|
Family:
Reoviridae, Genus: Orbivirus, Species:
Bluetongue virus, serotype 3
(BTV-3) |
|
*IAH
dsRNA virus collection number
( ICTVdb
isolate Accession Number) |
Virus Species
&
Serotype
(identification method) |
Place of original sample eg country
town [grid reference]
(date
original sample was taken) |
Isolated from which host species e.g
cow, sheep, etc
(Date of virus isolation, where, by
whom) |
Position of storage in reference
freezers
(Date added
to collection) |
Suppliers
Isolate Designation |
Original sample material,
Adapted
to which cell types
(Passage history of sample)
[IAH passage history] |
* Original sample supplied to the collection by person / institution
[IAH, ISIS sample number] |
Comments
References & accession numbers
|
|
RSArrrr/03
|
BTV-3
'Western'
topotype
|
|
NK |
Shelf B2, boxB2α,
rowsC1-D10
(16/Mar/2005) |
(BTV-3
Reference Strain) |
E1/BHK4
[IAH-BHK2] |
Supplied by OVI
S. Africa*
(07/10/83)
Duplicate samples
C1(g) supplied on 19th Dec 2007, to JongChul, Jeong Ja Yeon Agrotech Co., Ltd., 385-6,
ChonChon-Dong,JangAn-Gu, SuWon-City, KyungKi-Do, 440-330, Korea.
Vial D1 (p)
A&B sent to Mehdi El Harrak, Biopharma , Morocco 29th June 2009
*
Vial D2 sent to
Denis Kolbasov, Russian National
Institute of Veterinary Virology 29th
Sept 2009
*
Vial C sent to Dr Graham Abdeljelil,
Institut Pasteur de Tunis 8th
March 2011
*
|
[Seg-1:
KP820919,
Seg-2: KP821039,
Seg-3:
KP821161,
Seg-4:
KP821281,
Seg-5:
KP821401,
Seg-6:
KP821521,
Seg-7:
KP821643,
Seg-8:
KP821763,
Seg-9:
KP821883,
Seg-10:
KP822004,
Nomikou et
al 2015]
[S2:AJ585124,
S6:AJ586697,
S10:
Maan et al 2004 (1),
Singh et al 2004 (2),
Maan et al 2007 (3)]
» contents |
|
USA2007/01
|
BTV- 24
Virus serotype
identified by RT-PCR using type
specific primers and sequencing (Seg-2)
[1]. |
USA: Marion
County Florida
Sample taken
25th Sept 2007 |
White tailed
deer (farmed)
Multiple
losses and Clinical disease observed in herd
Isolated in
2007 |
|
Virus ID:
07-517030
Tube labelled:
517030; IVD
BHK1; 10-30-07
(sample prepared
30th Oct 2007) |
Isolated from spleen sample
E1/BHK1 |
virus isolate
supplied by / Reagent also
available from:
:
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA
nvsluf@aphis.usda.gov
|
Technical
contact in USA:
Donna Johnson
:
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
|
|
USA2006/11
|
BTV- 3
Initial
attempts to type the virus at SCWDS were unsuccessful.
Virus serotype
identified by RT-PCR using type
specific primers and sequencing (Seg-2)
[1]. Confirmed by SNT at NVSL.
|
USA: Yalobusha
County
Sample taken in the fall of 2006 |
White tailed
deer
Multiple
losses and Clinical disease observed in herd
Isolated at
the Southeastern Cooperative Wildlife Disease Study (SCWDS) Athens
GA. |
Shelf A15,
box A15 γ,
rows
I1-J10
(Added to collection
16th Oct 2008) |
Virus ID:06-473906-7
Tube labelled:
473906-7; orig
isolate to BHK/2; 1-3-07
(sample prepared
3rd Jan 2007) |
Isolated from tissue sample
CPAE 1/BHK2 |
virus isolate
supplied by / Reagent also
available from:
:
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA
nvsluf@aphis.usda.gov
|
Technical
contact in USA:
Donna Johnson
:
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
(82% similar
(Seg-2) to other BTV-3 isolates from Florida
(1130 bp
region)) |
|
USA2006/10
|
BTV-3
(Seg-2 RT-PCR
and sequencing, suggests BTV-3, however only 1 set of BTV-3 specific
primers out of 5 gave +ve amplification. 82% sequence homology was
detected in 675bp of Seg-2 to FL isolates of BTV-3 (which show 91-98%
homology to each other). This places the virus as BTV-3 (Maan
et al 2007)
A virus
neutralisation assay also indicated
BTV-3
although the
type specificity of the BTV-3 sera is unconfirmed.
Further
confirmation is requested |
Yalobusha County,
MS,
USA
(sample taken
during the fall of 2006) |
From White
tailed deer
(no clinical
history)
Date of virus
preparation in USA
3rd Jan 2007 |
Shelf E2,
box E2 γ,
rows A1-B10
Added to
collection
13th Sep 2007 |
06-473906-7
(tube
labelled: 473906-7;
orig isolate
to BHK/2;
1-3-078) |
From blood
*CPAE1/BHK2
[IAH/BHK4 ]
* Note: the
CPAE cell cultures used for isolation are infected with bovine viral
diarrhoea (BVD) virus |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
Technical
contact in USA:
Donna Johnson
Equine
and Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
Original
isolate was submitted from the Southeastern Cooperative Wildlife Disease
Study (SCWDS) Athens GA. isolate obtained from tissues samples collected
in the fall of 2006 in Yalobusha County MS. At SCWDS, samples were
innoculated into cattle pulmonary artery endothelial (CPAE) cells
cultures. sample sent to NVSL for typing. |
|
FRA2003/01 |
BTV-3 |
France |
|
Shelf E3,
box E3 α,
rows E1 to F10 |
|
|
|
|
|
REU2003/02
|
BTV-3
|
from
“la Réunion”
2003 |
from an un-vaccinated cow
|
Shelf C9,
box C9
β
,
rows E1-F10
Added to collection
13th Sept
2010
|
Original tube marked as:
BTV3 Reunion 85/05
|
E1BHK2
[IAH/BHK1 ]
|
Supplied by
Emmanuel Breard,
AFSSA-Maisons
Alfort-France
21st July 2010 |
MTA
[Seg-2: ]
|
|
REU2003/01 |
BTV-3 |
from
“la Réunion”
2003 |
isolated at
AFSSA- Maisons Alfort France
in
2003 |
Shelf E3,
box E3 α,
rows
E1-F10
Added to
collection
13th Sep 2007 |
1567 W1B2: BTV3
|
E1/BHK2
[IAH/BHK2] |
Supplied by Stephan Zientara and
Emmanuel Breard,
AFSSA-Maisons
Alfort-France |
|
|
USA2003/07
|
BTV-3
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-3 antisera). |
Manatee County, FL,
USA
(sample taken
21th Oct 2003) |
From cattle
(no clinical
history)
Date of virus
preparation in USA
12th Nov 2003 |
Shelf E3,
box
E3 α,
rows A1-B10
Added to
collection
13th Sep 2007 |
04-280559-9
(tube
labelled: 280559-9;
IVD YSD BHK/1;
11-12-03) |
From blood
E1/BHK1
[IAH/ BHK3] |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
Multiple
serotypes of BTV were isolated from this herd, including BTV2, 3, 10 (2
isolates), 5,
10 and 19. See
also
USA2003/06
|
|
USA2003/06
|
BTV-3
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-3 antisera) |
Manatee County, FL,
USA
(sample taken
21th Oct 2003) |
From cattle
(no clinical
history)
Date of virus
preparation in USA
2nd Dec 2003 |
Shelf E2,
box E2 γ,
rows I1-J10
Added to
collection
13th Sep 2007 |
04-280559-1
(tube
labelled: 280559-1;
IVD YSD BHK/1;
12-2-03 BT) |
From blood
E1/BHK1
[IAH/BHK3 ] |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
Multiple
serotypes of BTV were isolated from this herd, including BTV2, 3, 10 (2
isolates), 5,
10 and 19.
See also
USA2003/07
|
|
ZIM2002/01
|
BTV
Type 3
|
Zimbabwe
(10/08/02) |
Cattle
from animal
number 0505
|
Shelf A6,
Box A6γ
Rows G1-H10 |
(R11/02 (24))
|
E1/BHK1
[IAH-BHK1] |
aliquots 1/10a,
1/10b pDam, Sent to Kiki Nomikou, Athens Vet Labs , Greece, Oct 2004 |
*
Tube
G1 Freezer A given to Elizabeth Morecroft (Pirbright)
9th Jul 2013.
*
[S2:
S6:AJ586698]
|
|
ZIM2002/02
|
BTV
Type 3
|
Zimbabwe
(22/08/02) |
Cattle
from animal number 0510 |
shelf
C5 box C5α
rows C1-D10
(Added to
collection on
22nd Dec 2005) |
(R11/02 (31))
|
E1/BHK1
[IAH/BHK2] |
|
|
|
USA2002/05
|
BTV-3
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-3 antisera) |
Ocheechobee County, FL,
USA
(sample taken
30th Oct 2002) |
From cattle
(no clinical
history)
Date of virus
preparation in USA
7h Aug 2006 |
Shelf E2,
box E2 γ,
rows
E1-F10
Added to collection
13th Sep
2007
|
03-220082-16
(tube
labelled: 220082-16;
IVD BHK/1;
8-7-06) |
From blood
E1/BHK1/E1BHK1
[IAH/BHK3 ]
(note: repeated passage of the original virus gave low
titres, so virus was re-inoculated into eggs (July 2006) followed by one
pass in BHK cells) |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
Multiple
serotypes of BTV were isolated from this herd, including BTV3, 10 (3
isolates), and 22.
Sample
originally submitted for export testing
|
|
USA2002/04
|
BTV-3
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-3 antisera) |
Alachua County, FL,
USA
(sample taken
23rdAug 2002) |
From Deer
(no clinical
history)
Date of virus
preparation in USA
8th Aug 2006 |
Shelf E2,
box E2 β,
rows
E1-F10
Added to collection
13th Sep
2007
|
02-202795
(202795;
IVD BHK/1;
8-8-06) |
From blood
E1/BHK1/E1BHK1
[IAH/ BHK3]
(note: repeated passage of the original virus gave low
titres, so virus was re-inoculated into eggs (July 2006) followed by one
pass in BHK cells) |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA. |
|
USA2001/15
|
BTV-3
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-3 antisera) |
Martin
County, FL,
USA
(sample taken
5-6th Nov 2001) |
From Deer
(no clinical
history)
Date of virus
preparation in USA 25th Aug 2006 |
Shelf E2,
box E2 γ,
rows
C1-D10
Added to collection
13th Sep
2007
|
02-138555-43
(138555-43; IVD BHK/1;
+BT; 8-25-06) |
From spleen
E1/BHK3/E1BHK1
[IAH/ BHK2]
(note: repeated passage of the original virus gave low
titres, so virus was re-inoculated into eggs (July 2006) followed by one
pass in BHK cells) |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
Samples from
3 deer were originally submitted to USDA. BTV-3 was isolated from
all 3 animals |
|
USA2001/14 |
BTV-3
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-3 antisera) |
Martin
County, FL,
USA
(sample taken
5-6th Nov 2001) |
From Deer
(no clinical
history)
Date of virus
preparation in USA 9th Aug 2006 |
Shelf E3,
box E3 α,
rows
C1-D10
Added to collection
13th Sep
2007
|
02-138555-30
(138555-30;
IVD BHK/1;
8-9-06) |
From Blood
E1/BHK3/E1BHK1
[IAH/BHK3 ]
(note: repeated passage of the original virus gave low
titres, so virus was re-inoculated into eggs (July 2006) followed by one
pass in BHK cells) |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
Samples from
3 deer were originally submitted to USDA. BTV-3 was isolated from
all 3 animals |
|
USA1999/07 |
BTV-3
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-3 antisera) |
Highlands
County, FL,
USA
(sample taken
Dec 1999) |
From sheep
(clinical
signs of BT)
Date of virus
preparation in USA 7th Aug 2006 |
Shelf E2,
box E2 γ,
rows
G1-H10
Added to collection
13th Sep
2007
|
00-22364-10
(22364-10; IVD
BHK1; 8-7-06) |
From Blood
E1/BHK1/E1BHK1
[IAH/ BHK3]
(note: repeated passage of the original virus gave low
titres, so virus was re-inoculated into eggs (July 2006) followed by one
pass in BHK cells) |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA
See also
USA1999/06 |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
Note: BTV-2
was identified in an animal from this flock in November 1999. This
was the first isolation of BTv-2 in the USA for over 16 years.
the flock was comprised of ~1000 sheep that had moved from New Hampshire
to Florida in the fall of 1999. numerous animals were exhibiting
clinical symptoms of BT. Multiple serotypes of BT virus were isolted
from this flock (BTV-2, 3, 13 amd 17) |
|
USA1999/06 |
BTV-3
(typed
by
Seg-2 RT-PCR
and sequencing, confirmed by neutralisation by BTV-3 antisera) |
Highlands
County, FL,
USA
(sample taken
Dec 1999) |
From sheep
(clinical
signs of BT)
Date of virus
preparation in USA 7th Aug 2006 |
Shelf E2,
box E2 β,
rows
A1-B10
Added to collection
13th Sep
2007
|
00-22364-4
(22364-4; IVD
BHK1; 8-7-06) |
From Blood
E1/BHK1/E1BHK1
[IAH/ BHK3]
(note: repeated passage of the original virus gave low
titres, so virus was re-inoculated into eggs (July 2006) followed by one
pass in BHK cells) |
virus isolate
supplied by
USDA, Aphis,
VS, NVSL, PAS
Reagents
Office.
PO box 844
Ames .IA 50010
USA
See also
USA1999/07 |
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844,
Ames IA, 50010, USA.
Note: BTV-2
was identified in an animal from this flock in November 1999. This
was the first isolation of BTv-2 in the USA for over 16 years.
the flock was comprised of ~1000 sheep that had moved from New Hampshire
to Florida in the fall of 1999. numerous animals were exhibiting
clinical symptoms of BT. Multiple serotypes of BT virus were isolted
from this flock (BTV-2, 3, 13 amd 17) |
|
TAT1990/03
|
BTV-3
Initial
attempts to identify serotype by SNT were unsuccessful in 1990
Identified as type
3 by
specific RT-PCR and sequencing (Seg-2)
March 2008
[1] |
Trinidad:
St. Andrews
Sample taken
4th Sept 1990 |
Water buffalo blood
Isolated in
1990 |
Shelf A15,
box A15 γ,
rows
E1-F10
(Added to collection
16th Oct 2008) |
Virus ID:
90-44159-57
Tube labelled:
90-44159-57; YSD BHK/5 -
CPAE1 - BHK2 3-12-08
(sample prepared
12th Mar 2008) |
E1/BHK5/
CPAE1/BHK2 |
|
Technical
contact in USA:
Donna Johnson
Equine and
Ovine Viruses Section, Diagnostic virus Lab, PO box 844
Ames IA, 50010
USA.
Reagent also
available from:
USDA, Aphis,
VS, NVSL, PAS, Attn: Reagents Office P.O. Box 844 Ames, IA 50010 USA
nvsluf@aphis.usda.gov
|
|
AUS1988/01
|
BTV-3
|
Australia
(1988)
|
|
|
Old isolate No.: Y919
Origin: DPP973
Sample sent as 'BTV-3
|
??/2BHK21 (PP)
[IAH/??]
|
Peter Kirkland,
Elizabeth Macarthur Agricultural Institute,
Menangle, NSW 2568 Australia
Sent: 7th Sept 2010
|
Note
supplied with sample: CPE positive / BT-PCR positive
It is uncertain if the date given is sampling date or date of arrival at
EMAI.
ex BARC
(MTA) |
|
AUS1986/01 |
BTV-3
|
Australia,
Northern Territories
1986 |
(G. Gard) |
Shelf A6,
Box A6γ
Rows I1-J10
|
(DPP973,
8805-25-1000) |
(E1/BHK3)
[IAHBHK3] |
Dr. Ian Pritchard
AAHL Geelong,
Australia |
*
Vial position I1 removed from Freezer A and
given to Elizabeth Morecroft (Pirbright) 9th Jul 2013.
*
*MTA* , not to be supplied
to 3rd parties without written
consent from original source
|
|
NIG1982/06 |
BTV
Type 3
|
Nigeria
(26/08/82) |
NK
(animal No.
0510) |
Shelf A6,
Box A6γ
Rows A1-B10 |
(N-348)
|
E1/BHK3
[BHK-1] |
|
Tube B1 (freezer A&B) sent to
Kiki Nomikou, Athens Vet lab, Greece,
Oct 2004
*
TubeA1 from Freezer A given to Elizabeth Morecroft (Pirbright)
9th July 2013.
*
» contents
|
|
CYP1958/02 |
BTV-3 |
|
|
Shelf D9,
box D9 α,
rows C1 to D10 |
|
Virus isolate |
|
|
|
CYP1958/01 |
BTV-3 |
|
|
Shelf D9,
box D9 α,
rows A1 to B10, |
|
Virus isolate |
|
|
