ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 24 (BTV-24 )

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection » Contents

 

Reference strain BTV-24 2010 2009 2008 2007 2005

 

 

 Family: ReoviridaeGenus: Orbivirus,  Species: Bluetongue virus,  serotype 24 (BTV-24) 

 *IAH dsRNA virus collection number

 

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 

 (identification method)

Place of original sample eg country town

 [grid reference]

 

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 

 Adapted to which cell types 

 

(Passage history of sample)

 

[IAH passage history]

* Original sample supplied to the collection by person / institution

 

[IAH, ISIS sample number]

Comments

 

 References

&

 accession numbers  

RSArrrr/24

BTV-24

 

(western topotype reference strain)

Supplied by OVI
S. Africa*(02/10/87)

NK

Shelf C3, 
box C3α,
rows A1 - B10
 
(added to 
collection on 
15th Nov 2006)

(Ref. Strain)

Adapted to BHK cells

 

(E1/CER4/BHK2)

[IAH/BHK4] 

 

*

Duplicate samples  A1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do,

440-330, Korea

*

Vial B1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial B2 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

* 

Vial A sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

* 

 


[Seg-2:AJ585145,

Seg-6:AJ586730

Maan et al 2004 (1), Singh et al 2004 (4), Maan et al 2007 (5)]

 

 

» contents

2010
ISR2010/23

BTV-24 Identified as BTV-24 by Seg-2 specific

 Real time RT-PCR 

Israel Farhan, close to Jordan border

 

14th July  2010

Isolated from cattle blood at IAH

* Shelf C14,

box  C14 β, Rows C1-D9

 Added
 to collection

5th July 2011

1901/10
KVI Id 78428

[IAH/KC1]

Jacob Brenner

Kimron Institute Israel

 

Samples arrived

 6TH April 2011

 

IAH Stars No. 
A32/11 04
Note vial D10 was not added to freezer  B
ISR2010/19

BTV-24 Identified as BTV-24 by Seg-2 specific

 Real time RT-PCR 

Yitav
Israel

Sample taken November 2010

Isolated from sheep blood

Isolated at IAH

Shelf C14,

box  C14α,

rows C1-D9;

 

 Added to collection June 2011 

3027/10/1

KVI Id 894

 [IAH/KC1]

Jacob Brenner

Kimron Institute Israel

 

Samples arrived

6TH April 2011

 

IAH Stars No.A32/11 08

 Note vials D10 were not added to freezer A & B

ISR2010/11

BTV-24 Identified as BTV-24 by Seg-2 specific

 Real time RT-PCR 

10/10/10

Gilgal, Jordan valley, Israel
Cattle blood

Isolated at IAH

Shelf C11

Box  C11 GAMMA Rows  I and J

 

Added  14/1/11
2695/1 [IAH/KC1]

Jacob Brenner

Kimron Institute Israel

  

Samples arrived 24th November 2010

 

IAH Stars
number

A114/10 08

note: vial was not added to position J10

2009
ISR2009/19
(ISR2010/03)

BTV-24 

Identified as BTV 24 by Seg-2 specific

 Real time RT-PCR 

Israel

 

 Havat Ronit/ Rishpon  

 

 Ovine blood

Isolated from sheep blood  in embryonated chicken eggs at Kimron

Shelf B17

box  β,

rows E1-E10

 

Added to collection on

8th Apr 2010

3283/09/1

ECE1

[IAH/V1]

Jacob Brenner , Kimron Institute Israel

 

Samples arrived 23rd March 2010

 

IAH Stars
number
A24/10 18
Clinical signs: Classical ovine BT symptoms

Note: 10 vials of glycerol stored in freezer A and 10 vials of PDAM stored in freezer B

Outbreak data

ISR2009/18

(ISR2010/04)

BTV-24 

Identified as BTV 24 by Seg-2 specific

 Real time RT-PCR 

Israel

 

Havat Ronit/ Rishpon  

 

From sheep blood

collected

11th Nov 2009 

Isolated
From Sheep blood
in KC cells
at IAH

Shelf B17

box  β,  

rows G1-G10

 

 

Added to collection on

8th Apr 2010

3283/09/2

 [IAH/KC1]

Jacob Brenner , Kimron Institute Israel

 

Samples arrived 23rd March 2010

 

IAH Stars
number

A24/10 13

Clinical signs: Classical ovine BT symptoms

Note: 10 vials of glycerol stored in freezer A and 10 vials of PDAM stored in freezer B

Outbreak data

ISR2009/16

BTV24

 

Identified as BTV 24 by Seg-2 specific

 Real time RT-PCR

Israel,

 from Hadarom, region Eshell,

 

Sheep sample

1st Oct  2009

From Sheep blood

Isolated in
 KC cells
at IAH-P

Shelf B13

box  B13 β,

rows E1-F10

 

Added to collection on  25th Nov 2009

3042/09

[IAH/KC1]

Dr. Hagai Yadin, Kimron Institute Israel  

 

Samples arrived 2nd November 2009

 

[IAH Stars No.

A88/09 01]

outbreak data

ISR2009/12

BTV-24

 

Identified as BTV by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Almog

Sampled

21st May  2009

bovine blood

Isolated in  cells
 at IAH-P

Shelf B10

Box B10 α

Rows Added G1-H10

Added to collection on 13th July 2009

Cow number 631

KC1

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15TH June 2009

 

IAH Stars
number
A55/09 7 (blood)
 

ISR2009/11

BTV-24

 

Identified as BTV by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Kalya

 

sampled

20th May  2009

bovine blood

Isolated in  eggs at Kimron (Israel)

Added to collection on 13th July 2009

Shelf B10

Box B10 α,

Rows Added E1-F10

Cow number 1251

E1

[BHK1 at IAH-P]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15TH June 2009

 

IAH Stars
number
A55/09 6 (eggs)
 

ISR2009/10

BTV-24

 

Identified as BTV by Seg-2 specific

 Real time RT-PCR 

Israel,  from Kalya

 

 sampled

20th May  2009

bovine blood

Isolated in  eggs at Kimron (Israel)

Shelf B10

Box B10 α,

Rows Added C1-D10

Added to collection on 13th July 2009

Cow number 1349

E1

[BHK1 at IAH-P]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15TH June 2009

 

IAH Stars
number
A55/09 5 (eggs)
 

ISR2009/09

BTV-24

 

Identified as BTV by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Kalya

 

Sampled

20th May  2009

cattle blood

Isolated in  cells
at IAH-P

Added to collection on 13th July 2009

Shelf B10

Box B10 α,

Rows A1-B10

Added to collection on 13th July 2009

Cow number 1349

KC1

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15TH June 2009

 

IAH Stars
number
A55/09 5 (blood)
 

ISR2009/08

BTV-24

 

Identified as BTV by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Kalya

 

 Sampled

20th May  2009

cattle blood

Isolated in  eggs at Kimron (Israel)

Shelf B9

Box B9 γ,

Rows Added I1-J10

Added to collection on 13th July 2009

Cow number 1255

E1

[BHK1 at IAH-P]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15TH June 2009

 

IAH Stars
number

A55/09 4 (eggs)

 

ISR2009/07

BTV-24

 

Identified as BTV by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Kalya

 Sampled

20th May  2009

cattle blood

Isolated in KC  cells

at IAH-P

 Shelf B9

Box B9 γ,

Rows Added G1-H10

Added to collection on 13th July 2009

Cow number 1255

KC1

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15TH June 2009

 

IAH Stars
number
A55/09 4 (blood)

ISR2009/06

BTV-24

 

Identified as BTV by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Kalya

 

 Sampled

20th May  2009

cattle blood

Isolated in KC cells

at IAH-P

 Shelf B9

Box B9 γ,

Rows E1-F10

Added to collection on 13th July 2009

Cow number 1215

KC1

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15TH June 2009

 

IAH Stars
number

A55/09 3 (blood)

Vial position  E9  (G) taken out of freezer A   and B on 29th Mar 2012  

ISR2009/05

BTV-24

 

Identified as BTV by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Kalya

 Sampled

20th May  2009

cattle blood

 

Isolated in  eggs at Kimron , Israel

Shelf B9

Box B9 γ,

Rows C1-D10

Added to collection on 13th July 2009

Cow number 1392

E1

[BHK1 at IAH-P]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15TH June 2009

 

IAH Stars
number
A55/09 2 (egg)
 

ISR2009/04

BTV-24

 

Identified as BTV by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Kalya

 

Sampled

20th May  2009

cattle blood

 

Isolated in KC cells at IAH-P

Shelf B9

Box B9 γ,

Rows  A1-B10

Added to collection on 13th July 2009

Cow number 1392

KC1

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

15TH June 2009

 

IAH Stars
number
A55/09 2 (blood)
 
MAR2009/01 BTV-24 Martinique (France)

(Sample taken Jan 2009)
from non-vaccinated cow

Shelf B18,

box B18 γ ,

rows I1-J10

 

(Added to collection

9th  Aug 2010)

tube labelled:

E1B2  2893

E1/BHK2

 

[IAH/BHK1 ]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France

23rd July 2010
Distribution restricted by MTA

[Seg-2: ]
2008
ISR2008/12

BTV-24

 

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

 

Identified as serotype 24 by sequence analysis

Israel,

 from a village called: Hatzav

 

samples

5TH Nov 2008
Isolated on eggs in Israel from ovine spleen.

Shelf B8,

box B8 γ

rows A1-B10

Added to collection on 5th Mar 2009

institute number

592236

E1

[IAH/E1/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars
number
A181/08 7]
 

ISR2008/11

BTV-24

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR [3]

 

Identified as serotype 24 by sequence analysis

Israel,

 from a village called: 

Hatzav

 

samples

taken

20th Nov 2008

From sheep blood

 

Isolated in embryonated chicken eggs in  Israel

Shelf B8

box B8 β,

rows G1-H10

 

Added to collection on 5th Feb 2009

Kimron institute No.

592836

 

E1

[IAH/BHK2]

 

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars
No. A181/08 11]
 
ISR2008/10

BTV-24

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR [3]

 

Identified as serotype 24 by sequence analysis

Israel,

 from a village called: Hura 

 

samples

20th Nov 2008

 

From sheep  spleen

 

Isolated in embryonated chicken eggs in  Israel

Shelf B8,

box B8 β,

rows E1-F10

 

Added to collection on 5th Feb 2009

Kimron institute No. 592898

 

E1

[IAH/BHK2]

 

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars
No. A181/08 10]
 

ISR2008/09

BTV-24

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR [3]

 

Identified as serotype 24 by sequence analysis

Israel,

 from a village called: Beer Sheva 

 

samples

9th Nov 2008

 

From sheep blood

 

Isolated in embryonated chicken eggs in  Israel

Shelf B8,

box B8 β,

rows C1-D10

 

Added to collection on 5th Feb 2009

Kimron institute No.

592395

 

E1

[IAH/BHK2]

 

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars
No. A181/08 9]
 
ISR2008/08

BTV-24

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR [3]

 

Identified as serotype 24 by sequence analysis

Israel,

 from a village called: Beer Sheva

 

samples

9th Nov 2008 
From cattle blood.

Isolated in embryonated chicken eggs in  Israel

Shelf B8,

box B8 β,

rows A1-B10

Added to collection on 30th Jan 2009

Kimron Institute No.

592394

E1

[IAH/BHK2] 

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars
No. A181/08 8]
 
ISR2008/07

BTV-24

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR [3]

 

Identified as serotype 24 by sequence analysis

Israel,

 from a village called: Menuha

 

samples

1st Dec 2008
From cattle blood.

Isolated in embryonated chicken eggs in  Israel

Shelf B8,

box B8 α,

rows I1-J10

 

Added to collection on 30th Jan 2009

Kimron Institute No.

593390

E1

[IAH/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars
No. A181/08 6]
 
ISR2008/06

BTV-24

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR [3]

 

Identified as serotype 24 by sequence analysis

Israel,

 from a village called: Menuha

 

samples

3rd Nov 2008 
From sheep blood

Isolated in embryonated chicken eggs in  Israel

Shelf B8,

box B8 α,

rows G1-H10

Added to collection on 30th Jan 2009

Kimron Institute No. 592148

E1

[IAH/BHK2] 

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars No.
A181/08 5]
 
ISR2008/05

BTV-24

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR [3]

 

Identified as serotype 24 by sequence analysis

Israel,

 from a village called: Elishma

 

samples

24th Nov 2008 
 From sheep blood.

Isolated in embryonated chicken eggs in  Israel

Shelf B8,

box B8 α,

rows E1-F10

 

Added to collection on 30th Jan 2009

Kimron Institute No. 593011

E1

[IAH/BHK2] 

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars No.
A181/08 4] 

*

Duplicate vials position  E1g , taken out of freezers A and B on 6th Sept 2010  

Vial position  F2  (G) taken out of freezer A and B on  29th Mar 2012
*

2007
USA2007/01

BTV- 24

 

Virus serotype identified by RT-PCR using type specific primers  and sequencing (Seg-2) 

[2]

USA: Marion County Florida

 

Sample taken 25th  Sept  2007

White tailed deer  (farmed)

 

Multiple losses and Clinical disease observed in herd

 

Isolated in 2007

Shelf A16,

box A16 α,

rows A1-B10

 

(Added to collection 

 16th Oct 2008)

Virus ID: 07-517030

 

Tube labelled:

517030; IVD BHK1; 10-30-07

 

(sample prepared

30th Oct 2007)

 

 

Isolated from spleen sample

 

 

E1/BHK1

virus isolate supplied by / Reagent also available from:

 : 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

nvsluf@aphis.usda.gov

 

Technical contact in USA:

Donna Johnson

:

Equine and Ovine Viruses Section, Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

Vial position  A1 (P) from freezer A  and B  given to Kyriaki Nomikou 29-Mar-2012 to prepare  RNA to be sent to Sanger  Centre for sequencing

2005

MAR2005/03

BTV-24

Martinique (France)

(Sample taken Jan 2005)

from non-vaccinated cow

Shelf B18,

box B18 γ ,

rows G1-H10

 

(Added to collection

9th  Aug 2010)

tube labelled:

BTV-
24/06.01-127

[IAH/BHK1]

Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France

23rd July 2010

Distribution restricted by MTA

[Seg-2 ]

 

 

References
1.


Maan S, Maan NS, Samuel AR, O'Hara R, Meyer AJ, Rao S, Mertens PP. (2004) Completion of the sequence analysis and comparisons of genome segment 2 (encoding outer capsid protein VP2) from representative isolates of the 24 bluetongue virus serotypes. Vet Ital. 2004 Oct-Dec;40(4):484-8.      http://www.ncbi.nlm.nih.gov/pubmed/20422574

2. 


Mertens PPC, Maan NS, Prasad G, Samuel AR, Shaw AE, Potgieter AC, Anthony SJ, Maan S. (2007) Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains. J Gen Virol. 2007 Oct;88(Pt 10):2811-23.

3.


Shaw AE, Monaghan P, Alpar HO, Anthony S, Darpel KE, Batten CA, Guercio A, Alimena G, Vitale M, Bankowska K, Carpenter S, Jones H, Oura CA, King DP, Elliott H, Mellor PS, Mertens PP. (2007) Development and initial evaluation of a real-time RT-PCR assay to detect bluetongue virus genome segment 1. J Virol Methods. 2007 Nov;145(2):115-26. Epub 2007 Jun 21.  

4.


Singh KP, Maan S, Samuel AR, Rao S, Meyer AJ, Mertens PP. (2004) Phylogenetic analysis of bluetongue virus genome segment 6 (encoding VP5) from different serotypes. Vet Ital. 2004 Oct-Dec;40(4):479-83.       http://www.ncbi.nlm.nih.gov/pubmed/20422573

5.

Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC. (2007) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes. Journal of General Virology  88, 621-630.

 

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