ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

Back to dsRNA virus Homepage 

Home ] virus-nos-by-country ] BTV-Nos.htm ] Virus-nos ] BTV-1 ] BTV-2 ] BTV-3 ] BTV-4 ] [ BTV-5 ] BTV-6 ] BTV-7 ] BTV-8 ] BTV-9 ] BTV-10 ] BTV-11 ] BTV-12 ] BTV-13 ] BTV-14 ] BTV-15 ] BTV-16 ] BTV-17 ] BTV-18 ] BTV-19 ] BTV-20 ] BTV-21 ] BTV-22 ] BTV-23 ] BTV-24 ] BTV-25 ] BTV-26 ] Rescued-BTV ]

Isolates of Bluetongue virus type 5 (BTV-5)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection Contents

 

 

 Family: Reoviridae,  Genus: Orbivirus, Species: Bluetongue virus,  serotype 5 (BTV-5) 

 *IAH dsRNA virus collection number

 

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 

 (identification method)

Place of original sample eg country town

 [grid reference]

 

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

 

(Date of virus isolation, where, by whom)

Position of storage

in reference freezers

 

 (Date added

 to collection)

Suppliers

Isolate Designation

Original sample

material,

 

 Adapted to which

cell types 

 

(Passage history

of sample)

 

[IAH passage

history]

* Original sample

supplied to the

 collection by person / institution

 

[IAH, ISIS

sample number]

 

Comments

 References & accession numbers  

 RSArrrr/05

BTV-5

 

(western topotype

 reference strain) 

Supplied by

OVI

S. Africa* (18/06/82)

NK

Shelf B3,

Box B3γ,

Rows E1-F10  

 

(Added to

collection on

20th May 2005)

(BTV-5 Ref. Strain)

E2/BHK5

 [IAH/BHK1]

*

Duplicate samples E1(g) supplied

on 19th Dec 2007, to JongChul, Jeong

Ja Yeon Agrotech Co., Ltd., 385-6,

ChonChon-Dong,JangAn-Gu, SuWon-City,

KyungKi-Do, 440-330, Korea

*

Vial F1 (p) A&B sent to Mehdi El Harrak,

Biopharma , Morocco  29th June 2009

*

Vial F2 sent to Denis Kolbasov

Russian National Institute of 

Veterinary Virology 29th Sept 2009

* 

Vial E sent to Dr Graham Abdeljelil,

 Institut Pasteur de Tunis 8th March 2011

*

Vial positions E5 (G) taken out of freezer A, and given to Lizzie Morecroft (IAH) on 26th June 2012, corresponding vial in freezer B not removed

*

Vial position E6(g) was   

taken out of Freezer A on 31st

May 2013 and given to

Marcela Gasparini (Pirbright)

to make reference RNA for use in Brazil

 (Corresponding vial in freezer B 

 has  not been removed)

*

 

[Seg-2: KP821101,

Seg-6; KP821582,

Nomikou  et al 2015 (4)]

 

 

[Seg-2:AJ585126

Seg-6:AJ586700, 

Maan et al 2007 (1)

Singh et al 2004 (2)

Maan et al 2004  (3)]

 

 

contents

ISR2011/04 BTV-5
Identified as BTV-5 by

Israel

 

 2011

 
Shelf C17, 
box C17 alpha,

rows A1 - B10

       
ISR2011/01

BTV-5

Identified as BTV-5

by Seg-2 specific

 Real time RT-PCR 

Israel Kalia

 

 

 May 2011

Cattle  Blood

 Isolated through eggs at Kimron

Shelf C16, 
box C16 beta,

rows G1 - H10

 

Added

KVI Id 105638

Lab no: 1405

[IAH/KC1]

Jacob Brenner

Kimron Institute Israel

 

 

Samples arrived

3rd November 2011

 

IAH Stars
number A70/11 01

 
USA2003/05

BTV-5

 

Typed by

Seg-2 RT-PCR

and sequence analyses

Manatee  County FL, USA

 

(sample taken

21st October 2003)

From Cattle

 

no clinical history given.

 

(isolate preparation date 28th July 2006)

Shelf D6, 
box D6γ,

rows I1 - J10

 

(added to collection
on 15th June 2007)

04-280559-7

 

(tube labelled: 280559-7;

IVD BHK/2;

7-28-06)

From blood

 

E1/BHK2

[IAH/BHK4]

virus isolate supplied by :

 

USDA, Aphis, VS, NVSL, PAS

Reagents Office.

PO box 844

Ames .IA 50010

USA

nvsluf@aphis.usda.gov

[S2:]

 

Technical contact in USA: Donna Johnson,

Equine and Ovine Viruses Section,

Diagnostic virus Lab, PO box 844

Ames IA, 50010 USA.

 

original typing attempts

as one of previous US types

were unsuccessful. 

Multiple  other serotypes

 were isolated from this herd

(BTV-2, -3, -10 and

two isolates of unknown serotype: 

see USA2003/04

 

NIG1982/04

BTV Type 5

Nigeria

 (09/08/82)

NK

Shelf C3, 
box C3β,

rows C1 - D10

 

(added to collection

on 18th Nov 2005)

(N-367)

E1/BHK4

[IAHBHK2]

 

 

[S2:]

 

NIG1982/03

BTV

Type 5

 Nigeria (29/08/82)

NK

Shelf C3, 
box C3β,

rows A1 -B10

 

(added to collection

on 18th Nov 2005)

N-409

E1/BHK3

[IAH/BHK2]

*

Vial, A1 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th April 2013 for sequencing. Corresponding vial in freezer B  was  not removed
*

[S6:AJ586702]

 
 CAR1982/03

BTV Type 5

Cameroon (26/10/82)

NK

shelf C2,
box C2β,
rows A1-B10
 
added to 
collection 
 25th Oct 2005  

(82/3)

Spleen

 

 (E1/BHK1)

[IAH/BHK2]

*

Vial, I1 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th April 2013 for sequencing. Corresponding vial in freezer B  was  not removed
*

 
CAR1982/02 BTV Type 5 Cameroon (26/10/82) NK
shelf C2, 
box C2α,
rows I1-J10
 
Added to 
collection on
18th Oct 2005
(82/2) (LN/E1/BHK1)

[IAH/BHK2]

*

Vial, I1 from Freezer A was given to Elizabeth Morecroft (Pirbright) 26th April 2013 for sequencing. Corresponding vial in freezer B  was  not removed
*
S2: S6:AJ586701]

 

*IAH dsRNA virus collection number: [Country code, year / sample number] rrrr = reference strain vvvv = vaccine strain]

References
1.       

 

Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC (2007) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes. J Gen Virol. 88, 621-630

2.

Singh KP, Maan S, Samuel AR, Rao S, Meyer AJ, Mertens PP. (2004) Phylogenetic analysis of bluetongue virus genome segment 6 (encoding VP5) from different serotypes. Vet Ital. 2004 Oct-Dec;4(4):479-83.     http://www.ncbi.nlm.nih.gov/pubmed/20422573http://www.ncbi.nlm.nih.gov/pubmed/20422573 

3.


Maan S, Maan NS, Samuel AR, O'Hara R, Meyer AJ, Rao S, Mertens PP. (2004) Completion of the sequence analysis and comparisons of genome segment 2 (encoding outer capsid protein VP2) from representative isolates of the 24 bluetongue virus serotypes. Vet Ital. 2004 Oct-Dec;40(4):484-8. http://www.ncbi.nlm.nih.gov/pubmed/20422574.

4.

Nomikou K, Hughes J, Wash R, Kellam P, Breard E, Zientara S, Palmarini M, Biek R, Mertens P (2015) Widespread Reassortment Shapes the Evolution and Epidemiology of Bluetongue Virus following European Invasion. PLoS Pathog. 2015 Aug 7;11(8):e1005056.  doi: 10.1371/journal.ppat.1005056. eCollection 2015.

Hit Counter