dec;40(4):484-8.



Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC. (2007) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes. Journal of General Virology  88, 621-630.


Singh KP, Maan S, Samuel AR, Rao S, Meyer AJ, Mertens PP. (2004) Phylogenetic analysis of bluetongue virus genome segment 6 (encoding VP5) from different serotypes. Vet Ital. 2004 Oct-Dec;40(4):479-83.




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