ReoID

Phylogenetic sequence analysis and improved diagnostic assay systems for viruses of the family Reoviridae

 

Edited by Peter. P. C. Mertens and Houssam Attoui

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Isolates of Bluetongue virus type 4 (BTV-4)

in the dsRNA virus collection at  IAH Pirbright

 

Return to Virus Collection » Contents

 

 

 

 

 Family: Reoviridae, Genus: Orbivirus, Species: Bluetongue virus serotype 4 (BTV-4)

 *IAH dsRNA virus collection number

( ICTVdb isolate Accession Number)

Virus Species

&

Serotype

 (identification method)

Place of original sample eg country town

 [grid reference]

 (date original sample was taken)

Isolated from which host species e.g cow, sheep, etc

(Date of virus isolation, where, by whom)

Position of storage in reference freezers

 (Date added to collection)

Suppliers

Isolate Designation

Original sample material,

 Adapted to which cell types 

(Passage history of sample)

[IAH passage history]

* Original sample supplied to the collection by person / institution

[IAH, ISIS sample number]

Comments

 References &  accession numbers  

Reference and vaccine strains

RSArrrr/04

BTV - 4

(Reference strain 

 -

'western' topotype)

Supplied by OVI

S. Africa

(06/05/82)

NK

Shelf B3,

box B3β,

rows  C1-D10

Added to collection on

9th May 2005

(Ref. Strain)

 ASOT1

(BHK7)

+

[IAH/BHK2]

Duplicate samples C1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6, ChonChon-Dong,JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea

* 

Duplicate samples, box B3β -  position D2(p)

supplied to Oscar R. González Llamazares

 (Avenida Párroco Pablo Díez 49-57, 24010 - Leon - Spain,  22nd Nov 2007)

* 

Vial D2 (p) A&B sent to Mehdi El Harrak, Biopharma , Morocco  29th June 2009

*

Vial C3 sent to Denis Kolbasov,  Russian National Institute of  Veterinary Virology 29th Sept 2009

*  

One  vials, positions D3 (P)  given to H. Attoui (IAH-P)  20th May 2010: Spare sample relocated to E18 – E18 γ miscellaneous box C position E9 at the bottom of freezer A

*  

Vial E sent to Dr Graham Abdeljelil, Institut Pasteur de Tunis 8th March 2011

*  

Vial position A5(g) was   taken out of Freezer A on 31st May 2013 and given to Marcela Gasparini (Pirbright) to make reference RNA for use in Brazil (Corresponding vial in freezer B  has  not been removed)

*

[Seg-1: KP820946,

Seg-2: KP821066,

Seg-3: KP821188,

Seg-4: KP821308,

Seg-5: KP821428,

Seg-6: KP821548 ,

Seg-7: KP821670,

Seg-8: KP821790,

Seg-9: KP821910,

Seg-10: KP822031,

Nomikou et al 2015]

[S2: AJ585125,

S3:  DQ186794,

S6:AJ586699]

Sellers et al 1979

Maan et al 2007

 » contents

RSAvvv7/04

BTV- 4

S.Africa

 14/Apr/02

NK  

Shelf A1,

Box A1α,  Positions

A1-B10

Vaccine strain

[R8/02 (no7)]

BHK

 [BHK1]

Sue Graham / IAH

 (June 2002)

[S10: ]

RSAvvv5/04

BTV Type 4

S.Africa 14/Apr/02

NK 

shelf A1,

box A1β,

rows G1-H10   

Vaccine strain

[R8/02 (no 5)]

 BHK

[IAH-BHK1]

Sue Graham / IAH

(June 2002)

 » contents

RSAvvv4/04

BTV

Type 4

 [IAH/BHK1]

derived from

RSAvvv1/04

 RSAvvv3/04

BTV Type 4

S.Africa 14/Apr/02

NK

Shelf C4, 
box C4γ
rows A1 - B10
(added to 
collection
  8th Dec 2005)

Vaccine strain

[R8/02 (no 3)

& R7/02]

BHK cells

Previous passage history unknown

BHK1

[IAH/BHK1]

Sue Graham / IAH

(June 2002) 

 PAGE shows two distinct segment 10 bands

[S2: AJ585168,

S6:AJ586676

S9:EU220292]

RSAvvv1/04  

BTV

Type 4

Used to generate

RSAvvv4/04

 RSAvvvv/04

BTV Type 4

 OVI

S. Africa

NK

Shelf B3,

Box B3γ,

Rows I -J10

 (Added to collection on 07/04/03)   

(Vaccine strain)

BHK cells

 [IAH/BHK1]

Sue Graham / IAH

(June 2002)     

Duplicate sample (position J10) given to Carrie Batten, IAH Pirbright  18th Jul 2006

 Vial position J9  (A) given to Carrie Batten (IAH)  20th July 2009.  Vial J9 from Freezer B put in Misc box  B in freezer A position B7

 » contents

[S5:AM778439]

TURvvvv/04

BTV Type 4

(Typed by Serum Neutralisation Test)

Turkey

NK

Shelf A1,

box A1β,  

rows I1-J10  

(added to collection

7th Apr 2003)  

vaccine strain

[IAH-BHK1]

Tubes I1 & J1 from freezer A supplied to  S. Anthony &  H. Jones,  Pirbright Institute 7th June 2004

Vial position J10  (freezer b) given to Carrie Batten (IAH)  20th July 2009.  Vial J10 left in freezer A

 contents

[ S6:AJ586680 ]

2015
ROM2015/04

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR
Romania

bovine blood

(Isolated at PI on 21/10/15)

Shelf D18, 
Box  D18 γ,
 Rows I1-I10

(Added to collection on 10/12/2015)

Animal no: RO504001088200

Sampling date 04/09/15

[PI/KC2] Supplied by : Hristescu Doru Valentin  

NRL for BTV Romania

  A105/15 05
ROM2015/03

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR
Romania

bovine blood

(Isolated in KC cells at TPI on 21/10/15)

Shelf D18, 
Box  D18 γ,
 Rows H1-H10

(Added to collection on 10/12/2015)

Animal no: RO071000087290

Sampling date 31/08/15
[TPI/KC2] Supplied by : Hristescu Doru Valentin

NRL for BTV Romania  

 A105/15 03
ROM2015/02

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR
Romania

From bovine blood

(Isolated in KC cells at TPI on 21/10/15)

Shelf D18, 
Box  D18 γ,
 Rows G1-G10

(Added to collection on 10/12/2015)

Animal no: RO076000086924

Sampling date 31/08/15
[TPI/KC2] Supplied by : Hristescu Doru Valentin

NRL for BTV Romania  

 A105/15 02
ROM2015/01

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR
Romania

From bovine blood

(Isolated in KC cells at PI on 21/10/15)

Shelf D18, 
Box  D18 γ,
 Rows F1-F10

(Added to collection on
10th Dec 2015)

Animal no: RO077000087226

Sampling date 31/08/15
[TPI/KC2] Supplied by : Hristescu Doru Valentin

NRL for BTV Romania  

 A105/15 01
2014
BUL2014/23

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing

Burgas, TrunakBulgaria

 

Animal had clinical signs

Sample taken on
 18th Sept 2014

sheep blood

(isolated at the Pirbright Institute)

Shelf D16  

Box D16α,

row I1 to I10

(Added to

collection on

11th Nov 2014)
101-3 [IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

A133/14  -18
BUL2014/22 BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing

Haskovo/Plevun, Bulgaria

Samples taken 10/07/2014
From sheep blood

(isolated at the Pirbright Institute)
Shelf D14,
Box D14 α,
row J1 to J10

(Added to

collection on  

3rd Oct 2014)
74-11 [IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

A92/14  - 17
BUL2014/21 BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing

Svirachi,

Bulgaria



Sample taken
04/07/2014

From sheep blood

(isolated at the Pirbright Institute)
Shelf D14,
Box D14 α,
row I1 to I10

(Added to

collection on  

3rd Oct 2014)
37-16 [IAH/KC3]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

A76/14  -37
BUL2014/20 BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing

Svirachi, Bulgaria


Sample taken
 
04/07/2014

From sheep blood

(isolated at the Pirbright Institute)
Shelf D14 ,
Box D14 α 
rows H1 to H10

(Added to

collection on  

2nd  Oct 2014)
34-36 [IAH/KC3]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

A76/14  -34
BUL2014/19 BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Burgas/Kosti Bulgaria

Sample taken
 11/07/14

Isolated from sheep blood

(isolated at the Pirbright Institute)

Shelf D13 ,
Box D13γ 
rows H1 to H10

(Added to

collection on

26/09/14)

77-02

[IAH/KC2]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A92/14  -20
Distribution restricted by MTA
BUL2014/18 BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Smolyan/Zlatograd Bulgaria

Sample taken
14/07/14

Isolated from cattle blood

(isolated at the Pirbright Institute)

Shelf D13 ,
Box D13γ 
rows G1 toG10

(Added to

collection on

26/09/14)

83-36

[IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A92/14  -26
Distribution restricted by MTA
BUL2014/17 BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Kurdjali/Gorni Urci, Kandilka Bulgaria

Sample taken
 08/07/14

Isolated from sheep blood

(isolated at the Pirbright Institute)

Shelf D13 ,
Box D13γ
rows F1 to F10

(Added to

collection on

26/09/14)

68-01

[IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A92/14  -11
Distribution restricted by MTA
BUL2014/16 BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Haskovo/Gugutka Bulgaria


Sample taken
07/07/14

Isolated from sheep blood

(isolated at the Pirbright Institute)

Shelf D13 ,
Box D13γ
rows E1 to E10

(Added to

collection on

26/09/14)

60-72

[IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

A92/14  - 03
E1 removed from freezers A&B 21/04/2015:
Ref: Transfer to Switzerland:
PT-15-002-CB

Distribution restricted by MTA
BUL2014/14

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Mandritsa, Bulgaria

Sample taken 04/07/14

Goat blood

(isolated at the Pirbright Institute

K. Nomikou on 23/7/2014)

Shelf  13

Box 13a 

Rows H1-H10

 

(Added to

collection on

15th Aug 2014)
50-126

[TPI/KC1BHK1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A76/14  -50
Distribution restricted by MTA

Derived from BUL2014/06
BUL2014/13

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Bulgaria  

Svirachi

Blood sample taken from sheep 

04.07.2014

sheep blood

(isolated at the Pirbright Institute
K. Nomikou on 23/7/2014)

Shelf  13

Box 13 alpha 

Rows G1-G10

 

(Added to

collection on

15th Aug 2014)
37-16

[TPI/KC1BHK1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

A76/14  -37
Distribution restricted by MTA
BUL2014/12

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Bulgaria  

Svirachi

Blood sample taken from goat 

04.07.2014

Isolated from goat blood

(isolated at the Pirbright Institute  by K. Nomikou on 23/7/2014)

Shelf    D12

Box     D12γ

Rows J1-J10

 

 (Added to

collection on

30th July 2014)
38-11

[IAH/KC1BHK1]

Supplied by : Simona Tchakarova,

 NRL for BTV Bulgaria

A76/14  -38
Distribution restricted by MTA

derived from
BUL2014/05
BUL2014/11

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Bulgaria  

Svirachi

Blood sample taken from sheep

 

04.07.2014

Isolatyed from sheep blood

(isolated at the Pirbright Institute

 by K. Nomikou on 23/7/2014)

Shelf    D12

Box     D12γ

Rows I1-I10

  

(Added to

collection on

30th July 2014)
38-11

[IAH/KC1BHK1]

Supplied by : Simona Tchakarova,

 NRL for BTV Bulgaria

A76/14  -38
Distribution restricted by MTA

derived from
BUL2014/04
BUL2014/10

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Bulgaria,  

Svirachi

Blood sample taken from sheep 

04.07.2014

Isolated from sheep blood

(isolated at the Pirbright Institute  by K. Nomikou on 23/7/2014)

Shelf    D12

Box     D12γ

Rows H1-H10

 

(Added to

collection on

30th July 2014)
35-43 [IAH/KC1BHK1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

A76/14  -35
Distribution restricted by MTA

derived from
BUL2014/03
BUL2014/09

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Bulgaria  

Svirachi

Blood sample taken from sheep

  

04.07.2014

Isolated from sheep blood

(isolated at the Pirbright Institute by K. Nomikou on 23/7/2014)

Shelf    D12

Box     D12γ

Rows G1-G10

  

(Added to

collection on

30th July 2014)
34-36

[IAH/KC1BHK1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A76/14  -34
Distribution restricted by MTA
BUL2014/08

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Bulgaria , Mandritsa

 

Blood sample taken from goat

 04.07.2014

Isolated from goat blood

(isolated at the Pirbright Institute by K. Nomikou on 23/7/2014)

Shelf    D12

Box     D12γ

Rows F1-F10

 

 (Added to

collection on

30th July 2014)
25-90

[IAH/KC1BHK1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A76/14  -25
Distribution restricted by MTA

derived from
BUL2014/02
BUL2014/07

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2 and sequencing

Bulgaria,   Mandritsa

 

Blood sample taken from goat

04.07.2014

goat blood

(isolated at the Pirbright Institute by K. Nomikou on 23/7/2014)

Shelf    D12

Box     D12γ

Rows E1-E10

 

 

(Added to

collection on

30th July 2014)
22-87

[IAH/KC1BHK1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

A76/14  -22
Distribution restricted by MTA

derived from
BUL2014/01
BUL2014/06

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2

Mandritsa, Bulgaria

Sample taken 04/07/14

Goat blood

(isolated at the Pirbright Institute

17th Jul 2014)

Shelf   13

Box   13β 

Rows D1-D10

 

(Added to

collection on

18th Aug 2014)

50-126

[IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A76/14  -50
Distribution restricted by MTA

Used to generate
BUL2014/14
BUL2014/05

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2

Bulgaria  

Svirachi

Blood sample taken from goat 

04.07.2014

Goat blood

(isolated at the Pirbright Institute

17th Jul 2014)

Shelf    13

Box   13β 

Rows C1-C10

 

(Added to

collection on

18th Aug 2014)

40-20

[IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A76/14  -40
Distribution restricted by MTA

Used to generate
BUL2014/12
BUL2014/04

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2

Bulgaria  

Svirachi

Blood sample taken from sheep 

04.07.2014

sheep blood

(isolated at the Pirbright Institute

on 17th Jul 2014)

Shelf    13

Box     13β

Rows B1-B10

 

(Added to

collection on

18th Aug 2014)

38-11

[IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A76/14  -38
Distribution restricted by MTA

Used to generate
BUL2014/11
BUL2014/03

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2

Bulgaria  

Svirachi

Blood sample taken from sheep 

04.07.2014

Sheep blood

(isolated at the Pirbright Institute

on 17th Jul 2014)

Shelf    13

Box   13β

Rows A1-A10

 

(Added to

collection on

15th Aug 2014)

35-43

[IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A76/14  -35
Distribution restricted by MTA

Used to generate
BUL2014/10
BUL2014/02

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2

Bulgaria , Mandritsa

 

Blood sample taken from goat 04.07.2014

Goat blood

(isolated at the Pirbright Institute

 on 17th Jul 2014)

Shelf    13

Box   13a 

Rows J1-J9

 

(Added to

collection on

15th Aug 2014)

25-90

[IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A76/14  -25
Distribution restricted by MTA

Used to generate BUL2014/08

9 vials only
BUL2014/01

BTV-4

Identified as BTV-4 by type-specific RT-PCR targeting Seg-2

Bulgaria,  

 Mandritsa

 

Blood sample taken from goat 04.07.2014

Goat blood

(isolated at the Pirbright Institute on 17th Jul 2014)

Shelf    13

Box   13a 

Rows I1-I10

 

(Added to

collection on

15th Aug 2014)

22-87

[IAH/KC1]

Supplied by : Simona Tchakarova,

NRL for BTV Bulgaria

 

A76/14  -22
Distribution restricted by MTA

Used to generate
BUL2014/07
GRE2014/08

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Greece,
Chalkidiki,
sample taken
13th Aug 2014

sheep blood

(isolated at the
Pirbright Institute)

Shelf D16

Box D16 α 

row H1 to H10, 

(Added to

collection

11th  Nov 2014)
none [IAH/KC2]

Supplied by : Konstantia Tasioudi

NRL for BTV Greece

A110/14 09
GRE2014/07 BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Greece,
 Chalkidiki
 

2014

From sheep blood

(isolated at the Pirbright Institute)
Shelf D14,
Box D14 α 
rows G1 toG10 
none [TPI/KC1]

Supplied by : Konstantia Tasioudi

NRL for BTV Greece

A110/14 - 10
GRE2014/06 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing
Greece
Lakonia

2014

From sheep blood

(isolated at the Pirbright Institute)
Shelf D14,
 Box D14 α 
rows F1 to F10

(Added to

collection 2nd

Oct 2014)
None [TPI/KC1]

Supplied by : Konstantia Tasioudi

NRL for BTV Greece

A110/14 - 07
GRE2014/05 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing
Greece,
 Lakonia
 

2014

sheep blood

(isolated at the Pirbright Institute)
Shelf D14,
 Box D14 α 
rows E1 to E10

(Added to

collection 2nd

Oct 2014)
None

[TPI/KC1]

Supplied by : Konstantia Tasioudi

NRL for BTV Greece

A110/14 - 05
GRE2014/04 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing
Greece,
Karditsa

2014

sheep blood

(isolated at the Pirbright Institute)
Shelf D14,
Box D14 α,
tubes D1 to D10
None

[IAH/KC1]

Supplied by : Konstantia Tasioudi

NRL for BTV Greece

A110/14 - 04
GRE2014/03 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing
Greece,
 Lesvos
 

2014

sheep blood

(isolated at the

 Pirbright Institute)

Shelf D14,
Box D14 α,
tubes C1 to C10 

(Added to

collection 2nd

Oct 2014)
None

[TPI/KC1]

Supplied by : Konstantia Tasioudi

NRL for BTV Greece

A110/14 - 03
GRE2014/02 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing
Greece,

Thessaloniki region


2014

sheep blood

(isolated at the Pirbright Institute)

Shelf D14,
Box D14 α,
rows B1 to B10 

(Added to

collection 2nd

Oct 2014)
None

[TPI/KC1]

Supplied by : Konstantia Tasioudi

NRL for BTV Greece

A110/14 - 02
GRE2014/01 BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Greece

Peloponnesus region


2014

sheep blood

(isolated at the Pirbright Institute)

Shelf D14,
Box D14 α,
rows A1 to A10 
 
none

[TPI/KC3]

Supplied by : Konstantia Tasioudi

NRL for BTV Greece

A73/14 - 01
HUN2014/01

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Hódmezővásárhely (Csográd Counthy), Southern Hungary.

From cattle blood

Sampling date 10/10/14

Shelf D18

Box  D18 β

Rows C1-10

 

(Added to

collection on 12/06/2015

A158/14 01

[TPI/KC1]

Supplied by :

Dr. Hornyák Ákos NRL for BTV

Hungary

A158/14 01
KOS2014/09

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Viti, Kosovo

Sample taken 2014

sheep blood

(isolated at the Pirbright Institute)

Shelf D16

Box D16γ

row B1 to B10

(Added to

collection

11th  Nov 2014)
03293416 [TPI/KC2]

Supplied by : Shaban Gollopeni

NRL for BTV Kosovo

A120/14 - 146
KOS2014/08

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Shterpc, Kosovo

Sample taken
 2014

SHEEP blood

(isolated at the Pirbright Institute)

Shelf D16  

Box D16γ

rows A1 to A10

(Added to

collection

11th  Nov 2014)
03 [IAH/KC2]

Supplied by : Shaban Gollopeni

NRL for BTV Kosovo

A120/14 - 139
KOS2014/07

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
GJILAN, Kosovo

Sample taken 2014

sheep blood

(isolated at the Pirbright Institute)

Shelf D16  

Box D16 β 

rows J1 to J10

(Added to

collection

11th  Nov 2014)
04590705 [IAH/KC2]

Supplied by : Shaban Gollopeni

NRL for BTV Kosovo

A120/14 - 135
KOS2014/06

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Viti, Kosovo

Sample taken 2014

sheep blood

(isolated at the Pirbright Institute)

Shelf D16  

Box D16β,

row I1 to I10

(Added to

collection

11th  Nov 2014)
03290217 [IAH/KC2]

Supplied by : Shaban Gollopeni

NRL for BTV Kosovo

A120/14 - 131
KOS2014/05

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Prishtine, Kosovo

Sample taken  2014

sheep blood

(isolated at the Pirbright Institute)

Shelf D16  

Box D16 β 

Row  H1 to H10

(Added to

collection

11th  Nov 2014)
04771029 [IAH/KC2]

Supplied by : Shaban Gollopeni

NRL for BTV Kosovo

A120/14 - 119
KOS2014/04

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Shterpc, Kosovo

Sample taken 2014

sheep blood

(isolated at the Pirbright Institute)

Shelf D16  

Box D16 β 

row  G1 toG10

(Added to

collection

11th  Nov 2014)
16 [IAH/KC2]

Supplied by : Shaban Gollopeni

NRL for BTV Kosovo

A120/14 - 101
KOS2014/03 BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Viti, Kosovo

2014
Isolated from

sheep blood


(isolated at the Pirbright Institute)
Shelf D14,
Box D14 β,
tubes C1 to C10

(Added to

collection

3rd Oct 2014)
02641916 [IAH/KC1]

Supplied by : Shaban Gollopeni

NRL for BTV Kosovo

A120/14 - 129
KOS2014/02 BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Viti, Kosovo

2014
Isolated from

sheep blood


(isolated at the Pirbright Institute)
Shelf D14,
Box D14 β,
rows B1 to B10

(Added to

collection

3rd Oct 2014)
04722434 [IAH/KC1]

Supplied by : Shaban Gollopeni

NRL for BTV Kosovo

A120/14 - 128
KOS2014/01 BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Prishtine, Kosovo

2014
Isolated from

sheep blood


(isolated at the Pirbright Institute)
Shelf D14,
Box D14 β,
rows A1 to A10

(Added to

collection

3rd Oct 2014)
00466110 [IAH/KC1]

Supplied by : Shaban Gollopeni

NRL for BTV Kosovo

A120/14 - 118
ROM2014/12 BTV-4 Romania from cattle

Shelf D18

Box  D18 γ

Rows A1-10

 

(Added to

collection on 12/06/2015)

RO108215

6775

A222/14 21

 [TPI/V1]

Supplied by: Hristescu Doru Valentin

ROM2014/11

BTV-4

Romania

from cattle

Shelf D18

Box  D18 β

Rows J1-10

 

(Added to

collection on 12/06/2015)

RO1060000

04635

A222/14 20

  

[TPI/V1]

Supplied by: Hristescu Doru Valentin

ROM2014/10

BTV-4

Romania

from cattle

Shelf D18

Box  D18 β

Rows I1-10

 

(Added to

collection on 12/06/2015)

RO1040000

34677

A222/14 19

 

[TPI/V1]

Supplied by: Hristescu Doru Valentin

ROM2014/09 BTV-4 Romania from cattle

Shelf D18

Box  D18 γ

Rows D1-10

 

(Added to

collection on 12/06/2015)

RO2040000

47231

A222/14 24

[TPI/V1]

Supplied by: Hristescu Doru Valentin
ROM2014/08 BTV-4 Romania from cattle

Shelf D18

Box  D18 γ

Rows C1-10

 

(Added to

collection on 12/06/2015)

RO10600

0002794

A222/14 23

[TPI/V1]

Supplied by: Hristescu Doru Valentin
ROM2014/07 BTV-4 Romania from cattle

Shelf D18

Box  D18 γ

Rows B1-10

 

(Added to

collection on 12/06/2015)

RO3120000

80350

A222/14 22

[TPI/V1]

Supplied by: Hristescu Doru Valentin
SER2014/05 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing
Medvedja, Serbia

Sample taken
30/08/14
 

Isolated from

sheep blood

(isolated at the Pirbright Institute)

Shelf D14,
 Box D14 β 
 rows H1 to H10

(Added to

collection on

 6th Oct 2014)
B8514375 [IAH/KC1]

Supplied by : Vesna Milicevic

NRL for BTV Serbia

A114/14 - 10
SER2014/04 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing
Medvedja, Serbia

Sample taken
30/08/14
 

Isolated from

sheep blood

(isolated at the Pirbright Institute)

Shelf D14,
 Box D14 β 
rows G1 toG10

(Added to

collection on

 6th Oct 2014)
D8639741 [IAH/KC1]

Supplied by : Vesna Milicevic

NRL for BTV Serbia

A114/14 - 07
SER2014/03 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing

Medvedja, Serbia

Samples taken
30/08/14

Isolated from

sheep blood

(isolated at the Pirbright Institute)

Shelf D14,
Box D14 β
rows F1 to F10

(Added to

collection on

 6th Oct 2014)
F0867528 [IAH/KC1]

Supplied by : Vesna Milicevic

NRL for BTV Serbia

A114/14 - 06
SER2014/02 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing

Sijarinska Banja,

Serbia

Samples taken
 30/08/14

Isolated from

cattle blood

(isolated at the Pirbright Institute)

Shelf D14,
Box D14 β
rows E1 to E10

(Added to

collection on

 6th Oct 2014)
7171697695 [IAH/KC1]

Supplied by : Vesna Milicevic

NRL for BTV Serbia

A114/14 - 02
SER2014/01 BTV-4

Identified as BTV-4
  by type-specific RT-PCR targeting Seg-2 and sequencing

Sijarinska Banja,

Serbia

Samples taken
 30/08/14

Isolated from

cattle blood

(isolated at the Pirbright Institute)

Shelf D14,
Box D14 β,
tubes D1 to D10

(Added to

collection on

 6th Oct 2014)
7100359736 [IAH/KC1]

Supplied by : Vesna Milicevic

NRL for BTV Serbia

A114/14 - 01
SPA2014/07

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR

Spain

Cataluña
Isolated in  Laboratoire Central Verterinaire (LCV) from the blood of  an imported  Romanian calf 2014;

Animal  Reg: 3497/14 sample14
Shelf E7,
Box E7α,
Tubes C1 to C10

(Added to collection on 10/12/2015)

BTV4 SPA 2014/03 3P BHK 27/03/2015

(Cod. 194)

Cell culture

KC1 17/02/2015  BHK2 27/03/2015

  [PI/BHK 1](passaged at PI on 03/11/15)
Supplied by : Ruben Villalba Martinez  

NRL for BTV Spain  

A127/15 07
MTA
SPA2014/06

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR

Spain

Andalucía

Isolated in  Laboratoire Central Verterinaire (LCV) sheep blood

Animal Reg: 25921/14 sample 3

Shelf D18,
Box D18γ,
Tubes J1 to J10

(Added to collection on 10/12/2015)
BTV4 SPA 2014/02
 4P BHK 29/01/15
 

 
(Cod. 193)

Cell culture 

KC1 28/10/2014 BHK329/01/2015 

 [PI/BHK1] (passaged at PI on 03/11/15) 

Supplied by : Ruben Villalba Martinez  

NRL for BTV Spain  

A127/15 06
MTA
SPA2014/05 BTV-4 Extremadura, Spain

From cattle blood

(isolated at TPI)

Shelf D18

Box  D18 β

Rows H1-10

 

(Added to

collection on 12/06/2015)
Reg 2731/14 Sample 1

A219/14 07

 

[IAH/KC3]
Supplied by: Ruben Villalba Martinez LCV
SPA2014/04 BTV-4 Extremadura, Spain

From cattle blood

(isolated at TPI)

Shelf D18

Box  D18 β

Rows G1-10

 

(Added to

collection on 12/06/2015)
Reg 2728/14 Sample 4

A219/14 05

 

[IAH/KC3]
Supplied by: Ruben Villalba Martinez LCV
SPA2014/03

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR
Andalucia Spain From sheep blood

Shelf D18

Box  D18 β

Rows F1-10

 

(Added to

collection on 12/06/2015)
2634/14 sample 20

A219/14 04

[IAH/KC1]

Supplied by :

Montserrat Agüero

Laboratorio Central Veterinario-Sanidad Animal Ctra M-106, km 1,4

28110 Algete (Madrid)

20/11/14

A219/14 04
SPA2014/02

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR
Castilla – La Mancha Spain

From sheep blood

Shelf D18

Box  D18 β

Rows E1-10

 

(Added to

collection on 12/06/2015)
2633/14 sample 3

A219/14 02

[IAH/KC3]

Supplied by :

Montserrat Agüero

Laboratorio Central Veterinario-Sanidad Animal Ctra M-106, km 1,4

28110 Algete (Madrid)

20/11/14

A219/14 01
SPA2014/01

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR
Andalucia Spain

From sheep blood

(isolated in LCV, Spain)

Shelf D18

Box  D18 β

Rows D1-10

 

(Added to

collection on 12/06/2015)
2592/14 sample 3

A219/14 01

[IAH/KC1]

Supplied by :

Montserrat Agüero

Laboratorio Central Veterinario-Sanidad Animal Ctra M-106, km 1,4

28110 Algete (Madrid)

20/11/14
2013
SPA2013/01

BTV-4

Identified as BTV-4 by serotype-specific RT-PCR
Andalucia Spain

From sheep blood

(isolated at LCV, Spain)

Shelf D18

Box  E18 γ

Rows A1-10

 

(Added to

collection on 12/06/2015)
3303/13 sample 11

E1/3BHK Batch 3p BHK

[TPI/BHK1]

Supplied by :

Montserrat Agüero

Laboratorio Central Veterinario-Sanidad Animal Ctra M-106, km 1,4

28110 Algete (Madrid
2012
GRE2012/06

BTV-4

 

 Identified as BTV-4  by Seg-2 specific

 Real time RT-PCR 

and Seg-2 sequence analysis

Rhodes island, November 2012 Sheep blood Shelf D9,
box D9 β,
rows E1 to F10

(Added to collection on
18th Dec 2013)
  [TPI/KC1BHK1]

Konstantia Tasioudi

Ministry of Rural development and food, Institute of infectious and parasitic diseases, Athens, Greece

 
 
A200/12-04
Received 12/12/2012

Derived from GRE2012/03

 

Distribution restricted by MTA*

GRE2012/05

BTV-4

 

 Identified as BTV-4  by Seg-2 specific

 Real time RT-PCR 

and Seg-2 sequence analysis

Rhodes island, November 2012 Sheep blood Shelf D9,
box D9 β,
rows C1 to D10

(Added to collection on
18th Dec 2013)
  [TPI/KC1BHK1]

Konstantia Tasioudi

Ministry of Rural development and food, Institute of infectious and parasitic diseases, Athens, Greece

 
 
A200/12 - 03
Received 12/12/2012

Derived from GRE2012/02

 

Distribution restricted by MTA*

GRE2012/04

BTV-4

 

 Identified as BTV-4  by Seg-2 specific

 Real time RT-PCR 

and Seg-2 sequence analysis

Rhodes island, November 2012 Sheep blood Shelf D9,
box D9 β,
rows A1 to B10

(Added to collection on
18th Dec 2013)
  [TPI/KC1BHK1]

Konstantia Tasioudi

Ministry of Rural development and food, Institute of infectious and parasitic diseases, Athens, Greece

  
A200/12 - 02
Received 12/12/2012

Derived from GRE2012/01

 

 

Distribution restricted by MTA*

GRE2012/03

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Rhodes island, November 2012 From sheep blood

Isolated in KC cells
at TPI
13th Dec 2012

Shelf D7   

Box D7 gamma 

row F1 to G10

added to collection

2nd Jan 2013

[TPI/KC1]

A200/12 04

Konstantia Tasioudi

Ministry of Rural development and food, Institute of infectious and parasitic diseases, Athens, Greece

Received 12/12/2012

*
No vial was added to position G10 in freezer B
*
Vials H10 were removed from freezer A & B
*
GRE2012/02

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Rhodes island, November 2012 From sheep blood

Isolated in KC cells
at TPI
13th Dec 2012

Shelf D7   

Box D7 gamma 

row  E1 to F10

added to collection

2nd Jan 2013

  [TPI/KC1]

A200/12 03

Konstantia Tasioudi

Ministry of Rural development and food, Institute of infectious and parasitic diseases, Athens, Greece

Received 12/12/2012


*
Vials F10 were removed from freezer A & B
*
 vial E10 was removed from freezer A
*
GRE2012/01

BTV-4

Identified as BTV-4  by type-specific RT-PCR targeting Seg-2 and sequencing
Rhodes island, November 2012

From sheep blood

Isolated in KC cells
at TPI
13th Dec 2012

Shelf D7

Box D7 gamma

Rows C1-C10 (G);

D1- D10 (P)

 in freezerr A&B:

 

[TPI/KC1]
 

A200/12 02

Konstantia Tasioudi

Ministry of Rural development and food, Institute of infectious and parasitic diseases, Athens, Greece

Received 12/12/2012

*
No vial was added to position D10 in freezer B
*
Vials C10, D10 were removed from freezer A & C10 from freezer B
*
vial D9 was removed from freezer B.
*
2011
CYP2011/10

BTV-4

 

Note identification

of serotype

 needs confirmation

Cyprus
2011

Shelf D10,

box D10 alpha,

rows C1-D10

       
CYP2011/09

BTV-4 Identified as BTV-4 by Seg-2 specific

Real time RT-PCR  and sequence analyses

Cyprus

Poli Chryscchous, region Paphos

 

Sample taken

21st Oct 2011

From sheep blood

 

Isolated in KC cells atTPI Pirbright

Shelf D10,

box D10 alpha,

rows G1-H10

 

(Added to collection on

18th Mar 2014)

10432/25 Animal id: CY246485554 [TPI/KC1BHK1]

Vasiliki Christodoulou

Virology lab, nicosia

 

Samples arrived 7th November 2011

  TPI Stars number A72/11 27

Outbreak Data

 

*

Derived from CYP2011/04

CYP2011/08

BTV-4 Identified as BTV-4 by Seg-2 specific

Real time RT-PCR  and sequence analyses

 

 

Cyprus

Poli Chryscchous, region Paphos

 

Sample taken

20th Oct 2011

From sheep blood

 

Isolated at TPI Pirbright in KC cells

Shelf D10,

box D10 alpha,

rows E1-F10

 

(Added to

collection on

18th of Mar 2014)

10418/5

Animal id: CY236595098

[TPI/KC1BHK1]

Vasiliki Christodoulou

Virology lab, nicosia

 

Samples arrived 7th November 2011

TPI stars number

A72/11 13

Outbreak Data

 

*

Derived from

CYP2011/03

CYP2011/07

BTV-4

 Identified as BTV-4 by Seg-2 specific

Real time RT-PCR  and Seg-2 sequence analyses

Note this isolate was  initially identified as

BTV-16

Cyprus

Poli Chryscchous, region Paphos

 

Sample taken

20th Oct 2011

From sheep blood


Isolated in KC cells at IAH-P

Shelf C16

Box  C16 gamma

 Rows A1 to B10

 

(Added to

collection on

18th Mar 2014)

10418/1

Animal id:CY22181637

[TPI/KC1BHK1]

Vasiliki Christodoulou

Virology lab, nicosia

 

Samples arrived 7th November 2011

TPI stars number A72/11 09

Outbreak Data

 

*

Derived from CYP2011/02

CYP2011/06

BTV-4 Identified

 as BTV-4by

 Seg-2 specific

 Real time RT-PCR and Seg-2 sequence analyses

 

Cyprus

Poli Chryscchous, region Paphos

 

20th Oct 2011

Isolated from sheep blood


Isolated in KC cells at IAH-P

Shelf C17,

box C17 alpha

rows C1-D10 

Added  to collection on

 

Shelf D10,

box D10 α,  

Rows A1 to B10

(Added to

collection on

18th Mar 2014)

10417/10

Animal Number CY222252922
[TPI/KC1BHK1]

Vasiliki Christodoulou

Virology lab, nicosia

 

Samples arrived 7th November 2011

 

IAH Stars
number
A72/11 07

Outbreak Data

 

*

Derived from CYP2011/01

CYP2011/05

BTV-4 Identified as BTV-4by Seg-2 specific

 Real time RT-PCR

Cyprus

 

 Poli Chryscchous,  region Paphos

Sample taken

21st Oct 2011

From sheep blood

 
Isolated in KC cells at IAH-P

Shelf C16,

box C16 beta,

rows E1-F10

 

Added  to collection on

10432/30

Animal id: CY236607625

[KC1]

Vasiliki Christodoulou

Virology lab, nicosia

 

Samples arrived 7th November 2011

 

IAH Stars
number

A72/11 31

Vials E10 &F10 were removed from
freezer A & B.

CYP2011/04

BTV-4 Identified as BTV-4by Seg-2 specific

 Real time RT-PCR

Cyprus

 Poli Chryscchous,  region Paphos

Sample taken

21st Oct 2011

From sheep blood

 
Isolated in KC cells at IAH-P

Shelf C16,

box C16 beta,

rows C1-D10

 

Added  to collection on

10432/25 Animal id: CY246485554 [KC1]

Vasiliki Christodoulou

Virology lab, nicosia

 

Samples arrived 7th November 2011

 

IAH Stars
number

A72/11 27

Vials C10 were removed from freezer A & B.

CYP2011/03

BTV-4 Identified as BTV-4by Seg-2 specific

 Real time RT-PCR

Cyprus

 Poli Chryscchous,  region Paphos

Sample taken

20th Oct 2011

From sheep blood

 
Isolated in KC cells at IAH-P

Shelf C16,

box C16 beta,

rows A1-B10

 

Added  to collection on

10418/5

Animal id: CY236595098

[KC1]

Vasiliki Christodoulou

Virology lab, nicosia

 

Samples arrived 7th November 2011

 

IAH Stars
number

A72/11 13

Vials A10 were removed from freezer A & B.

CYP2011/02

BTV-4 Identified as BTV-4by Seg-2 specific

 Real time RT-PCR

Cyprus

 Poli Chryscchous,  region Paphos

Sample taken

20th Oct 2011

From sheep blood

 
Isolated in KC cells at IAH-P

Shelf C16,

box C16 α,

rows I1-J10

 

Added  to collection on

10418/1

Animal id:CY22181637

[KC1]

Vasiliki Christodoulou

Virology lab, nicosia

 

Samples arrived 7th November 2011

 

IAH Stars
number

A72/11 09

CYP2011/01

BTV-4 Identified as BTV-4by Seg-2 specific

 Real time RT-PCR

Cyprus

Poli Chryscchous,  region Paphos

20th Oct 2011

From sheep blood

 
Isolated in KC cells at IAH-P

Shelf C16,

box C16 α,

rows G1-H10

  

 

Added  to collection on

10417/10

Animal Number CY222252922

[KC1]

Vasiliki Christodoulou

Virology lab, nicosia

 

Samples arrived 7th  November 2011

 

IAH Stars
number
A72/11 07

Vials H10 were removed from freezer A & B.

2010
ARG2010/01

BTV-4 Identified as BTV-4 by Seg-2 specific

 Real time RT-PCR and confirmed by sequence analysis

From Argentina,

Corrientes province.

  (blood sample collected

28th  Oct 2009)

Isolated from cattle blood

 

Isolated at


Instituto de Virología
INTA Castelar
Buenos Aires. Argentina by Dra. María José Dus Santos

Shelf D8,

box  D8 α,

rows I1-J10

 

(Added to

collection on

5th Nov 2013)
4/ARG/01/2010

CRL1660 passages2 /BHK3

[BHK1]

Dra. María José Dus Santos
Instituto de Virología
INTA Castelar
Buenos Aires. Argentina.

Sent
19th Sep 2013
Distribution restricted by MTA
ISR2010/25

* BTV-4

 

 Identified as BTV-4 by

Seg-2 specific

 Real time RT-PCR

Israel

Isolated from cattle blood at IAH

Shelf C14,

box  C14 α,

rows G1-H10

 

 Added to collection 

4th July 2011

Calf purchased for KVI

 

872 KVI Id

[IAH/KC1]

Jacob Brenner, Kimron Institute Israel  

Samples arrived 6TH April 2011

 

IAH Stars
number

A32/11 7

* Note original blood positive for BTV-4 and 8

ISR2010/14

BTV-4 Identified as BTV-4 by Seg-2 specific

 Real time RT-PCR 

14/10/10

Lachis, Israel
Sheep blood

* Shelf C12,

box  C12 β,

rows E and F

Added  to collection

21st Jan 2011

   
2769/2 [IAH/KC1]

Jacob Brenner,Kimron Institute Israel

  

Samples arrived 24th November 2010

 

IAH Stars No.
A114/10 12
Note : Vials F10 an E 10 were not added to freezer A &  B
ISR2010/09

BTV-4

 Identified as BTV-4 by Seg-2 specific

 Real time RT-PCR 

Israel

Kfar Glicson,

Sample taken

5th Oct 2010

Cattle blood

Shelf C11

Box  C11 γ,

rows  E and F

 

Added  to collection on

14th Jan 2011

2679

[IAH/KC1]

Jacob Brenner

Kimron Institute Israel

 

 

Samples arrived 24th November 2010

 

IAH Stars
number

A114/10 06

Vial F10 was not added to freezer B

ISR2010/05

BTV-4 

Identified as BTV 4 by Seg-2 specific

 Real time RT-PCR 

Israel

 from Bet Kama

Sample taken

7th Jan 2010

Cattle blood

Isolated in
 KC cells at IAH-P

Shelf B17,

box  β,

rows I1-I10

 

Added to collection
8th Apr 2009
1047/10/2 [IAH/KC1]

From:

Jacob Brenner , Kimron Institute Israel

 

Samples arrived 23rd March 2010

IAH Stars
number
A24/10 15
Clinical signs: emphysema

Note: 10 vials of glycerol stored in freezer A and 10 vials of PDAM stored in freezer B
ISR2010/02

BTV-4 

Identified as BTV 4 by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Dorot

Sample taken

8th Jan 2010

Cattle blood

Isolated in
 KC cells at IAH-P

Shelf B17

box  β,  

rows C1-C10

 

Added to collection
8th Apr 2009
3100/09/1 [IAH/KC1]

Jacob Brenner , Kimron Institute Israel

 

Samples arrived 23rd March 2010

IAH Stars
number
A24/10 05
Clinical signs: Emphysema, respiratory distress, abortion

Note: 10 vials of glycerol stored in freezer A and 10 vials of PDAM stored in freezer B
ISR2010/01

BTV-4 

Identified as BTV 4 by Seg-2 specific

 Real time RT-PCR 

Israel,

 from Dvir

Sample taken

4th Jan 2010
Cattle blood

Isolated in
 KC cells at IAH-P

Shelf B17,

box β,

rows A1-A10

Added to collection
8th Apr 2009
1035/10/3 [IAH/KC1]

Jacob Brenner , Kimron Institute Israel

 

Samples arrived 23rd March 2010

IAH Stars
number
A24/10 01

Clinical signs: Subcutaneous emphysema, Died

Note: 10 vials of glycerol stored in freezer A and 10 vials of PDAM stored in freezer B

QTR2010/01

BTV-4 

Identified as BTV-4 by Seg-2 specific

 Real time RT-PCR 

Qatar Shelf B18,
box α,
rows E1-E10

added to collection 25th may 2010
*
Duplicate sample vial position E3 were given to Eva Veronessi (Pirbright) on 26th February 2013
*
Insufficient material to store more samples . All samples stored
with glycerol.
Freezer B only contains
samples E1-E9

*

Vial position E3 was removed from  Freezer A and given to Kyriaki Nomikou (Pirbright) on 19th February 2013.

Equivalent vial  in Freezer B was not removed

*

SPA2010/01

BTV-4

 Identified as BTV-4 by Seg-2 specific

 Real time RT-PCR in Spain

Cadiz Bovine Blood Isolated at LCV Spain

Shelf C12,

box C12 β,

rows I1-J10

 

Added to collection on
2nd Feb 2011
5762/10
sample 1
E1/C6/36-1/ BHK3
 [IAH/BHK1]

Marta Vigo Martin

LCV Algete Spain
(MTA)
2009
ARG2009/01

BTV-4 Identified as BTV-4 by Seg-2 specific

 Real time RT-PCR

and sequen

From Argentina,

Corrientes province.

 

 (blood sample collected

14th June 2007)

Isolated from

Sheep blood

 

Isolated at

Instituto de Virología
INTA Castelar
Buenos Aires. Argentina by Dra. María José Dus Santos

Shelf D8,

box  D8 α,

rows G1-H10

 

(Added to

collection on

5th November 2013)
4/ARG/01/2009

CRL1660 passages2 /BHK3

[TPI/BHK1]

Dra. María José Dus Santos
Instituto de Virología
INTA Castelar
Buenos Aires. Argentina.

Sent
19th Sep 2013
Distribution restricted by MTA
ISR2009/17

BTV4

Identified 

by Seg-2 specific

 Real time RT-PCR  [LSI]

Israel

 From Hadaron region

Tekuma

Sheep sample

1st Oct  2009

Isolated in
 KC cells
 at IAH-P

Shelf B13,

box  B13 beta,

 rows G1-H10

Added to collection on  25th Nov 2009 

3096/09

[IAH/KC1]

Dr. Hagai Yadin, Kimron Institute Israel  

 

Samples arrived 2nd November 2009

 

[IAH Stars No.
A88/09 05]

Outbreak data
MOR2009/15

BTV-4

Confirmed by real time RT-PCR targeting Seg 2

and Seg-2 sequence analyses

Sheep sampled in Marrakech,

 Morocco

on 29th Oct 2009

 Isolated from sheep blood at TPI Pirbright

Shelf D9,

box  D9 gamma,

 rows E1-F10

 

(Added to

collection on

18th Mar 2014)

410/09-1

[TPI/KC1BHK1]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

A94/09 10

Outbreak Data

 

*

Derived from MOR2009/10

  

Distribution restricted
by MTA

MOR2009/14

BTV4

Confirmed by real time

RT-PCR Seg 2

Sheep sampled in Marrakech Morocco on 29/10/09

Ovine blood Isolated at IAH Pirbright

Shelf C16,

box C16 γ,

rows E1-F10

 

(Added to

collection on

16th Dec 2011)

410/09-1

[IAH KC2]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

A94/09 10

Derived from

MOR2009/10

Distribution restricted by MTA

MOR2009/12

BTV4

Confirmed by real time RT-PCR Seg 2
Sheep sampled in Azrou Morocco on 28/10/09 Ovine blood Isolated at IAH Pirbright

Shelf  C15

Box C15γ

Rows G1-H10 

(added to collection on

 14th Oct 2011)

407/09-4 [IAH/KC2]

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

A94/09 07

Derived from MOR2009/07
*
100ml of this passage level sent to Michael Eschbaumer  at FLI Rheims Germany , as a vaccine challenge strain on 13th Oct 2011 under
MTA
*
100ml of this passage level sent to Piet van Rijn CVI at Lelystad the Netherlands, as a vaccine challenge strain under MTA
*

MOR2009/10

BTV4

Confirmed by real time RT-PCR Seg 2

Sheep sampled in Marrakech Morocco on 29/10/09

Ovine blood Isolated at IAH Pirbright

Shelf B14

Box B14 α

Rows I1-J10

 (added to collection on

 1st Dec 2009 )

410/09-1

IAH KC1

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

A94/09 10

(MTA)
*
U
sed to generate MOR2009/13
*
Duplicate vials, positions J1 (p
given to K. Nomikou (IAH-P) 15th Apr 2010
*
Duplicate vials, positions J2 (P)  given to K. Nomikou (IAH-P)  20th May 2010
*
Duplicate vials, positions J3 (P)  given to K. Nomikou (IAH-P)  13th Dec 2010
*
Duplicate vials, position I2 were  taken out of Freezer A and B and given to Eva Veronesi (Pirbright) 26th April 2013
*

Duplicate sample vial position I1 were given to Eva Veronessi (Pirbright) on

26th February 2013

*


Outbreak data
MOR2009/09

BTV4

Confirmed by real time RT-PCR Seg 2
Sheep sampled in Azrou Morocco on 28/10/09 Ovine blood Isolated at IAH Pirbright

 Shelf B14

Box B14 α

Rows G1-H10

 (added to collection on

 1st Dec 2009)

407/09-7 IAH KC1

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

A94/09 09


(MTA)
Outbreak data

Vial position G1 (g) freezer A and B was sent to Javier Ortego Valdeolmos in Madrid
on 15th Dec 2010

*
Vial position G2 (g A +B) used on 21st Feb 2011  to prepare  passage level KC2, then sent to E. Veronesi and G. Venter in  OVI, Onderstepoort South Africa for transmission studies
MOR2009/08

BTV4

Confirmed by real time RT-PCR Seg 2
Sheep sampled in Azrou Morocco on 28/10/09 Ovine blood Isolated at IAH Pirbright

Shelf B14

Box B14 α

Rows E1-F10

 (added to collection on

27th Nov 2009 )

407/09-6 IAH KC1

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

A94/09 08

(MTA)
Outbreak data
MOR2009/07

BTV4

Confirmed by real time RT-PCR Seg 2
Sheep sampled in Azrou Morocco on 28/10/09 Ovine blood Isolated at IAH Pirbright

Shelf B14

Box B14 α

Rows C1-D10

 (added to collection on

 27th Nov 2009 )

407/09-4 IAH KC1

Mehdi El Harrak (Biopharma Laboratory, BP4569, Rabbat Akkari, Morocco

 

A94/09 07

*
Duplicate vial positions C10 (G) sent to Marta Vigo Martin, Algete Spain on 15th Dec 2009 under MTA
*
Duplicate vials, position C1  given to C. Batten (IAH-P)  28th April 2010 for  Ring Trial
*
Used to generate MOR2009/12 as a vaccine challenge strain
*
Duplicate vials position C3  from freezers A and B given to K. Nomikou
(3rd May 2012) to prepare MOR2009/12
*

Duplicate vials, position C4 were  taken out of Freezer A and B and given to Eva Veronesi (Pirbright) 26th April 2013
*

Duplicate sample vial position D1 were given to Eva Veronessi (Pirbright) on

26th February 2013

*

Outbreak data

OMN2009/10

BTV-4

Identified as BTV by Seg-7 specific

 Real time RT-PCR 

Identified as BTV-4 by Seg-2 Real-time RT-PCR

Sohar in Oman

Samples collected
24th May 2009

Fallow deer blood

Isolated in KC cells at IAH-P

Shelf B9,

box B9 β,

rows  I1- J10

Added to collection
17th June 2009
Oman designation:  fallow deer edta [IAH/KC1]

Masha Japhet,

Oman

 

Samples arrived

Approx 15TH May 2009

IAH Stars No.
A46/09 26
not all +ve animals showed clinical signs of disease. Only Wiltshire sheep, Arabian gazelles, fallow deer and blackbucks were affected . Only one multi-horn sheep died, showing similar signs and post mortem changes, but all of the other live animals sampled were -ve. The +ve Cattle, buffalo, goats and reem gazelles showed no signs of disease .
Strains of  BTV-1, BTV-8 and BTV-16 were also isolated in early 2009
 (see: 
OMN Isolates)

OMN2009/09

BTV-4

Identified as BTV by Seg-7 specific

 Real time RT-PCR

Identified as BTV-4 by Seg-2 Real-time RT-PCR  

Sohar in Oman

Samples collected
24th May 2009

gazelle blood

Isolated in KC cells at IAH-P

Shelf B9,

box B9β,

rows G1- H10

Added to collection

17th June 2009

Oman designation: reem gazal EDTA

[IAH/KC1]

Masha Japhet,

Oman

 

Samples arrived

15th May 2009

IAH Stars
number

A46/09 5

not all +ve animals showed clinical signs of disease. Only Wiltshire sheep, Arabian gazelles, fallow deer and blackbucks were affected . Only one multi-horn sheep died, showing similar signs and post mortem changes, but all of the other live animals sampled were -ve. The +ve Cattle, buffalo, goats and reem gazelles showed no signs of disease .
Strains of  BTV-1, BTV-8 and BTV-16 were also isolated in early 2009
(see: 
OMN Isolates)

Vial position  G1 (g) from freezer A  given to Kyriaki Nomikou 24-APR-2012 to prepare  RNA to be sent to Sanger  Centre for sequencing.  The corresponding tube was not removed from freezer B

2008

ISR2008/04

BTV-4

Identified as BTV by Seg-1 specific

 Real time RT-PCR  [3]

Identified as serotype 4  and 24 by Seg-2 specific real time RT-PCR

BTV-24 confirmed by sequence analyses 

Israel,

 from a village called: Enon

samples

24th Nov 2008

Spleen (species not recorded)

 Isolated on eggs in Israel

Shelf B8,

box B8 α,

rows C1-D10

Added to collection on

28th Jan 2009

Kimron Institute No.

593014

E1

[IAH/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars
No. 

A181/08 12]

ISR2008/02

BTV-4 and BTV-24

 

Identified as BTV by Seg-1 specific

 Real time RT-PCR  [3]

 

Identified as serotype 4 by Seg-2 specific real time RT-PCR

Israel,

 from a village called: 

Zde Eliahu

 

samples taken 13th Oct 2008

From cattle blood.

Isolated in chicken eggs in Israel

Shelf B7

box B7γ,

rows I1-J10

Added to collection on

28th Jan 2009

Kimron Institute No.

591360

E1

[IAH/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

 

Samples arrived

Approx 17th Dec 2008

 

[IAH Stars
No. A181/08 2]

2006
ISR2006/12

BTV-4

Identified as BTV by Seg-7 specific

 RT-PCR 

Identified as serotype 4 by Seg-2 specific RT-PCR

Israel,

 from a village called:  Elyakim

samples

17th Nov 2006

Isolated in Israel from Sheep showing clinical signs

 Shelf D5

box D5β,

rows C1-D10

Added to the collection on 2nd Fen 2007

isolate number

532390

(2309)

CE1

[IAH/BHK1]

Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15th Dec 2006

IAH Stars 
number
 R12/06 -1

Outbreak data

  

ISR2006/10

BTV-4

Identified as BTV by Seg-7 specific

 RT-PCR 

Identified as serotype 4 by Seg-2 specific RT-PCR

Israel,

 from a village called:  Alon Hagalil

sampled

1st Nov 2006

Isolated in Israel from Sheep showing clinical signs

Shelf D5,

box D5β,

rows A1-B10

Added 2nd Feb 2007

isolate number 530659

(2231)

CE1

[IAH/BHK1]

Dr. Hagai Yadin, Kimron Institute Israel

Samples arrived

15th Dec 2006

IAH Stars 
number
 R12/06 -3

 

Outbreak data

Original isolate held in misc box

2005

SPA2005/05

BTV-4

Identified as BTV by RT-PCR  and

type 2 by type specific RT-PCR

Spain

Blood sample taken

10th Aug 2005

Virus isolated from cattle  blood sample at IAH Pirbright by Sue Graham

Shelf C3, 
box C3γ.
rows I1-J10
(added to 
collection 
25th Nov 2005)

blood sample number

5372-8

[IAH/E1/BHK2]

Miguel Angel Jiménez-Clavero

Departamento de Enfermedades  Emergentes

[R10/05

- sample 6]

sequence analyses of Seg-2 show that this isolate is closely related to isolates SPA2004/01 SPA2004/02

OIE report -14th Oct 2005

 

Vial position F1G given to C. Batten (IAH Pirbright) 21 Sept 2008 for rimg trial

spare vial  held in misc box

 

[S5: AM778441]

SPA2005/04

BTV-4

Identified as BTV by RT-PCR  and

type 2 by type specific RT-PCR

Spain

Blood sample taken

 10th Aug 2005

Virus isolated from cattle blood sample at IAH Pirbright by Sue Graham

Shelf C3, 
box C3γ.
rows G1-H10
(added to 
collection 
25th Nov 2005)

blood sample number

5372-7

[IAH/E1/BHK2]

Miguel Angel Jiménez-Clavero

Departamento de Enfermedades  Emergentes

[R10/05

- sample 5]

sequence analyses of Seg-2 show that this isolate is closely related to isolates SPA2004/01 and  SPA2004/02

OIE report -14th Oct 2005

 

SPA2005/03

BTV-4

Identified as BTV by RT-PCR  and

type 2 by type specific RT-PCR

Spain

Blood sample taken

 10th Aug 2005

Virus isolated from cattle blood sample at IAH Pirbright by Sue Graham

Shelf C3, 
box C3γ.
rows E1-F10
(added to 
collection 
25th Nov 2005)

blood sample number

5372-6

[IAH/E1/BHK2]

Miguel Angel Jiménez-Clavero

Departamento de Enfermedades  Emergentes

[R10/05

- sample 4 ]

sequence analyses of Seg-2 show that this isolate is closely related to isolates SPA2004/01 and  SPA2004/02

OIE report -

14th Oct 2005

 

SPA2005/02

BTV-4

Identified as BTV by RT-PCR  and

type 2 by type specific RT-PCR

Spain

Blood sample taken

10th Aug 2005

Virus isolated from cattle blood sample at IAH Pirbright by Sue Graham

Shelf C3, 
box C3γ.
rows C1-D10
(added to 
collection 
25th Nov 2005)

blood sample number

5372-5

[IAH/E1/BHK2]

Miguel Angel Jiménez-Clavero

Departamento de Enfermedades  Emergentes

[R10/05

-sample 3]

OIE report - 14th Oct 2005

sequence analyses of Seg-2 show that this isolate is closely related to isolates SPA2004/01 and  SPA2004/02

*

Sample C1 from both freezers given to K. Nomikou (IAH) , for sequencing on 7th July 2011

*

2004

CYP2004/03

BTV Type 4

(Identified as BTV by ELISA & Typed by serum neutralisation and RtPCR)

Ammochostos,

Cyprus

 (12-2-2004)

Sheep (spleen)

 Isolated on 10-3-2004 at: Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou &  O.Mangana

Shelf B3,

box B3β,

rows E1-F10

30th Sept. 2004

CY1035/1/04

CPAE& BHK 21 Cells

 Spleen

 (CPAE1 BHK4)

+[IAH/BHK2]

Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens

It was initially suggested by SNT that this sample might also contain BTV-16, but type specific RT-PCR primers only amplified BTV-4 segment 2

[S3:DQ186799]

MOR2004/02

BTV Type 4

Identified as BTV by Seg-7

RT-PCR,

Typed by

Seg-2 RT-PCR

El Kebir

 (north of Morocco)

Sheep

Shelf B2, boxB2β,

rows C1-D10

(16/Mar/2005)

BTV/KEK

from blood

[IAH-E1/BHK4]

Dr Mehdi El Harrak

Virology department

Biopharma Laboratory

BP4569 Rabat Akkari

Morocco

*

Duplicate samples

(1 /10) 'D1-(PDAM)' from freezers a and b, were given to Lydia Kgosana  (IAH Pirbright) on 13th Jan 2006

*

Duplicate sample vial position C2 were given to Eva Veronessi (Pirbright) on

26th February 2013

*

OIE reports from 15th October

[Seg3:DQ186817]

SPA2004/02

BTV

Type 4

Cadiz province at

Jimena de la Frontera

Sheep

 

(Isolated by Sue Graham,

 IAH Pirbright)

shelf B1,

box B1β

rows C1 - D10

(05/12/2004)

 

Blood

 

[IAH: E1/BHK1]

Concepcion Gomez-Tejedor

Laboratorio Central de Veterinaria

ctra. De Algete km8

28110 Algete (Madrid)

  [R12/04 (no.21)]

 OIE report 15th October 2004
*

Duplicate samples 1 /10 'C2-(g)' from freezers a and b, were given to S. Maan  (IAH-P) on 27th Sept 2006

*

Vial positions  E1 (p)  were given to Eva Veronesi (IAH Pirbright on 13th May 2010 for Ph.D studies).  one of these was retained in Misc box C,  bottom of freezer A
position E6

*

SPA2004/01

BTV

Type 4

Cadiz province at

Jimena de la Frontera

Sheep

 

(Isolated by Sue Graham,

 IAH Pirbright)

shelf B1,

box B1β

rows A1- B10

(05/12/2004)

 

 

blood

[IAH/E1/BHK1]

 

Concepcion Gomez-Tejedor

Laboratorio Central de Veterinaria

ctra. De Algete km8

28110 Algete (Madrid)

[R12/04 (no.4)]

Aliquots 1/10a & 1/10b (p)  given to Adam Meyer (IAH, Pirbright - 19th April 2005)

Aliquots 2/10a & 2/10b (g)  given to Sue Graham

(IAH, Pirbright -

20th May 2005)

Aliquots B3/10a & B3/10b (p)  given to Carrie Batten (IAH, Pirbright - 26th January 2006) 

 OIE report 15th October 2004

2003
COR2003/06 BTV-4

from Corsica

2003

isolated at AFSSA- Maisons Alfort France

in 2003

 Shelf C15,

box C15 Beta,

rows A1– B10

Added to collection

20 September

2011

Original tube marked as: 200 E1/BHK2
[TPI/BHK1
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material Vaccination status of animal unknown
COR2003/05 BTV-4

from Corsica

2003

isolated at AFSSA- Maisons Alfort France

in 2003

Original tube marked as: 217 BHK
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material Vaccination status of animal unknown
COR2003/04 BTV-4

from Corsica

2003

isolated at AFSSA- Maisons Alfort France

in 2003

 Shelf C15,

box C15 Alpha,

rows I1– J10

Added to collection

20  September 2011

Original tube marked as:
 bt4 -03-261
E1/BHK2
[TPI/BHK1]
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material Vaccination status of animal unknown
COR2003/03 BTV-4

from Corsica

2003

isolated at AFSSA- Maisons Alfort France

in 2003

Original tube marked as: 234 BHK
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material Vaccination status of animal unknown
COR2003/02 BTV-4

from Corsica

2003

isolated at AFSSA- Maisons Alfort France

in 2003

 Shelf C15,

box C15 Alpha,

rows G1– H10

Added to collection

20  September 2011

Original tube marked as: 03.124 E1, BHK2
[TPI/BHK1]
Supplied by Emmanuel Breard, AFSSA, Maisons Alfort France 23rd July 2010 as cell culture material Vaccination status of animal unknown

COR2003/01

(FRA2003/01)

BTV-4

from Corsica

2003

isolated at AFSSA- Maisons Alfort France

in 2003

 Shelf E2,

box E2β,

rows C1– D10

added to collection on

13th Sept 2007

03.134 WIB1:

wild BTV-4

E1/BHK1

[IAH/BHK2]

Supplied by Stephan Zientara and Emmanuel Breard,

AFSSA-Maisons Alfort-France

Vaccination status of animal unknown

*

Sample C1 from both freezers given to K. Nomikou (IAH) , for sequencing on 7th July 2011

*

SPA2003/04

BTV

Type 4

(Typed by

Seg-2

RT-PCR & by Serum Neutralisation Test)

Menorca,

Spain

(17/11/03)

Sheep

(25/11/03 isolated from blood by Sue Graham IAH Pirbright)

Shelf A3,

Box A3γ,

Positions

G1-H10

No. 3

[R16/03]

from blood

[IAH-E2/BHK2]

Dr. Ana Canals

Centro de Investigacion en Sanidad Animal 28130 Valdeolmos

ESPANA

Tube H1 (Freezer B)  given to Sue Graham  (Pirbright)  20th May 2005

*

Tubes G1 (freezer A) was  given to S. Maan (Pirbright) 27th Sept 2006

OIE report 19th Dec 2003

Vol. 16 No. 51

SPA2003/03

BTV

Type 4

(Typed by

Seg-2

RT-PCR and sequence analysis & by Serum Neutralisation Test)

Menorca,

Spain

(17/11/03)

Sheep

(25/11/03 isolated from blood by Sue Graham IAH Pirbright)

Shelf A3,

Box A3γ,

Positions

E1-F10

(25/08/2004)

No.36

[R16/03]

from blood

[IAH-E1/BHK3]

Dr. Ana Canals

Centro de Investigacion en Sanidad Animal 28130 Valdeolmos

ESPANA

OIE report 19th Dec 2003

Vol. 16 No. 51

 

SPA2003/02

BTV

Type 4

(Typed by

Seg-2

RT-PCR and sequence analysis & by Serum Neutralisation Test)

Menorca,

Spain

(17/11/2003)

Sheep

(25/11/03 isolated from blood by Sue Graham IAH Pirbright)

Shelf A3,

Box A3γ,

Positions

C1-D10

(25/08/2004)

No.34

[R16/03]

from blood

[IAH-E1/BHK3]

Dr. Ana Canals

Centro de Investigacion en Sanidad Animal 28130 Valdeolmos

ESPANA

Isolate used by Shujing Rao, IAH Pirbright for cDNA synthesis, to send to P.Roy for VLP production (March 2005)

 *

Tubes C1 (freezer A&B) supplied to Adam Meyer (Pirbright) for RNA production for Raniero Lorenzetti (June 2005) 

*

  Tubes D1(freezers A&B) supplied  to S. Maan  (Pirbright) on 27th Sept 2006

*

[S6:AJ783909

S10:AJ783911]

OIE report 19th Dec 2003

Vol. 16 No. 51

SPA2003/01

BTV

Type 4

(Typed by

Seg-2

RT-PCR and sequence analysis & by Serum Neutralisation Test)

Menorca,

Spain

(17/11/2003)

Sheep

(25/11/03 isolated from blood by Sue Graham IAH Pirbright)

Shelf A3,

Box A3γ,  Positions

A1-B10

 (25/08/2004)

No.32

[R16/03]

from blood

[IAH-E1/BHK4]

Dr. Ana Canals

Centro de Investigacion en Sanidad Animal 28130 Valdeolmos

ESPANA

Tubes A1 and B1 from freezer A  were supplied to S. Maan  (Pirbright) on 27th Sept 2006

*

Tubes B1 and B2 from Freezer B were removed from the collection

*

[Seg3:DQ186824 Seg6:AJ783908,

Seg10:AJ783910]

OIE report 19th Dec 2003

Vol. 16 No. 51

2002

ISR2002/11

BTV-4

Israel

Beit Dagan

2002

Cow

 (01/12/2002

at the Kimron Institute)  

Shelf A5,

Box A5α

Rows G1-H10

  (369750

Lab No: 2898)

E1

[IAH-E1/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

(MTA)

ISR2002/10

BTV-4

 Israel ,

Beit Dagan

2002

Cow (06/11/2002 at the Kimron Institute)

 Shelf A5,

Box A5α,

Positions

A1-B10

 (367003

Lab No: 2775)

(E1)

[IAH/E1/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

isolate related to  ISR2002/09

 (MTA)

 >> contents

ISR2002/09

BTV Type 4

Israel,

Beit Dagan

Cow (06/11/2002 at the Kimron Institute)

Shelf B4,

Box B4γ,

Rows I1-J10

Added to collection on 16th June 2005

 (367003

Lab No: 2774)

(E1)

+

[IAH/E1/BHK4]

Dr. Hagai Yadin, Kimron Institute Israel

isolate related to ISR2002/10

 (MTA)

ISR2002/08

BTV Type 4

Israel Beit Dagan

Cow (31/10/2002 at the Kimron Institute)

 (366280

Lab No: 2731)

(CE/ )

Dr. Hagai Yadin, Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

 >> contents

ISR2002/07

BTV Type 4

Israel ,

Beit Dagan

Cow (29/10/2002

at the Kimron Institute)

Shelf A5,

Box A5β, Rows I1-J10

10/Sept/2004

 (366058

Lab No: 2714)

(E1 )

[IAH/E1/BHK2]

Dr. Hagai Yadin, Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source 

ISR2002/06

BTV Type 4

Israel,

 Beit Dagan

Cow (24/10/2002

at the Kimron Institute)

Shelf B4,

box B4β,

Rows G1-H10

 (365657

Lab No: 2693)

(E1 )

[IAH/E1/BHK4]

Dr. Hagai Yadin,

Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

 >> contents

ISR2002/05

BTV- 4

Israel ,

Alon Ha-Ggalil 

2002

Sheep (22/10/2002

at the Kimron Institute) 

Shelf A5,

Box A5β, Rows G1 - H10

10/Sept/2004

(365373  

Lab No: 2684)

(E1)

[IAH/E1/BHK2]

Dr. Hagai Yadin,

 Kimron Institute Israel

isolate related to ISR2002/04

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source 

ISR2002/04

BTV Type 4

Israel,

 Alon

Ha-Ggalil

Sheep (22/10/2002

at the Kimron Institute)  

Shelf B3,

box B3β

 row G1-H10

 added to collection on 09/05/2005

(365373  

Lab No: 2683)

(E1 ) 

+

[IAH/BHK2]

Dr. Hagai Yadin,

Kimron Institute Israel

isolate related to ISR2002/05

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

 >> contents

ISR2002/03

BTV-4

Israel,

 Beit Dagan

2002

Cow (21/10/2002 at the Kimron Institute)

Shelf A5, boxA5β,

rows G1-H10 

 (365226

Lab No: 2671)

 (CE/ )

Dr. Hagai Yadin,

Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

ISR2002/02

BTV-4

Israel

Kabul

2002

Sheep (15/10/2002 at the Kimron Institute)

 Shelf A5,

Box A5α,

Positions

C1-D10

 (364459

Lab No: 2647)

(E1 )

[IAH/E1/BHK2]

Dr. Hagai Yadin,

Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

 >> contents

ISR2002/01

BTV Type 4

Israel

Beit Dagan

2002

Cow (06/10/2002 at the Kimron Institute)

 (363238

Lab No: 2582)

(CE/ )

Dr. Hagai Yadin,

Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

ARG2002/04

BTV Type 4

Argentina

sample taken  21st Feb 2002

Bovines   

Shelf C4, 
box C4β
rows A1 - B10
(added to 
collection
 19th Dec 2005)

[R4/02

(no 102)]

adapted to

BHK cells

 E2/V2/ BHK3

[IAH/BHK2]

Irene Lager (June 2002)

ARG2002/03

BTV Type 4

Argentina 21/02/02

Bovines

Shelf C4, 
box C4γ
rows I1 - J10
(added to 
collection
  8th Dec 2005)

[R4/02 (no 99)]

Adapted to BHK cells

E2/V2/ BHK4

[IAH/BHK2]

Irene Lager (June 2002)

 >> contents

ARG2002/02

BTV

Type 4

Argentina 21/02/02

Bovines

Shelf C4, 
box C4γ
rows G1 - H10
(added to 
collection
  8th Dec 2005)

[R4/02 (no 82)]

Adapted to BHK cells

 E2/V2/ BHK5

[IAH/BHK2]

Irene Lager (June 2002)  

Vial position H1 was removed from  Freezer A and given to Kyriaki Nomikou (Pirbright) on 19th February 2013.

Equivalent vial  in Freezer B was not removed

ARG2002/01

BTV Type 4

Argentina 21/02/02

Bovines

Shelf D8,

box D8 α,

rows A1-B10

 

Added to collection

15th Mar 2013

[R4/02 (No 20)]

BHK

[E2/V2/ BHK2]

Irene Lager (June 2002)    

 >> contents

[S2: S6:AJ586682]

2001
ARG2001/03

BTV-4 

(Type confirmed by SNT, Seg-2 RT-PCR and sequencing)

From Argentina,

Corrientes province ,

Santo Tome.

 (blood sample collected

21st February 2002)

Isolatedfrom bovine blood

 

Isolated at

Instituto de Virología
INTA Castelar
Buenos Aires. Argentina.
by Irene Lager

 

 

27th Nov 2008

Shelf D8,

box D8 beta

Rows E1-F10

 

(Added to

collection on

5th Nov 2013)
4/ARG/829/2001 E2/V2/BHK5
[TPI/BHK1]

Dra. María José Dus Santos
Instituto de Virología
INTA Castelar
Buenos Aires. Argentina.

Sent 19-09-2013
Distribution restricted by MTA
ARG2001/02

BTV-4 

(Type confirmed by SNT, Seg-2 RT-PCR and sequencing)

From Argentina,

Corrientes province ,

Santo Tome.

 (blood sample collected

21st February 2002)

Isolated from bovine blood

 

Isolated at

Instituto de Virología
INTA Castelar
Buenos Aires. Argentina.
by Irene Lager

  

27th Nov 2008

Shelf D8,

box D8 beta

Rows C1-D10

 

(Added to

collection on

5th Nov 2013)
4/ARG/102/2001 E2/V2/BHK3
[TPI/BHK1]

Dra. María José Dus Santos
Instituto de Virología
INTA Castelar
Buenos Aires. Argentina.

Sent

 19th Sep 2013

Distribution restricted
by MTA

ARG2001/01

BTV-4 

(Type confirmed by SNT, Seg-2 RT-PCR and sequencing)

From Argentina,

Corrientes province ,

Santo Tome.

 (blood sample collected

21st Feb 2002)

Isolated from bovine blood

 

Isolated at

Instituto de Virología
INTA Castelar
Buenos Aires. Argentina.
by Irene Lager

  

27th Nov 2008

Shelf D8,

box D8 beta

Rows A1-B10

 

(Added to

collection on

5th Nov 2013)

4/ARG/99/2001

E2/V2/BHK4
[TPI/BHK1]

Dra. María José Dus Santos
Instituto de Virología
INTA Castelar
Buenos Aires. Argentina.

Sent

 19th Sep 2013

Distribution restricted

by MTA

ISR2001/13

BTV Type 4

Israel,

 kfar Salad

sheep (22/11/2001 at the Kimron Institute)

Shelf B4,

box B4α

Rows C1-D10

 

(added to collection on

27th May 2005)

 

(326038

Lab No: 8244) )

(E1 )

[IAH/E1/BHK4]

 Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source
 

 >> contents

ISR2001/12

BTV Type 4

Israel,

 kfar Shmuel

Sheep

 (24/10/2001

at the Kimron Institute)

Shelf B4,

box B4β,

Rows I1-J10

(323282

Lab No: 8063)

 (E1 )

 

[IAH/E1/BHK4]

Dr. Hagai Yadin, Kimron Institute Israel

isolate related to ISR2001/11

 

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

ISR2001/11

BTV Type 4

Israel,

 kfar Shmuel

Sheep (24/10/2001 at the Kimron Institute)

Shelf B4,

box B4α

Rows E1-F10

 

(added to collection on

27th May 2005)

(323282

Lab No: 8062)

(CE1 )

 

[IAH/E1/BHK4]

Dr. Hagai Yadin, Kimron Institute Israel

isolate related to ISR2001/12

 

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source
 

 >> contents

ISR2001/10

BTV Type 4

Israel,

 Givat Ishhihu

 

2001

 Sheep (22/10/2001 at the Kimron Institute)

Shelf A5,

Box A5γ, RowsA1-B10

 

10/Sept/2004 

(323033

Lab No: 8042)

 (E1 )

 

[IAH/E1/BHK]

Dr. Hagai Yadin, Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

ISR2001/09

BTV Type 4

Israel,

 Givat Ishhihu

 

2001

Sheep (22/10/2001

at the Kimron Institute)

 Shelf A4,

Box A4γ,

Positions

G1-H10

(323033

Lab No: 8041)

 (E1)

 

[IAH/E1/BHK2]

 

Dr. Hagai Yadin,

Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source
 

 >> contents

 ISR2001/08

BTV Type 4

Israel,

 Givat Ishhihu

 Sheep (22/10/2001 at the Kimron Institute)

 

(323033

Lab No: 8040)

(CE/ )

Dr. Hagai Yadin,

Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

ISR2001/07

BTV-4

Israel,

 Givat Ishhihu

2001

Sheep (22/10/2001 at the Kimron Institute)

 

Shelf A4,

Box A4γ, Positions

E1-F10

 (323033

Lab No: 8039)

 (E1)

[IAH/E1/BHK2]

Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

 >> contents

ISR2001/06

BTV Type 4

Israel,

 Nahalal

Sheep (15/10/2001

at the Kimron Institute)

 

 (322222

 Lab No: 7967)

(CE/ )

Dr. Hagai Yadin,

Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

 ISR2001/05

BTV-4

Israel,

Hatzav

 

2001

Sheep (28/09/2001 at the Kimron Institute)

Shelf A5,

Box A5β, RowsE1-F10

 

10/Sept/2004

(320226

Lab No: 7782)

(E1)

[IAH/E1BHK2]

Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source
 

 >> contents

ISR2001/04

BTV-4

Israel,

 Hatzav

Sheep (28/09/2001 at the Kimron Institute)

 

 

 

(320226

Lab No: 7781)

(CE/ )

Dr. Hagai Yadin, Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

ISR2001/03

BTV-4

Israel,

 kfar Baruth

 

2001

Sheep

 

 (05/11/2001

 at the Kimron Institute)

Shelf A5,

Box A5α

Rows E1-F10

(324382

Lab No: 8128)

E1

[IAH-E1/ BHK2]

Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source
 

 >> contents

 ISR2001/02

BTV Type 4

Israel,

 kfar Salad

Goat (12/11/2001 at the Kimron Institute)

 

(325160

Lab No: 8176)

 

 (CE/ )

Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source

ISR2001/01

BTV Type 4

Israel,

 Bat Slomo

Sheep (05/09/2001 at the Kimron Institute)

 Shelf A5,

Box A5α,

Rows I1-J10

 (318830

Lab No: 7678)

E1

[IAH-E1/BHK2]

Dr. Hagai Yadin,

 Kimron Institute Israel

*MTA* , not to be supplied 
 to 3rd parties without written 
consent from original source
 

 >> contents 

[Seg3:DQ186813]

2000
GRE2000/08

 

BTV Type 4

 

identified as BTV by ELISA and Seg-7 RT-PCR 

 :

 Typed by serum neutralisation assay

GREECE , Kavala

(North Greece)

 

(sample taken 13th April 2000)

Isolated from

adult Culicoides obsoletus 

 

 At : Centre of Athens Vet. Institutions, Virus Lab, Athens.

 

 By:

K. Nomikou

Shelf A12

Box A12 α,

A1-B10

 

Added to collection

14th July 2008

 

from C. Obsoletus : adapted to BHK 21 CELLS

 

 

 

BHK6

[+IAH/BHK1]

Nomikou Kiki

 

 Centre of Athens

Vet. Institutions,

Virus Lab,

 Athens

 

 

 

GRE2000/07

BTV Type 4

 

(Identified as BTV by  ELISA , Serum Neutralisation Test &  RT-PCR.

Serotyped by Serum Neutralisation Test)

 

Greece,

 Arta

(W Central)

 

 Animal code:7

 

(39o 20΄ Ν,

21o 11΄Ε)

 

(3/Oct/2000)

From sheep

 

(Isolated on 13-10-2000

At : Centre of Athens Vet. Institutions,

Virus Lab.

 

By: Drs. Nomikou K.  &

Mangana O.)

Shelf A2,

Box A2γ,

RowsC1-D10

 

(20/May/2003

GR-1217/00 ArS (7)

BHK 21 cells

(from blood)

 

E2/BHK4

[IAH BHK1]

 

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

 

Seg-10 was sequenced at E2/BHK6

 

Tubes D1 removed

from freezers A & B

 

Tubes D2 (from freezers A & B)given to T. Jabbar, Pirbright on 25th Jan 2008

 

[Seg3: DQ186806

Seg10:AY691692]

GRE2000/06

 

 

BTV-4

Greece

 

 27th Feb 2000

NK   

Shelf A3,

Box A3α, Positions

 A1-B10

 ( 384/99 )

(E2/BHK6)

 

Tubes A1 & B1 (freezer A& B)  supplied to  S. Anthony & H. Jones,  Pirbright 7th June 2004

 

[S10:    ]

GRE2000/05

BTV Type 4

Greece (24/02/00)

Goat

Shelf A2,

Box A2β, Rows I1-J10

 

(01/Apr/2004)

(228/99 )

(E1/BHK5)

 

 

 >> contents 

GRE2000/04

BTV-4

Greece

 

24th Feb 2000

NK   

Shelf A2,

Box A2γ,

 Rows E1-F10

 

Added to collection

1st Apr 2004

 (347/99 )

(E2/V4/BHK2)

 

 

 [S10: ]

GRE2000/03

BTV Type 4

Greece

 

24th Feb 2000

 Bovine

Shelf A3,

Box A3α,

Positions

 C1-D10

 (429/99 )

(E2 /V4/BHK3)

 

Tubes C1 & D1  freezer A & B) supplied to S. Anthony &

H. Jones, Pirbright 07-06-2004

 

 >> contents

GRE2000/01

BTV Type 4

Greece (24/02/00)

Sheep  

shelf B5,

box B5γ,

rows E1-F10

 

(added to collection on 16/08/2005)

(180/99 )  

(E1/BHK4)

[IAH/BHK1]

 

 

[Seg-6:AJ586681,

 Seg-8: AM900372]

1999

GRE1999/29

BTV4

Identified by BTV group-specific Seg-7 RT-PCR and

Real time RT-PCR  [3]

 

Typed  by SNT,

conventional RT-PCR and specific real-time PCR 

GREECE

Lesbos

Aghia

Paraskevi

( Central island)                                   

 

39° 25' N

26°  27'  E

(25th  Nov 1999)
Cattle
 (spleen )                        Isolated at: Centre of Athens Vet. Institutions,    Virus Lab, Athens by: Nomikou K.)

Shelf A12,

Box A12 beta,

rows C1 to D10

&

Shelf B14,

box B14β ,

rows C1-D10

 

Added

to collection

9th Dec 2009

GR 732/99LB

From cattle spleen

adapted to BHK 21 cells

 

 

 

  ( BHK6 )

 [IAH BHK1]

Nomikou Kiki

 

 Centre of Athens Vet. Institutions, Virus Lab, Athens

Animal in the same herd as the
GRE1999/31

GRE1999/27

BTV Type 4

 

(identified as BTV by ELISA and Seg-7 RT-PCR  :

 Typed by serum neutralisation assay)

Greece , Chalkidiki,

Develiki

Lerissos

(North)

 

(sample taken 21st Sept 1999)

Sheep (blood)

 

Virus isolated at the Centre of Athens Vet institutions, virus lab, by K. Nomikou and

O. Mangana

Shelf A11,

box A11α ,

rows I1-J10

 

Added to collection 

10th July 2008  

Animal code 6

Blood

to BHK 21 CELLS

 

 

 

E1 BHK 4

[+IAH/BHK1]

Nomikou Kiki

 

 Centre of Athens Vet. Institutions, Virus Lab, Athens

 

 

GRE1999/26

BTV Type 4

 

(identified as BTV by ELISA and Seg-7 RT-PCR  :

 Typed by serum neutralisation assay)

GREECE , Magnesia , Keramidi 

(Mainland Greece

 39° 32' 230''N

22° 56' 102'' E)

 

( sample taken 15th Oct 1999)

Isolated from

adult Culicoides obsoletus 

 

 At : Centre of Athens Vet. Institutions, Virus Lab, Athens.

 

 By: Dr. K.Nomikou

Shelf A11,

box A11α ,

rows G1-H10

 

(Added to collection

10th July 2008)

 

from C. Obsoletus : adapted to BHK 21 CELLS

 

 

 

BHK5

[+IAH/BHK1]

Nomikou Kiki

 

 Centre of Athens Vet. Institutions, Virus Lab, Athens

 

 

GRE1999/25

BTV Type 4

Greece,

Evros

 ( Doriskos - Feres)

 

(41º 15΄Ν,

26º 19 ΄ Ε)

 

(Original sampling date:  10/08/1999)

 Goat

(blood)

Shelf B4,

box B4α

Rows G1-H10

 

(added to collection on

January 2003)

 

4. F4. GR228/99EG

 

From Blood (56474)

 

 E2/BHK4

 

[IAH/BHK2]

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

Seg-10 sequenced at

E2BHK7

 

Similar sample to GRE1999/07

 

Insects caught at time of original sample :  C.obsoletus

 

Original host was sick but 0% mortality, 0% morbidity

 >> contents

 

[Seg10:AY677622]

GRE1999/24

BTV Type 4

Greece,

 Rodos

 

(36º 17΄Ν,

27º 51΄ Ε)

 

 (1999)

Sheep (blood)

Shelf B2, boxB2β,

rows A1-B10

 

 (January 2003)

(GR-180/99RS)

(E2/BHK4)

[IAH-BHK2]

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

Seg-10 sequenced at

E2/BHK7

 

Similar isolate to GRE1999/01

 >> contents

 

 

[Seg10:AY677623]

 

GRE1999/23

BTV Type 4

Greece, 

Lesbos

 

(39º 20΄Ν,

26º 16 ΄ Ε)

 

(1999)

Sheep

 

(blood)

Shelf C1, 
box C1α,
rows A1-B10
 
Added to 
collection
14th Sept 2005 

 (GR-413/99LS)

E2/ BHK5

[IAH-BHK1]

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

 

(supplied October 2004)

Seg-10 sequenced at

E2/BHK8

 

 Similar sample to

GRE1999/03

[Seg10:AY677626]

GRE1999/22

BTV Type 4

Greece, 

Magnesia

 

(39º 10΄Ν,

23º 27΄ Ε)

 

 (1999)

Sheep

 

(blood)  

shelf B5,

box B5α

rows E1-F10

 

(received October 2004

(GR-631/99Mas)

(E2/BHK7)

 

[IAH/BHK2]

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

Seg-10 sequenced at E2/BHK11

 

similar sample to GRE1999/04

 >> contents

 

[Seg10:AY449656]

GRE1999/21

BTV

Type 4

Greece,

Rodopi

 

(41º 14΄Ν,

25º 53΄Ε)

 

 (1999)

 Sheep

(spleen)

shelf B5,

box B5α

rows C1-D10

 

(received October 2004

(GR-280/99Ros)

E2/BHK9

[IAH/BHK2]

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

Seg-10 sequenced at E2/BHK13

 

 Similar sample to GRE1999/08

 [Seg10:AY691691]

GRE1999/20

BTV

Type 4

Greece,

 Chios

 

(38º 22΄Ν,

26º 08΄ Ε)

 

(1999)

Cattle

 

(blood)

Shelf B4,

Box B4γ.

Rows A1-B10

 

(October

 2004)

(GR-378/99CB)

E2/BHK7

[IAH/BHK1]

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

Sreg-10 sequenced at E2/BHK9

 

Similar sample to

GRE1999/09

  >> contents

 

[Seg10:AY677620]

GRE1999/19

BTV Type 4

Greece,

Lesbos

 

(39º 15΄Ν,

26º 16 ΄ Ε)

 

 (1999)

Sheep

(spleen)

Shelf B6, 
box B6γ,
rows I1-J10
 
Added to 
collection 
7th Sept 2005

(GR-382/99LS)

[IAH-BHK5]

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

 

Supplied October 2004)

similar to sample

GRE1999/02

 >> contents

 

 

GRE1999/18

BTV Type 4

 

(identified as BTV by ELISA & RT-PCR,  Typed by neutralisation assay)

Aghia Paraskevi,

Levos

Greece (NE)

 

(39º 15΄Ν,

26º 16΄ Ε)

 

 (17 Sept. 1999)

sheep (spleen)

 

 At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana

Shelf B1,

box, B1γ,

rows C1-D10

 

30th Sept. 2004

GR382/99LS

BHK 21 cells

 

 spleen

 

(E2BHK7)

 

[IAH/BHK2]

Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.

Seg10 sequenced at E2/BHK10

[S10:AY677624]

GRE1999/17

BTV Type 4

 

(identified as BTV by ELISA and RT-PCR & Typed by neutralisation assay)

N.Pori,

Pieria,

Greece (N.Central)

 

(39º 57΄Ν,

22º 34 ΄ Ε)

 

6th Oct. 1999

From sheep ( spleen) At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana

Shelf B1,

Box B1β,

Rows I1-J10

 

30th Sept. 2004

GR457/99PS

BHK 21 CELLS

 

 spleen

 

 E2BHK7

[IAH/BHK2]

Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.

Seg-10 sequenced at E2/BHK11

[Seg10:AY449655]

GRE1999/16

BTV Type 4

 

(identified as BTV by ELISA and RT-PCR & Typed by neutralisation assay)

Psachna,

Evia,

Greece (Central)

 

(38º 34΄Ν,

23º 38 ΄ Ε)

 

8th Nov.1999

From sheep ( spleen) At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou and O.Mangana

Shelf B3,

box B3β,

rowI1-J10

 

30th Sept. 2004

GR610/99EvS

BHK 21 cells

 

spleen

 

(E2BHK7)

+

[IAH/BHK2]

Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.

Seg10 sequenced at E2/BHK10

[Seg10:AY677627]

GRE1999/15

BTV Type 4

 

(identified as BTV by ELISA and RT-PCR & Typed by neutralisation assay)

Kerasia-Kechros- Sapes,

Rodopi,

Greece

(N. Central)

 

(41º 10΄Ν,

25º 16΄ Ε)

 

13th Aug. 1999

sheep (spleen)

 

 At : Centre of Athens Vet. Institutions, Virus Lab, Athens. By:Dr. K.Nomikou & O.Mangana

 

Shelf B2, boxB2α,

rows E1-F10

 

(30th Sept 2004)

GR279/RoS

BHK 21

 

spleen

 

(E2BHK6)

[IAH-BHK8]

Nomikou Kiki Centre of Athens Vet. Institutions, Virus Lab, Athens.

Seg-10 sequenced at E2/BHK15

 

[S3:DQ186809

S5: AM778440,

S10:AY677621]

GRE1999/14

BTV Type 4

 

(identified as BTV by ELISA and RT-PCR & Typed by neutralisation assay)

Kapi, Pelopi

Lesvos,

Greece (NE)

 

(39º 20΄Ν,

26º 16΄ Ε)

 

(27th Sept. 1999)

sheep (blood)

 

 At : Centre of Athens Vet. Institutions, Virus Lab, Athens.

 

 By:Dr. K.Nomikou & O.Mangana

Shelf B4,

Box B4γ.

Rows C1-D10

 

 

30 Sept. 2004

GR413/99LS

BHK 21 CELLS

 

 blood

 

 (E2BHK5)

+

[IAH/BHK2]

Nomikou Kiki

 

 Centre of Athens Vet. Institutions, Virus Lab, Athens

 

[S10: ]

GRE1999/11

BTV Type 4

 

(Identified as BTV by  ELISA , Serum Neutralisation Test &  RT-PCR.

Serotyped by Serum Neutralisation Test)

Greece

Evia,

Limni-Kirinthos

 (Central)

 

(38º 34΄Ν,

23º 38΄Ε)

 

 (22/Nov/1999)

From sheep

 (spleen)

 

(3/Mar/2000 At:Centre of Athens Vet. Institutions, Virus Lab. By: Dr. Nomikou K. & O.Mangana)

Shelf A3,

Box A3α,

Positions

E1-F10

 

(20/May/2003)

(GR692/99EvS)

BHK 21 cells

 spleen

 

 ( E2/BHK6 )

[IAH-BHK2]

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

Tubes E1  & F1 (freezer a & B) supplied to S. Anthony & H. Jones, Pirbright 7th June 2004

 

Tube F2  freezer A& B was removed from collection

 

  >> contents

 

Seg-10 sequenced at E2/BHK9[Seg10:AY449654]

GRE1999/10

BTV Type 4

 

(Identified as BTV by  ELISA , Serum Neutralisation Test &  RT-PCR.

Serotyped by Serum Neutralisation Test)

Greece,

Lesbos

Kalloni

(NE)

 

(39º 15΄Ν,

26º 13΄Ε)

 

(24 Sept1999)

Sheep

(spleen)

 

(01/ Mar/ 2000

At: Centre of Athens Vet Institutions,

Virus Lab. By:

K. Nomikou

&

O.Mangana )

Shelf A3,

Box A3α,

Positions

G1-H10

 

(20/May/2003)

(GR395/99LS)

BHK 21 cells

 spleen

 

 ( E2/BHK1 )

 

[IAH-BHK1]

Dr. Nomikou Kiki

 

Centre of Athens Vet. Institutions , Virus Lab. Athens

Tubes G1 & H1 (freezer a & B) supplied to  S. Anthony & H. Jones, Pirbright 7th June 2004

 

Tube H2  freezer A& B was removed from collection

 

  >> contents

 

Seg-10 sequenced at E2/BHK3[Seg3:DQ186804

Seg10:AY449653]

GRE1999/09

BTV Type 4

Greece,

 Chios

 

(38º 22΄Ν,

26º 08΄Ε)

 

(1999)

Cattle

 

(blood)

Shelf A3,

Box A3α ,

Rows F1-I10

(January 2003)

(GR-378/99CB)

 

Dr. Charalabos Billinis Aristotle University Thessaloniki

Similar sample to

GRE1999/20

  >> contents

 

[S3: DQ186803

S10:    ]

GRE1999/08

BTV-4

Greece,

Rodopi 

(41º 14΄Ν,

25º 53΄Ε)

 

 (1999)

 Sheep

(spleen)

Shelf A5,

Box A5γ,

positions

 I1-J10

 

(January 2003)

(GR-280/99Ros)

E1/BHK10

[IAH-BHK2]

Dr. Charalabos Billinis Aristotle University Thessaloniki

Similar sample to GRE1999/21

 

 [S10: ]

GRE1999/07

BTV Type 4

Greece,

Evros

 

(41º 15΄Ν,

26º 19΄ Ε)

 

(1999)

Goat

(blood)

Shelf A5,

Box A5β, Rows C1-D10

 

(January 2003)

  (GR-228/99EG) 

E1/BHK6

 Dr. Charalabos Billinis Aristotle University Thessaloniki

Similar sample to GRE1999/25

 >> contents

 

[S10:

S3: DQ186802 ]

GRE1999/05

BTV Type 4

Greece,

 Pieria

 

(39º 57΄Ν,

22º 34΄Ε)

 

 (1999)

Sheep

(spleen)

Shelf A1,

Box A1γ, Positions

A1-B10

 

 (January 2003)

(GR-457/99PS)

[IAH - BHK2]

Dr. Charalabos  Billinis Aristotle University Thessaloniki  

Vials A1 & B1 from freezer A removed from the collection

 

[S10: ]

 

GRE1999/04

BTV-4

Greece,  

Magnesia

 

(39º 10΄Ν,

23º 27΄Ε)

 

 (1999)

Sheep

 

(blood)  

Shelf A6,

Box A6α,

positions

A1-B10

 

(January 2003) 

(GR-631/99Mas)

[IAH-BHK3]

Dr. Charalabos Billinis Aristotle University Thessaloniki

Similar samples to GRE1999/22

 

 >> contents

 

[S3: DQ186801]

GRE1999/03

BTV-4

Greece,    Lesbos

 

(39º 20΄Ν,

26º 15΄Ε)

 

(1999)

Sheep

 

(blood)

Shelf A2,

Box A2γ, Rows A1-B10

 

(January 2003)

 (GR-413/99LS)

[IAH-BHK7]

Dr. Charalabos Billinis, Aristotle University Thessaloniki

Tubes A1 (from freezer A and B),  B1 (from freezer B) & B2 (from freezer A) supplied to

S. Anthony & H. Jones, Pirbright

 7th June 2004

 

similar sample to GRE1999/23

[S10: ]

GRE1999/02

BTV Type 4

Greece,

lesbos

 

(39º 15΄Ν,

26º 16΄Ε)

 

 (1999)

Sheep

(spleen)

Shelf A1,

Box A1γ: Positions

C1-D10

(January 2003)

(GR-382/99LS)

[IAH-BHK5]

Dr. Charalabos Billinis, Aristotle University Thessaloniki

Vial C1 from freezer A removed from collection

 

Similar to sample GRE1999/19

 

 >> contents

 

[S10: ]

GRE1999/01

BTV Type 4

Greece,

 Rodos

 

(36º 17΄Ν,

27º 51΄Ε)

 

 (1999)

Sheep (blood)

 

 

Shelf A3,

Box A3β: Positions

F1-I10

 

 (January 2003)

(GR-180/99RS)

(E1/BHK5)

Dr. Charalabos Billinis Aristotle University Thessaloniki

 Similar to isolate  GRE1999/24

 >> contents

 

 [S10:

S3: DQ186800 ]

1985
TUR1985/01 BTV Type 4 (1985) Virus isolate Shelf D8 ,
box D8γ,
  rows G1 to H10
     
1983
SUD1983/03 BTV-4

Shambat, Sudan

Isolated from bovine blood (Sentinel herd)

Shelf B11,

box  B11 β,

rows I1-J10

 

Added to collection 
21st Oct 2009
NY73

E2/BHK3

M.E.H. Mohammed from same sample as
SUD1983/01

Note:
Sample was originally supplied as untyped EHDV.
However, subsequent work has identified this as an isolate of
BTV-4
*

Vial position J1 was removed from  Freezer A and given to Kyriaki Nomikou (Pirbright) on 19th February 2013.

Equivalent vial  in Freezer B was not removed

*

SUD1983/01

BTV Type 4

Sudan (11/10/83)

NK

  shelf B1,

box B1α

rows G1-H10

 

(02/Feb/2005)

(NY-73)

(E2/BHK1)

[IAH: BHK1]

M.E.H. Mohammed

From same sample as SUD1983/03

 >> contents

 

[S2:AJ585166,

S3:DQ186825,

S6:AJ586677]

 

Note: Sample was originally supplied as untyped EHDV.
However, subsequent (sequencing ) work identified this as an isolate of BTV-4

1980

TUR1980/01

BTV Type 4

Turkey (30/07/80)

NK    

Shelf D8,

 box D8γ,

row I1 to J10

(Aydin virus)

(BHK3)

 

[S10: ]

1979

GRE1979/01

BTV Type 4

Identified by virus neutralisation test only

Madamados,

 (NE) Levos,

Greece

 (NE)

 

(39º 17΄Ν,

26º 20΄Ε)

 

(1979)

From sheep

 (spleen)

 

Isolated at : Centre of Athens Vet. Institutions, Virus Lab, Athens.

By:

Dr. O.Mangana

Shelf B3,

box B3α

rows E1-F10

 

Added to collection

30 Sept 2004

GR79LS

BHK 21 cells

 

from sheep spleen

 

(E2/BHK6)

+

[IAH/BHK3]

Nomikou Kiki

Centre of Athens Vet. Institutions, Virus Lab, Athens

 

Seg-10 sequenced at E2/BHK10

[Seg3:DQ186823

Seg10:AY449650]

1978

TUR1978/01

BTV Type 4

Turkey (00/08/78)

NK    

 

 

(E1/BHK5)

 

 

 >> contents

 

[S2: AJ585165,

S6:AJ586679 S8: AM900373]

 

1977
EGY1977/01       Shelf D8,
Box D8 gamma,
Rows E1 to F10
       
1971

RSA1971/01

BTV-4

 

confirmed by serology

Grown at IAH in Dorset Poll sheep from a sample of virus  originally isolated between

1968-1972

 

 

(3050)

no passage in cell culture

This virus has been passed 4 times in sheep

It has been grown in Eggs and the last two sheep passes were infected with freeze dried blood plus infected egg material  

Freeze dried Virus in sheep blood

(Dorset Poll)

1969

CYP1969/01

BTV Type 4

Cyprus

 

 (1969)

Cow

(Cow tag no A)

Shelf C18

Box C18α

Rows

I1-J10

 

(Added to

collection on

25th May 2012)

 

(IAH/ BHK8)

 

 

[ S6:AJ586678]

 

Sellers et al 1979

 

Distribution restricted by MTA*

 

References
 1.           

Prasad G, Garg AK, Minakshi , Kakker NK, Srivastava RN.(1994) Isolation of bluetongue virus from sheep in Rajasthan State, India.  Rev Sci Tech. 13, 935-938

2.

Mertens PP, Maan NS, Prasad G, Samuel AR, Shaw AE, Potgieter AC, Anthony SJ, Maan S. (2007 ) Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains. J Gen Virol. 88, 2811-2823.

 

3.

Shaw AE, Monaghan P, Alpar HO, Anthony S, Darpel KE, Batten CA, Guercio A, Alimena G, Vitale M, Bankowska K, Carpenter S, Jones H, Oura CA, King DP, Elliott H, Mellor PS, Mertens PP. (2007 Development and initial evaluation of a real-time RT-PCR assay  to detect bluetongue virus genome segment 1.) J Virol Methods.145, 115-126.

 

4.

 

Maan S, Maan NS, Samuel AR, Rao S, Attoui H, Mertens PPC (2007) Analysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes. J Gen Virol. 88, 621-630

 

5.

BLUETONGUE IN SPAIN in the Balearic islands (follow-up report No. 5) OIE report 19th Dec 2003  Vol. 16 No. 51

 

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