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Isolates of
Bluetongue virus type 19
(BTV-19)
in the dsRNA virus collection at IAH Pirbright
Return to Virus Collection »
Contents
|
Family: Reoviridae,
Genus: Orbivirus,
Species:
Bluetongue
virus
(BTV) serotype 19
(BTV-19) |
|
Virus
Species
(identification
method) |
Serotype
(identification
method) |
Place
of original sample
eg
country town
[grid reference]
(date
original sample was taken)
|
Isolated from which host species
e.g cow, sheep, etc
(Date
of virus isolation, where, by whom)
|
Position
of storage in reference freezers
(Date
added to collection)
|
IAH
dsRNA virus collection number: [Country code year / sample number]
(Suppliers
Isolate Designation) |
Adapted
to which cell types
Original
sample material
(Passage
history of sample) |
*
Original sample supplied to the collection by
person
/ institution
[IAH,
ISIS
sample number] |
Comments
References
&
accession
numbers |
|
RSArrrr/19 |
BTV-19
(western topotype,
reference strain)
|
Supplied
by OVI
S. Africa*
(13/06/83) |
NK |
Shelf B6,
Box B6α
Rows G1-H10
(23 Aug 2005)
|
(Ref. Strain)
|
E3/BHK6
[IAH/BHK1] |
*
Duplicate samples
G1(g) supplied on
19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech
Co., Ltd., 385-6, ChonChon-Dong, JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea
*
Vial H1 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco 29th
June 2009
*
Vial H2 sent to
Denis Kolbasov, Russian National
Institute of Veterinary Virology 29th
Sept 2009
*
Vial G sent to Dr Graham Abdeljelil,
Institut Pasteur de Tunis 8th
March 2011
*
|
[Seg-2:
AJ585240,
Seg-6:
AJ586722,
Maan et al 2004
(1),
Singh et al 2004
(3),
Maan
et al 2007
(2)]
» contents
|
|
USA2003/04 |
BTV-19
(serotyped
by Seg-2
RT-PCR and
Seg-
sequence comparisons)
|
Manatee County
FL, USA
(sample taken
21st October
2003)
|
From sheep
no clinical
history given.
(isolate preparation
date 12th Nov 2003) |
Shelf E1,
Box E1 α,
rows C1 to D10
(added to
collection on 15th June 2007)
|
04-280559-3
(tube
labelled: 280559-3;
IVD BHK/1;
11-12-03) |
From blood
E1/BHK1
[IAH/BHK4 ] |
virus isolate
supplied by :
USDA, Aphis,
VS, NVSL, PAS Reagents
Office.
PO box 844, Ames .IA 50010, USA
nvsluf@aphis.usda.gov
Technical
contact in USA: Donna Johnson, Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844 Ames IA, 50010
USA.
original
typing attempts as one of previous US types were unsuccessful.
Multiple other serotypes were isolated from this herd (BTV-2, -3,
-10 and two isolates of unknown serotype: see
USA2003/05
|
[Seg-2:]
|
|
DOM1996/04 |
Identified as
BTV-19
by SNT in 1996
Confirmed by
type
specific RT-PCR and subsequent sequence analyses in 2008 |
Dominican Republic
Sample taken
22nd Jan 1996 |
Brahman cattle
blood
Isolated in
1996 |
Shelf A15,
box A15 α,
rows
E1-F10
(Added to collection
16th Oct 2008) |
Virus ID:
96-13303-64
Tube labelled:
96-13303-64; IVD BHK/2;
3-12-08 |
E1/BHK2 |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
Reagent also
available from:
USDA, Aphis,
VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA
nvsluf@aphis.usda.gov |
|
|
DOM1996/02 |
original assays
by SNT
indicated BTV-2
however
type specific
Rt-PCR targeting Seg-2,
using multiple primer
sets
subsequently
indicated type 19 |
Dominican Republic
Sample taken
22nd Jan 1996 |
Brahman cattle
blood
Isolated in
1996 |
Shelf A15,
box A15 α,
rows
C1-D10
(Added to collection
16th Oct 2008) |
Virus ID:
96-13303-72
Tube labelled:
96-13303-72; IVD BHK/2;
3-12-08 |
E1/BHK2 |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
Reagent also
available from:
USDA, Aphis,
VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA
nvsluf@aphis.usda.gov
Vial position C1 (P) from freezer A and B given
to Kyriaki Nomikou 29-Mar-2012 to prepare RNA to be sent to Sanger
Centre for sequencing |
|
|
TAT1990/05 |
Initial attempts
to identify
serotype by SNT were
unsuccessful in 1990
Type specific
RT-PCR and sequencing (Seg-2)
March 2008
identified type 19.
[1]
|
Trinidad:
St. Andrews
Sample taken
4th Sept 1990 |
Water buffalo blood
Isolated in
1990 |
Shelf A15,
box A15 γ,
rows
C1-D10
(Added to collection
16th Oct 2008) |
Virus ID:
90-44159-16
Tube labelled:
90-44159-16; YSD BHK/3 -
3-12-08
(sample prepared
12th Mar 2008) |
E1/BHK4 |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
Reagent also
available from:
USDA, Aphis,
VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA
nvsluf@aphis.usda.gov |
|
| References |
| 1.
|
Maan S, Maan NS, Samuel AR, O'Hara R,
Meyer AJ, Rao S, Mertens PP. (2004) Completion of the sequence
analysis and comparisons of genome segment 2 (encoding outer
capsid protein VP2) from representative isolates of the 24
bluetongue virus serotypes. Vet Ital. 2004 Oct-Dec;40(4):484-8.
http://www.ncbi.nlm.nih.gov/pubmed/20422574
|
| 2. |
Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC. (2007)
Analysis and phylogenetic comparisons of full-length VP2 genes of
the 24 bluetongue virus serotypes. Journal of General Virology
88, 621-630.
|
| 3.
|
Singh KP, Maan S, Samuel AR, Rao S, Meyer AJ, Mertens PP. (2004)
Phylogenetic analysis of bluetongue virus genome segment 6
(encoding VP5) from different serotypes. Vet Ital. 2004
Oct-Dec;40(4):479-83.
http://www.ncbi.nlm.nih.gov/pubmed/20422573
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